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FR2779154B1 - Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre - Google Patents

Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre

Info

Publication number
FR2779154B1
FR2779154B1 FR9806866A FR9806866A FR2779154B1 FR 2779154 B1 FR2779154 B1 FR 2779154B1 FR 9806866 A FR9806866 A FR 9806866A FR 9806866 A FR9806866 A FR 9806866A FR 2779154 B1 FR2779154 B1 FR 2779154B1
Authority
FR
France
Prior art keywords
primes
amplifying
implementation
nucleotide sequence
particular nucleotide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
FR9806866A
Other languages
English (en)
Other versions
FR2779154A1 (fr
Inventor
Bruno Mougin
Ali Laayoun
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Biomerieux SA
Original Assignee
Biomerieux SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biomerieux SA filed Critical Biomerieux SA
Priority to FR9806866A priority Critical patent/FR2779154B1/fr
Priority to JP2000551041A priority patent/JP2002516666A/ja
Priority to AU39355/99A priority patent/AU3935599A/en
Priority to EP99922230A priority patent/EP1082461A1/fr
Priority to PCT/FR1999/001247 priority patent/WO1999061661A1/fr
Publication of FR2779154A1 publication Critical patent/FR2779154A1/fr
Application granted granted Critical
Publication of FR2779154B1 publication Critical patent/FR2779154B1/fr
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6848Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
FR9806866A 1998-05-27 1998-05-27 Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre Expired - Fee Related FR2779154B1 (fr)

Priority Applications (5)

Application Number Priority Date Filing Date Title
FR9806866A FR2779154B1 (fr) 1998-05-27 1998-05-27 Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
JP2000551041A JP2002516666A (ja) 1998-05-27 1999-05-27 少なくとも一つの特定のヌクレオチド配列の増幅方法及び使用されるプライマー
AU39355/99A AU3935599A (en) 1998-05-27 1999-05-27 Method for amplifying at least a particular nucleotide sequence and primers used
EP99922230A EP1082461A1 (fr) 1998-05-27 1999-05-27 Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
PCT/FR1999/001247 WO1999061661A1 (fr) 1998-05-27 1999-05-27 Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR9806866A FR2779154B1 (fr) 1998-05-27 1998-05-27 Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre

Publications (2)

Publication Number Publication Date
FR2779154A1 FR2779154A1 (fr) 1999-12-03
FR2779154B1 true FR2779154B1 (fr) 2002-07-12

Family

ID=9526907

Family Applications (1)

Application Number Title Priority Date Filing Date
FR9806866A Expired - Fee Related FR2779154B1 (fr) 1998-05-27 1998-05-27 Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre

Country Status (5)

Country Link
EP (1) EP1082461A1 (fr)
JP (1) JP2002516666A (fr)
AU (1) AU3935599A (fr)
FR (1) FR2779154B1 (fr)
WO (1) WO1999061661A1 (fr)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4557347B2 (ja) * 2000-01-30 2010-10-06 株式会社カイノス 特定の塩基配列を有する標的核酸の検出またはその核酸量の測定方法
AU2001234048A1 (en) * 2000-02-22 2001-09-03 Agricultural Research Organization Newe Ya'ar Research Center A method that compares genomic sequences
DE10012540B4 (de) * 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
ATE456668T1 (de) * 2000-04-03 2010-02-15 Cytyc Corp Nachweis und typisierung des papillomavirus mittels pna-sonden
US6248567B1 (en) * 2000-04-24 2001-06-19 Wisconsin Alumni Research Foundation Template-specific termination in a polymerase chain reaction
US6391592B1 (en) * 2000-12-14 2002-05-21 Affymetrix, Inc. Blocker-aided target amplification of nucleic acids
EP1253205A1 (fr) * 2001-04-24 2002-10-30 LION Bioscience AG Procédé de blocage d'amplification d'une séquence sélectionnée
JP2008500002A (ja) * 2003-06-06 2008-01-10 ジーン ロジック インコーポレイテッド 遺伝子発現分析を増強するための方法
GB0703997D0 (en) 2007-03-01 2007-04-11 Oxitec Ltd Methods for detecting nucleic sequences
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
KR101550489B1 (ko) 2010-03-08 2015-09-07 다나-파버 캔서 인스티튜트 인크. 기준 차단 서열을 이용한 완전 cold-pcr 풍부화
US20120225421A1 (en) * 2011-02-28 2012-09-06 Transgenomic, Inc. Kit and method for sequencing a target dna in a mixed population
CA2830361C (fr) 2011-03-31 2021-03-16 Dana-Farber Cancer Institute, Inc. Procedes et compositions pour permettre une cold-pcr multiplexe
ES2686043T3 (es) 2011-09-16 2018-10-16 Lexogen Gmbh Método para preparar una biblioteca de moléculas de ácido nucleico
EP2570487A1 (fr) 2011-09-16 2013-03-20 Lexogen GmbH Procédé de transcription d'acide nucléique
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
CN105506082B (zh) * 2015-12-24 2019-04-09 杭州师范大学附属医院 利用链转移型引物扩增核酸及融合探针的方法
EP3551756A4 (fr) 2016-12-12 2020-07-15 Dana Farber Cancer Institute, Inc. Compositions et procédés pour le codage par code-barres moléculaire de molécules d'adn avant l'enrichissement des mutations et/ou la détection des mutations
WO2019023243A1 (fr) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. Procédés et compositions pour sélectionner et amplifier des cibles d'adn dans un mélange de réaction unique
WO2019079656A1 (fr) * 2017-10-18 2019-04-25 Day Zero Diagnostics, Inc. Enrichissement sélectif d'une population d'adn dans un échantillon d'adn mélangé par suppression ciblée de l'amplification d'adn

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5573907A (en) * 1990-01-26 1996-11-12 Abbott Laboratories Detecting and amplifying target nucleic acids using exonucleolytic activity
US6180338B1 (en) * 1992-08-04 2001-01-30 Beckman Coulter, Inc. Method, reagent and kit for the detection and amplification of nucleic acid sequences
GB2293238A (en) * 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
EP0832280A2 (fr) * 1995-06-07 1998-04-01 Abbott Laboratories Procede de masquage de sonde servant a limiter le signal d'arriere-plan dans une reaction d'amplification
WO1997007235A2 (fr) * 1995-08-14 1997-02-27 Abbott Laboratories Methode integrale d'amplification d'acide nucleique
US6482590B1 (en) * 1996-12-20 2002-11-19 Aventis Behring Gmbh Method for polynucleotide amplification

Also Published As

Publication number Publication date
FR2779154A1 (fr) 1999-12-03
AU3935599A (en) 1999-12-13
WO1999061661A1 (fr) 1999-12-02
JP2002516666A (ja) 2002-06-11
EP1082461A1 (fr) 2001-03-14

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Legal Events

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ST Notification of lapse

Effective date: 20060131