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FR3088341B1 - Dispositif de culture cellulaire - Google Patents

Dispositif de culture cellulaire Download PDF

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Publication number
FR3088341B1
FR3088341B1 FR1860401A FR1860401A FR3088341B1 FR 3088341 B1 FR3088341 B1 FR 3088341B1 FR 1860401 A FR1860401 A FR 1860401A FR 1860401 A FR1860401 A FR 1860401A FR 3088341 B1 FR3088341 B1 FR 3088341B1
Authority
FR
France
Prior art keywords
culture
cell culture
fluid inlet
culture chamber
culture device
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR1860401A
Other languages
English (en)
Other versions
FR3088341A1 (fr
Inventor
Mikhael Hadida
David Marchat
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institut Mines Telecom IMT
Original Assignee
Institut Mines Telecom IMT
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institut Mines Telecom IMT filed Critical Institut Mines Telecom IMT
Priority to FR1860401A priority Critical patent/FR3088341B1/fr
Priority to EP19818234.7A priority patent/EP3877500A1/fr
Priority to PCT/FR2019/052669 priority patent/WO2020095005A1/fr
Priority to US17/292,245 priority patent/US20210395662A1/en
Publication of FR3088341A1 publication Critical patent/FR3088341A1/fr
Application granted granted Critical
Publication of FR3088341B1 publication Critical patent/FR3088341B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/14—Scaffolds; Matrices
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/22—Transparent or translucent parts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/48—Holding appliances; Racks; Supports
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Dispositif de culture et/ou d'ensemencement cellulaire (10) comportant : - au moins une chambre de culture présentant au moins une fenêtre optique transparente (11), - au moins une entrée de fluide (12) et au moins une sortie de fluide (13) communiquant avec la chambre de culture, - au moins un support de culture tridimensionnel (20) disposé dans la chambre de culture, présentant des surfaces (21, 22) espacées le long d'un axe (X) du support de culture, le support de culture étant disposé dans la chambre de culture de manière à être traversé d'une surface à l'autre par l'écoulement circulant entre l'entrée de fluide et la sortie de fluide, et de manière à ce que l'une de ses surfaces, de préférence la face d'entrée du fluide, soit située au moins partiellement en regard de la fenêtre optique (11).
FR1860401A 2018-11-09 2018-11-09 Dispositif de culture cellulaire Active FR3088341B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR1860401A FR3088341B1 (fr) 2018-11-09 2018-11-09 Dispositif de culture cellulaire
EP19818234.7A EP3877500A1 (fr) 2018-11-09 2019-11-08 Dispositif de culture cellulaire
PCT/FR2019/052669 WO2020095005A1 (fr) 2018-11-09 2019-11-08 Dispositif de culture cellulaire
US17/292,245 US20210395662A1 (en) 2018-11-09 2019-11-08 Cell Culture Device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR1860401A FR3088341B1 (fr) 2018-11-09 2018-11-09 Dispositif de culture cellulaire

Publications (2)

Publication Number Publication Date
FR3088341A1 FR3088341A1 (fr) 2020-05-15
FR3088341B1 true FR3088341B1 (fr) 2023-10-06

Family

ID=66166073

Family Applications (1)

Application Number Title Priority Date Filing Date
FR1860401A Active FR3088341B1 (fr) 2018-11-09 2018-11-09 Dispositif de culture cellulaire

Country Status (4)

Country Link
US (1) US20210395662A1 (fr)
EP (1) EP3877500A1 (fr)
FR (1) FR3088341B1 (fr)
WO (1) WO2020095005A1 (fr)

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9388374B2 (en) * 2005-07-07 2016-07-12 Emd Millipore Corporation Microfluidic cell culture systems
US20110091926A1 (en) * 2008-03-25 2011-04-21 Novatissue Gmbh Perfusable bioreactor for the production of human or animal tissues
US20110229970A1 (en) 2010-03-05 2011-09-22 Florida State University Research Foundation Dual-chamber perfusion bioreactor for orthopedic tissue interfaces and methods of use
EP2468846A1 (fr) 2010-12-22 2012-06-27 Ludwig-Maximilians-Universität München Procédé pour examiner la croissance tissulaire et le conditionnement de cellules sur un support et bioréacteur à perfusion
WO2013086284A1 (fr) * 2011-12-09 2013-06-13 University Of Florida Research Foundation, Inc. Cuves à circulation, procédé d'utilisation de la cuve à circulation, et procédés de fabrication de la cuve à circulation
PT106083A (pt) * 2012-01-05 2013-07-05 A4Tec Ass For The Advancement Of Tissue Engineering And Cell Based Technologies & Therapies Bioreactor composto por câmara estanque e matriz interna para geração de implantes médicos celularizados
DE102012105540A1 (de) * 2012-06-26 2014-04-24 Karlsruher Institut für Technologie Gefäßmodell, Verfahren zu seiner Herstellung und seine Verwendung
AU2014253850B2 (en) 2013-04-18 2019-05-16 The Trustees Of Columbia University In The City Of New York Perfusion bioreactor with tissue flow control and live imaging compatibility
EP3114207A4 (fr) 2014-03-03 2017-10-25 Kiyatec Inc. Dispositifs et systèmes de culture de tissus en 3d

Also Published As

Publication number Publication date
EP3877500A1 (fr) 2021-09-15
US20210395662A1 (en) 2021-12-23
FR3088341A1 (fr) 2020-05-15
WO2020095005A1 (fr) 2020-05-14

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