ES358216A1 - Composition for the Determination of Enzymes - Google Patents
Composition for the Determination of EnzymesInfo
- Publication number
- ES358216A1 ES358216A1 ES358216A ES358216A ES358216A1 ES 358216 A1 ES358216 A1 ES 358216A1 ES 358216 A ES358216 A ES 358216A ES 358216 A ES358216 A ES 358216A ES 358216 A1 ES358216 A1 ES 358216A1
- Authority
- ES
- Spain
- Prior art keywords
- alanine
- solution
- colour reagent
- naphthylamide
- determination
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
- 239000000203 mixture Substances 0.000 title abstract 2
- 102000004190 Enzymes Human genes 0.000 title 1
- 108090000790 Enzymes Proteins 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 abstract 3
- 239000000243 solution Substances 0.000 abstract 3
- VCYBSQKYFOVMPG-REOHCLBHSA-N (2s)-2-aminopropanehydrazide Chemical compound C[C@H](N)C(=O)NN VCYBSQKYFOVMPG-REOHCLBHSA-N 0.000 abstract 2
- OAKJQQAXSVQMHS-UHFFFAOYSA-N Hydrazine Chemical compound NN OAKJQQAXSVQMHS-UHFFFAOYSA-N 0.000 abstract 2
- 150000003934 aromatic aldehydes Chemical class 0.000 abstract 2
- 239000007853 buffer solution Substances 0.000 abstract 2
- BGNGWHSBYQYVRX-UHFFFAOYSA-N 4-(dimethylamino)benzaldehyde Chemical compound CN(C)C1=CC=C(C=O)C=C1 BGNGWHSBYQYVRX-UHFFFAOYSA-N 0.000 abstract 1
- TYMLOMAKGOJONV-UHFFFAOYSA-N 4-nitroaniline Chemical compound NC1=CC=C([N+]([O-])=O)C=C1 TYMLOMAKGOJONV-UHFFFAOYSA-N 0.000 abstract 1
- DEFJQIDDEAULHB-UHFFFAOYSA-N Alanyl-alanine Chemical compound CC(N)C(=O)NC(C)C(O)=O DEFJQIDDEAULHB-UHFFFAOYSA-N 0.000 abstract 1
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 abstract 1
- 235000004279 alanine Nutrition 0.000 abstract 1
- 150000001294 alanine derivatives Chemical class 0.000 abstract 1
- 235000001014 amino acid Nutrition 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 230000008033 biological extinction Effects 0.000 abstract 1
- 239000000872 buffer Substances 0.000 abstract 1
- PXFUDSMGEYRNNC-LURJTMIESA-N l-alanine-4-nitroanilide Chemical compound C[C@H](N)C(=O)NC1=CC=C([N+]([O-])=O)C=C1 PXFUDSMGEYRNNC-LURJTMIESA-N 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 210000002966 serum Anatomy 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
- C12Q1/37—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving peptidase or proteinase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
A composition for the determination of amino acid arylamidase (leucinaminopeptidase) comprises (a) alanine hydrazide, alanine pnitroanilide or alanine-#-naphthylamide, (b) a buffer solution with a pH of 6.5 to 7.5, with or without (c) an aromatic aldehyde colour reagent, for example p-dimethylamino-benzaldehyde. In use, the sample, for example blood serum, is incubated with the substrate and the buffer solution. When alanine p-nitroanilide is used, the p-nitro-aniline formed is determined colorimetrically, optionally in a kinetic test. When alanine hydrazide or alanine-#-naphthylamide is used, the hydrazine or #-naphthylamine formed react with the aromatic aldehyde colour reagent and the solution is acidified to give a coloured salt, the extinction of which is measured. A test pack contains solutions of the alanine derivative and of the buffer, optionally mixed together, with or without a solution of the colour reagent.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB4119767A GB1176968A (en) | 1967-09-08 | 1967-09-08 | Composition for the Determination of Enzymes |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES358216A1 true ES358216A1 (en) | 1971-02-16 |
Family
ID=10418565
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES358216A Expired ES358216A1 (en) | 1967-09-08 | 1968-09-06 | Composition for the Determination of Enzymes |
Country Status (2)
| Country | Link |
|---|---|
| ES (1) | ES358216A1 (en) |
| GB (1) | GB1176968A (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS60164499A (en) * | 1984-02-07 | 1985-08-27 | Kyowa Medetsukusu Kk | Method for measuring enzymic activity |
| CH683616A5 (en) * | 1984-06-18 | 1994-04-15 | Univ Michigan | Identification of plaque bacteria |
-
1967
- 1967-09-08 GB GB4119767A patent/GB1176968A/en not_active Expired
-
1968
- 1968-09-06 ES ES358216A patent/ES358216A1/en not_active Expired
Also Published As
| Publication number | Publication date |
|---|---|
| GB1176968A (en) | 1970-01-07 |
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