GB1065127A - Pituitary gonadotropic hormone preparation and method for its production - Google Patents
Pituitary gonadotropic hormone preparation and method for its productionInfo
- Publication number
- GB1065127A GB1065127A GB41811/63A GB4181163A GB1065127A GB 1065127 A GB1065127 A GB 1065127A GB 41811/63 A GB41811/63 A GB 41811/63A GB 4181163 A GB4181163 A GB 4181163A GB 1065127 A GB1065127 A GB 1065127A
- Authority
- GB
- United Kingdom
- Prior art keywords
- precipitate
- ethanol
- active material
- ammonium acetate
- aqueous
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 230000001456 gonadotroph Effects 0.000 title abstract 3
- 229940088597 hormone Drugs 0.000 title abstract 3
- 239000005556 hormone Substances 0.000 title abstract 3
- 230000001817 pituitary effect Effects 0.000 title abstract 2
- 238000004519 manufacturing process Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 abstract 9
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 abstract 4
- 239000002244 precipitate Substances 0.000 abstract 4
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 abstract 3
- 239000005695 Ammonium acetate Substances 0.000 abstract 3
- 102000009151 Luteinizing Hormone Human genes 0.000 abstract 3
- 108010073521 Luteinizing Hormone Proteins 0.000 abstract 3
- 239000011149 active material Substances 0.000 abstract 3
- 229940043376 ammonium acetate Drugs 0.000 abstract 3
- 235000019257 ammonium acetate Nutrition 0.000 abstract 3
- 229940040129 luteinizing hormone Drugs 0.000 abstract 3
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 abstract 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract 2
- 239000007787 solid Substances 0.000 abstract 2
- 239000006228 supernatant Substances 0.000 abstract 2
- 210000002700 urine Anatomy 0.000 abstract 2
- JVIPLYCGEZUBIO-UHFFFAOYSA-N 2-(4-fluorophenyl)-1,3-dioxoisoindole-5-carboxylic acid Chemical compound O=C1C2=CC(C(=O)O)=CC=C2C(=O)N1C1=CC=C(F)C=C1 JVIPLYCGEZUBIO-UHFFFAOYSA-N 0.000 abstract 1
- 239000005995 Aluminium silicate Substances 0.000 abstract 1
- VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 abstract 1
- 229920001425 Diethylaminoethyl cellulose Polymers 0.000 abstract 1
- 239000005909 Kieselgur Substances 0.000 abstract 1
- 229920005654 Sephadex Polymers 0.000 abstract 1
- 239000012507 Sephadex™ Substances 0.000 abstract 1
- 229910000323 aluminium silicate Inorganic materials 0.000 abstract 1
- 235000012211 aluminium silicate Nutrition 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N ammonia Natural products N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 150000001768 cations Chemical class 0.000 abstract 1
- 238000005119 centrifugation Methods 0.000 abstract 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
- 230000003325 follicular Effects 0.000 abstract 1
- NLYAJNPCOHFWQQ-UHFFFAOYSA-N kaolin Chemical compound O.O.O=[Al]O[Si](=O)O[Si](=O)O[Al]=O NLYAJNPCOHFWQQ-UHFFFAOYSA-N 0.000 abstract 1
- 239000008363 phosphate buffer Substances 0.000 abstract 1
- 239000000047 product Substances 0.000 abstract 1
- 230000004936 stimulating effect Effects 0.000 abstract 1
- 210000004291 uterus Anatomy 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- A61K38/17—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- A61K38/22—Hormones
- A61K38/24—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g. HCG; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Endocrinology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Immunology (AREA)
- Reproductive Health (AREA)
- Pharmacology & Pharmacy (AREA)
- Zoology (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
Abstract
A pituitary gonadotropic hormone is obtained from menopausal or post-menopausal human urine by acidifying the urine to pH 4-4.5, adding thereto 0.5% by weight thereof of siliceous or diatomaceous earth and 1% by weight of kaolin and separating the solid by centrifugation after agitating for about an hour. Thereafter, the solids are washed (using acidified water of pH about 4.5) and the active material removed therefrom by elution with 1M aqueous ammonia to give an eluate of pH 11 to 11.3. This eluate is adjusted to pH 8.5 to give a precipitate which is removed and the pH of the supernatant is adjusted to 5-5.5 and about 2 volumes of acetone are added and the whole is left overnight. The precipitate obtained is washed (using acetone, ethanol and ether successively), dried and taken up in 70% aqueous alcohol containing about 10% ammonium acetate, from which a precipitate is obtained by addition of ethanol containing 10% ammonium acetate. The precipitate is dissolved in 0.05M phosphate buffer (pH 7.0) and stirred with diethylaminoethyl cellulose. The supernatant is acidified to pH 5.4 and passed through a synthetic alumino-silicate gel cation exchanger column from which the active material is subsequently eluted with 40% aqueous ethanol containing 10% ammonium acetate and precipitated by addition of 95% ethanol. Thereafter, the active material may optionally be further purified by passing an aqueous solution thereof through a "Sephadex" (Registered Trade Mark) column and collecting the first fractions of the effluent. The product comprises follicular stimulating hormone (FSH) and luteinizing hormone (LH) having an FSH:LH activity ratio of at least 3:1 and has a total gonadotropic activity (determined by the mouse uterus test) of above 80 mg. equivalent I.R.P. per mg.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IT478963 | 1963-03-11 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| GB1065127A true GB1065127A (en) | 1967-04-12 |
Family
ID=11113777
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| GB41811/63A Expired GB1065127A (en) | 1963-03-11 | 1963-10-23 | Pituitary gonadotropic hormone preparation and method for its production |
Country Status (3)
| Country | Link |
|---|---|
| FR (1) | FR3819M (en) |
| GB (1) | GB1065127A (en) |
| NL (1) | NL6402059A (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0119168A2 (en) * | 1983-02-11 | 1984-09-19 | ISTITUTO FARMACOLOGICO SERONO SpA | Mixtures of FSH and LH from pig hypophyses in a definite ratio |
| WO2000063248A2 (en) * | 1999-04-16 | 2000-10-26 | Instituto Massone S.A. | Highly purified gonadotropin compositions and process for preparing them |
| WO2004087213A1 (en) * | 2003-04-02 | 2004-10-14 | Ares Trading S.A. | Liquid pharmaceutical formulations of fsh and lh together with a non-ionic surfactant |
| US7022822B1 (en) * | 1999-04-16 | 2006-04-04 | Instituto Massone S.A. | Highly purified gonadotropin compositions and process for preparing them |
| US7446090B2 (en) | 1998-07-23 | 2008-11-04 | Ares Trading S.A. | FSH formulation |
| US7740884B2 (en) | 2003-06-20 | 2010-06-22 | Ares Trading S.A. | Freeze-dried FSH/LH formulations |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4725579A (en) * | 1985-02-21 | 1988-02-16 | Serono Laboratories, Inc. | Method of in vitro fertilization by a unique combination of gonadotropins |
| AU601421B2 (en) * | 1986-05-02 | 1990-09-13 | Betrola Investments Pty Ltd | Method of regulating animal reproduction |
-
1963
- 1963-10-23 GB GB41811/63A patent/GB1065127A/en not_active Expired
- 1963-10-23 FR FR951556A patent/FR3819M/en not_active Expired
-
1964
- 1964-03-02 NL NL6402059A patent/NL6402059A/xx unknown
Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0119168A3 (en) * | 1983-02-11 | 1987-05-06 | Istituto Farmacologico Serono Spa | Mixtures of fsh and lh from pig hypophyses in a definite ratio |
| US4959354A (en) * | 1983-02-11 | 1990-09-25 | Istituto Farmacologico Serono S.P.A. | Mixtures of FSH and LH from pig hypophyses in a definite ratio |
| EP0119168A2 (en) * | 1983-02-11 | 1984-09-19 | ISTITUTO FARMACOLOGICO SERONO SpA | Mixtures of FSH and LH from pig hypophyses in a definite ratio |
| US7446090B2 (en) | 1998-07-23 | 2008-11-04 | Ares Trading S.A. | FSH formulation |
| US7563763B2 (en) | 1998-07-23 | 2009-07-21 | Ares Trading S.A. | FSH and FSH variant formulations, products and methods |
| WO2000063248A2 (en) * | 1999-04-16 | 2000-10-26 | Instituto Massone S.A. | Highly purified gonadotropin compositions and process for preparing them |
| AU779005B2 (en) * | 1999-04-16 | 2004-12-23 | Instituto Massone S.A. | Highly purified gonadotropin compositions and process for preparing them |
| US7022822B1 (en) * | 1999-04-16 | 2006-04-04 | Instituto Massone S.A. | Highly purified gonadotropin compositions and process for preparing them |
| JP2002542260A (en) * | 1999-04-16 | 2002-12-10 | インスチチュート マッソーネ エス．エイ． | Highly purified gonadotropin compositions and methods for their production |
| WO2000063248A3 (en) * | 1999-04-16 | 2001-02-01 | Inst Massone S A | Highly purified gonadotropin compositions and process for preparing them |
| WO2004087213A1 (en) * | 2003-04-02 | 2004-10-14 | Ares Trading S.A. | Liquid pharmaceutical formulations of fsh and lh together with a non-ionic surfactant |
| EA012565B1 (en) * | 2003-04-02 | 2009-10-30 | Арес Трейдинг С.А. | Liquid pharmaceutical formulations of follicle-stimulating hormone (fsh) and luteinising hormone (lh) comprising non-ionic surfactant |
| US7741268B2 (en) | 2003-04-02 | 2010-06-22 | Ares Trading S.A. | Liquid pharmaceutical formulations of FSH and LH together with a non-ionic surfactant |
| US7740884B2 (en) | 2003-06-20 | 2010-06-22 | Ares Trading S.A. | Freeze-dried FSH/LH formulations |
Also Published As
| Publication number | Publication date |
|---|---|
| FR3819M (en) | 1966-01-10 |
| NL6402059A (en) | 1964-09-14 |
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