FI79644B - SYNERGISTIC TILLVAEXTBEFODRANDE FODERTILLSATSAEMNE FOER BOSKAP. - Google Patents

Description

1 79644

Eynergic digestive feed additive composition for livestock

The invention relates to a synergistic growth-promoting feed additive composition for livestock, consisting essentially of an effective amount of 2-formylinyl-1,4-dioxide cyanoacetylhydrazone, commonly known as cyadox, together with certain biologically active additives. The invention also relates to the use of this feed additive composition and to feed mixtures for feeding livestock, such as pigs, calves and poultry, containing a corresponding additive. The composition of the invention can be used to improve weight gain and feed utilization.

It is known in the literature (CS 195 50Θ, DE 2 829 5Θ0, US 4 225 604) that cyadox effectively promotes the growth of livestock and inhibits both gram-positive and gram-negative microorganisms; it also apparently has significant coccidiostatic properties. In addition, the compound is almost non-toxic: in rats it has an LD50 of 15 g / kg p.o.

These beneficial growth and health properties of cyadox have recently been found to increase surprisingly when combined with antibiotics and / or suonphonamides, even those which have little or no growth-promoting effect per se. The observed increase in activity is substantially greater than would have been expected based on the known properties of the components. The desired effects produced by Cyadox alone are significantly increased in the above combination because the added components act synergistically to stimulate growth as well as inhibit pathogenic microorganisms. This synergy, as well as the unexpectedly low toxicity and the improved stability of cyadox, are among the specific properties of the substance.

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When test groups of early weaned piglets were treated in simultaneous experiments and otherwise identical conditions with cyadox and tylosin alone and a combination of both at the same dose level of 100 mg per kg of compound feed, weight gain as well as feed utilization in the combination group the sum of the effects obtained. Also, when pigs were fed cyadox, and / or a phthalazole-containing additive mixture at a total dose of 100 mg per kg in doses of 50 mg / kg, administered alone or in combination, at the initial stage of fattening, the corresponding effects of the combination were significantly greater than in the untreated control group. the sum of the effects of individual doses. This clearly shows synergies that could not have been expected on the basis of the available data.

The feed additive composition according to the invention contains the antibiotic and / or sulfonamide component in a suitable dosage form such as, for example, the corresponding acidic, basic or amphoteric compound, acid addition salt, base addition salt, complex salt or meta-11a. The antibiotic may also be present as a dried fermentation medium or as a previously antibiotic-containing culture containing the antibiotic. In this case, the term tylosin means a base or an equivalent amount of an acid addition salt such as hydrochloride, sulfate, phosphate and tartrate, or a crop containing dried tylosin.

Due to the very low toxicity of Cyadox and the antibiotics and su1 phonamides listed above, the administration of the feed additive composition does not involve the risk of dangerous effects even in the event of an overdose of the compound feed containing the additive.

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The eynergic growth-promoting feed additive composition according to the invention may contain 2-formylquinoxaline-1,4-dioxide-cyanoacetylhydrazone together with tylosin and / or phthalazole, the weight ratio of 2-formylquinoxaline-1,4-dioxide cyanoacetylhydrazone to tylosellol and / or phthalazine 0.1 and 10, as well as conventional feed carriers and / or excipients. However, to ensure balanced effects and effective synergy, a harmonic ratio of 1: 1 is preferred. The composition may contain both an antibiotic and a sulfonamide. When preparing compound feeds containing an additive, the composition is preferably added as a premix to nutritionally balanced compound feeds together with the desired amount of essential biologically active substances (biofactors) in an amount of substantially 20-500 g per ton of feed.

The essential features of the invention are set out in claim 1.

Suitable dosage forms may be solid or liquid such as granules, capsules, tablets, oral pastes, suspensions and the like. They may contain suitable solvents and diluents, organic or inorganic carriers such as limestone powder, kaolin, talc, starch, sugar, cellulose, crushed vegetable waste or similar substrates used in the preparation of pharmaceutical preparations, as well as conventional excipients (excipients) if desired, other biologically active components. Particular emphasis should be placed on the addition of suitable surfactants, such as cationic, anionic and nonionic surfactants and surfactant mixtures, which have good wetting and dispersing or emulsifying properties. These surfactants, preferably selected from a large number of commercial products, can also part of premixtures, feed additives and compound feed. The surfactants are preferably added in an amount of about 0.5 to 10.0 kg per ton of feed.

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Said forms of administration of the composition are prepared in the same way as the usual biofactor concentrates, premixes, feed additives or feed mixtures containing the additive by any known method, preferably by mixing or grinding the active ingredients with other components such as solvents, carriers and surfactants.

The feed additive composition according to the invention promotes the growth of livestock substantially more than its individual components and significantly improves the utilization of the feed. This feed additive composition also improves the general condition of the herd, reduces losses due to early death and thus increases the efficiency of livestock farming, especially in large-scale rearing of pigs, cattle and poultry.

The following examples illustrate the use and effect of the invention, but do not limit the invention in any way. The percentages used in the examples refer to percentages by weight.

Example 1

Early weaned piglets crossed between large white and landrasse breeds and born within 6 days were transferred from the distribution station to the experimental station on the fifth day after weaning. The animals were divided into groups of 8 and 12 piglets, and each group was placed in a separate pen; the pens had a slatted floor and were heated through wooden floorboards. After a 7-day adjustment period, the experiment was started. Throughout the 4-week tea period, all test groups were fed a commercial compound mixture v COS-2 designed to feed early weaned piglets. The following premix was added to the feed: 5 79644

Group I - (control group) mixture as such II - cyadox, 100 ppm <100 mg per kg of mixture) III - feed-grade tyloeine, 100 ppm pure base IV - cyadox and feed-grade tyloeine, 100 ppm each.

Animals were weighed individually at both the beginning and end of the experiment (day 28). The group's feed consumption was monitored daily and the feed utilization rate was calculated. The general health of the animals was also examined daily. The test results are summarized in the following Table I.

Table

Group I II III IV

Dose, Comparison Cyadox Tylosin Cyadox (100) ppm (0) (100) (100) + tylosin (100)

Number of animals 12 12 11 5x8

Initial weight 10.30 10.28 9.13 7.10 x, kg

Weight gain- 100.0 109.8 125.9 150.6% index

For feed - 2.0 1.99 1.90 1.61 use x (consumption per unit achieved)

For feed- 100.0 99.5 92.2 84.3 * use index (-0.5) (-7.8) (-15.7) β 79644

Similar results were obtained using tylosin in combination with cyadox as feed additives for pigs, calves and broilers.

Example 2

A diarrhea occurrence was observed in pigs' rearing station when 5-15% BU x PC + L piglets with an average live weight of 50 kg and placed in pens of 10-15 animals were reared. The disease was identified histologically and microbiologically; the pathogen was identified as Vibrio coli.

The experiments were divided into four groups and fed a commercial SOL compound mix with the following premix added:

Group I - (control group) mixture as such II - phthalazole, 100 ppm III - cyadox, 100 ppm IV - cyadox and phthalazole, 100 ppm each.

Said biologically active substances were administered on 14 days. Thereafter, there were no cases of diarrhea in groups III (cyadox) and IV (cyadox + phthalazole) (100% therapeutic efficacy), whereas in groups I (control) and II (phthalazole alone) the disease was still observed in 20–30% of the animals. At the end of the experiment, all 20 pigs from both groups (15 from Group I and 5 from Group II) had to be sent for emergency slaughter due to their poor condition. The test results are summarized in the following Table II.

Similar results were obtained when phthalazole was used in combination with cyadox as an additive in compound feed for pigs, calves and broilers.

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Claims (4)

1. Ti 11växtbefrämjande f Oderti11 sateämne för boskap, kännetecknat av attine detehehlener 2-formylkinoxa1 in-1,4-dioxidcyanoacetylhydrazone in combination with tylosin och / eller phthalazole, varvid viktförh & llandet mellan 2-formyl eller phthalazole ligger mellan 0,1 och 10, velvet sedvanliga fodrets bärarämne och / eller hjälpmedel. Growth-promoting livestock feed additive, characterized in that it contains 2-formylquinoxaline-1,4-dioxide cyanoacetylhydrazone together with tylosin and / or phthalazole, 2-formylquinoline-1,4-dioxide cyanoacetylhydrazone and a weight ratio of tylosin phthalazole ranging from 0.1 to 10, and conventional feed carriers and / or excipients. 2. The composition of the composition, in combination with the composition of the invention according to claim 1, comprises the composition of the active ingredient, in the form of 9 79644 and 20-500 g per tonne of a combination of the above-mentioned active ingredients. Growth-promoting compound feed to which the feed additive according to claim 1 has been added, characterized in that it contains said active components, each from 20 to 500 g per tonne, in addition to the customary feed additives and / or carriers. 3. Foderkompoeition enligt patentkravet 2, kännetecknad av att den deneh & ller phyeiologiskt ofarlig teneid 0.5-10 kg per ton. Compound feed according to Claim 2, characterized in that it comprises a physiologically harmless surfactant 0. 5.10 kg per tonne. Use of a feed additive according to claim 1 or a compound feed according to claim 2 or 3 for increasing the weight of animals and improving food utilization. 4. Användning av fodrets tillsateämne enligt patentkravet 1 eller foderkompoeition enligt patentkravet 2 eller 3 för utökning av djure vikt ocör förbättring av närings utnyttjande.