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FR3108124B1 - METHOD FOR DETECTING AND OPTIONALLY QUANTIFYING AN ANALYTE WITH A DOUBLE STEM-LOOP STRUCTURE OLIGONUCLEOTIDE AND SAID OLIGONUCLEOTIDE - Google Patents

METHOD FOR DETECTING AND OPTIONALLY QUANTIFYING AN ANALYTE WITH A DOUBLE STEM-LOOP STRUCTURE OLIGONUCLEOTIDE AND SAID OLIGONUCLEOTIDE Download PDF

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Publication number
FR3108124B1
FR3108124B1 FR2002366A FR2002366A FR3108124B1 FR 3108124 B1 FR3108124 B1 FR 3108124B1 FR 2002366 A FR2002366 A FR 2002366A FR 2002366 A FR2002366 A FR 2002366A FR 3108124 B1 FR3108124 B1 FR 3108124B1
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FR
France
Prior art keywords
oligonucleotide
detecting
analyte
loop structure
optionally quantifying
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR2002366A
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French (fr)
Other versions
FR3108124A1 (en
Inventor
Maud Savonnet
Arnaud Buhot
Myriam-Laure Cubizolles
Yoann Roupioz
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Centre National de la Recherche Scientifique CNRS
Universite Grenoble Alpes
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Centre National de la Recherche Scientifique CNRS
Commissariat a lEnergie Atomique CEA
Universite Grenoble Alpes
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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Application filed by Centre National de la Recherche Scientifique CNRS, Commissariat a lEnergie Atomique CEA, Universite Grenoble Alpes, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA filed Critical Centre National de la Recherche Scientifique CNRS
Priority to FR2002366A priority Critical patent/FR3108124B1/en
Priority to EP21160980.5A priority patent/EP3878971B1/en
Publication of FR3108124A1 publication Critical patent/FR3108124A1/en
Application granted granted Critical
Publication of FR3108124B1 publication Critical patent/FR3108124B1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La présente invention concerne un procédé pour détecter et éventuellement quantifier un analyte éventuellement présent dans un échantillon liquide, ledit procédé mettant en œuvre un oligonucléotide à double structure tige-boucle et une amplification LAMP à deux amorces. La présente invention concerne également des oligonucléotides à double structure tige-boucle particuliers.The present invention relates to a method for detecting and optionally quantifying an analyte possibly present in a liquid sample, said method implementing a dual stem-loop oligonucleotide and a two-primer LAMP amplification. The present invention also relates to particular dual stem-loop oligonucleotides.

FR2002366A 2020-03-10 2020-03-10 METHOD FOR DETECTING AND OPTIONALLY QUANTIFYING AN ANALYTE WITH A DOUBLE STEM-LOOP STRUCTURE OLIGONUCLEOTIDE AND SAID OLIGONUCLEOTIDE Active FR3108124B1 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
FR2002366A FR3108124B1 (en) 2020-03-10 2020-03-10 METHOD FOR DETECTING AND OPTIONALLY QUANTIFYING AN ANALYTE WITH A DOUBLE STEM-LOOP STRUCTURE OLIGONUCLEOTIDE AND SAID OLIGONUCLEOTIDE
EP21160980.5A EP3878971B1 (en) 2020-03-10 2021-03-05 Method for detecting and possibly quantifying an analyte with an oligonucleotide with double stem-loop structure

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
FR2002366A FR3108124B1 (en) 2020-03-10 2020-03-10 METHOD FOR DETECTING AND OPTIONALLY QUANTIFYING AN ANALYTE WITH A DOUBLE STEM-LOOP STRUCTURE OLIGONUCLEOTIDE AND SAID OLIGONUCLEOTIDE
FR2002366 2020-03-10

Publications (2)

Publication Number Publication Date
FR3108124A1 FR3108124A1 (en) 2021-09-17
FR3108124B1 true FR3108124B1 (en) 2025-02-28

Family

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Family Applications (1)

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FR2002366A Active FR3108124B1 (en) 2020-03-10 2020-03-10 METHOD FOR DETECTING AND OPTIONALLY QUANTIFYING AN ANALYTE WITH A DOUBLE STEM-LOOP STRUCTURE OLIGONUCLEOTIDE AND SAID OLIGONUCLEOTIDE

Country Status (1)

Country Link
FR (1) FR3108124B1 (en)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR3131315A1 (en) * 2021-12-27 2023-06-30 Commissariat A L'energie Atomique Et Aux Energies Alternatives LINEAR OLIGONUCLEOTIDE AND USE IN THE DETECTION OF ANALYTES OF INTEREST

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE374833T1 (en) 1998-11-09 2007-10-15 Eiken Chemical METHOD FOR SYNTHESIS OF NUCLEIC ACIDS
CN106148549A (en) 2016-08-31 2016-11-23 湖南大学 A kind of loop-mediated isothermal amplification technique based on connection is for detection method of gene mutation

Also Published As

Publication number Publication date
FR3108124A1 (en) 2021-09-17

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