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FR3127048B1 - Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté - Google Patents

Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté Download PDF

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Publication number
FR3127048B1
FR3127048B1 FR2109516A FR2109516A FR3127048B1 FR 3127048 B1 FR3127048 B1 FR 3127048B1 FR 2109516 A FR2109516 A FR 2109516A FR 2109516 A FR2109516 A FR 2109516A FR 3127048 B1 FR3127048 B1 FR 3127048B1
Authority
FR
France
Prior art keywords
optical
microscope
camera
view
optical system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2109516A
Other languages
English (en)
Other versions
FR3127048A1 (fr
Inventor
Amaury Badon
Gaëlle Recher
Pierre Nassoy
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inst Doptique Theorique Et Appliquee Iota
Centre National de la Recherche Scientifique CNRS
Universite de Bordeaux
Original Assignee
Inst Doptique Theorique Et Appliquee Iota
Centre National de la Recherche Scientifique CNRS
Universite de Bordeaux
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst Doptique Theorique Et Appliquee Iota, Centre National de la Recherche Scientifique CNRS, Universite de Bordeaux filed Critical Inst Doptique Theorique Et Appliquee Iota
Priority to FR2109516A priority Critical patent/FR3127048B1/fr
Priority to PCT/EP2022/075139 priority patent/WO2023036946A1/fr
Priority to EP22777969.1A priority patent/EP4399558A1/fr
Priority to US18/690,668 priority patent/US20240418973A1/en
Publication of FR3127048A1 publication Critical patent/FR3127048A1/fr
Application granted granted Critical
Publication of FR3127048B1 publication Critical patent/FR3127048B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/361—Optical details, e.g. image relay to the camera or image sensor
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/008—Details of detection or image processing, including general computer control
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0036—Scanning details, e.g. scanning stages
    • G02B21/0048—Scanning details, e.g. scanning stages scanning mirrors, e.g. rotating or galvanomirrors, MEMS mirrors
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/002—Scanning microscopes
    • G02B21/0024—Confocal scanning microscopes (CSOMs) or confocal "macroscopes"; Accessories which are not restricted to use with CSOMs, e.g. sample holders
    • G02B21/0052—Optical details of the image generation
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/06—Means for illuminating specimens
    • G02B21/08—Condensers
    • G02B21/088—Condensers for both incident illumination and transillumination
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/18—Arrangements with more than one light path, e.g. for comparing two specimens
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/36—Microscopes arranged for photographic purposes or projection purposes or digital imaging or video purposes including associated control and data processing arrangements
    • G02B21/365—Control or image processing arrangements for digital or video microscopes
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B26/00—Optical devices or arrangements for the control of light using movable or deformable optical elements
    • G02B26/08—Optical devices or arrangements for the control of light using movable or deformable optical elements for controlling the direction of light
    • G02B26/10—Scanning systems
    • G02B26/101—Scanning systems with both horizontal and vertical deflecting means, e.g. raster or XY scanners
    • G—PHYSICS
    • G02—OPTICS
    • G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00—Microscopes
    • G02B21/0004—Microscopes specially adapted for specific applications
    • G02B21/0088—Inverse microscopes

Landscapes

  • Physics & Mathematics (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Multimedia (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • General Engineering & Computer Science (AREA)
  • Microscoopes, Condenser (AREA)

Abstract

L’invention concerne un microscope optique (100) et un procédé de microscopie. Selon l’invention, le microscope comporte un module d’imagerie (200) comprenant un séparateur optique de faisceau (40), un premier système optique (41, 42, 43), une première caméra (51), un second système optique (44, 45, 46, 47) et une seconde caméra (52), le second système optique (44, 45, 46, 47) et la seconde caméra (52) étant configurés pour acquérir une image large vue, et le premier système optique (41, 42, 43) comportant un dispositif réflectif de balayage (42, 48) disposé dans un plan (72) conjugué optiquement avec le plan de Fourier (71) du microscope optique (100), un contrôleur (300) étant adapté pour orienter angulairement le dispositif réflectif de balayage (42, 48) de façon à ce que la première caméra (51) acquière au moins une première image (61, 62,…, 6N) d’une portion du champ objet de l’objectif de microscope (21, 22, 23). Figure pour l’abrégé : Fig. 1
FR2109516A 2021-09-10 2021-09-10 Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté Active FR3127048B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR2109516A FR3127048B1 (fr) 2021-09-10 2021-09-10 Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté
PCT/EP2022/075139 WO2023036946A1 (fr) 2021-09-10 2022-09-09 Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté
EP22777969.1A EP4399558A1 (fr) 2021-09-10 2022-09-09 Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté
US18/690,668 US20240418973A1 (en) 2021-09-10 2022-09-09 Augmented-field-of-view, high-resolution optical microscopy method and optical microscope

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2109516 2021-09-10
FR2109516A FR3127048B1 (fr) 2021-09-10 2021-09-10 Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté

Publications (2)

Publication Number Publication Date
FR3127048A1 FR3127048A1 (fr) 2023-03-17
FR3127048B1 true FR3127048B1 (fr) 2024-07-26

Family

ID=78770736

Family Applications (1)

Application Number Title Priority Date Filing Date
FR2109516A Active FR3127048B1 (fr) 2021-09-10 2021-09-10 Microscope optique et procédé de microscopie optique haute résolution à champ de vue augmenté

Country Status (4)

Country Link
US (1) US20240418973A1 (fr)
EP (1) EP4399558A1 (fr)
FR (1) FR3127048B1 (fr)
WO (1) WO2023036946A1 (fr)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1549992A2 (fr) * 2002-09-16 2005-07-06 Rensselaer Polytechnic Institute Microscope a champ de vision etendu
EP1807724A2 (fr) * 2004-11-02 2007-07-18 Umech Technologies Co. Systeme d'imagerie numerique optiquement ameliore
EP2703871A3 (fr) * 2005-05-25 2014-09-03 Massachusetts Institute Of Technology Systèmes de microscopie multi-focale à balayage et procédes associes
DE102009010446B4 (de) * 2009-02-26 2015-07-09 Carl Zeiss Meditec Ag Verfahren und Vorrichtung zur Quantifizierung des Blutflusses in einem Gewebebereich
JP5371694B2 (ja) * 2009-10-26 2013-12-18 オリンパス株式会社 顕微鏡接続ユニットおよび顕微鏡システム
US10139613B2 (en) * 2010-08-20 2018-11-27 Sakura Finetek U.S.A., Inc. Digital microscope and method of sensing an image of a tissue sample
DE102012023024B4 (de) * 2012-11-07 2023-05-04 Carl Zeiss Microscopy Gmbh Lichtmikroskop und Mikroskopieverfahren
EP3513238B1 (fr) * 2016-09-13 2024-06-26 Inscopix, Inc. Adaptateur pour imagerie microscopique
DE102016120312B3 (de) * 2016-10-25 2017-10-05 Leica Microsystems Cms Gmbh Verfahren zum Beleuchten von Fokuspositionen objektseitig eines Objektivs eines Mikroskops und Mikroskop

Also Published As

Publication number Publication date
FR3127048A1 (fr) 2023-03-17
WO2023036946A1 (fr) 2023-03-16
US20240418973A1 (en) 2024-12-19
EP4399558A1 (fr) 2024-07-17

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