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FR2424535A1 - Quantitative determn. of protein in antigen sample - by immuno-electrophoresis and immunodiffusion - Google Patents

Quantitative determn. of protein in antigen sample - by immuno-electrophoresis and immunodiffusion

Info

Publication number
FR2424535A1
FR2424535A1 FR7812093A FR7812093A FR2424535A1 FR 2424535 A1 FR2424535 A1 FR 2424535A1 FR 7812093 A FR7812093 A FR 7812093A FR 7812093 A FR7812093 A FR 7812093A FR 2424535 A1 FR2424535 A1 FR 2424535A1
Authority
FR
France
Prior art keywords
zone
protein
antibody
antigen
intensity
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7812093A
Other languages
French (fr)
Other versions
FR2424535B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
ALADJEM FREDERICK
Original Assignee
ALADJEM FREDERICK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by ALADJEM FREDERICK filed Critical ALADJEM FREDERICK
Priority to FR7812093A priority Critical patent/FR2424535A1/en
Publication of FR2424535A1 publication Critical patent/FR2424535A1/en
Application granted granted Critical
Publication of FR2424535B1 publication Critical patent/FR2424535B1/fr
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
    • G01N21/59—Transmissivity
    • G01N21/5907—Densitometers
    • G01N21/5911—Densitometers of the scanning type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Pathology (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)

Abstract

Concn. of a protein in an antigen sample is quantitatively measured by immunoelectrophoresis. This includes selective protein migration by electrophoresis in one dimension in a thin layer of supporting medium. The antigen is then subjected to immunodiffusion with an antibody source contg. an antibody specific to the protein. The protein and antibody diffuse into reactive contact in another dimension through an area of the medium layer initially free from antigen and antibody, and they react to form an elongated pptn. zone. The zone is scaned optically under dark field illumination at a selected time after the diffusion starts. There are parallel, spaced scan paths that cross the zone transversely to develop electrical intensity signals (the intensity of scattered light at posns. on the path, partly in the zone and partly out). From the signals an electrical sum signal for the sum of the effective excess of each intensity within the zone over intensities outside the zone. This signal is compared with reference signals produced with controls in a similar procedure. The process facilitate distinction among pptes. formed by different proteins; so that quantitative and reliable results are obtd. Rapid, reliable and economic process is used for the analysis of serum, spinal fluid, tissue extracts etc. used in clinical diagnosis.
FR7812093A 1978-04-24 1978-04-24 Quantitative determn. of protein in antigen sample - by immuno-electrophoresis and immunodiffusion Granted FR2424535A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7812093A FR2424535A1 (en) 1978-04-24 1978-04-24 Quantitative determn. of protein in antigen sample - by immuno-electrophoresis and immunodiffusion

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7812093A FR2424535A1 (en) 1978-04-24 1978-04-24 Quantitative determn. of protein in antigen sample - by immuno-electrophoresis and immunodiffusion

Publications (2)

Publication Number Publication Date
FR2424535A1 true FR2424535A1 (en) 1979-11-23
FR2424535B1 FR2424535B1 (en) 1983-10-07

Family

ID=9207521

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7812093A Granted FR2424535A1 (en) 1978-04-24 1978-04-24 Quantitative determn. of protein in antigen sample - by immuno-electrophoresis and immunodiffusion

Country Status (1)

Country Link
FR (1) FR2424535A1 (en)

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3459948A (en) * 1964-04-30 1969-08-05 Gino Cosci Process for quantitatively measuring the color density of stained strips
US3553067A (en) * 1968-03-25 1971-01-05 Millipore Corp Laminated sheet for evaluating diffusive reactions
US3612886A (en) * 1970-02-04 1971-10-12 Kurt Hannig Process for the quantitative determination of light-absorbing or light-reflecting substances distributed on a carrier
FR2138933A1 (en) * 1971-05-24 1973-01-05 Bausch & Lomb
US3768913A (en) * 1970-11-02 1973-10-30 V Klimecki Method and apparatus for evaluation of the intensity of spots
FR2359414A1 (en) * 1976-07-22 1978-02-17 Chinoin Gyogyszer Es Vegyeszet Colour density measurement of chromatograms - using opto-electric conversion and line-by-line resolution of video signal (HU 28.12.76)

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3459948A (en) * 1964-04-30 1969-08-05 Gino Cosci Process for quantitatively measuring the color density of stained strips
US3553067A (en) * 1968-03-25 1971-01-05 Millipore Corp Laminated sheet for evaluating diffusive reactions
US3612886A (en) * 1970-02-04 1971-10-12 Kurt Hannig Process for the quantitative determination of light-absorbing or light-reflecting substances distributed on a carrier
US3768913A (en) * 1970-11-02 1973-10-30 V Klimecki Method and apparatus for evaluation of the intensity of spots
FR2138933A1 (en) * 1971-05-24 1973-01-05 Bausch & Lomb
FR2359414A1 (en) * 1976-07-22 1978-02-17 Chinoin Gyogyszer Es Vegyeszet Colour density measurement of chromatograms - using opto-electric conversion and line-by-line resolution of video signal (HU 28.12.76)

Also Published As

Publication number Publication date
FR2424535B1 (en) 1983-10-07

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