ES2329931T3 - COMPOSITION DETERGENT LIQUID CONTAINING AN ENZYME. - Google Patents

Abstract

Liquid detergent composition that can be used in the form of an aerosol comprising (a) from 1 to 30% by volume of an alkyl polyglucoside, (b) from 0.01 to 3% by volume of a pure essential oil from plants, (c) from 0.1 to 20% by volume of a glycol ether, (d) from 0.01 to 10% by volume of an enzyme, the remainder being made up to 100% by volume, mainly water.

Description

Liquid detergent composition containing a enzyme.

Object of the invention

The present invention aims at a liquid detergent composition obtained from raw materials renewable and comprising an enzyme. The detergent composition is refers to a use in domestic and industrial applications.

Prior art

Detergent and cleaning products containing enzymes are known and are used in domestic applications, mainly in bleach and dishwasher applications, and in industry, for example the food industries, for floors, walls and on-site cleaning. However, in products for domestic use, the use of enzymes is less widespread. Indeed, the addition of enzymes increases the cost of these products. It also requires some expertise, in particular to prevent any risk of allergy. It is known that the addition of enzymes to a liquid detergent composition allows to rapidly degrade stains of biological origin and thus increases the cleaning power. Each type of enzyme has a preferred action. Lipases act on grease stains, amylases degrade starch, proteases degrade protein stains (blood, eggs, ...) and cellulases plant fibers. However, enzymes are products of biological, animal or plant origin, and may be unstable under certain conditions. In particular, in a dilute aqueous medium, certain enzymes, in particular hydrolases, are unstable.

These known detergent products use traditionally as surfactants, products from of petrochemicals. As an example, alcohol sulfates Primary (PAS) and linear alkylbenzenesulfonates (LAS) are constituents of detergent compositions from the petrochemical, and are widely used in the industry. To this respect, they resort to non-renewable raw materials, and therefore not ecological and development concerns are raised sustainable. In addition, LAS have a negative influence on the enzyme stability Renewable raw materials they comprise products of animal origin, such as cow tallow, and products of plant origin, such as oils from the Agriculture.

State of the art

It is known from WO 01/32818 a liquid detergent composition containing a surfactant based on saccharides, for example an alkyl polyglucoside, and enzymes such as cellulase, amylase and protease. Stabilization of the enzyme is obtained by a system consisting of (a) a compound containing boron and / or sodium formate and (b) an alcohol polyhydroxylated. However, this stabilization system does not contributes nothing to the detergent or germicidal power of the composition. In addition, the compositions of this document are of high viscosity and contain all less than 40% water. To this in this respect, they would not be suitable for use in the form of aerosol. In addition, boron is an element that presents risks for Human health, particularly for fertility.

It is known from WO 01/96519 a liquid detergent composition containing (a) a detergent, (b) 0.001 to 10% dissolved or dispersed protease, and (c) a system of protease stabilization comprising from 2 to 40% of at minus a saccharide chosen from the group consisting of disaccharides, trisaccharides, their derivatives and mixtures. However, this stabilization system contributes nothing to power detergent or germicidal power of the composition. In the variants of this invention using a boron compound as an agent stabilizer (in particular examples 2, 3 and 4), is present The same risk to human health. This liquid composition Detergent is preferably in concentrated form. The compositions of this document are either liquids that can pour either gels that can be poured or gels that can't They can pour. In any case, they may not be suitable for A spray-shaped use.

US 6,136,778 proposes a composition aqueous detergent comprising an essential oil, an agent surfactant to solubilize the essential oil and an enzyme. Without However, no indication is provided in this document at as for the stability of the enzyme that is introduced into this composition.

Among the cleaning products available in the market, a first class of products are known that comprise constituents from petrochemicals and that do not include enzymes Products of this type are generally effective and economical In proceeding the main constituents of the products of this kind of petrochemicals, these products do not participate in the reduction of the use of fossil resources and, in this sense, do not respond to development concerns sustainable. The absence of enzymes makes these products less effective in the treatment of spots of biological origin.

Other kind of cleaning products available in the market it is constituted by formulations mainly to basis of renewable raw materials, but that also do not include enzymes This class includes the products "Mousse salle de bains "and" Soft "of the applicant. These products are formulated mainly from raw materials of plant origin or mineral, not from petrochemicals and presenting Generally a good biodegradability. These products are, the most of the time, strongly diluted. However, neither They comprise enzymes, which limits their cleaning performance. Is desirable the presence of enzymes to improve the cleaning effect of biological spots. As discussed in the patent application WO 01/85625 of the applicant, the presence of enzymes in the cleaning products also has a favorable effect on the environment by reducing the load imposed on the stations sewage treatment plants However, the application of enzymes in a product detergent requires some specific expertise, particularly for solve the problem of enzyme stability, in particular in a diluted product.

Objectives of the invention

The present invention is intended to provide a liquid detergent composition, comprising exclusively or mainly products from renewable raw materials, and none or few products from petrochemicals, which It comprises at least one enzyme, which has good yields of cleaning, in which the enzyme is stable, and does not present the disadvantages of the prior art. In particular, this invention aims to provide a composition in which the enzyme it is stable, even in a diluted solution, and thus allows a use in aerosol form.

Summary of the invention

The present invention relates to a liquid detergent composition comprising

(to)

of the 1 to 30% by volume of an alkyl polyglucoside,

(b)

of the 0.01 to 3% by volume of an essential oil,

(C)

of the 0.1 to 20% by volume of a glycol ether,

(d)

of the 0.01 to 10% by volume of an enzyme,

the rest being constituted until 100% by volume mainly by water. Therefore, the composition according to the invention allows to reduce the consumption of fossil materials, and thus conserve the resources of planet.

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The alkyl polyglycoside is preferably chosen from the group consisting of a C8-C16 glycoside, a C12-C16 glycoside and mixtures thereof.

The essential oil is preferably chosen from the group consisting of orange terpene, the essential oils obtained from Citrus limonum , Citrus aurantium L., Citrus aurantium L. ssp. bergamia, of Cymbopogon martinii , of Thymus vulgaris and their mixtures.

The glycol ether is chosen from the group that consists of dipropylene glycol monomethyl ether, monomethyl ether of propylene glycol and mixtures thereof.

The enzyme is preferably chosen from among lipases, proteases, cellulases, hemicellulases, Amylases, β-glucanases, xylanases, Arabanasas and their mixtures.

The alkyl polyglycoside content is preferably comprised between 10% and 20% by volume.

The essential oil content is comprised preferably between 1% and 2% by volume.

The glycol ether content is comprised preferably between 5% and 15% by volume.

The enzyme content is comprised preferably between 1% and 3% by volume.

The composition has, in a preferred variant of the invention, a water content that is comprised between the 85% and 97% by volume, and thus allow use in the form of aerosol.

Therefore, the present invention relates also to the use of a composition according to the invention.

This composition can be used with a antifungal target.

Brief description of the figures

Figure 1 is a graph that represents the Germicidal properties and detergent effect of four detergent compositions

Figures 2a, 2b, 2c and 2d are photographs of cultures of untreated molds, and treated with a product of cleaning for the bathroom according to the invention, after 24 hours and 48 hours incubation, respectively.

Figure 3 is a set of photographs of cultures of untreated molds, and treated with compositions A and B, at the initial time, after 24 hours, 48 hours and 72 hours of incubation, respectively.

Figure 4 is a set of photographs of mold cultures without any products, and with compositions C and D, at the initial moment, after 24 hours, 48 hours and 168 hours (it is say one week) of incubation, respectively.

Figure 5 is a set of photographs of glass cups coated with a colored butter and treated with a C8-C16 glycoside at various concentrations (1st line) and a commercial detergent, alcohol ethoxylated fatty C12-C13, 8OE (2nd line).

Figure 6 is a set of photographs of glass cups coated with a colored butter and treated with, respectively, (a) a formulation according to the invention, MPR5, and representative type formulations of classes of products described above: (b) first base class petrochemical and (c) second class based on raw materials renewable.

Figure 7 is a set of photographs of glass cups coated with a colored butter and treated with (a) a base degreasing spray formulation mainly petrochemical representative of what exists in the market and (b) an aerosol according to the invention, after 40 minutes of soaking.

Figures 8 and 9 are graphs that represent the evolution of the enzymatic activity of various compositions liquid detergents, as a function of time.

Detailed description of a preferred embodiment of the invention

The applicant has discovered that, so unexpectedly, the use of renewable raw materials (MPR) had a stabilizing effect on enzymes, and that the yields of Cleaning improved relative to a known formulation. Between the yields of the formulations, are the effect detergent, germicidal effect and antifungal activity.

Within the framework of the invention, the activities enzymatic, and its stability over time have measured as follows: a specific substrate is placed in presence of the enzyme. This substrate is hydrolyzed in a product which absorbs light at a wavelength of 405 nm. The absorbance of a sample of this substrate, by means of a Thermospectronic spectrophotometer. The measured absorbance allows deduct the concentration in substrate hydrolysis product (law of Beer-Lambert), and the absorbance variation ΔA / min is proportional to enzyme activity considered.

The alkyl polyglycosides (abbreviated: APG) are compounds of general formula:

R-O- (G) x

in which R is a chain of linear or branched alkyl, and G is a monosaccharide such as glucose. These compounds are obtained from a fatty alcohol from a vegetable oil and the monosaccharide. Among the APGs commonly used and commercially available listed C8 to C16 fatty alcohols glycoside (referred to below C8-C18 glycoside) such as coconut glycoside and C12 to C16 glycoside fatty alcohols (referred to as then C12-C18 glycoside) such as the laurylglucoside

In the following table, formulation A is a classic formulation consisting of ethoxylated fatty alcohol, acid boric, glycol ether (dipropylene glycol monomethyl ether), enzymes (lipase) and preservatives. Formulation B is a formulation according to the invention, wherein the alkyl polyglycoside is a glycoside C8-C16, the glycol ether is monomethyl ether of dipropylene glycol, the essential oil is orange terpene and the Enzyme is a lipase. Lipase activity was measured after a Waiting period of 1 to 90 days. The results of these measurements show that enzymatic activity decreases significantly less fast in formulation B according to the invention than in classical formulation A, the activity being, on average, a 35% higher in formulation B.

one

Tests were conducted with formulations A and B above, in diluted form. The form diluted in water is interesting since it allows a use in the form of aerosol.

2

These results show that the formulations Classic enzymes cannot be supplied in diluted form, because they lose practically all their enzymatic activity, and by both must be diluted at the time of use.

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Germicidal and detergent effect

Germicidal and detergent effects were compared of four formulations:

(one)

White: urban water supply

(2)

Classic product: ethoxylated fatty alcohol + potash Caustic + phosphate + preservatives

(3)

Classic enzyme product: ethoxylated fatty alcohol + boric acid + glycol ether (monomethyl ether of dipropylene glycol) + enzymes (lipase) + preservatives

(4)

Enzymatic product according to the invention, stabilized by MPR: C8-C16 glycoside + glycol ether (dipropylene glycol monomethyl ether) + orange terpene + enzymes (lipase).

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Whose compositions are provided in the table next, in which the concentrations are expressed as a percentage in volume:

3

Cleaning performance can be assessed with Regarding two factors:

-

FROM (detergent effect): represents the degree of stain removal of organic origin of the surface. Its scale varies from 0 to 4. The higher its value, the more important is the level of surface cleaning.

-

GE (germicidal effect): represents the degree of elimination of microorganisms that adhere to a surface. Its scale is ranges from 0 to 8. The higher the value of GE, the more important is the number of microorganisms removed from the surface.

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The following table and figure 1 include the DE and GE values after surface cleaning with the help of The four formulations described:

5

In figure 1, a point represents the two characteristics, germicidal effect (GE) and detergent effect (DE), of each of the four formulations studied. The classic product and the classic enzyme product are in the area of cleaning (cleaning effect greater than 1.5). Enzymatic product according to the invention it is at the same time in the cleaning zone and in the disinfection zone (germicidal effect greater than 3). This figure represents by an arrow the improvement of these two effects in the formulations according to the invention. Therefore, the improvement of the yields of the enzyme product according to the invention.

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Antifungal activity

Activity tests have been carried out antifungal following the following operating mode:

A composition according to the invention contains the following components:

-

surfactants from renewable raw materials,

-

pure essential oils from plants (indicated in the following table by a.e. from...)

-

a glycol ether, the dipropylene glycol monomethyl ether,

-

enzymes

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The detailed composition in the table is provided next:

6

This composition is particularly adapted for the elimination and prevention of the appearance of molds, by example in the bathrooms.

Operating Mode :

The antifungal activity test is performed based on the antibiogram technique: a means of inoculating rich culture with the help of microorganisms on which it is desired What the product works:

-

inoculum: fungi collected in one place damp

-

be they pour 200 µl of product over the inoculated place

-

incubation for 48 hours at 35ºC

Results :

Figure 2a shows the development of the crop of molds after 24 hours, on an untreated support. Figure 2b It shows the same result, with the previous composition. The figures 2c and 2d show this development after 48 hours. After 24 hours of incubation, molds begin to develop on the plate of Petri in which no product was added. Instead, there is no no mold development on the region in which they were added 200 µl of the composition according to the invention. After 48 hours of incubation, molds continue to develop strongly on the inoculated medium in which no product was deposited. On the contrary, there is still no trace of mold on the plate in which the composition according to the invention was added. Do not However, some microorganisms develop around the halo of inhibition formed by the addition of the product. Therefore no There is no doubt that the composition of the invention has properties antifungal because it prevents fungi from developing during a determined period. This product should be used regularly if it is desired to use it as a preventive treatment. A study complementary demonstrated the action of both enzymes and Essential oils on the limitation of fungal growth. Therefore, each active substance is of interest.

The applicant has discovered that compositions according to the invention, comprising an APG, an oil essential, a glycol ether and an enzyme, have characteristics Unexpected interesting. To highlight this effect synergistically, the development of molds in Petri dishes was compared untreated (referred to below as "white") and in test compositions The following tests were performed with the following compositions:

(TO)

Product A contains an essential oil and an APG (a C8-C16 glycoside), but not an enzyme. The essential oils are the essential oils of Thymus vulgaris , Cymbopogon martinii , Citrus limonum (0.1%).

(B)

Product B, conversely, contains an enzyme, but not essential oil, and the same APG (a glycoside C8-C16). Enzymes are the alpha-amylase, an enzyme complex of β-glucanase, cellulase, hemicellulase, arabanase and xylanase, carbohydrase, lysophospholipase and phospholipase (0.2%)

(C)

A composition according to the invention, which contains an APG, an essential oil and an enzyme. APG is a C8-C16 glycoside, and the essential oil is a mixture of essential oils of Thymus vulgaris , Cymbopogon martinii and Citrus limonum (0.1%),

(D)

Another composition according to the invention, which contains an APG, an essential oil and an enzyme. APG is a C8-C16 glycoside, and the essential oil is a mixture of essential oils of Citrus limonum and Citrus aurantium L. ssp. Bergamia (0.1%) (essential oils obtained from citrus fruits).

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A volume of 200 µl of each is poured product on molds transferred to Petri dishes in rich medium. The inoculum is prepared on Petri dishes from a piece of agar already contaminated by molds. The plates are incubated Petri at 35 ° C for 72 hours and compared with a blank. Be observe the following results:

A first series of trials aims to evaluate the antifungal effect of each of the components of the Compositions according to the invention. Products A, B, C are compared and D, described above. Figure 3a shows the status of mold development at the initial moment. Figure 3b shows the state of mold development after 24 hours. It is observed that the mycelium of molds begins to spread circularly to from the place where the contaminant has been deposited. In the plate on which compositions A and B have been applied, it is not See no mold development. Figure 3c shows the status of mold development after 48 hours. After 48 hours of incubation, white molds have tripled its surface of coverage from the place of inoculation of departure. They have to observe some mold development points for the product A, which contains an essential oil and product B, which contains a enzyme, but this development is limited. Figure 3c shows the state of mold development after 72 hours. After 72 hours of incubation, the mycelium that constitutes the white molds has extended more. The mold points for product A and B have been developed but the amount of mycelium with respect to the target is restricted. In addition, molds do not develop from the place of inoculation (in the center) but rather from the periphery, indicating the presence of a zone of inhibition caused by the antifungal activity of the two products A and B.

A second series of trials aims to evaluate the antifungal effect of each of the two types essential oils used in the compositions according to the invention and compares the compositions C and D and the "white". Figure 4a shows the state of mold development at the initial moment. The figure 4b shows the state of mold development after 24 hours. After 24 hours incubation, molds begin to develop for White. There is no development for Petri dishes that contain compositions C and D. Figure 4c shows the status of mold development after 48 hours of incubation. Mycelium Blank content has acquired considerable breadth. Be begins to perceive a slight development of molds for compositions C and D but this development is much less pronounced than for the target This less important development manifests the antifungal activity of compositions C and D. Figure 4d shows the state of mold development after a week of incubation. After a week, the mycelium of molds has filled by Complete the entire control surface. The surface of the means in which compositions C and D have been deposited follow without being fully covered. In addition, mold development it is less pronounced on the side of composition C than on that of the composition D. This is completely normal since the oils Essentials based on aromatic plants (clove, palmarosa, etc ...) They are recognized for their bactericidal and antifungal power. The Essential oils based on citrus fruits (lemon, bergamot, etc ...) they have a bactericidal and antifungal power less pronounced.

It is concluded from these trials, by a part, that enzymes and essential oils (from aromatic plants) each have antifungal activity. By both these two types of renewable raw materials can function synergistically since they have modes of action Different about microorganisms. These results put of manifest an antifungal effect of the enzymes used in the Formulations of the invention. Then, the choice of the mixture of essential oils determines the effectiveness in a more or less term throughout antifungal activity.

Detergent effect

We sought to evaluate the effectiveness of constituents that are introduced into the compositions according to the invention, taken in isolation, comparing them with a product habitual. The experiment involves coating a glass cup with a fixed amount of red butter. It gets in contact the product to be tested with the cup, without agitation, in order to verify the degreasing effectiveness of the formula. In a first series of trials, whose results are represented in figure 5, the degreasing effect of a C8-C16 glycoside, which is a surfactant used in the compositions of the invention, with that of a ethoxylated fatty alcohol C12-C13, 8OE, product from petrochemicals, which is a surfactant commonly used in detergent compositions, at different concentrations. These two test surfactants are selected as they were effective surfactants, coming from one part of a plant origin and another part of Petrochemicals The results of the experiment, represented in Figure 5, show that these two surfactants are effective, with a significantly better effect for the surfactant of origin vegetable, particularly at the highest concentrations, 0.6 at 1%. In a second series of trials, not represented, He compared the efficacy of several solvents. The results of this Second series of trials are as follows:

-

the solvents that have the most important degreasing power, Among the range of solvents tested are oils essential orange and lemon terpenes, ethers of propylene glycol (propylene glycol monomethyl ether and monomethyl ether of dipropylene glycol).

These two series of trials also lead to The following conclusions:

-

the Solvents (essential oils and glycol ethers) act quickly on fat and then do not act anymore;

-

the surfactants (a C8-C16 glycoside) act more slowly but for a long time, until elimination of fat deposits.

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Therefore, the compositions according to the invention join the short-term degreasing effect of solvents with the longer term degreasing effect of glycosides C8-C16.

It also sought to highlight the effectiveness of the compositions according to the invention, comprising a alkyl polyglycoside, an essential oil, a glycol ether and a enzyme with respect to the two classes of known products of the prior art The experiment consists of covering a cup of glass with a fixed amount and red butter. Be puts the product to be tested in contact with the Cup, without agitation, in order to verify the effectiveness Degreaser of the formula. 50 ml of a solution is poured into 10% of the composition to be tested in the cup, is leave on for 40 minutes at room temperature, then Dry in an oven at 105ºC for 20 minutes to remove any trace of water, and weighed to determine the amount of butter Colored removed. The compositions according to the invention, MPRF1 a MPRF6, are compared with first class products, the product "Reatout" of the applicant, and a representative formulation of this class of products, and with products of the second class, the "Soft" product of the applicant, and a formulation representative of this other class of products. The compositions of Reatout and Soft products are the following:

40

The representative product compositions of the first and second classes are the following:

60

The effectiveness of each of the formulations, determined as the percentage by weight of colored butter removed, in the following table:

8

Figure 6 represents the evolution after 1 h of soaking. It is noted that the composition according to the invention MPR5 (Figure 6 a) has a more effective degreasing power than Class 1 market products (figure 6b) and 2 (figure 6c). This improvement of the degreasing power of a composition according to the invention can be explained by the synergy of the action of enzymes (lipase) and the other components of the product (glycoside C8-C16, glycol ether, essential oils).

Use in aerosol form

It has also sought to highlight the efficacy of a composition according to the invention that can be used in the form of an aerosol, that is, a strongly diluted composition with water, which comprises an alkyl polyglucoside, an essential oil, a glycol ether and an enzyme. The effectiveness of this composition is compare with that of a multi-purpose spray cleaning formulation representative of what exists in the market. The compositions of these two formulations and their effectiveness, determined as what is the percentage by weight of colored butter removed, detailed in the following table:

10

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The test is also performed in cups of glass coated with a thin layer of colored butter with Help of a red dye. A quantity is spread evenly 0.5 g of butter per cup over the entire surface. The compositions to be tested are contacted with the cups, without agitation, in order to verify the effectiveness of the formula. 50 ml of a pure solution of each is poured composition to be tested in the cup. This test of Efficiency is performed without mechanical action and at room temperature. The results are observed after 40 minutes (see Figure 7). From the first minutes, the aerosol according to the invention (figure 7b) attacks the fat while the product of the market (figure 7a) almost does not act. After 40 minutes, one has taken off larger amount of fat per spray according to the invention while a significant amount of fat material on the treated cup by means of the product of market. In addition, the aerosol of the invention emulsifies much better. the fat that the product of the market. PH variation, before and after the action of the product, for the composition according to the invention, it is 0.27, while it is null for the market product. Therefore, the emulsion obtained is acidified with the aerosol according to the invention. This indicates that lipases contained in the formulation itself that act on triglycerides transforming a part of the fat into fatty acids, leading to acidification of the mixture. In short, the spray novel according to the invention, based on MPR, has properties improved in relation to market products. Thank you to your powerful natural elements that work synergistically (C8-C16 glycoside, orange terpene, lipase), It manages to attack fatty substances effectively and quickly.

Stability of the enzymes

It has finally sought to highlight the stability characteristics of enzymes in various formulations Figure 8 represents the evolution of the Enzymatic activities of the compositions according to the invention MPRF1 to MPRF6, and of a classical enzymatic composition, without MPR, the product "Degres L" of the applicant. It is noted that in all the cases, the enzymatic activity of the compositions according to the invention is superior to that of the composition "Degres L". The stability of the enzyme has also been tested in various formulations listed in the following table. MPR1 no It contains neither essential oil, nor glycol ether. In MPR2, it has Glycol ether added: dipropylene glycol monomethyl ether. In MPR3, a terpene is present, but not glycol ether. In MPR4, no There is MPR. MPR5 is a composition according to the invention. MPR6 is comparable to MPR1, but the surfactant is a product commercial from the petrochemical industry. MPR7 contains a glycol ether and an enzyme.

12

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Figure 9 represents the evolution of the Enzymatic activities of these compositions. It is noted that Orange terpene (MPR4 composition) and a glycoside C8-C16 (MPR1 composition) are two MPRs that they stabilize the enzyme very strongly. It is also found that the dipropylene glycol monomethyl ether (MPR7 composition) provides better stability than ethoxylated fatty alcohol C12-C13, 8OE (MPR6 composition). Glycol ether It is present in the formulation since its degreasing role is indispensable. Finally, it is important to note that the activity enzymatic obtained with a composition containing a surfactant  100% vegetable (C8-C16 glycoside: MPR1) is better than that obtained with a 100% petrochemical surfactant (fatty alcohol ethoxylated C12-C13, 8OE: MPR7).

Claims (12)

1. Liquid detergent composition that can be used in aerosol form comprising

(to)

of the 1 to 30% by volume of an alkyl polyglucoside,

(b)

of the 0.01 to 3% by volume of a pure essential oil from plants,

(C)

of the 0.1 to 20% by volume of a glycol ether,

(d)

of the 0.01 to 10% by volume of an enzyme,

the rest being constituted until 100% in volume mainly by Water.

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2. Composition according to claim 1, characterized in that the alkyl polyglycoside is selected from the group consisting of a C8-C16 glycoside, a C12-C16 glycoside and mixtures thereof. 3. Composition according to any one of the preceding claims, characterized in that the essential oil is chosen from the group consisting of orange terpene, the essential oils obtained from Citrus limonum , Citrus aurantium L., Citrus aurantium L. ssp. bergamia, of Cymbopogon martinii , of Thymus vulgaris and their mixtures. 4. Composition according to any one of the preceding claims, characterized in that the glycol ether is selected from the group consisting of dipropylene glycol monomethyl ether, propylene glycol monomethyl ether and mixtures thereof. 5. Composition according to any one of the preceding claims, characterized in that the enzyme is chosen from

-

the lipases,

-

the proteases,

-

the cellulases,

-

the hemicellulases,

-

the Amylases,

-

the β-glucanases,

-

the xylanases,

-

the arabanasas

and their mixtures

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6. Composition according to any one of the preceding claims, characterized in that the alkyl polyglycoside content is comprised between 10% and 20% by volume. 7. Composition according to any one of the preceding claims, characterized in that the essential oil content is between 1% and 2% by volume. Composition according to any one of the preceding claims, characterized in that the glycol ether content is between 5% and 15% by volume. 9. Composition according to any one of the preceding claims, characterized in that the enzyme content is comprised between 1% and 3% by volume. 10. Composition according to any one of the preceding claims, for use in aerosol form, characterized in that the water content is comprised between 85% and 97% by volume. 11. Use of a composition according to any one of the preceding claims, in aerosol form. 12. Use according to claim 11, with a antifungal target.