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GB1302706A - - Google Patents

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Publication number
GB1302706A
GB1302706A GB1302706DA GB1302706A GB 1302706 A GB1302706 A GB 1302706A GB 1302706D A GB1302706D A GB 1302706DA GB 1302706 A GB1302706 A GB 1302706A
Authority
GB
United Kingdom
Prior art keywords
column
enzyme
insolubilized
insoluble support
enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed filed Critical
Publication of GB1302706A publication Critical patent/GB1302706A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
    • C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
    • C12N11/06—Enzymes or microbial cells immobilised on or in an organic carrier attached to the carrier via a bridging agent

Landscapes

  • Zoology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)

Abstract

1302706 Insolubilized enzymes; polymer supports NATIONAL RESEARCH DEVELOPMENT CORP 14 Aug 1970 [23 July 1969] 37111/69 Headings C3H and C3R A process for the production of an insolubilized enzyme comprises chemically treating an insoluble support in the form of a column of permeable polymeric material to produce a stable intermediate containing reactive bridging groups, and then flowing a solution of an enzyme through the column so that the enzyme reacts with the reactive bridging groups so formed and becomes attached to the insoluble support. The column may be of a natural or synthetic polymer; the treatment may introduce azide or triazinyl groups. In examples DEAE-cellulose, foam polyester and foam polyurethane are treated with aminodichloro-striazine, Enzymes mentioned are trypsin, chymotrypsin, papain, #-galactosidase, ribonuclease, alkaline phosphatase, amyloglycosidase, dextranase, lactic dehydrogenase, creatine phosphokinase, pyruvate kinase, glucose oxidase, amidase and penicillinamidase.
GB1302706D 1969-07-23 1969-07-23 Expired GB1302706A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB3711169 1969-07-23

Publications (1)

Publication Number Publication Date
GB1302706A true GB1302706A (en) 1973-01-10

Family

ID=10393823

Family Applications (1)

Application Number Title Priority Date Filing Date
GB1302706D Expired GB1302706A (en) 1969-07-23 1969-07-23

Country Status (1)

Country Link
GB (1) GB1302706A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2381059A1 (en) * 1977-02-17 1978-09-15 Sumitomo Chemical Co PROCESS FOR THE PREPARATION OF A FIXED ENZYME, THE FIXING VEHICLE BEING A PULLULANE GEL
FR2433183A1 (en) * 1978-08-07 1980-03-07 Grace W R Ltd ENZYMATIC DIAGNOSTIC COMPOSITION, METHOD FOR ANALYZING BODILY FLUIDS FROM HUMANS, AND METHOD FOR BINDING ENZYMES USING THE SAME
US4371611A (en) 1978-08-07 1983-02-01 W. R. Grace & Co. Enzymatic diagnostic composition

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2381059A1 (en) * 1977-02-17 1978-09-15 Sumitomo Chemical Co PROCESS FOR THE PREPARATION OF A FIXED ENZYME, THE FIXING VEHICLE BEING A PULLULANE GEL
FR2433183A1 (en) * 1978-08-07 1980-03-07 Grace W R Ltd ENZYMATIC DIAGNOSTIC COMPOSITION, METHOD FOR ANALYZING BODILY FLUIDS FROM HUMANS, AND METHOD FOR BINDING ENZYMES USING THE SAME
US4371611A (en) 1978-08-07 1983-02-01 W. R. Grace & Co. Enzymatic diagnostic composition

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Legal Events

Date Code Title Description
PS Patent sealed
429A Application made for amendment of specification (sect. 29/1949)
429H Application (made) for amendment of specification now open to opposition (sect. 29/1949)
429D Case decided by the comptroller ** specification amended (sect. 29/1949)
PCNP Patent ceased through non-payment of renewal fee