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FR2792832A1 - Preventing cellular aging of the skin using heat shock proteins or heat shock protein inducers isolated from algae - Google Patents

Preventing cellular aging of the skin using heat shock proteins or heat shock protein inducers isolated from algae Download PDF

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Publication number
FR2792832A1
FR2792832A1 FR9905580A FR9905580A FR2792832A1 FR 2792832 A1 FR2792832 A1 FR 2792832A1 FR 9905580 A FR9905580 A FR 9905580A FR 9905580 A FR9905580 A FR 9905580A FR 2792832 A1 FR2792832 A1 FR 2792832A1
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Prior art keywords
heat shock
skin
algae
shock proteins
proteins
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FR9905580A
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French (fr)
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FR2792832B1 (en
Inventor
Antoine Gedouin
Romuald Vallee
Pierre Yves Morvan
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Codif International SA
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Codif International SA
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
    • A61K8/9706—Algae
    • A61K8/9722—Chlorophycota or Chlorophyta [green algae], e.g. Chlorella
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Dermatology (AREA)
  • Epidemiology (AREA)
  • Birds (AREA)
  • Gerontology & Geriatric Medicine (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Medicines Containing Plant Substances (AREA)
  • Cosmetics (AREA)

Abstract

A skin protection method, for preventing cellular aging, involves use of: (i) heat shock proteins (I) isolated from algae; or (ii) molecules (II) isolated from algae which induce constitutive and/or inductive heat shock proteins in the skin cells. Independent claims are included for: (a) a pharmaceutical or cosmetic composition for preventing cellular aging of the skin, containing (I) or (II) as active agent; and (b) the preparation of such compositions, by using an algal extract containing (I) (constitutive and/or inductive) or (II).

Description

La présente invention concerne un procédé de protection de la peau pour laThe present invention relates to a method of protecting the skin for the

prévenir de son vieillissement cellulaire.  prevent cell aging.

Le but de la présente invention est de proposer un tel procédé qui soit nouveau.  The object of the present invention is to propose such a process which is new.

Selon la présente invention, ce procédé consiste à utiliser des protéines de choc thermique isolées d'algues. La présente invention concerne également une composition pharmaceutique ou cosmétique destinée à prévenir le vieillissement cellulaire de la peau qui est caractérisée en ce qu'elle comporte à titre de principe actif des protéines de choc  According to the present invention, this method consists in using heat shock proteins isolated from algae. The present invention also relates to a pharmaceutical or cosmetic composition intended to prevent cellular aging of the skin which is characterized in that it comprises, as active principle, shock proteins

thermique isolées d'algues.thermal isolated from algae.

Les protéines de choc thermique sont des protéines chaperonnes produites par  Heat shock proteins are chaperone proteins produced by

les cellules pour lutter contre des oxydations liées à des dérivés réactifs de l'oxygène.  cells to fight against oxidations linked to reactive oxygen derivatives.

Elles sont connues sous le nom de protéines de choc thermique (Heat Shock Proteins)  They are known as Heat Shock Proteins

ou sous l'abréviation hsp.or under the abbreviation hsp.

Ces protéines sont exprimées dans les cellules cutanées humaines telles que les kératinocytes et les fibroblastes. La production de ces protéines hsp est stimulée par des stress telle qu'une augmentation de température (d'o leur nom), une exposition aux UVB, etc. On sait par ailleurs que les algues expriment des marqueurs biologiques de réponse à un stress. Ces marqueurs sont des protéines qui présentent une structure très proche de celle des protéines hsp de mammifères. Des homologies de séquence ont  These proteins are expressed in human skin cells such as keratinocytes and fibroblasts. The production of these hsp proteins is stimulated by stresses such as an increase in temperature (hence their name), exposure to UVB, etc. We also know that algae express biological markers in response to stress. These markers are proteins which have a structure very close to that of mammalian hsp proteins. Sequence homologies have

montré la présence des gènes codant des protéines hsp dans des algues.  demonstrated the presence of genes encoding hsp proteins in algae.

Il a par exemple été montré que des algues du genre Chlorella exposée à 40 C pendant 30 minutes augmentent la quantité de protéines hsp de 43, 60, 73 et 95 Kda et induisent l'expression d'une protéine hsp 27 Kda. Ces protéines protègent ces algues Chlorella contre une incubation ultérieure à 45 C. On a pu montrer qu'une composition pharmaceutique ou cosmétique dont le principe actif est constitué de protéines de choc thermique isolées d'algues a une  For example, it has been shown that algae of the genus Chlorella exposed to 40 ° C. for 30 minutes increase the quantity of hsp proteins by 43, 60, 73 and 95 Kda and induce the expression of a hsp 27 Kda protein. These proteins protect these Chlorella algae against a subsequent incubation at 45 C. It has been shown that a pharmaceutical or cosmetic composition whose active principle consists of thermal shock proteins isolated from algae has a

influence sur le vieillissement des cellules cutanées.  influence on the aging of skin cells.

Cette influence est corroborée par des études sur le vieillissement qui ont mis en évidence une relation entre l'âge des cellules cutanées humaines et le taux basal de protéines de choc thermique ou entre l'âge des cellules et l'aptitude à exprimer des  This influence is corroborated by studies on aging which have shown a relationship between the age of human skin cells and the basal level of heat shock proteins or between the age of cells and the ability to express

protéines de choc thermique après un choc thermique.  heat shock proteins after heat shock.

2 27928322 2792832

Le procédé de la présente invention consiste par exemple à utiliser en tant que principe actif des protéines de choc thermique constitutives afin d'augmenter le taux  The process of the present invention consists, for example, in using, as active ingredient, constitutive heat shock proteins in order to increase the rate

desdites protéines dans lesdites cellules cutanées.  said proteins in said skin cells.

Mais, il peut également consister à utiliser en tant que principe actif des protéines de choc thermique inductrices afin de stimuler la production desdites  However, it can also consist in using as active principle inducing heat shock proteins in order to stimulate the production of said proteins.

protéines par lesdites cellules cutanées.  proteins by said skin cells.

La présente invention consiste également à utiliser des substances isolées  The present invention also consists in using isolated substances

d'algues et inductrices, dans les cellules de la peau, de protéines de choc thermique.  algae and inducing heat shock proteins in skin cells.

Les protéines de choc thermique induites par lesdites molécules ont ainsi pour effet de protéger les cellules de la peau contre les agressions extérieures et de prévenir  The heat shock proteins induced by said molecules thus have the effect of protecting the skin cells against external aggressions and of preventing

son vieillissement cellulaire.its cellular aging.

La présente invention concerne encore une composition pharmaceutique ou cosmétique pour prévenir le vieillissement cellulaire de la peau qui comporte à titre de principe actif des substances isolées d'algues et inductrices dans lesdites cellules de  The present invention also relates to a pharmaceutical or cosmetic composition for preventing cellular aging of the skin which comprises, as active principle, substances isolated from algae and inducing in said cells.

protéines de choc thermique.heat shock proteins.

Enfin, la présente invention concerne également des procédés d'obtention d'une composition pharmaceutique ou cosmétique destinée à prévenir du vieillissement  Finally, the present invention also relates to methods for obtaining a pharmaceutical or cosmetic composition intended to prevent aging.

cellulaire de la peau.skin cell.

L'un est caractérisé en ce qu'il consiste à utiliser un extrait d'algues contenant  One is characterized in that it consists in using an algae extract containing

des protéines de choc thermique constitutives et/ou inductrices.  constitutive and / or inducing heat shock proteins.

L'autre consiste à utiliser un extrait d'algues contenant des substances inductrices dans lesdites cellules de la peau de protéines de choc thermique  The other is to use an algae extract containing inducing substances in said skin cells of heat shock proteins

constitutives et/ou inductrices.constitutive and / or inductive.

3 27928323 2792832

Claims (7)

REVENDICATIONS 1) Procédé de protection de la peau pour la prévenir de son vieillissement cellulaire, caractérisé en ce qu'il consiste à utiliser des protéines de choc thermique  1) Process for protecting the skin to prevent it from aging in cells, characterized in that it consists in using heat shock proteins isolées d'algues.isolated from algae. 2) Procédé selon la revendication 1, caractérisé en ce qu'il consiste à utiliser des protéines de choc thermique constitutives afin d'augmenter le taux desdites protéines  2) Method according to claim 1, characterized in that it consists in using constitutive heat shock proteins in order to increase the level of said proteins dans lesdites cellules cutanées.in said skin cells. 3) Procédé selon la revendication 1 ou 2, caractérisé en ce qu'il consiste à utiliser des protéines de choc thermique inductrices afin de stimuler la production desdites  3) Method according to claim 1 or 2, characterized in that it consists in using inductive heat shock proteins in order to stimulate the production of said protéines par lesdites cellules cutanées.  proteins by said skin cells. 4) Procédé de protection de la peau pour la prévenir de son vieillissement cellulaire, caractérisé en ce qu'il consiste à utiliser des substances isolées d'algues et inductrices dans lesdites cellules de la peau de protéines de choc thermique  4) A method of protecting the skin to prevent it from aging in cells, characterized in that it consists in using substances isolated from algae and inducing in said skin cells of heat shock proteins constitutives et/ou inductrices.constitutive and / or inductive. ) Procédé d'obtention d'une composition pharmaceutique ou cosmétique destinée à prévenir du vieillissement cellulaire de la peau, caractérisé en ce qu'il consiste à utiliser un extrait d'algues contenant des protéines de choc thermique  ) Process for obtaining a pharmaceutical or cosmetic composition intended to prevent cellular aging of the skin, characterized in that it consists in using an algae extract containing heat shock proteins constitutives et/ou inductrices.constitutive and / or inductive. 6) Procédé d'obtention d'une composition pharmaceutique ou cosmétique destinée à prévenir du vieillissement cellulaire de la peau, caractérisé en ce qu'il consiste à utiliser un extrait d'algues contenant des substances inductrices dans lesdites  6) Process for obtaining a pharmaceutical or cosmetic composition intended to prevent cellular aging of the skin, characterized in that it consists in using an algae extract containing inducing substances in said cellules de la peau de protéines de choc thermique constitutives et/ou inductrices.  constitutive and / or inducing heat shock protein cells. 7) Composition pharmaceutique ou cosmétique destinée à prévenir du vieillissement cellulaire de la peau, caractérisée en ce qu'elle comporte à titre de  7) Pharmaceutical or cosmetic composition intended to prevent cellular aging of the skin, characterized in that it comprises as principe actif des protéines de choc thermique isolées d'algues.  active ingredient in thermal shock proteins isolated from algae. 8) Composition pharmaceutique ou cosmétique destinée à prévenir du vieillissement cellulaire de la peau, caractérisée en ce qu'elle comporte à titre de principe actif des molécules isolées d'algues et inductrices dans lesdites cellules de la  8) Pharmaceutical or cosmetic composition intended to prevent cellular aging of the skin, characterized in that it comprises, as active principle, molecules isolated from algae and inducing in said cells of the peau de protéines de choc thermique constitutives et/ou inductrices.  skin of constituent and / or inducing heat shock proteins.
FR9905580A 1999-04-28 1999-04-28 METHOD FOR PROTECTING THE SKIN TO PREVENT ITS CELL AGING Expired - Fee Related FR2792832B1 (en)

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2809308A1 (en) * 2000-05-29 2001-11-30 Seporga Lab Cosmetic and dermo-pharmaceutical compositions for skin protection containing heat shock proteins and/or heat shock factors
EP1433463A1 (en) * 2002-12-26 2004-06-30 Shirako Co., Ltd. Cosmetics comprising algal proteins
FR2898808A1 (en) * 2006-03-27 2007-09-28 Biotechmarine Soc Par Actions Cosmetic active ingredient consisting of arginine ferrulate and a microalgal extract, useful for fighting against cutaneous aging
WO2010102988A1 (en) * 2009-03-10 2010-09-16 Alfa Biogene International B.V. Skin care product

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2202146A (en) * 1987-03-18 1988-09-21 Caola Kozmetikai Skin-regenerating cosmetic composition
WO1997016439A1 (en) * 1995-11-02 1997-05-09 Biorex Kutató és Fejlesztó Rt. Hydroxylamine derivatives useful for enhancing the molecular chaperon production and the preparation thereof
FR2757863A1 (en) * 1996-12-27 1998-07-03 Inovat Sarl NEW SUBSTANCES WITH BIOLOGICAL ACTIVITY, PROCESS FOR OBTAINING THEM AND COMPOSITIONS COMPRISING THE SAME
WO1998033475A1 (en) * 1997-02-03 1998-08-06 Laboratoires Serobiologiques Active synergetic complex and cosmetic and/or pharmaceutical product containing this complex

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2202146A (en) * 1987-03-18 1988-09-21 Caola Kozmetikai Skin-regenerating cosmetic composition
WO1997016439A1 (en) * 1995-11-02 1997-05-09 Biorex Kutató és Fejlesztó Rt. Hydroxylamine derivatives useful for enhancing the molecular chaperon production and the preparation thereof
FR2757863A1 (en) * 1996-12-27 1998-07-03 Inovat Sarl NEW SUBSTANCES WITH BIOLOGICAL ACTIVITY, PROCESS FOR OBTAINING THEM AND COMPOSITIONS COMPRISING THE SAME
WO1998033475A1 (en) * 1997-02-03 1998-08-06 Laboratoires Serobiologiques Active synergetic complex and cosmetic and/or pharmaceutical product containing this complex

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
H. ARS: "Les Algues, sources d'actifs bio-naturels", PARFUMS, COSMTÉTIQUES, ACTUALITÉS, vol. 134, 1997, pages 55-58, XP002129144 *
H. SHEN: "Thermotolerance induced by heat shock in Chlorella", JOURNAL OF APPLIED PHYCOLOY, vol. 9, no. 5, 1997, pages 471 - 475, XP000870265 *
S. I. RATTAN: "Repeated Mild Heat Shock Delays Ageing in cultured Human Skin Fibroblasts.", BIOCHEMISTRY AND MOLECULAR BIOLOGY INTERNATIONAL, vol. 45, no. 4, July 1998 (1998-07-01), pages 753 - 759, XP002129142 *
X. ZHOU ET AL.: "Heat Shock Transcription Factor-1 Regulates Heat Shock Protein-72 Expression in Human Keratinocytes Exposed to Ultraviolet B light.", JOURNAL OF INVESTIGATIVE DERMATOLOGY, vol. 111, no. 2, August 1998 (1998-08-01), pages 194 - 198, XP002129141 *

Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2809308A1 (en) * 2000-05-29 2001-11-30 Seporga Lab Cosmetic and dermo-pharmaceutical compositions for skin protection containing heat shock proteins and/or heat shock factors
EP1433463A1 (en) * 2002-12-26 2004-06-30 Shirako Co., Ltd. Cosmetics comprising algal proteins
FR2898808A1 (en) * 2006-03-27 2007-09-28 Biotechmarine Soc Par Actions Cosmetic active ingredient consisting of arginine ferrulate and a microalgal extract, useful for fighting against cutaneous aging
WO2007110511A1 (en) * 2006-03-27 2007-10-04 Biotechmarine Societe Par Actions Simplifiee Cosmetic active ingredient composed of arginine ferrulate and a microalgae extract and its uses
AU2007231265B2 (en) * 2006-03-27 2012-03-15 Biotechmarine Cosmetic active ingredient composed of arginine ferrulate and a microalgae extract and its uses
KR101434839B1 (en) 2006-03-27 2014-10-01 바이오테크마린 소시에테 파 액죵 심플리피에 Cosmetic active ingredient composed of arginine ferrulate and a microalgae extract and its uses
WO2010102988A1 (en) * 2009-03-10 2010-09-16 Alfa Biogene International B.V. Skin care product

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