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GB1208398A - Reagent for assaying creatine phosphokinase - Google Patents

Reagent for assaying creatine phosphokinase

Info

Publication number
GB1208398A
GB1208398A GB09765/69A GB1976569A GB1208398A GB 1208398 A GB1208398 A GB 1208398A GB 09765/69 A GB09765/69 A GB 09765/69A GB 1976569 A GB1976569 A GB 1976569A GB 1208398 A GB1208398 A GB 1208398A
Authority
GB
United Kingdom
Prior art keywords
glucose
creatine
adp
tpn
pdh
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB09765/69A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EMD Chemicals Inc
Original Assignee
Calbiochem Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US561757A external-priority patent/US3413198A/en
Application filed by Calbiochem Corp filed Critical Calbiochem Corp
Publication of GB1208398A publication Critical patent/GB1208398A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/008—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions for determining co-enzymes or co-factors, e.g. NAD, ATP
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/902—Oxidoreductases (1.)
    • G01N2333/904—Oxidoreductases (1.) acting on CHOH groups as donors, e.g. glucose oxidase, lactate dehydrogenase (1.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1,208,398. Determination of creatine phosphokinase. CALBIOCHEM. 17 April, 1969 [14 June, 1968), No. 19765/69. Addition to 1,163,409. Heading B1X. A substantially anhydrous solid assay material for dissolving in water for determination of creatine phosphokinate (CPK) comprises a mixture of the substrates creatine phosphate and glucose, the enzymes hexokinase and glucose 6-phosphate dehydrogenase (G-6-PDH), the coenzymes adenosine diphosphate (ADP) and triphosphopyridine nucleotide (TPN), a buffer to maintain the pH between 6.5 and 7.5, a stabiliser which is a disulphide compound other than cysteine and optionally one or more of the stabilisers acacia, tris(hydroxymethyl) aminomethane-sulphate and ammonium sulphate, and an activator comprising a water soluble magnesium salt, for example magnesium aspartate. The disulphide stabiliser may be dithiothreitol or dithioerythritol. In the test system the reactions are:- creatine phosphate + ADP CPK creatine + ATP glucose + # ATP hexokinase glucose-6-P + ADP glucose # -6-P + TPN G-6-PDH 6-phosphogluconate + # TPNH. The rate of change of optional density in the system is measured.
GB09765/69A 1966-06-30 1969-04-17 Reagent for assaying creatine phosphokinase Expired GB1208398A (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US561757A US3413198A (en) 1966-06-30 1966-06-30 Reagents and method for assaying biological samples
US73697668A 1968-06-14 1968-06-14

Publications (1)

Publication Number Publication Date
GB1208398A true GB1208398A (en) 1970-10-14

Family

ID=27072744

Family Applications (1)

Application Number Title Priority Date Filing Date
GB09765/69A Expired GB1208398A (en) 1966-06-30 1969-04-17 Reagent for assaying creatine phosphokinase

Country Status (2)

Country Link
US (1) US3540984A (en)
GB (1) GB1208398A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994017794A1 (en) * 1993-02-03 1994-08-18 Steven Andrew Jennings Blends of glycine derivatives and sugars

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3926727A (en) * 1971-09-24 1975-12-16 Choay Sa Urokinase preparations
GB1383216A (en) * 1972-05-03 1975-02-05 Polymeric Enzymes Inc Stabilization of enzymes
JPS5137343B2 (en) * 1972-12-07 1976-10-15
US3956069A (en) * 1974-04-29 1976-05-11 Abbott Laboratories Enzymatic assays for glucose, creatine phosphokinase or plasma ammonia
US3929580A (en) * 1974-08-29 1975-12-30 American Cyanamid Co Creatine phosphokinase test indicator
US3994783A (en) * 1975-09-26 1976-11-30 Calbiochem Differential assay of creatine phosphokinase isoenzymes
US4042462A (en) * 1975-12-24 1977-08-16 Monsanto Company Creatine phosphokinase determination method
US4277562A (en) * 1976-09-13 1981-07-07 Modrovich Ivan Endre Stabilized liquid enzyme and coenzyme compositions
US4132598A (en) * 1977-06-03 1979-01-02 Modrovich Ivan Endre Stabilized liquid phosphate containing diagnostic compositions and method of preparing same
US4127502A (en) * 1977-06-10 1978-11-28 Eastman Kodak Company Stabilizers for reconstituted, lyophilized samples
DE2831390C2 (en) * 1978-07-17 1980-08-14 Boehringer Mannheim Gmbh, 6800 Mannheim Control serum for clinical-chemical analysis with a certain content of creatine kinase
US4250254A (en) * 1978-09-11 1981-02-10 Modrovich Ivan Endre Stabilized liquid enzyme and coenzyme compositions
US4247633A (en) * 1979-04-05 1981-01-27 Pabst Brewing Company Reagent for colorimetric determination of creative phosphokinase
US4229369A (en) * 1979-04-25 1980-10-21 Hycel, Inc. Tris (hydroxymethyl) aminomethane salt of 2-mercaptosuccinic acid
US4492753A (en) * 1982-10-06 1985-01-08 Immudx, Inc. Method and means for assessment and prediction of risk of subsequent ischemic cardiac events
US6290991B1 (en) 1994-12-02 2001-09-18 Quandrant Holdings Cambridge Limited Solid dose delivery vehicle and methods of making same

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3072532A (en) * 1958-11-04 1963-01-08 Innerfield Irving Administration of enzymic composition
US3133001A (en) * 1959-11-26 1964-05-12 Muset Pedro Puig Stabilization of enzymes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994017794A1 (en) * 1993-02-03 1994-08-18 Steven Andrew Jennings Blends of glycine derivatives and sugars

Also Published As

Publication number Publication date
US3540984A (en) 1970-11-17

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PE20 Patent expired after termination of 20 years