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ES8700868A1 - Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina. - Google Patents

Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina.

Info

Publication number
ES8700868A1
ES8700868A1 ES537146A ES537146A ES8700868A1 ES 8700868 A1 ES8700868 A1 ES 8700868A1 ES 537146 A ES537146 A ES 537146A ES 537146 A ES537146 A ES 537146A ES 8700868 A1 ES8700868 A1 ES 8700868A1
Authority
ES
Spain
Prior art keywords
factor viii
production
related products
obtaining
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES537146A
Other languages
English (en)
Other versions
ES537146A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genetics Institute LLC
Original Assignee
Genetics Institute LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=27068312&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=ES8700868(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from US06/546,650 external-priority patent/US4757006A/en
Application filed by Genetics Institute LLC filed Critical Genetics Institute LLC
Publication of ES8700868A1 publication Critical patent/ES8700868A1/es
Publication of ES537146A0 publication Critical patent/ES537146A0/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Toxicology (AREA)
  • Diabetes (AREA)
  • Public Health (AREA)
  • General Chemical & Material Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

METODO DE AISLAMIENTO D EUN FRAGMENTO DE GEN QUE CODIFICA POR LO MENOS UNA POSCION DE UNA PROTEINA. USANDO TECNICAS DE MANIPULACION DE GENES SE HA DESARROLLADO UN METODO QUE PERMITE LA PRODUCCION DE AHF MEDIANTE IDENTIFICACION Y CLONACION DEL GEN QUE COFDIFICA LA PROTEINA AHF HUMANA, CLONACION DE DICHO GEN, INCORPORACION DE ESTE EN UN VECTOR DE ADN RECOMBINANTE, TRANSFORMACION DE UN HUESPED ADECUADO CON EL VECTOR QUE INCLUYE EL CITADO GEN EXPRESION DEL GEN AHF HUMANO PRODUCIDO. ANALOGAMENTE SE PUEDE PRODUCIR AHF PORCINO. EL METODO PERMITE PRODUCIR <AHF> HUMANO MEDIANTE LAS TECNICAS DE ADN RECOMBINANTE QUE CODIFICA LA PRODUCCION CELULAR DE FACTOR VIII:C HUMANO DEFICITARIA O AUSENTE EN PACIENTES CON HEMOFILIA A.
ES537146A 1983-10-28 1984-10-26 Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina. Expired ES8700868A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/546,650 US4757006A (en) 1983-10-28 1983-10-28 Human factor VIII:C gene and recombinant methods for production
US64403684A 1984-08-24 1984-08-24

Publications (2)

Publication Number Publication Date
ES8700868A1 true ES8700868A1 (es) 1986-10-16
ES537146A0 ES537146A0 (es) 1986-10-16

Family

ID=27068312

Family Applications (4)

Application Number Title Priority Date Filing Date
ES537146A Expired ES8700868A1 (es) 1983-10-28 1984-10-26 Metodo de aislamiento de un fragmento de gen que codifica por lo menos una porcion de una proteina.
ES546655A Expired ES8704546A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de una proteina con actividad procoagu-lante del tipo de factor viii:c humano
ES546653A Expired ES8800987A1 (es) 1983-10-28 1985-09-02 Metodo de produccion del factor viii:c humano
ES546654A Expired ES8800359A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de factor viii:c humano

Family Applications After (3)

Application Number Title Priority Date Filing Date
ES546655A Expired ES8704546A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de una proteina con actividad procoagu-lante del tipo de factor viii:c humano
ES546653A Expired ES8800987A1 (es) 1983-10-28 1985-09-02 Metodo de produccion del factor viii:c humano
ES546654A Expired ES8800359A1 (es) 1983-10-28 1985-09-02 Metodo de produccion de factor viii:c humano

Country Status (9)

Country Link
EP (1) EP0162067B1 (es)
JP (4) JPH07106156B2 (es)
DK (1) DK174927B1 (es)
ES (4) ES8700868A1 (es)
FI (1) FI86436C (es)
IE (1) IE57884B1 (es)
IT (1) IT1177066B (es)
MX (1) MX9203437A (es)
WO (1) WO1985001961A1 (es)

Families Citing this family (40)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
US4703008A (en) * 1983-12-13 1987-10-27 Kiren-Amgen, Inc. DNA sequences encoding erythropoietin
KR850004274A (ko) * 1983-12-13 1985-07-11 원본미기재 에리트로포이에틴의 제조방법
US5004804A (en) * 1984-01-12 1991-04-02 Nordisk Gentofte Method and composition for preparation of factor VIIIC
US4716117A (en) * 1984-10-26 1987-12-29 Chiron Corporation Monoclonal antibodies to factor VIIIC
EP0466199B1 (en) * 1984-01-12 2002-09-18 Chiron Corporation Factor VIIIc encoding DNA sequences and related DNA constructs
US5045455A (en) * 1984-01-12 1991-09-03 Chiron Corporation Factor VIII:C cDNA cloning and expression
ZA852099B (en) * 1984-03-26 1985-12-24 Meloy Lab Recombinant factor viii-c.
IL74909A (en) * 1984-04-20 1992-01-15 Genentech Inc Preparation of functional human factor viii and dna sequences,expression vectors,transformed microorganisms and cell lines used therein
US4965199A (en) * 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
EP0182448A3 (en) * 1984-08-24 1987-10-28 Genetics Institute, Inc. Production of factor viii and related products
AU591671B2 (en) * 1985-04-11 1989-12-14 Children's Medical Center Corporation Von willebrand factor
US8597910B1 (en) 1985-04-11 2013-12-03 Children's Medical Center Corporation DNA encoding Von Willebrand Factor (VWF) and methods and cells for producing VFW, and VFW produced by the DNA, methods and cells
DE3531360A1 (de) * 1985-09-03 1987-05-07 Merck Patent Gmbh Dna-sequenzen, rekombinante dna-molekuele und verfahren zur herstellung des enzyms mutarotase aus acinetobacter calcoaceticus
JP2525022B2 (ja) * 1986-01-03 1996-08-14 ジェネティックス・インスチチュ−ト・インコ−ポレ−テッド ▲ＶＩＩＩ▼：ｃ因子型タンパク質の改良生産方法
US5198349A (en) * 1986-01-03 1993-03-30 Genetics Institute, Inc. Method for producing factor VIII:C and analogs
US5250421A (en) * 1986-01-03 1993-10-05 Genetics Institute, Inc. Method for producing factor VIII:C-type proteins
US5595886A (en) * 1986-01-27 1997-01-21 Chiron Corporation Protein complexes having Factor VIII:C activity and production thereof
FR2599754B1 (fr) * 1986-06-06 1989-12-29 Transgene Sa Procede de preparation de facteur viii a partir de cellules de mammiferes
DE3769872D1 (de) 1986-07-11 1991-06-13 Miles Inc Herstellung von rekombinantem protein.
WO1988005825A1 (en) * 1987-01-30 1988-08-11 Biogen N.V. A method for producing factor viii in high yield
CA1331157C (en) * 1987-04-06 1994-08-02 Randal J. Kaufman Method for producing factor viii:c-type proteins
US6060447A (en) * 1987-05-19 2000-05-09 Chiron Corporation Protein complexes having Factor VIII:C activity and production thereof
EP0294910B1 (en) 1987-06-12 1996-09-11 Immuno Ag Novel proteins with factor VIII activity, process for their preparation using genetically engineered cells and pharmaceutical compositions containing them
ES2112255T3 (es) * 1989-11-17 1998-04-01 Novo Nordisk As Complejos proteicos que tienen actividad del factor viii:c y su produccion.
US5155218A (en) 1990-05-08 1992-10-13 Neurogenetic Corporation Dna encoding human 5-ht1d receptors
DK53792D0 (da) * 1992-04-24 1992-04-24 Novo Nordisk As Fremgangsmaade til fremstilling af proteiner
US5563045A (en) * 1992-11-13 1996-10-08 Genetics Institute, Inc. Chimeric procoagulant proteins
US5910481A (en) * 1995-11-13 1999-06-08 Immuno Ag Hybrid proteins with modified activity
US6200560B1 (en) 1998-10-20 2001-03-13 Avigen, Inc. Adeno-associated virus vectors for expression of factor VIII by target cells
US6221349B1 (en) 1998-10-20 2001-04-24 Avigen, Inc. Adeno-associated vectors for expression of factor VIII by target cells
ES2648241T3 (es) 2003-09-30 2017-12-29 The Trustees Of The University Of Pennsylvania Clados de virus adenoasociados (AAV), secuencias, vectores que contienen el mismo, y usos de los mismos
WO2005123928A1 (en) * 2004-06-08 2005-12-29 Battelle Memorial Institute Production of human coagulation factor viii from plant cells and whole plants
ES2525143T3 (es) 2005-04-07 2014-12-18 The Trustees Of The University Of Pennsylvania Cápsides de AAVrh.64 modificado, composiciones que las contienen y usos de las mismas
US9315825B2 (en) 2010-03-29 2016-04-19 The Trustees Of The University Of Pennsylvania Pharmacologically induced transgene ablation system
CN102869779A (zh) 2010-03-29 2013-01-09 宾夕法尼亚大学托管会 药理学诱导的转基因消融系统
CN103502458B (zh) 2011-02-17 2016-11-16 宾夕法尼亚大学托管会 用于改变组织特异性和改善aav9-介导的基因转移的组合物和方法
US9719106B2 (en) 2013-04-29 2017-08-01 The Trustees Of The University Of Pennsylvania Tissue preferential codon modified expression cassettes, vectors containing same, and uses thereof
CN108093639B (zh) 2015-04-16 2022-07-19 埃默里大学 用于肝脏中蛋白质表达的重组启动子和载体及其用途
CN116209430A (zh) 2020-08-07 2023-06-02 阿米库斯治疗学公司 囊泡靶向蛋白及其用途

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US602312A (en) * 1898-04-12 eades
US4361509A (en) * 1981-12-14 1982-11-30 Scripps Clinic And Research Foundation Ultrapurification of factor VIII using monoclonal antibodies
US4663280A (en) * 1983-05-19 1987-05-05 Public Health Research Institute Of The City Of New York Expression and secretion vectors and method of constructing vectors
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
EP0466199B1 (en) * 1984-01-12 2002-09-18 Chiron Corporation Factor VIIIc encoding DNA sequences and related DNA constructs
ZA852099B (en) * 1984-03-26 1985-12-24 Meloy Lab Recombinant factor viii-c.
IL74909A (en) * 1984-04-20 1992-01-15 Genentech Inc Preparation of functional human factor viii and dna sequences,expression vectors,transformed microorganisms and cell lines used therein

Also Published As

Publication number Publication date
JPH02227078A (ja) 1990-09-10
IT1177066B (it) 1987-08-26
FI852547A0 (fi) 1985-06-27
FI86436B (fi) 1992-05-15
DK174927B1 (da) 2004-03-01
JPH0690763A (ja) 1994-04-05
FI852547L (fi) 1985-06-27
IT8423351A0 (it) 1984-10-26
JP2549049B2 (ja) 1996-10-30
JPH07106156B2 (ja) 1995-11-15
ES8800359A1 (es) 1987-10-16
IE57884B1 (en) 1993-05-05
JPS61500646A (ja) 1986-04-10
ES546653A0 (es) 1987-12-16
WO1985001961A1 (en) 1985-05-09
EP0162067B1 (en) 1992-07-15
EP0162067A1 (en) 1985-11-27
ES546655A0 (es) 1987-04-01
MX9203437A (es) 1992-07-01
DK292385A (da) 1985-06-27
ES537146A0 (es) 1986-10-16
JPH02182188A (ja) 1990-07-16
DK292385D0 (da) 1985-06-27
EP0162067A4 (en) 1988-03-22
ES8800987A1 (es) 1987-12-16
ES546654A0 (es) 1987-10-16
FI86436C (fi) 1992-08-25
ES8704546A1 (es) 1987-04-01

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