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ES2554561A1 - New baculovirus-derived promoters with high activity in baculovirus-based expression systems - Google Patents

New baculovirus-derived promoters with high activity in baculovirus-based expression systems Download PDF

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Publication number
ES2554561A1
ES2554561A1 ES201430914A ES201430914A ES2554561A1 ES 2554561 A1 ES2554561 A1 ES 2554561A1 ES 201430914 A ES201430914 A ES 201430914A ES 201430914 A ES201430914 A ES 201430914A ES 2554561 A1 ES2554561 A1 ES 2554561A1
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ES
Spain
Prior art keywords
baculovirus
expression systems
high activity
based expression
new
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES201430914A
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Spanish (es)
Other versions
ES2554561B1 (en
Inventor
Salvador HERRERO SENDRA
Agata Karolina JAKUBOWSKA
María MARTÍNEZ SOLÍS
José Ángel Martínez Escribano
Silvia Gómez Sebastián
Javier López Vidal
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alternative Gene Expression SL ALGENEX
Universitat de Valencia
Original Assignee
Alternative Gene Expression SL ALGENEX
Universitat de Valencia
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Alternative Gene Expression SL ALGENEX, Universitat de Valencia filed Critical Alternative Gene Expression SL ALGENEX
Priority to ES201430914A priority Critical patent/ES2554561B1/en
Publication of ES2554561A1 publication Critical patent/ES2554561A1/en
Application granted granted Critical
Publication of ES2554561B1 publication Critical patent/ES2554561B1/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/866—Baculoviral vectors

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Virology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invención pertenece al campo de la biotecnología y comprende secuencias de ácidos nucleicos, incluyendo promotores capaces de incrementar la calidad y eficacia de la producción de proteínas recombinantes. Específicamente, la presente invención se refiere a una secuencia de ácido nucleico adecuada para funcionar como un promotor de baculovirus que comprende: a. la secuencia SEQ ID NO.: 1 ó su secuencia complementaria; o b. una variante de cualquiera de las secuencias del apartado a) que posee al menos un 70% de identidad con dicha secuencia.The present invention belongs to the field of biotechnology and comprises nucleic acid sequences, including promoters capable of increasing the quality and efficiency of recombinant protein production. Specifically, the present invention relates to a nucleic acid sequence suitable to function as a baculovirus promoter comprising: a. the sequence SEQ ID NO.: 1 or its complementary sequence; or b. a variant of any of the sequences in part a) that has at least 70% identity with said sequence.

ES201430914A 2014-06-16 2014-06-16 New baculovirus-derived promoters with high activity in baculovirus-based expression systems Expired - Fee Related ES2554561B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
ES201430914A ES2554561B1 (en) 2014-06-16 2014-06-16 New baculovirus-derived promoters with high activity in baculovirus-based expression systems

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES201430914A ES2554561B1 (en) 2014-06-16 2014-06-16 New baculovirus-derived promoters with high activity in baculovirus-based expression systems

Publications (2)

Publication Number Publication Date
ES2554561A1 true ES2554561A1 (en) 2015-12-21
ES2554561B1 ES2554561B1 (en) 2016-09-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
ES201430914A Expired - Fee Related ES2554561B1 (en) 2014-06-16 2014-06-16 New baculovirus-derived promoters with high activity in baculovirus-based expression systems

Country Status (1)

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ES (1) ES2554561B1 (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021236908A2 (en) 2020-05-20 2021-11-25 Biomarin Pharmaceutical Inc. Use of regulatory proteins for the production of adeno-associated virus

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012168492A2 (en) * 2011-06-10 2012-12-13 Alternative Gene Expression S.L. Recombinant dna elements for the expression of recombinant proteins in a host cell
WO2014086981A1 (en) * 2012-12-07 2014-06-12 Alternative Gene Expression S.L. Baculovirus system for expressing proteins forming virus-like particles

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012168492A2 (en) * 2011-06-10 2012-12-13 Alternative Gene Expression S.L. Recombinant dna elements for the expression of recombinant proteins in a host cell
WO2014086981A1 (en) * 2012-12-07 2014-06-12 Alternative Gene Expression S.L. Baculovirus system for expressing proteins forming virus-like particles

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
HELDENS, J.G. et al., `A highly conserved genomic region in baculoviruses: sequence analysis of an 11.3 kbp DNA fragment (46.5-55.1 m.u.) of the Spodoptera exigua multicapsid nucleopolyhedrovirus¿, VIRUS RESEARCH, 1998, Vol. 55, No. 2, Págs 187-198, ISSN: 0168-1702, todo el documento. *
MILLÁN-LEIVA, A. et al., `Genome sequence of SeIV-1, a novel virus from the Iflaviridae family infective to Spodoptera exigua¿, JOURNAL OF INVERTEBRATE PATHOLOGY, 2012, Vol. 109, No. 1, Págs 127-133, ISSN: 0022-2011, doi: 10.1016/j.jip.2011.10.009, todo el documento. *
PASCUAL, L. et al., `The transcriptome of Spodoptera exigua larvae exposed to different types of microbes.¿, Insect BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2012, Vol. 42, No. 8, Págs 557-570, ISSN: 0965-1748 (print), ISSN: 1879-0240 (electronic), doi: 10.1016/j.ibmb.2012.04.003, todo el documento. *

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2021236908A2 (en) 2020-05-20 2021-11-25 Biomarin Pharmaceutical Inc. Use of regulatory proteins for the production of adeno-associated virus

Also Published As

Publication number Publication date
ES2554561B1 (en) 2016-09-30

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