FR2646440A1 - Isolation medium for the identification of Salmonella bacteria - Google Patents
Isolation medium for the identification of Salmonella bacteria Download PDFInfo
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- FR2646440A1 FR2646440A1 FR8905594A FR8905594A FR2646440A1 FR 2646440 A1 FR2646440 A1 FR 2646440A1 FR 8905594 A FR8905594 A FR 8905594A FR 8905594 A FR8905594 A FR 8905594A FR 2646440 A1 FR2646440 A1 FR 2646440A1
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- salmonella
- isolation medium
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- 241000607142 Salmonella Species 0.000 title claims abstract description 33
- 238000002955 isolation Methods 0.000 title claims abstract description 19
- 229920005862 polyol Polymers 0.000 claims abstract description 9
- 150000003077 polyols Chemical class 0.000 claims abstract description 9
- 239000007793 ph indicator Substances 0.000 claims abstract description 8
- 230000020477 pH reduction Effects 0.000 claims abstract description 5
- 239000001888 Peptone Substances 0.000 claims abstract description 4
- 108010080698 Peptones Proteins 0.000 claims abstract description 4
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical class CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims abstract description 4
- 235000019319 peptone Nutrition 0.000 claims abstract description 4
- 229940066779 peptones Drugs 0.000 claims abstract description 4
- 229920001817 Agar Polymers 0.000 claims description 6
- 239000008272 agar Substances 0.000 claims description 6
- 108010005774 beta-Galactosidase Proteins 0.000 claims description 5
- 238000006243 chemical reaction Methods 0.000 claims description 4
- PGSADBUBUOPOJS-UHFFFAOYSA-N neutral red Chemical group Cl.C1=C(C)C(N)=CC2=NC3=CC(N(C)C)=CC=C3N=C21 PGSADBUBUOPOJS-UHFFFAOYSA-N 0.000 claims description 4
- 102000005936 beta-Galactosidase Human genes 0.000 claims description 3
- 150000002009 diols Chemical class 0.000 claims description 3
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 claims description 3
- 238000000034 method Methods 0.000 claims description 3
- ULWHHBHJGPPBCO-UHFFFAOYSA-N propane-1,1-diol Chemical class CCC(O)O ULWHHBHJGPPBCO-UHFFFAOYSA-N 0.000 claims description 3
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 2
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical class CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 claims description 2
- 125000004432 carbon atom Chemical group C* 0.000 claims description 2
- 239000003593 chromogenic compound Substances 0.000 claims description 2
- 239000002609 medium Substances 0.000 abstract description 20
- 239000001963 growth medium Substances 0.000 abstract description 2
- 238000001514 detection method Methods 0.000 description 9
- 241000305071 Enterobacterales Species 0.000 description 6
- 241000588769 Proteus <enterobacteria> Species 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 5
- 241000588724 Escherichia coli Species 0.000 description 4
- -1 bromo chloro indoxyl galactoside Chemical class 0.000 description 4
- 230000004069 differentiation Effects 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 241000894007 species Species 0.000 description 3
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 description 2
- 241000588923 Citrobacter Species 0.000 description 2
- PCKPVGOLPKLUHR-UHFFFAOYSA-N OH-Indolxyl Natural products C1=CC=C2C(O)=CNC2=C1 PCKPVGOLPKLUHR-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-FPRJBGLDSA-N beta-D-galactose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-FPRJBGLDSA-N 0.000 description 2
- 229940009976 deoxycholate Drugs 0.000 description 2
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- DNIAPMSPPWPWGF-UHFFFAOYSA-N monopropylene glycol Natural products CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 229960004063 propylene glycol Drugs 0.000 description 2
- 235000013772 propylene glycol Nutrition 0.000 description 2
- OPIFSICVWOWJMJ-AEOCFKNESA-N 5-bromo-4-chloro-3-indolyl beta-D-galactoside Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1OC1=CNC2=CC=C(Br)C(Cl)=C12 OPIFSICVWOWJMJ-AEOCFKNESA-N 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000219470 Mirabilis Species 0.000 description 1
- 241000588770 Proteus mirabilis Species 0.000 description 1
- 241000204914 Salmonella enterica subsp. enterica serovar Give Species 0.000 description 1
- 235000005811 Viola adunca Nutrition 0.000 description 1
- 240000009038 Viola odorata Species 0.000 description 1
- 235000013487 Viola odorata Nutrition 0.000 description 1
- 235000002254 Viola papilionacea Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 230000001925 catabolic effect Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 235000013601 eggs Nutrition 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000000411 inducer Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
- C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
- C12Q1/10—Enterobacteria
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
- G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
- G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
- G01N2333/255—Salmonella (G)
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Toxicology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
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- General Engineering & Computer Science (AREA)
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- Genetics & Genomics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
La présente invention se rapporte à un milieu d'isolement permettant l'identification des bactéries de l'espèce Salmonella. The present invention relates to an isolation medium allowing the identification of bacteria of the Salmonella species.
L'identification de la bactérie Salmonella pathogène pour l'homme est un problème majeur de la bactériologie médicale et de la surveillance de l'hygiène agro-alimentaire. The identification of the pathogenic Salmonella bacteria for humans is a major problem in medical bacteriology and the monitoring of food hygiene.
Ainsi, dans le cas d'épidémies transmises par des élevages de poules, les volailles infestées au niveau du tractus intestinal ne sont pas malades mais constituent un réservoir de Salmonella. Celles-ci peuvent être propagées, notamment par les oeufs, dans l'alimentation à la suite de ces épidémies. D'ailleurs, la Salmonella est une bactérie à déclaration obligatoire. Thus, in the case of epidemics transmitted by chicken farms, infested poultry in the intestinal tract are not sick but constitute a reservoir of Salmonella. These can be spread, especially by eggs, in food following these epidemics. In fact, Salmonella is a reportable bacteria.
Aujourd'hui, il devient nécessaire de prévoir la mise en oeuvre à grande échelle de la détection des sites infectés et plus particulièrement des fermes infectées par la bactérie Salmonella pour réduire ces épidémies. Today, it becomes necessary to provide for the large-scale implementation of the detection of infected sites and more particularly of farms infected with the Salmonella bacteria to reduce these epidemics.
En fait, les Salmonella sont généralement à reconnaître parmi les espèces commensales, Escherichia Çoli et Proteus. In fact, Salmonella are generally to be recognized among the commensal species, Escherichia Çoli and Proteus.
La détection de Salmonella est habituellement réalisée sur un milieu gélosé d'isolement sélectif pour entérobactéries permettant la différenciation des entérobactéries pathogènes et la détection des colonies suspectes Salmonella. Un milieu d'isolement idéal doit permettre la croissance d'entérobactéries, la différenciation des diverses espèces présentes afin de permettre l'identification ultérieure d'une colonie de chaque type, et la détection des colonies suspectes Salmonella. The detection of Salmonella is usually carried out on an agar medium selective isolation for enterobacteria allowing the differentiation of pathogenic enterobacteria and the detection of suspicious Salmonella colonies. An ideal isolation medium should allow the growth of enterobacteria, the differentiation of the various species present in order to allow the subsequent identification of a colony of each type, and the detection of suspicious Salmonella colonies.
Or, les milieux d'isolement d'entérobactéries de l'art antérieur, ne répondent que partiellement à la détection de Salmonella. En effet, on rencontre des bactéries commensales fréquentes, de type Proteus mirabilis qui se révèlent des faux positifs sur ces milieux. However, the enterobacterial isolation media of the prior art only partially respond to the detection of Salmonella. In fact, frequent commensal bacteria are encountered, of the Proteus mirabilis type, which prove to be false positives in these environments.
Parmi les milieux sélectifs gélosés proposés, le milieu Hektoen est généralement préféré, tout en étant l'un des plus chers. Among the selective agar media offered, the Hektoen medium is generally preferred, while being one of the most expensive.
Ainsi, sur ce milieu Hektoen, les bactéries Salmonella donnent des colonies bleues à centre noir (lac sac sal~H2S+), et la bactérie mirabilis qui a les mêmes propriétés donne également des colonies bleues à centre noir. Ces faux positifs obligent donc à un surcroît de travail et de dépenses car ils rendent obligatoire l'examen ultérieur de plusieurs colonies suspectes au lieu d'une, éventuellement sans résultat positif, sans parler du risque d'erreur de diagnostic après une lecture rapide. Thus, on this Hektoen medium, the Salmonella bacteria give blue colonies with a black center (lac sac sal ~ H2S +), and the mirabilis bacterium which has the same properties also gives blue colonies with a black center. These false positives therefore require additional work and expenditure because they make the subsequent examination of several suspicious colonies mandatory instead of one, possibly without a positive result, not to mention the risk of diagnostic error after a rapid reading.
De plus, l'ambigulté de la réponse Salmonella requiert aussi pour le passage à l'étape ultérieure de réisolement de colonies et de différenciation biochimique, une plus grande qualification que pour une simple reconnaissance d'une coloration de colonies. La différenciation biochimique peut enfin retarder llidentification et donc imposer un délai supplémentaire. In addition, the ambiguity of the Salmonella response also requires a higher qualification for the passage to the later stage of colony re-isolation and biochemical differentiation than for a simple recognition of colony staining. Biochemical differentiation can finally delay identification and therefore impose an additional delay.
L'objet de la présente invention est précisément de remédier à ces inconvénients et de proposer un milieu d'isolement permettant la détection de colonies de Salmonella, de manière non ambiguë par une coloration spécifique de la colonie. La présente invention n'utilise en effet pas les caractères classiques ff2S+lac sac sal de repérages de Salmonella qui laissent apparaître comme faux positifs les Proteus. The object of the present invention is precisely to remedy these drawbacks and to propose an isolation medium allowing the detection of colonies of Salmonella, in an unambiguous manner by a specific coloration of the colony. The present invention does not in fact use the conventional characters ff2S + lac sac sal for Salmonella scouting which allow Proteus to appear as false positives.
Plus précisément, la présente invention se rapporte à un milieu d'isolement pour l'identification de la - bactérie Salmonella caractérisé en ce qu'à un support de culture contenant des peptones, on ajoute un polyol métabolisable par Salmonella et un indicateur de pH réagissant à l'acidification. More specifically, the present invention relates to an isolation medium for the identification of the - Salmonella bacteria, characterized in that to a culture support containing peptones, a polyol metabolizable by Salmonella and a reactive pH indicator are added. acidification.
Parmi les polyols utilisables, il faut citer plus particulièrement les diols comprenant 2 à 10 atomes de carbone tels que l'éthanediol, les propanediols et les butanediols, notamment le 1,2 propanediol. Ces diols sont en effet métabolisés par la bactérie Salmonella pour donner des espèces acides capables de faire réagir des indicateurs de pH tels que le rouge neutre. Au contraire, les espèces commensales telles que E. coli et
Proteus ne donnent pas ce type de réaction. Among the polyols which can be used, mention should be made more particularly of diols comprising 2 to 10 carbon atoms such as ethanediol, propanediols and butanediols, in particular 1,2 propanediol. These diols are in fact metabolized by the Salmonella bacteria to give acidic species capable of reacting pH indicators such as neutral red. On the contrary, commensal species such as E. coli and
Proteus does not give this type of reaction.
Ce milieu est plus particulièrement utilisable pour la détection de Salmonella alimentaires qui donnent toutes des résultats positifs sur ce milieu. Pour la mise en oeuvre du procédé, on préfère utiliser des milieux de culture gélosés appropriés pour la culture en colonies qui permet d'identifier plus rapidement les colonies de Salmonella. This medium is more particularly usable for the detection of dietary Salmonella which all give positive results on this medium. For the implementation of the method, it is preferable to use agar culture media suitable for culture in colonies which makes it possible to identify Salmonella colonies more quickly.
Parmi les milieux favorisant le développement des entérobactéries par exemple, on pourra utiliser un milieu contenant du désoxycholate. Si l'on souhaite augmenter la précision de la détection, on peut ajouter au milieu en cause un substrat de la béta-galactosidase notamment un substrat chromogène de celle-ci type bromo chloro indoxyl galactoside, bromo naphtyl galactoside ou hydroxyquinoline galactoside et, éventuellement en présence d'un inducteur tel que l'IPTG. En effet la souche de Salmonella étant béta gai; elle ne réagit pas sur ce substrat. Among the media favoring the development of enterobacteria for example, one could use a medium containing deoxycholate. If it is desired to increase the accuracy of the detection, a substrate of beta-galactosidase may be added to the medium in question, in particular a chromogenic substrate thereof, such as bromo chloro indoxyl galactoside, bromo naphthyl galactoside or hydroxyquinoline galactoside and, optionally presence of an inducer such as IPTG. Indeed the strain of Salmonella being beta gay; it does not react on this substrate.
L'intérêt des polyols selon l'invention, lorsque l'on utilise les deux caractères d'identification précédents, est que la présence de polyols ne masque pas le caractère béta gal+ à la différence de la plupart des hydrates de carbone qui, par répression catabolique, peuvent masquer le caractère béta gal+. Lorsque ces deux caractères d'identification sont visualisés par des réactions colorées, on peut identifier par un choix judicieux les différents types de bactéries particulièrement Salmonella,
Proteus et E. coli. Ainsi, le rouge neutre comme indicateur de pH et l'X-gal comme substrat de la béta-galactosidase permettent de distinguer
Salmonella de E. coli, Citrobacter et Proteus dans un mélange les contenant comme cela apparaîtra à la lecture de l'exemple ci-dessous.The advantage of the polyols according to the invention, when the two preceding identification characters are used, is that the presence of polyols does not mask the beta gal + character unlike most carbohydrates which, by repression catabolic, can mask the beta gal + character. When these two identification characters are visualized by colored reactions, the different types of bacteria, particularly Salmonella, can be identified by judicious choice.
Proteus and E. coli. Thus, neutral red as a pH indicator and X-gal as a substrate for beta-galactosidase make it possible to distinguish
Salmonella from E. coli, Citrobacter and Proteus in a mixture containing them as will appear on reading the example below.
Il est bien entendu possible dans ce type de milieu d'utiliser d'autres caractères négatifs de Salmonella par exemple béta glu, dans ce cas, on utilisera un substrat de la béta-glucosidase. It is of course possible in this type of medium to use other negative Salmonella characters, for example beta glu, in this case, a beta-glucosidase substrate will be used.
De même, il est possible plutôt que de mettre en oeuvre un milieu gélosé, notamment des plaques, d'utiliser d'autres supports tel que support papier, colonne et même de détecter dans le milieu lui-même. Similarly, it is possible rather than using an agar medium, in particular plates, to use other supports such as paper support, column and even to detect in the medium itself.
La présente invention concerne également un procédé permettant l'identification de Salmonella dans lequel on cultive l'échantillon sur un milieu selon l'invention et on détecte la présence de
Salmonella par la réaction de l'indicateur de pH à l'acidification du milieu.The present invention also relates to a method allowing the identification of Salmonella in which the sample is cultured on a medium according to the invention and the presence of
Salmonella by the reaction of the pH indicator to the acidification of the medium.
L'exemple donné ci-dessous à titre non limitatif permettra de mettre en évidence d'autres avantages et caractéristiques de la présente invention. The example given below without implied limitation will make it possible to highlight other advantages and characteristics of the present invention.
EXEMPLE :
Milieu d'isolement permettant la détection des Salmonella
On réalise un milieu ayant la composition suivante:
Constituants g/litre d'eau . 1,2 propane-diol 5 . Peptones 5 Extrait de levure 2 . Désoxycholate 1 . Bromo Chloro Indoxyl Galactoside 0,1 . Rouge neutre 0,03 Agar 15
Par ensemencement de ce milieu avec différents types d'entérobactéries et après 48 heures de culture, on obtient les résultats suivants : les Salmonella donnent des colonies rouges, les E. coli des colonies bleu-vert, les Citrobacter des colonies bleu-violet et les Proteus des colonies incolores. EXAMPLE:
Isolation medium allowing the detection of Salmonella
A medium is produced having the following composition:
Constituents g / liter of water. 1,2 propane-diol 5. Peptones 5 Yeast extract 2. Deoxycholate 1. Bromo Chloro Indoxyl Galactoside 0.1. Neutral red 0.03 Agar 15
By seeding this medium with different types of enterobacteria and after 48 hours of culture, the following results are obtained: the Salmonella give red colonies, the E. coli of the blue-green colonies, the Citrobacter of the blue-violet colonies and the Proteus of colorless colonies.
Claims (9)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8905594A FR2646440A1 (en) | 1989-04-27 | 1989-04-27 | Isolation medium for the identification of Salmonella bacteria |
| FR8909114A FR2649410B2 (en) | 1989-04-27 | 1989-07-06 | IMPROVEMENT OF AN ISOLATION MEDIUM FOR THE IDENTIFICATION OF THE BACTERIA SALMONELLA |
| US07/510,983 US5098832A (en) | 1989-04-27 | 1990-04-19 | Isolating medium for identifying the salmonella bacterium |
| JP2108966A JP2995632B2 (en) | 1989-04-27 | 1990-04-26 | Separation media for Salmonella identification |
| DE69010268T DE69010268T2 (en) | 1989-04-27 | 1990-04-27 | Isolation medium for the identification of Salmonella bacteria. |
| ES90401158T ES2056401T3 (en) | 1989-04-27 | 1990-04-27 | IMPROVEMENT OF AN INSULATION MEDIA FOR THE IDENTIFICATION OF THE BACTERIA SALMONELLA. |
| AT90401158T ATE107963T1 (en) | 1989-04-27 | 1990-04-27 | ISOLATION MEDIUM FOR IDENTIFICATION OF SALMONELLA BACTERIA. |
| DK90401158.2T DK0395532T3 (en) | 1989-04-27 | 1990-04-27 | Isolation medium for identification of Salmonella bacteria |
| EP90401158A EP0395532B1 (en) | 1989-04-27 | 1990-04-27 | Isolation medium for the identification of the bacteria Salmonella |
| US07/819,478 US5194374A (en) | 1989-04-27 | 1992-01-10 | Isolating medium for identifying the salmonella bacterium |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR8905594A FR2646440A1 (en) | 1989-04-27 | 1989-04-27 | Isolation medium for the identification of Salmonella bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2646440A1 true FR2646440A1 (en) | 1990-11-02 |
| FR2646440B1 FR2646440B1 (en) | 1994-02-18 |
Family
ID=9381191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR8905594A Granted FR2646440A1 (en) | 1989-04-27 | 1989-04-27 | Isolation medium for the identification of Salmonella bacteria |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2646440A1 (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2050418A (en) * | 1979-05-25 | 1981-01-07 | Api Labor | Detecting salmonella and serratia and distinguishing them from proteus and providencia |
| EP0163867A1 (en) * | 1984-05-09 | 1985-12-11 | TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION | Medium for beta-galactosidase test |
| EP0206277A1 (en) * | 1985-06-28 | 1986-12-30 | TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION | Medium composition for the identification of microorganisms |
-
1989
- 1989-04-27 FR FR8905594A patent/FR2646440A1/en active Granted
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2050418A (en) * | 1979-05-25 | 1981-01-07 | Api Labor | Detecting salmonella and serratia and distinguishing them from proteus and providencia |
| EP0163867A1 (en) * | 1984-05-09 | 1985-12-11 | TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION | Medium for beta-galactosidase test |
| EP0206277A1 (en) * | 1985-06-28 | 1986-12-30 | TERUMO KABUSHIKI KAISHA trading as TERUMO CORPORATION | Medium composition for the identification of microorganisms |
Non-Patent Citations (3)
| Title |
|---|
| APPLIED & ENVIRONMENTAL MICROBIOLOGY, vol. 34, no. 5, novembre 1977, pages 595-596, American Society for Microbiology, US; J.Y. D'AOUST: "Limitations of lysine-iron-cystine-neutral red broth in the presumptive identification of Salmonella" * |
| BIOCHEM. J., vol. 231, 1985, pages 145-149, GB; J. ROS et al.: "Propanediol oxidoreductases of Escherichia coli, Klebsiella pneumoniae and Salmonella typhimurium" * |
| BIOLOGICAL ABSTRACTS, vol. 80, no. 8, 1985, page 774, résumé no. 71128, Philadelphia, PA, US; Y. ICHIKAWA et al.: "Purification and properties of propanediol dehydratase from Propionibacterium freudenreichii", & J. FERMENT TECHNOL. 63(2): 135-142. 1985 * |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2646440B1 (en) | 1994-02-18 |
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