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ES8503132A1 - Method and device for determination of antithrombin-III. - Google Patents

Method and device for determination of antithrombin-III.

Info

Publication number
ES8503132A1
ES8503132A1 ES525637A ES525637A ES8503132A1 ES 8503132 A1 ES8503132 A1 ES 8503132A1 ES 525637 A ES525637 A ES 525637A ES 525637 A ES525637 A ES 525637A ES 8503132 A1 ES8503132 A1 ES 8503132A1
Authority
ES
Spain
Prior art keywords
iii
thrombin
change
strip
material containing
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES525637A
Other languages
Spanish (es)
Other versions
ES525637A0 (en
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of ES8503132A1 publication Critical patent/ES8503132A1/en
Publication of ES525637A0 publication Critical patent/ES525637A0/en
Granted legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03F—PHOTOMECHANICAL PRODUCTION OF TEXTURED OR PATTERNED SURFACES, e.g. FOR PRINTING, FOR PROCESSING OF SEMICONDUCTOR DEVICES; MATERIALS THEREFOR; ORIGINALS THEREFOR; APPARATUS SPECIALLY ADAPTED THEREFOR
    • G03F7/00—Photomechanical, e.g. photolithographic, production of textured or patterned surfaces, e.g. printing surfaces; Materials therefor, e.g. comprising photoresists; Apparatus specially adapted therefor
    • G03F7/70—Microphotolithographic exposure; Apparatus therefor
    • G03F7/70216—Mask projection systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/56—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving blood clotting factors, e.g. involving thrombin, thromboplastin, fibrinogen
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/52—Use of compounds or compositions for colorimetric, spectrophotometric or fluorometric investigation, e.g. use of reagent paper and including single- and multilayer analytical elements
    • G01N33/525—Multi-layer analytical elements
    • G01N33/526—Multi-layer analytical elements the element being adapted for a specific analyte
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/81—Protease inhibitors
    • G01N2333/8107—Endopeptidase (E.C. 3.4.21-99) inhibitors
    • G01N2333/811—Serine protease (E.C. 3.4.21) inhibitors
    • G01N2333/8121—Serpins
    • G01N2333/8128—Antithrombin III
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2400/00—Assays, e.g. immunoassays or enzyme assays, involving carbohydrates
    • G01N2400/10—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
    • G01N2400/38—Heteroglycans, i.e. polysaccharides having more than one sugar residue in the main chain in either alternating or less regular sequence, e.g. gluco- or galactomannans, Konjac gum, Locust bean gum or Guar gum
    • G01N2400/40—Glycosaminoglycans, i.e. GAG or mucopolysaccharides, e.g. chondroitin sulfate, dermatan sulfate, hyaluronic acid, heparin, heparan sulfate, and related sulfated polysaccharides
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/805—Test papers

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Neurosurgery (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

Disclosed is a method and device for the quantitative determination of AT-III in mammalian blood plasma. The method involves contacting a plasma sample containing excess heparin with a 3 layered reagent strip comprising: i. a first upper layer of a bibulous material containing thrombin and a buffer ii. a second lower layer of a bibulous material containing a fluorogenic or chromogenic substrate capable of interacting with thrombin in a time related chemical manner and iii. a bottom layer of water impermeable material. The chemical change in the strip is determined as a function of time and this change is compared with that obtained in samples containing known amounts of AT-III.
ES525637A 1982-09-15 1983-09-15 PROCEDURE FOR THE QUANTITATIVE DETERMINATION OF ANTITROMBINE-III IN BLOOD PLASMA OF MAMMALS Granted ES525637A0 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US06/418,285 US4473639A (en) 1982-09-15 1982-09-15 Reagent strip test for antithrombin-III

Publications (2)

Publication Number Publication Date
ES8503132A1 true ES8503132A1 (en) 1985-02-01
ES525637A0 ES525637A0 (en) 1985-02-01

Family

ID=23657466

Family Applications (1)

Application Number Title Priority Date Filing Date
ES525637A Granted ES525637A0 (en) 1982-09-15 1983-09-15 PROCEDURE FOR THE QUANTITATIVE DETERMINATION OF ANTITROMBINE-III IN BLOOD PLASMA OF MAMMALS

Country Status (8)

Country Link
US (1) US4473639A (en)
EP (1) EP0103247B1 (en)
JP (1) JPS59131166A (en)
AU (1) AU541754B2 (en)
CA (1) CA1187387A (en)
DE (1) DE3371364D1 (en)
ES (1) ES525637A0 (en)
IL (1) IL68674A (en)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4543335A (en) * 1982-12-20 1985-09-24 Miles Laboratories, Inc. Device and method for the quantitative determination of heparin in mammalian blood plasma
CA1254119A (en) * 1984-07-20 1989-05-16 Lloyd A. Schick Single step protease inhibitor assay
DE3516579A1 (en) * 1984-11-19 1986-05-22 Boehringer Mannheim Gmbh, 6800 Mannheim Coagulation test on test strips
JPS61181400A (en) * 1984-11-19 1986-08-14 ベ−リンガ−・マンハイム・ゲゼルシヤフト・ミツト・ベシユレンクテル・ハフツング Dry reagent for blood coagulation test
DE3616496A1 (en) * 1986-05-16 1987-11-19 Boehringer Mannheim Gmbh ANALYZING ELEMENT FOR DETERMINING COAGLING PARAMETERS
US5110727A (en) * 1987-04-03 1992-05-05 Cardiovascular Diagnostics, Inc. Method for performing coagulation assays accurately, rapidly and simply, using dry chemical reagents and paramagnetic particles
US5320945A (en) * 1989-07-14 1994-06-14 Boehringer Mannheim Gmbh Method and reagent for the determination of antithrombin III
DE3923340A1 (en) * 1989-07-14 1991-01-24 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR DETERMINING ANTITHROMBIN III
CA2070813C (en) * 1990-11-05 1996-10-29 Hendrik Coenraad Hemker A method to determine the concentration of anticoagulants
JPH078298A (en) * 1993-06-28 1995-01-13 Nippon Shoji Kk Method for measuring activity of antithrombin iii and reagent kit for the same measurement
JP3927425B2 (en) * 1996-11-21 2007-06-06 積水化学工業株式会社 Blood coagulation promoter and blood test container
JP3927403B2 (en) * 1996-11-21 2007-06-06 積水化学工業株式会社 Blood coagulation promoter and blood test container
JP3401162B2 (en) * 1996-11-21 2003-04-28 積水化学工業株式会社 Blood coagulation accelerator and blood test container
US5985582A (en) * 1997-12-09 1999-11-16 Sigma-Aldrich Co. Thrombin-based assay for antithrombin III
GB2426334A (en) 2005-05-20 2006-11-22 Orion Diagnostica Oy Application of a reagent to a matrix material
CN103604766A (en) * 2013-11-25 2014-02-26 牡丹江友搏药业股份有限公司 Biological quality control method of Shuxuetong injection

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4247454A (en) * 1975-07-11 1981-01-27 Ab Kabi Novel chromogenic thrombin substrates
US4061625A (en) * 1975-07-11 1977-12-06 Ab Kabi Novel chromogenic thrombin substrates
DE2812943C3 (en) * 1978-03-23 1981-05-14 Boehringer Mannheim Gmbh, 6800 Mannheim Method and reagent for determining the biological activity of heparin in plasma
US4302538A (en) * 1978-03-27 1981-11-24 Ortho Diagnostics Inc. Buffer system in an anti-thrombin III test
US4275153A (en) * 1978-08-03 1981-06-23 American Hospital Supply Corporation Analytical fluorogenic substrates for proteolytic enzymes
US4221706A (en) * 1979-06-14 1980-09-09 Abbott Laboratories Chromogenic substrates
US4324858A (en) * 1980-06-16 1982-04-13 Midwest Research Institute Stabilization of cholinesterase, detector kit using stabilized cholinesterase, and methods of making and using the same
US4390343A (en) * 1981-07-06 1983-06-28 Miles Laboratories, Inc. Multilayer analytical element having an impermeable radiation diffusing and blocking layer
US4363874A (en) * 1981-08-07 1982-12-14 Miles Laboratories, Inc. Multilayer analytical element having an impermeable radiation nondiffusing reflecting layer

Also Published As

Publication number Publication date
EP0103247A2 (en) 1984-03-21
IL68674A (en) 1986-02-28
JPH0322948B2 (en) 1991-03-27
AU1466383A (en) 1984-03-22
AU541754B2 (en) 1985-01-17
CA1187387A (en) 1985-05-21
US4473639A (en) 1984-09-25
JPS59131166A (en) 1984-07-27
DE3371364D1 (en) 1987-06-11
EP0103247B1 (en) 1987-05-06
EP0103247A3 (en) 1985-10-23
ES525637A0 (en) 1985-02-01

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19970303