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ES2524723T3 - Fructosil peptidil oxidasa y un sensor para analizar una proteína glucosilada - Google Patents

Fructosil peptidil oxidasa y un sensor para analizar una proteína glucosilada Download PDF

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Publication number
ES2524723T3
ES2524723T3 ES10751788.0T ES10751788T ES2524723T3 ES 2524723 T3 ES2524723 T3 ES 2524723T3 ES 10751788 T ES10751788 T ES 10751788T ES 2524723 T3 ES2524723 T3 ES 2524723T3
Authority
ES
Spain
Prior art keywords
glycosylated protein
nitrosoaniline
analyze
sensor
fructosyl
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES10751788.0T
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English (en)
Inventor
Koji Sode
Kazunori Ikebukuro
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HOFFMANN LA ROCHE
F Hoffmann La Roche AG
Ultizyme International Ltd
Original Assignee
HOFFMANN LA ROCHE
F Hoffmann La Roche AG
Ultizyme International Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HOFFMANN LA ROCHE, F Hoffmann La Roche AG, Ultizyme International Ltd filed Critical HOFFMANN LA ROCHE
Application granted granted Critical
Publication of ES2524723T3 publication Critical patent/ES2524723T3/es
Active legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/001—Enzyme electrodes
    • C12Q1/004—Enzyme electrodes mediator-assisted
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/72—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood pigments, e.g. haemoglobin, bilirubin or other porphyrins; involving occult blood
    • G01N33/721—Haemoglobin
    • G01N33/723—Glycosylated haemoglobin

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La utilización de fructosil peptidil oxidasa que comprende una secuencia de aminoácidos con al menos un 90% de identidad con la secuencia de aminoácidos que se indica en el Id. de Sec. Nº:1 y que posee un valor de Km frente a la fructosil valil histidina de 1 mM o inferior para determinar una proteína glucosilada en una muestra en presencia de un mediador de electrones, y dicho mediador de electrones es mPMS/DCIP (metilsulfato de 1-metoxi-5- metilfenazinio/ 2,6-dicloroindofenol), cPES (trifluoroacetato-1-(3-carboxi-propoxi)-5-etil-fenanzinio, NA BM31 1144 (clorhidrato de N,N-bis-(hidroxietil)-3-metoxi-nitrosoanilina, NA BM31 1008 (N,N-bis-hidroxi-etil-4-nitrosoanilina) o NN- 4-dimetil-nitrosoanilina.

Description

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Claims (1)

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ES10751788.0T 2009-08-03 2010-08-02 Fructosil peptidil oxidasa y un sensor para analizar una proteína glucosilada Active ES2524723T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP09009968 2009-08-03
EP09009968A EP2281900A1 (en) 2009-08-03 2009-08-03 Fructosyl peptidyl oxidase and sensor for assaying a glycated protein
PCT/EP2010/004707 WO2011015325A1 (en) 2009-08-03 2010-08-02 Fructosyl peptidyl oxidase and sensor for assaying a glycated protein

Publications (1)

Publication Number Publication Date
ES2524723T3 true ES2524723T3 (es) 2014-12-11

Family

ID=41448516

Family Applications (1)

Application Number Title Priority Date Filing Date
ES10751788.0T Active ES2524723T3 (es) 2009-08-03 2010-08-02 Fructosil peptidil oxidasa y un sensor para analizar una proteína glucosilada

Country Status (7)

Country Link
US (2) US8721853B2 (es)
EP (2) EP2281900A1 (es)
JP (1) JP5870027B2 (es)
CN (1) CN102575278B (es)
ES (1) ES2524723T3 (es)
HK (1) HK1172930A1 (es)
WO (1) WO2011015325A1 (es)

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CN105473715B (zh) * 2013-08-09 2020-02-18 龟甲万株式会社 修饰型阿马多里酶及其制造法、以及阿马多里酶的耐表面活性剂性提高剂和使用它的HbA1c测定用组合物
EP3061829B1 (en) 2013-10-25 2021-09-22 Kikkoman Corporation METHOD FOR MEASUREMENT OF HbA1c USING AMADORIASE THAT REACTS WITH GLYCATED PEPTIDE
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JP7157063B2 (ja) 2017-08-31 2022-10-19 キッコーマン株式会社 糖化ヘモグロビンオキシダーゼ改変体及び測定方法
WO2020113129A1 (en) * 2018-11-29 2020-06-04 Polymer Technology Systems, Inc. Systems and methods for electrochemical point-of-care detection of hemoglobin
CN113075143B (zh) * 2021-03-31 2023-01-03 奥研生物科技(南京)有限公司 一种糖化血红蛋白检测试剂及其检测方法

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Also Published As

Publication number Publication date
US8721853B2 (en) 2014-05-13
US20120202235A1 (en) 2012-08-09
CN102575278B (zh) 2014-02-19
JP5870027B2 (ja) 2016-02-24
CN102575278A (zh) 2012-07-11
EP2462239A1 (en) 2012-06-13
WO2011015325A1 (en) 2011-02-10
EP2281900A1 (en) 2011-02-09
EP2462239B1 (en) 2014-09-17
US20140227728A1 (en) 2014-08-14
HK1172930A1 (en) 2013-05-03
US8962271B2 (en) 2015-02-24
JP2013500728A (ja) 2013-01-10

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