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ES381368A1 - A method of cultivating a genero of heteroploid human epithelial hepatic cells. (Machine-translation by Google Translate, not legally binding) - Google Patents

A method of cultivating a genero of heteroploid human epithelial hepatic cells. (Machine-translation by Google Translate, not legally binding)

Info

Publication number
ES381368A1
ES381368A1 ES381368A ES381368A ES381368A1 ES 381368 A1 ES381368 A1 ES 381368A1 ES 381368 A ES381368 A ES 381368A ES 381368 A ES381368 A ES 381368A ES 381368 A1 ES381368 A1 ES 381368A1
Authority
ES
Spain
Prior art keywords
genus
heteroploid
translation
cells
machine
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES381368A
Other languages
Spanish (es)
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Wellcome Foundation Ltd
Original Assignee
Wellcome Foundation Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wellcome Foundation Ltd filed Critical Wellcome Foundation Ltd
Publication of ES381368A1 publication Critical patent/ES381368A1/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10361—Methods of inactivation or attenuation
    • C12N2710/10364—Methods of inactivation or attenuation by serial passage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Virology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

A method of culturing a genus of heteroploid human epithelial liver cells, such as the genus wrl 68 defined hereinbefore, which forms individually separate islands or individual groups when grown in a culture medium, has a morphology that substantially resembles that of hepatocytes of the human liver and a generation time of not more than 24 hours, exhibits an increased production of glycogen in the presence of 1% glucose in the medium, and is capable of supporting viruses, or as the genus consigned in the american type culture collection with the registration number cl48, or a biological equivalent thereof constituted by a genus of cells of the same essentially identical behavior, comprising keeping the cells in a nutrient culture medium. (Machine-translation by Google Translate, not legally binding)
ES381368A 1969-07-03 1970-07-02 A method of cultivating a genero of heteroploid human epithelial hepatic cells. (Machine-translation by Google Translate, not legally binding) Expired ES381368A1 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GB3360569 1969-07-03
GB3997369 1969-08-11

Publications (1)

Publication Number Publication Date
ES381368A1 true ES381368A1 (en) 1973-07-01

Family

ID=26261938

Family Applications (1)

Application Number Title Priority Date Filing Date
ES381368A Expired ES381368A1 (en) 1969-07-03 1970-07-02 A method of cultivating a genero of heteroploid human epithelial hepatic cells. (Machine-translation by Google Translate, not legally binding)

Country Status (11)

Country Link
JP (1) JPS5218251B1 (en)
BE (1) BE752908A (en)
CA (1) CA999546A (en)
CH (1) CH549642A (en)
DE (1) DE2033131A1 (en)
DK (1) DK128790B (en)
ES (1) ES381368A1 (en)
FI (1) FI49627C (en)
FR (1) FR2059471A1 (en)
NL (1) NL7009795A (en)
SE (1) SE416959B (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5691264U (en) * 1979-12-17 1981-07-21
FR2543158B1 (en) * 1983-03-24 1985-11-15 Inst Nat Sante Rech Med MEDIUM FOR CULTURING ANIMAL CELLS WITHOUT SERUM, WITHOUT HORMONES AND WITHOUT GROWTH FACTORS AND METHODS OF PRIMARY CULTURE AND OF OBTAINING CELL LINES USING THE SAME
FR2545100B1 (en) * 1983-04-29 1985-12-20 Inst Nat Sante Rech Med PROCESS FOR OBTAINING CULTURES OF HUMAN HEPATOCYTES, THE CULTURES OBTAINED AND THEIR BIOLOGICAL AND BIOCHEMICAL APPLICATIONS
US4581331A (en) * 1983-09-29 1986-04-08 E. I. Du Pont De Nemours And Company Method for the rapid detection of virus and viral antigens

Also Published As

Publication number Publication date
FR2059471A1 (en) 1971-06-04
FI49627C (en) 1975-08-11
JPS5218251B1 (en) 1977-05-20
CH549642A (en) 1974-05-31
DK128790B (en) 1974-07-01
FR2059471B1 (en) 1974-02-22
BE752908A (en) 1971-01-04
SE416959B (en) 1981-02-16
FI49627B (en) 1975-04-30
NL7009795A (en) 1971-01-05
DE2033131A1 (en) 1972-02-24
CA999546A (en) 1976-11-09

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