FR2446111A1 - Soluble hydrophobic antigen complexes and vaccines - prepd. by solubilisation of microorganisms with sodium desoxy:cholate, lysozyme and ethylene-di:amine-tetra:acetate - Google Patents
Soluble hydrophobic antigen complexes and vaccines - prepd. by solubilisation of microorganisms with sodium desoxy:cholate, lysozyme and ethylene-di:amine-tetra:acetateInfo
- Publication number
- FR2446111A1 FR2446111A1 FR7901301A FR7901301A FR2446111A1 FR 2446111 A1 FR2446111 A1 FR 2446111A1 FR 7901301 A FR7901301 A FR 7901301A FR 7901301 A FR7901301 A FR 7901301A FR 2446111 A1 FR2446111 A1 FR 2446111A1
- Authority
- FR
- France
- Prior art keywords
- sodium
- vaccines
- complexes
- prod
- solubilisation
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000427 antigen Substances 0.000 title abstract 4
- 102000036639 antigens Human genes 0.000 title abstract 4
- 108091007433 antigens Proteins 0.000 title abstract 4
- 230000002209 hydrophobic effect Effects 0.000 title abstract 4
- 229910052708 sodium Inorganic materials 0.000 title abstract 3
- 239000011734 sodium Substances 0.000 title abstract 3
- 229960005486 vaccine Drugs 0.000 title abstract 3
- 102000016943 Muramidase Human genes 0.000 title abstract 2
- 108010014251 Muramidase Proteins 0.000 title abstract 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 title abstract 2
- 239000004325 lysozyme Substances 0.000 title abstract 2
- 229960000274 lysozyme Drugs 0.000 title abstract 2
- 235000010335 lysozyme Nutrition 0.000 title abstract 2
- 244000005700 microbiome Species 0.000 title abstract 2
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 title 1
- 229940099352 cholate Drugs 0.000 title 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 title 1
- 230000000890 antigenic effect Effects 0.000 abstract 4
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 abstract 2
- 102000004169 proteins and genes Human genes 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- FHHPUSMSKHSNKW-SMOYURAASA-M sodium deoxycholate Chemical compound [Na+].C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC([O-])=O)C)[C@@]2(C)[C@@H](O)C1 FHHPUSMSKHSNKW-SMOYURAASA-M 0.000 abstract 2
- 108091005804 Peptidases Proteins 0.000 abstract 1
- 239000004365 Protease Substances 0.000 abstract 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 230000015572 biosynthetic process Effects 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- 239000000356 contaminant Substances 0.000 abstract 1
- 230000003993 interaction Effects 0.000 abstract 1
- 230000007935 neutral effect Effects 0.000 abstract 1
- 230000000241 respiratory effect Effects 0.000 abstract 1
- 239000012134 supernatant fraction Substances 0.000 abstract 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/02—Bacterial antigens
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Abstract
Extn. of antigenic complexes comprises solubilising the walls of microorganisms (previously purified) by the simultaneous action of sodium desocycholate (0.5-5 mg per mg of proteins), lysozyme (1 mg/ml) and sodium ethylene-diaminotetracetate (25 mmoles). Pref. the solubilisation is effected at neutral pH and 20 degrees C for 12-24 hrs.; the extract is then centrifuged and the soluble supernatant fraction is used directly as antigen source without removal of the solubilising agent. Prod. obtd. comprises a pseudomicellar reassociation (formed by hydrophobic interaction) of hydrophobic proteins, lepopolysaccharides and peptidoglycons in the presence of sodium desoxycholate to give inter- and intra-specific antigenic complexes in which the antigenic sites are not denaturised. The sodium desoxycholate remaining in the prod. aids formation of the soluble, hydrophobic antigenic complexes and helps protect them against protease contaminants. Used for prepn. of vaccines e.g. upper respiratory and bronchial tract vaccines. The process allows large scale prepn. of antigen complexes using large scale homogenisers, centrifuges etc. The prod. is a new hybrid complex prepd. from known strains which preserves the structural integrity qf the antigen molecule and allows more rapid recognition by the specialised cells of the organism.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7901301A FR2446111A1 (en) | 1979-01-12 | 1979-01-12 | Soluble hydrophobic antigen complexes and vaccines - prepd. by solubilisation of microorganisms with sodium desoxy:cholate, lysozyme and ethylene-di:amine-tetra:acetate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| FR7901301A FR2446111A1 (en) | 1979-01-12 | 1979-01-12 | Soluble hydrophobic antigen complexes and vaccines - prepd. by solubilisation of microorganisms with sodium desoxy:cholate, lysozyme and ethylene-di:amine-tetra:acetate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| FR2446111A1 true FR2446111A1 (en) | 1980-08-08 |
| FR2446111B1 FR2446111B1 (en) | 1982-07-02 |
Family
ID=9220943
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| FR7901301A Granted FR2446111A1 (en) | 1979-01-12 | 1979-01-12 | Soluble hydrophobic antigen complexes and vaccines - prepd. by solubilisation of microorganisms with sodium desoxy:cholate, lysozyme and ethylene-di:amine-tetra:acetate |
Country Status (1)
| Country | Link |
|---|---|
| FR (1) | FR2446111A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0182401A1 (en) * | 1984-10-19 | 1986-05-28 | De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezondheid En Cultuur | Vaccines comprising immunogenic protein or peptide complexes |
| WO1995015768A1 (en) * | 1993-12-09 | 1995-06-15 | Heinrich Exner | Adjuvant for antigens, process for producing the same and its use |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE633375A (en) * |
-
1979
- 1979-01-12 FR FR7901301A patent/FR2446111A1/en active Granted
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE633375A (en) * |
Non-Patent Citations (1)
| Title |
|---|
| CA1972 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0182401A1 (en) * | 1984-10-19 | 1986-05-28 | De Staat Der Nederlanden Vertegenwoordigd Door De Minister Van Welzijn, Volksgezondheid En Cultuur | Vaccines comprising immunogenic protein or peptide complexes |
| WO1995015768A1 (en) * | 1993-12-09 | 1995-06-15 | Heinrich Exner | Adjuvant for antigens, process for producing the same and its use |
| DE4499552C1 (en) * | 1993-12-09 | 1997-07-17 | Heinrich Dr Exner | New incomplete and complete adjuvants for antigens |
| US5904925A (en) * | 1993-12-09 | 1999-05-18 | Exner; Heinrich | Adjuvant for antigens, and process for making |
Also Published As
| Publication number | Publication date |
|---|---|
| FR2446111B1 (en) | 1982-07-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ST | Notification of lapse |