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FR2452517A1 - NOVEL ESSENTIALLY PURE PLASMID AND PROCESS FOR PRODUCING THE SAME - Google Patents

NOVEL ESSENTIALLY PURE PLASMID AND PROCESS FOR PRODUCING THE SAME

Info

Publication number
FR2452517A1
FR2452517A1 FR8006946A FR8006946A FR2452517A1 FR 2452517 A1 FR2452517 A1 FR 2452517A1 FR 8006946 A FR8006946 A FR 8006946A FR 8006946 A FR8006946 A FR 8006946A FR 2452517 A1 FR2452517 A1 FR 2452517A1
Authority
FR
France
Prior art keywords
plasmid
puc2
essentially pure
producing
same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
FR8006946A
Other languages
French (fr)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pharmacia and Upjohn Co
Original Assignee
Upjohn Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Upjohn Co filed Critical Upjohn Co
Publication of FR2452517A1 publication Critical patent/FR2452517A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/54—Streptomyces fradiae

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

L'INVENTION CONCERNE UN COMPOSE CHIMIQUE NOUVEAU, QUI EST LE PLASMIDE PUC2 ESSENTIELLEMENT PUR. LE PLASMIDE PUC2 PEUT ETRE OBTENU A PARTIR D'UNE CULTURE BIOLOGIQUEMENT PURE DU MICRO-ORGANISME STREPTOMYCES FRADIAE NRRL11444. LE PLASMIDE PUC2 EST UTILE COMME VEHICULE DE CLONAGE DANS DES TRAVAUX DE RECOMBINAISON DE L'ADN. PAR EXEMPLE, EN UTILISANT LA METHODOLOGIE DE L'ADN, ON PEUT INSERER UN GENE DESIRE, PAR EXEMPLE LE GENE DE L'INSULINE, DANS LE PLASMIDE PUC2 ET ON PEUT ENSUITE TRANSFORMER LE PLASMIDE RESULTANT EN UN MICRO-ORGANISME-HOTE CONVENABLE QUI, PAR CULTURE, PRODUIT L'INSULINE DESIREE.THE INVENTION CONCERNS A NEW CHEMICAL COMPOUND, WHICH IS ESSENTIALLY PURE PUC2 PLASMID. PUC2 PLASMID CAN BE OBTAINED FROM A BIOLOGICALLY PURE CULTURE OF MICRO-ORGANISM STREPTOMYCES FRADIAE NRRL11444. THE PUC2 PLASMID IS USEFUL AS A CLONING VEHICLE IN DNA RECOMBINATION WORK. FOR EXAMPLE, USING THE DNA METHODOLOGY, A DESIRED GENE, FOR EXAMPLE THE GENE OF INSULIN, CAN BE INSERTED INTO THE PUC2 PLASMID AND THE RESULTING PLASMID CAN THEN BE TRANSFORMED INTO A SUITABLE HOST MICRO-ORGANISM WHICH, BY CULTURE, PRODUCES THE DESIRED INSULIN.

FR8006946A 1979-03-29 1980-03-28 NOVEL ESSENTIALLY PURE PLASMID AND PROCESS FOR PRODUCING THE SAME Pending FR2452517A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US2488879A 1979-03-29 1979-03-29

Publications (1)

Publication Number Publication Date
FR2452517A1 true FR2452517A1 (en) 1980-10-24

Family

ID=21822888

Family Applications (1)

Application Number Title Priority Date Filing Date
FR8006946A Pending FR2452517A1 (en) 1979-03-29 1980-03-28 NOVEL ESSENTIALLY PURE PLASMID AND PROCESS FOR PRODUCING THE SAME

Country Status (6)

Country Link
JP (1) JPS55133397A (en)
DE (1) DE3008645A1 (en)
FR (1) FR2452517A1 (en)
GB (1) GB2045252A (en)
IT (1) IT8020655A0 (en)
NL (1) NL8001379A (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2494718A1 (en) * 1980-11-21 1982-05-28 Upjohn Co

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS57206699A (en) * 1981-06-10 1982-12-18 Takeda Chem Ind Ltd Plasmid patm 3 and its preparation
US4703009A (en) * 1983-03-08 1987-10-27 Merck & Co., Inc. RDNA cloning vector pVE1, deletion and hybrid mutants and recombinant derivatives thereof products and processes

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2494718A1 (en) * 1980-11-21 1982-05-28 Upjohn Co

Also Published As

Publication number Publication date
GB2045252A (en) 1980-10-29
JPS55133397A (en) 1980-10-17
IT8020655A0 (en) 1980-03-14
NL8001379A (en) 1980-10-01
DE3008645A1 (en) 1980-10-09

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