ES398016A1 - Process for isolating a fibrin-stabilizing factor - Google Patents

Abstract

Procedure for the isolation of a fibrin stabilizing factor, characterized in that a) an extract released from solid impurities, prepared by means of NaCl solution from human placentas, is mixed at pH 5.0-7.5, preferably 6.0 with an approximately 3% solution of diaminoethoxyacridine lactate; b) decompose the separated precipitate in a neutral aqueous medium with 1.5-3%, preferably 2%, of alkali metal chloride, preferably NaCl, add 12-20% by volume of a lower alcohol, preferably ethanol, and separates the precipitate; c) the supernatant portion is clarified, by adding water the concentration of alkali metal chloride is decreased to (see formula) preferably approximately 1%, the solution is mixed at -5 to + 5ºC, preferably 0ºC, and pH 5.5-6.5, preferably 5.8, with 12-20% of a lower alcohol, preferably ethanol ; d) dissolves at about 0ºC; the residue in neutral buffer of ethylenediaminetetraacetic acid and alkali metal hydroxide, which may also contain small amounts of NaCl, NaN3 and glucose, an eventually remaining insoluble residue is removed and the solution is fractionated by gel filtration; e) the active fractions are chromatographed on cellulose, eluted with diluted neutral NaCl solution containing a small amount of epsilon-aminocaproic acid, and the eluate is dialyzed against neutral buffer, at 0.01-0.02%, preferably 0.015%, of sodium phosphate, containing a small amount of glucose; f) the dialyzed solution is optionally mixed with a stabilizer, filtered in sterile medium, normalized and dried by lyophilization. (Machine-translation by Google Translate, not legally binding)