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FR2305161A1 - Detection of fertile period and pregnancy - with test kit contng. buffer and cpd. hydrolysable to p-nitrophenol etc. - Google Patents

Detection of fertile period and pregnancy - with test kit contng. buffer and cpd. hydrolysable to p-nitrophenol etc.

Info

Publication number
FR2305161A1
FR2305161A1 FR7509925A FR7509925A FR2305161A1 FR 2305161 A1 FR2305161 A1 FR 2305161A1 FR 7509925 A FR7509925 A FR 7509925A FR 7509925 A FR7509925 A FR 7509925A FR 2305161 A1 FR2305161 A1 FR 2305161A1
Authority
FR
France
Prior art keywords
pregnancy
acetyl
beta
buffer
fertile period
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
FR7509925A
Other languages
French (fr)
Other versions
FR2305161B1 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alza Corp
Original Assignee
Alza Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alza Corp filed Critical Alza Corp
Priority to FR7509925A priority Critical patent/FR2305161A1/en
Publication of FR2305161A1 publication Critical patent/FR2305161A1/en
Application granted granted Critical
Publication of FR2305161B1 publication Critical patent/FR2305161B1/fr
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/10—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases p-Nitrophenol derivatives
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

New test kit for determining an increase in N-acetyl-beta-glucosaminidase in female body fluids (i.e., for determining the fertile period or pregnancy) consists of: a bibulous absorbant strip impregnated with a non-toxic buffer (A) (to maintain pH 4-5) and a colour indicator (I). The strip is moistened with saliva, or cervical or vaginal fluid. Any colour that develops is further intensified by the addition of a buffer (B) (to maintain pH 9-11). The presence of a colour indicates ovulation or pregnancy. (I) may be alpha-naphthol-N-acetyl-beta-d-glucoraminide, 1- p-nitrocatechol -N-acetyl-beta-d-glucosaminide, 3,3-bis-(p-hydroxyphenyl)phthalid-N-acetyl-beta-d-glucosaminide, or pref. p-nitrophenyl-N-acetyl-beta-d-glucosaminide (II). Safe, simple, reliable test is very accurate, and readily detects ovulation (the fertile period; for conception or rhythmic contraception). Method also allows the diagnosis of pregnancy to be made as early as 10 days after fertilisation.
FR7509925A 1975-03-28 1975-03-28 Detection of fertile period and pregnancy - with test kit contng. buffer and cpd. hydrolysable to p-nitrophenol etc. Granted FR2305161A1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
FR7509925A FR2305161A1 (en) 1975-03-28 1975-03-28 Detection of fertile period and pregnancy - with test kit contng. buffer and cpd. hydrolysable to p-nitrophenol etc.

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR7509925A FR2305161A1 (en) 1975-03-28 1975-03-28 Detection of fertile period and pregnancy - with test kit contng. buffer and cpd. hydrolysable to p-nitrophenol etc.

Publications (2)

Publication Number Publication Date
FR2305161A1 true FR2305161A1 (en) 1976-10-22
FR2305161B1 FR2305161B1 (en) 1978-07-13

Family

ID=9153295

Family Applications (1)

Application Number Title Priority Date Filing Date
FR7509925A Granted FR2305161A1 (en) 1975-03-28 1975-03-28 Detection of fertile period and pregnancy - with test kit contng. buffer and cpd. hydrolysable to p-nitrophenol etc.

Country Status (1)

Country Link
FR (1) FR2305161A1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6234974B1 (en) 1992-08-21 2001-05-22 Unilever Patent Holdings B.V. Monitoring method

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
CHEMICAL ABSTRACTS, VOLUME 80, NO. 3, 21 JANVIER 1974, COLUMBUS, OHIO, USA, PAGE 145, REFERENCE 11646T ROBINSON H.K. "AUTOMATED ASSAY SYSTEM FOR ENDO- AND EXOHEXOSAMINIDASES: BIOCHEM. SOC. TRANS. 1973, 1 4 960-3) *
CHEMICAL ABSTRACTS, VOLUME 81, NO. 17, 28 OCTOBRE 1974 COLUMBUS, OHIO, USA *
PAGE 187, 1ERE COLONNE ABREGE NO. 101.182P YAMAMOTO KAZUHIKO "N-ACYL SPECIFICITY OF TAKA-N-ACETYL-B-D-GLUCOSAMINIDASE. IV. ESSENTIAL REQUIREMENT OF THE 2-ACETAMIDE CARBONYL GROUP FOR THE ENZYMIC HYDROLYSIS" CITE J. BIOCHEM. TOKYO 1974, 75 4 , 931-3 *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6234974B1 (en) 1992-08-21 2001-05-22 Unilever Patent Holdings B.V. Monitoring method

Also Published As

Publication number Publication date
FR2305161B1 (en) 1978-07-13

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