FI864654A - Menetelmä modifioidun ihmisen t-PA-proteiinin valmistamiseksi - Google Patents

Menetelmä modifioidun ihmisen t-PA-proteiinin valmistamiseksi Download PDF

Info

Publication number: FI864654A
Authority: FI; Finland
Prior art keywords: protein; producing; modified human; glycosylation; sites
Prior art date: 1985-11-18

Application number

FI864654A

Other languages

English (en)

Swedish (sv)

Other versions

FI864654A0 (fi

FI91160B (fi

FI91160C (fi

Inventor

Bhabatosh Chaudhuri

Bernd Meyhack

Jutta Heim

Oostrum Jan Van

Original Assignee

Ciba Geigy Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1985-11-18

Filing date

1986-11-14

Publication date

1987-05-19

1986-11-14 Application filed by Ciba Geigy Ag filed Critical Ciba Geigy Ag

1986-11-14 Publication of FI864654A0 publication Critical patent/FI864654A0/fi

1987-05-19 Publication of FI864654A publication Critical patent/FI864654A/fi

1994-02-15 Publication of FI91160B publication Critical patent/FI91160B/fi

1994-05-25 Application granted granted Critical

1994-05-25 Publication of FI91160C publication Critical patent/FI91160C/fi

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
Biomedical Technology (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
General Engineering & Computer Science (AREA)
Biotechnology (AREA)
Molecular Biology (AREA)
General Health & Medical Sciences (AREA)
Biochemistry (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Biophysics (AREA)
Physics & Mathematics (AREA)
Medicinal Chemistry (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Animal Behavior & Ethology (AREA)
General Chemical & Material Sciences (AREA)
Mycology (AREA)
Pharmacology & Pharmacy (AREA)
Hematology (AREA)
Veterinary Medicine (AREA)
Diabetes (AREA)
Public Health (AREA)
Chemical Kinetics & Catalysis (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Peptides Or Proteins (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Saccharide Compounds (AREA)

FI864654A 1985-11-18 1986-11-14 Menetelmä modifioidun ihmisen t-PA-proteiinin valmistamiseksi FI91160C (fi)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
GB8528321		1985-11-18
GB858528321A GB8528321D0 (en)	1985-11-18	1985-11-18	Modified fibrinolytic agents

Publications (4)

Publication Number	Publication Date
FI864654A0 FI864654A0 (fi)	1986-11-14
FI864654A true FI864654A (fi)	1987-05-19
FI91160B FI91160B (fi)	1994-02-15
FI91160C FI91160C (fi)	1994-05-25

Family

ID=10588363

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
FI864654A FI91160C (fi)	1985-11-18	1986-11-14	Menetelmä modifioidun ihmisen t-PA-proteiinin valmistamiseksi

Country Status (17)

Country	Link
EP (1)	EP0225286B1 (fi)
JP (1)	JPS62272976A (fi)
KR (1)	KR920007666B1 (fi)
AT (1)	ATE78514T1 (fi)
AU (1)	AU598490B2 (fi)
DE (1)	DE3686136T2 (fi)
DK (1)	DK174977B1 (fi)
ES (1)	ES2043607T3 (fi)
FI (1)	FI91160C (fi)
GB (1)	GB8528321D0 (fi)
GR (1)	GR3006041T3 (fi)
IE (1)	IE57866B1 (fi)
IL (1)	IL80659A (fi)
NO (1)	NO175266C (fi)
NZ (1)	NZ218305A (fi)
PT (1)	PT83752B (fi)
ZA (1)	ZA868689B (fi)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ATE151111T1 (de) *	1984-10-01	1997-04-15	Genzyme Corp	Rekombinante dns-verfahren und dadurch hergestellte produkte
ES2031826T3 (es) *	1985-12-23	1993-01-01	Chiron Corporation	Procedimiento para producir nuevos peptidos activadores del plasminogeno.
US5258298A (en) *	1986-01-31	1993-11-02	Genetics Institute, Inc.	Deletion and glycosylation mutant of human tissue plasminogen activator
US5837518A (en) *	1986-01-31	1998-11-17	Genetics Institute, Inc.	Thrombolytic proteins
US5002887A (en) *	1986-01-31	1991-03-26	Genetics Institute, Inc.	Truncated thrombolytic proteins
IE81073B1 (en) *	1986-03-18	2000-01-12	Genentech Inc	Modified human tissue-type plasminogen activator and its preparation
US5589361A (en) *	1986-03-18	1996-12-31	Genentech, Inc.	Human tissue-type plasminogen activator variant
US5047241A (en) *	1986-07-11	1991-09-10	American Home Products Corporation	Tris-Kringle plasminogen activator with novel K2 insert
EP0276846A3 (en) *	1987-01-29	1989-07-26	Zymogenetics, Inc.	Colony-stimulating factor derivatives
JPH084501B2 (ja) *	1987-03-20	1996-01-24	エーザイ株式会社	糖鎖に関する変異ｔＰＡ
US6682733B1 (en) *	1987-06-18	2004-01-27	Roche Diagnostics, Gmbh	Fibrinolytic enzymes
SE8702562L (sv) *	1987-06-18	1988-12-19	Kabigen Ab	Nya fibrinolytiska enzymer
US5266474A (en) *	1987-06-24	1993-11-30	Genentech, Inc.	Balanced inducible transcription system
US5302390A (en) *	1987-07-01	1994-04-12	Beecham Group Plc	Hybrid proteins of human plasminogen and human t-PA, pharmaceutical compositions and methods of treatment
AU2081788A (en) *	1987-07-06	1989-01-30	Genetics Institute Inc.	Novel thrombolytic proteins
AU1893988A (en) *	1987-07-13	1989-01-19	Collaborative Research Inc.	Nonglycosylated plasminogen activator and method of preparation
US5270198A (en) *	1988-05-20	1993-12-14	Genentech, Inc.	DNA molecules encoding variants of tissue plasminogen activators, vectors, and host cells
IL90146A (en) *	1988-05-20	1994-10-07	Genentech Inc	Glycosylation derivatives of tissue plasminogen activator
US5346824A (en) *	1988-05-20	1994-09-13	Genentech, Inc.	DNA encoding variants of tissue plasminogen activators and expression vectors and hosts thereof
US5258180A (en) *	1988-09-02	1993-11-02	Genetech, Inc.	Tissue plasminogen activator having fibrin specific properties and deletion of amino acids 466-970, compositions and methods of treatment
US5108901A (en) *	1988-09-02	1992-04-28	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties
US5262170A (en) *	1988-09-02	1993-11-16	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties and substituted at amino acid positions 296-299, DNA molecules encoding them, vectors, and host cells
US5714145A (en) *	1988-09-02	1998-02-03	Genentech, Inc.	Tissue plasminogen activator having zymogenic or fibrin specific properties
WO1991015235A1 (en) *	1990-03-30	1991-10-17	Survival Technology, Inc.	t-PA ENHANCER PROFILE
US5242688A (en) *	1990-12-24	1993-09-07	Eli Lilly And Company	Method of treating thromboembolic disorders by administration of diglycosylated t-pa variants
PT786257E (pt) *	1992-06-03	2003-12-31	Genentech Inc	Variantes de glicosilacao do activador de plasminogenio tissular com propriedades terapeuticas meloradas
EP0663009A1 (en) *	1993-08-04	1995-07-19	Novartis AG	High molecular weight desulphatohirudin
US6706504B1 (en)	1996-11-12	2004-03-16	The Scripps Research Institute	Tissue type plasminogen activator (t-PA) variants: compositions and methods of use
US20150239929A1 (en) *	2012-06-14	2015-08-27	Portola Pharmaceuticals, Inc.	Method for purification of recombinant factor xa derivatives

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
ZA833174B (en) *	1982-05-05	1984-08-29	Genentech Inc	Human tissue plasminogen activator
IL68561A (en) *	1982-05-05	1991-01-31	Genentech Inc	Preparation of polypeptide with human tissue plasminogen activator function,processes for making it,and dna and transformed cell intermediates thereof
EP0125254A1 (en) *	1982-10-28	1984-11-21	Beecham Group Plc	Enzyme derivatives and their use in the treatment of thrombosis
IE56716B1 (en) *	1983-01-19	1991-11-20	Genentech Inc	Method for producing human tpa and expression vectors therefor
ATE151111T1 (de) *	1984-10-01	1997-04-15	Genzyme Corp	Rekombinante dns-verfahren und dadurch hergestellte produkte
DE3537176C2 (de) *	1984-10-18	1994-06-09	Zymogenetics Inc	Gewebeplasminogenaktivator und Verfahren zu seiner Herstellung
JPH0811066B2 (ja) *	1985-09-30	1996-02-07	インテグレーテッド・ジエネティックス・インコーポレーテッド	組換えｄｎａが生産するヒト子宮プラスミノ−ゲンアクチベ−タ−

1985
- 1985-11-18 GB GB858528321A patent/GB8528321D0/en active Pending
1986
- 1986-11-13 ES ES86810518T patent/ES2043607T3/es not_active Expired - Lifetime
- 1986-11-13 EP EP86810518A patent/EP0225286B1/en not_active Expired - Lifetime
- 1986-11-13 AT AT86810518T patent/ATE78514T1/de not_active IP Right Cessation
- 1986-11-13 DE DE8686810518T patent/DE3686136T2/de not_active Expired - Lifetime
- 1986-11-14 FI FI864654A patent/FI91160C/fi not_active IP Right Cessation
- 1986-11-17 PT PT83752A patent/PT83752B/pt not_active IP Right Cessation
- 1986-11-17 AU AU65200/86A patent/AU598490B2/en not_active Expired
- 1986-11-17 NZ NZ218305A patent/NZ218305A/en unknown
- 1986-11-17 JP JP61272033A patent/JPS62272976A/ja active Pending
- 1986-11-17 DK DK198605491A patent/DK174977B1/da not_active IP Right Cessation
- 1986-11-17 IE IE3028/86A patent/IE57866B1/en not_active IP Right Cessation
- 1986-11-17 NO NO864569A patent/NO175266C/no not_active IP Right Cessation
- 1986-11-17 IL IL80659A patent/IL80659A/xx not_active IP Right Cessation
- 1986-11-17 ZA ZA868689A patent/ZA868689B/xx unknown
- 1986-11-18 KR KR1019860009732A patent/KR920007666B1/ko not_active IP Right Cessation
1992
- 1992-10-22 GR GR920401405T patent/GR3006041T3/el unknown

Also Published As

Publication number	Publication date
NO175266C (no)	1994-09-28
KR870005094A (ko)	1987-06-04
EP0225286A2 (en)	1987-06-10
IL80659A (en)	1991-12-15
GR3006041T3 (fi)	1993-06-21
ES2043607T3 (es)	1994-01-01
KR920007666B1 (ko)	1992-09-14
ATE78514T1 (de)	1992-08-15
IE863028L (en)	1987-05-18
PT83752A (en)	1986-12-01
NO864569D0 (no)	1986-11-17
FI864654A0 (fi)	1986-11-14
PT83752B (pt)	1989-06-30
IE57866B1 (en)	1993-05-05
DE3686136D1 (de)	1992-08-27
EP0225286B1 (en)	1992-07-22
AU598490B2 (en)	1990-06-28
NO864569L (no)	1987-05-19
JPS62272976A (ja)	1987-11-27
DE3686136T2 (de)	1992-12-17
GB8528321D0 (en)	1985-12-24
EP0225286A3 (en)	1987-10-07
NZ218305A (en)	1990-01-29
AU6520086A (en)	1987-05-21
FI91160B (fi)	1994-02-15
ZA868689B (en)	1987-06-24
DK549186D0 (da)	1986-11-17
DK549186A (da)	1987-05-19
IL80659A0 (en)	1987-02-27
DK174977B1 (da)	2004-04-05
FI91160C (fi)	1994-05-25
NO175266B (no)	1994-06-13

Publication	Publication Date	Title
FI864654A (fi)	1987-05-19	Menetelmä modifioidun ihmisen t-PA-proteiinin valmistamiseksi
GR78180B (fi)	1984-09-26
DK166087D0 (da)	1987-04-01	Proteiner og fremgangsmaade til fremstilling deraf
DK275788A (da)	1989-02-03	Fibrinolytiske proteiner
FI864552A0 (fi)	1986-11-10	Human-lysozym.
DK203087D0 (da)	1987-04-21	Proteiner og fremgangsmaade til fremstilling deraf

Legal Events

Date	Code	Title	Description
1994-01-17	BB	Publication of examined application
1997-08-26	PC	Transfer of assignment of patent	Owner name: NOVARTIS AG
2004-08-31	FG	Patent granted	Owner name: NOVARTIS AG
2006-05-31	MA	Patent expired