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ES2579954T3 - Ensayo para el descubrimiento de fármacos basado en células diferenciadas in vitro - Google Patents

Ensayo para el descubrimiento de fármacos basado en células diferenciadas in vitro Download PDF

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Publication number
ES2579954T3
ES2579954T3 ES10010425.6T ES10010425T ES2579954T3 ES 2579954 T3 ES2579954 T3 ES 2579954T3 ES 10010425 T ES10010425 T ES 10010425T ES 2579954 T3 ES2579954 T3 ES 2579954T3
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ES
Spain
Prior art keywords
vitro
test
drug discovery
differentiated cells
discovery based
Prior art date
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Expired - Lifetime
Application number
ES10010425.6T
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English (en)
Inventor
Heribert Bohlen
Kristina Dr. Tressat
Andreas Ehlich
Silke Schwengberg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Ncardia AG
Original Assignee
Axiogenesis AG
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Application filed by Axiogenesis AG filed Critical Axiogenesis AG
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Publication of ES2579954T3 publication Critical patent/ES2579954T3/es
Anticipated expiration legal-status Critical
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    • G—PHYSICS
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5014—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing toxicity
    • A—HUMAN NECESSITIES
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    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
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    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/04—Inotropic agents, i.e. stimulants of cardiac contraction; Drugs for heart failure
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P9/00—Drugs for disorders of the cardiovascular system
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    • A—HUMAN NECESSITIES
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    • A61P9/00—Drugs for disorders of the cardiovascular system
    • A61P9/10—Drugs for disorders of the cardiovascular system for treating ischaemic or atherosclerotic diseases, e.g. antianginal drugs, coronary vasodilators, drugs for myocardial infarction, retinopathy, cerebrovascula insufficiency, renal arteriosclerosis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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    • G01N33/5023—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on expression patterns
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    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
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    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G01N33/5026—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects on cell morphology
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    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
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Abstract

Procedimiento para identificar y/u obtener un fármaco para la mejora o tratamiento de una enfermedad o para determinar la toxicidad de un compuesto que comprende: (a) poner en contacto una muestra de ensayo que comprende una célula diferenciada in vitro con una sustancia de ensayo que se va a cribar, en el que en dicha célula se induce la expresión de un fenotipo de enfermedad predefinido que básicamente corresponde a un fenotipo de una célula de una célula, tejido u órgano enfermos; y (b) determinar un cambio sensible del fenotipo en dicha muestra de ensayo, en el que un cambio sensible (i) que evita o retrasa el inicio o la progresión del fenotipo de enfermedad es indicativo de un fármaco útil; y (ii) que potencia el inicio o progresión del fenotipo de enfermedad es indicativo de la toxicidad del compuesto; siempre que dicha célula no provenga de un embrión humano.

Description

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Claims (1)

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ES10010425.6T 2004-05-11 2005-05-11 Ensayo para el descubrimiento de fármacos basado en células diferenciadas in vitro Expired - Lifetime ES2579954T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP04011214 2004-05-11
EP04011214 2004-05-11

Publications (1)

Publication Number Publication Date
ES2579954T3 true ES2579954T3 (es) 2016-08-17

Family

ID=34924969

Family Applications (3)

Application Number Title Priority Date Filing Date
ES10010425.6T Expired - Lifetime ES2579954T3 (es) 2004-05-11 2005-05-11 Ensayo para el descubrimiento de fármacos basado en células diferenciadas in vitro
ES05740642T Expired - Lifetime ES2354997T3 (es) 2004-05-11 2005-05-11 Ensayo para el descubrimiento de fármacos basado en células diferenciadas in vitro.
ES16165848T Expired - Lifetime ES2770067T3 (es) 2004-05-11 2005-05-11 Descubrimiento de fármacos basado en células diferenciadas in vitro

Family Applications After (2)

Application Number Title Priority Date Filing Date
ES05740642T Expired - Lifetime ES2354997T3 (es) 2004-05-11 2005-05-11 Ensayo para el descubrimiento de fármacos basado en células diferenciadas in vitro.
ES16165848T Expired - Lifetime ES2770067T3 (es) 2004-05-11 2005-05-11 Descubrimiento de fármacos basado en células diferenciadas in vitro

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US (6) US8318488B1 (es)
EP (3) EP3070174B1 (es)
JP (2) JP4814875B2 (es)
AT (1) ATE490306T1 (es)
CA (1) CA2565858C (es)
DE (1) DE602005025106D1 (es)
DK (2) DK3070174T3 (es)
ES (3) ES2579954T3 (es)
WO (1) WO2005108598A1 (es)

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* Cited by examiner, † Cited by third party
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JP5074921B2 (ja) 2004-04-07 2012-11-14 アキシオジェネシス エージー 非侵襲性生体外機能組織検定システム
ES2579954T3 (es) * 2004-05-11 2016-08-17 Axiogenesis Ag Ensayo para el descubrimiento de fármacos basado en células diferenciadas in vitro
US8568761B2 (en) * 2005-07-15 2013-10-29 Cormatrix Cardiovascular, Inc. Compositions for regenerating defective or absent myocardium
WO2008048371A2 (en) 2006-04-04 2008-04-24 Singulex, Inc. Methods and compositions for highly sensitive analysis of markers and detection of molecules
US7838250B1 (en) 2006-04-04 2010-11-23 Singulex, Inc. Highly sensitive system and methods for analysis of troponin
WO2008129560A2 (en) * 2007-04-20 2008-10-30 Stempeutics Research Private Limited An in vitro human embryonic model and a method thereof
US20110250184A1 (en) * 2008-12-16 2011-10-13 Onconova Therapeutics ,Inc. Methods for determining efficacy of a therapeutic regimen against deleterious effects of cytotoxic agents in human
US10386360B2 (en) 2009-03-13 2019-08-20 University Of Central Florida Research Foundation, Inc. Bio-microelectromechanical system transducer and associated methods
US9163216B1 (en) 2009-04-23 2015-10-20 University Of Central Florida Research Foundation, Inc. Method for culturing skeletal muscle for tissue engineering
US8815584B1 (en) 2009-04-23 2014-08-26 University Of Central Florida Research Foundation, Inc. Method of co-culturing mammalian muscle cells and motoneurons
US8385624B2 (en) 2009-05-13 2013-02-26 David J. Charlot Automated transient image cytometry
US8828721B1 (en) 2009-05-28 2014-09-09 University Of Central Florida Research Foundation, Inc. Method of myelinating isolated motoneurons
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