GB1059886A - Improvements in or relating to the production of micro-organisms - Google Patents
Improvements in or relating to the production of micro-organismsInfo
- Publication number
- GB1059886A GB1059886A GB49057/62A GB4905762A GB1059886A GB 1059886 A GB1059886 A GB 1059886A GB 49057/62 A GB49057/62 A GB 49057/62A GB 4905762 A GB4905762 A GB 4905762A GB 1059886 A GB1059886 A GB 1059886A
- Authority
- GB
- United Kingdom
- Prior art keywords
- micro
- organism
- hydrocarbon
- growth
- nutrient medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 244000005700 microbiome Species 0.000 title abstract 11
- 229930195733 hydrocarbon Natural products 0.000 abstract 12
- 150000002430 hydrocarbons Chemical class 0.000 abstract 12
- 239000004215 Carbon black (E152) Substances 0.000 abstract 9
- 239000007789 gas Substances 0.000 abstract 6
- 235000015097 nutrients Nutrition 0.000 abstract 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract 5
- 239000008346 aqueous phase Substances 0.000 abstract 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 abstract 4
- 239000001301 oxygen Substances 0.000 abstract 4
- 229910052760 oxygen Inorganic materials 0.000 abstract 4
- 238000000746 purification Methods 0.000 abstract 4
- 239000006071 cream Substances 0.000 abstract 2
- 238000000926 separation method Methods 0.000 abstract 2
- 238000005406 washing Methods 0.000 abstract 2
- 241000235015 Yarrowia lipolytica Species 0.000 abstract 1
- 239000007864 aqueous solution Substances 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 239000002054 inoculum Substances 0.000 abstract 1
- 150000002632 lipids Chemical class 0.000 abstract 1
- 239000004094 surface-active agent Substances 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/26—Processes using, or culture media containing, hydrocarbons
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/921—Candida
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/921—Candida
- Y10S435/923—Candida lipolytica
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/8215—Microorganisms
- Y10S435/911—Microorganisms using fungi
- Y10S435/944—Torulopsis
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
A micro-organism, particularly yeast, capable of growth in the presence of a hydrocarbon feedstock consisting wholly or in part of straight chain hydrocarbons in a growth stage is cultivated in the presence of the feedstock, an aqueous nutrient medium and a gas containing free oxygen and subsequently, if necessary, the micro-organism is separated from the bulk of the unconsumed hydrocarbon. The content of straight-chain hydrocarbon in the feedstock is thereby reduced with an improvement in the pour point.ALSO:A micro-organism, particularly yeast, capable of growth in the presence of a hydrocarbon feedstock consisting wholly or in part of straight chain hydrocarbons, in a growth stage is cultivated in the presence of the feedstock, an aqueous nutrient medium and a gas containing free oxygen and subsequently, if necessary, the micro-organism is separated from the bulk of the uncomsumed hydrocarbon whereby there is obtained a micro-organism free of a substantial amount of hydrocarbon but containing a small amount of hydrocarbon as contaminant, and thereafter in a purification stage the micro-organism is maintained with an aqueous nutrient medium and a gas containing free oxygen, whereby the hydrocarbon contaminating the micro-organism is reduced in quantity or is eliminated. The growth stage and purification stage may be carried out continuously in separate fermenters or in different zones of the same fermenter. After the growth stage, the major part of the continuous aqueous phase is first separated by decanting and centrifuging. About 80% of the aqueous phase recovered is returned to the fermenter together with make-up quantities of the necessary nutrients. The micro-organism "cream" also obtained by the separation is then mixed with an aqeuous nutrient medium, which may be the same as or different from the aqueous nutrient medium employed in the growth stage, and is maintained in admixture with a gas containing free oxygen whereby the hydrocarbon contaminating the micro-organism is reduced in quantity or is eliminated. Again, the major part of the continuous aqueous phase is separated with recycle of a proportion of the recovered aqueous phase either to the growth stage or to the purification stage. The micro-organism "cream" obtained by this second separation may be further treated for the removal of traces of oil held to the cells, as by washing with an aqueous solution of a surfactant and thereafter with water. Examples are directed to the growth on heavy-gas oil of Candida lipolytica from an inoculum on mixed C9-C16 normal hydrocarbons, and to a subsequent purification of the yeast under the same culture conditions except for the lack of the heavy-gas oil. The purified yeast was recovered by centrifuging and washing at +5 DEG C, and was then freeze-dried. The dried yeast exhibited a substantially reduced lipid content.
Priority Applications (20)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB49057/62A GB1059886A (en) | 1962-12-31 | 1962-12-31 | Improvements in or relating to the production of micro-organisms |
CH1271866A CH476835A (en) | 1962-12-31 | 1963-12-12 | Process for obtaining microorganisms with the simultaneous elimination, at least in part, of straight chain paraffinic hydrocarbons from the hydrocarbon mixtures in which they are contained |
US330527A US3264196A (en) | 1962-12-31 | 1963-12-16 | Purification of micro-organisms cultivated on hydrocarbon feedstock |
DK133565AA DK108492C (en) | 1962-12-31 | 1963-12-23 | Process for the preparation of a yeast product for use as feed. |
DK603163AA DK117622B (en) | 1962-12-31 | 1963-12-23 | Process for the preparation of a microorganism-containing product for use as feed. |
DK133465AA DK109222C (en) | 1962-12-31 | 1963-12-23 | Process for the complete or partial removal of normal paraffins and olefins having at least 10 carbon atoms from hydrocarbon mixtures. |
DK133765AA DK108532C (en) | 1962-12-31 | 1963-12-23 | Process for removing normal paraffins and olefins having at least 10 carbon atoms from hydrocarbon oils. |
SE14526/63A SE325545B (en) | 1962-12-31 | 1963-12-28 | |
CS415366*1A CS155140B2 (en) | 1962-12-31 | 1963-12-30 | |
BR155791/63A BR6355791D0 (en) | 1962-12-31 | 1963-12-30 | PERFECT PROCESS FOR THE PRODUCTION OF MICRO-ORGANISMS IN A CULTURE MEDIA CONTAINING LINEAR CHAIN HYDROCARBONS |
DE1963B0085070 DE1442067A1 (en) | 1962-12-31 | 1963-12-30 | Process for the production of proteinaceous material |
DE1963B0074851 DE1470507C3 (en) | 1962-12-31 | 1963-12-30 | Cultivation of microorganisms |
AT834065A AT262201B (en) | 1962-12-31 | 1963-12-31 | Process for obtaining a mass of microorganisms |
DK372166AA DK117212B (en) | 1962-12-31 | 1966-07-18 | Process for the preparation of a microorganism-containing product for use as feed. |
DK372266AA DK120582B (en) | 1962-12-31 | 1966-07-18 | Process for the preparation of a microorganism-containing product for use as feed. |
NO00165503A NO127540B (en) | 1962-12-31 | 1966-11-08 | |
SE10256/67A SE324344B (en) | 1962-12-31 | 1967-06-30 | |
FI672578A FI43302C (en) | 1962-12-31 | 1967-09-27 | Method for preparing food yeast |
CY42568A CY425A (en) | 1962-12-31 | 1968-01-08 | Improvements in or relating to the production of micro-organisms |
MY196865A MY6800065A (en) | 1962-12-31 | 1968-12-31 | Improvements in or relating to the production of micro-organisms |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB49057/62A GB1059886A (en) | 1962-12-31 | 1962-12-31 | Improvements in or relating to the production of micro-organisms |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1059886A true GB1059886A (en) | 1967-02-22 |
Family
ID=10450956
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB49057/62A Expired GB1059886A (en) | 1962-12-31 | 1962-12-31 | Improvements in or relating to the production of micro-organisms |
Country Status (11)
Country | Link |
---|---|
US (1) | US3264196A (en) |
AT (1) | AT262201B (en) |
BR (1) | BR6355791D0 (en) |
CS (1) | CS155140B2 (en) |
CY (1) | CY425A (en) |
DK (1) | DK108492C (en) |
FI (1) | FI43302C (en) |
GB (1) | GB1059886A (en) |
MY (1) | MY6800065A (en) |
NO (1) | NO127540B (en) |
SE (1) | SE324344B (en) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3427223A (en) * | 1964-06-10 | 1969-02-11 | Exxon Research Engineering Co | Coagulating microbial cells to enhance their separation |
US3508927A (en) * | 1965-09-15 | 1970-04-28 | Exxon Research Engineering Co | Use of unsaturated organic acids as bacterial growth promoters |
GB1141107A (en) * | 1966-01-26 | 1969-01-29 | Kyowa Hakko Kogyo Company Ltd | Process for producing sugars by fermentation |
GB1168835A (en) * | 1966-05-13 | 1969-10-29 | British Petroleum Co | Improvements in or relating to the production of Micro-Organisms |
GB1168834A (en) * | 1966-05-13 | 1969-10-29 | British Petroleum Co | Improvements in or relating to the production of Micro-Organisms |
US3489648A (en) * | 1966-12-22 | 1970-01-13 | Phillips Petroleum Co | Microbial hydrocarbon consumption |
US3622465A (en) * | 1967-03-10 | 1971-11-23 | Allied Chem | Protein from normal hydrocarbons |
US3620927A (en) * | 1969-06-26 | 1971-11-16 | Gulf Research Development Co | Cultivation of micro-organisms on hydrocarbons |
US3904476A (en) * | 1973-05-29 | 1975-09-09 | Mobil Oil Corp | Treatment of cells of a hydrocarbon-consuming microorganism |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US2697061A (en) * | 1950-08-17 | 1954-12-14 | Texaco Development Corp | Processing of hydrocarbons |
US2697062A (en) * | 1951-03-30 | 1954-12-14 | Texaco Development Corp | Processing of hydrocarbons |
US2742398A (en) * | 1951-06-09 | 1956-04-17 | Texaco Development Corp | Method of removing deposits of wax and like materials |
US2982692A (en) * | 1957-06-26 | 1961-05-02 | Hardin B Mcdill | Dewaxing of oils |
US3069325A (en) * | 1959-12-21 | 1962-12-18 | Phillips Petroleum Co | Treatment of hydrocarbons |
-
1962
- 1962-12-31 GB GB49057/62A patent/GB1059886A/en not_active Expired
-
1963
- 1963-12-16 US US330527A patent/US3264196A/en not_active Expired - Lifetime
- 1963-12-23 DK DK133565AA patent/DK108492C/en active
- 1963-12-30 BR BR155791/63A patent/BR6355791D0/en unknown
- 1963-12-30 CS CS415366*1A patent/CS155140B2/cs unknown
- 1963-12-31 AT AT834065A patent/AT262201B/en active
-
1966
- 1966-11-08 NO NO00165503A patent/NO127540B/no unknown
-
1967
- 1967-06-30 SE SE10256/67A patent/SE324344B/xx unknown
- 1967-09-27 FI FI672578A patent/FI43302C/en active
-
1968
- 1968-01-08 CY CY42568A patent/CY425A/en unknown
- 1968-12-31 MY MY196865A patent/MY6800065A/en unknown
Also Published As
Publication number | Publication date |
---|---|
DK108492C (en) | 1967-12-27 |
MY6800065A (en) | 1968-12-31 |
CY425A (en) | 1968-01-08 |
FI43302B (en) | 1970-11-30 |
FI43302C (en) | 1971-03-10 |
NO127540B (en) | 1973-07-09 |
SE324344B (en) | 1970-06-01 |
US3264196A (en) | 1966-08-02 |
AT262201B (en) | 1968-06-10 |
BR6355791D0 (en) | 1973-08-28 |
CS155140B2 (en) | 1974-05-30 |
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