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ES2168510T3 - Transformacion de plantas de algodon. - Google Patents

Transformacion de plantas de algodon.

Info

Publication number
ES2168510T3
ES2168510T3 ES96934071T ES96934071T ES2168510T3 ES 2168510 T3 ES2168510 T3 ES 2168510T3 ES 96934071 T ES96934071 T ES 96934071T ES 96934071 T ES96934071 T ES 96934071T ES 2168510 T3 ES2168510 T3 ES 2168510T3
Authority
ES
Spain
Prior art keywords
cotton
explant
tissue
transformation
cotton plants
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES96934071T
Other languages
English (en)
Inventor
Steven G Strickland
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Monsanto Co
Original Assignee
Calgene LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Calgene LLC filed Critical Calgene LLC
Application granted granted Critical
Publication of ES2168510T3 publication Critical patent/ES2168510T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/008—Methods for regeneration to complete plants
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Microbiology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Cosmetics (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Hydroponics (AREA)
  • Fertilizers (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

SE SUMINISTRA UN PROCEDIMIENTO PARA REGENERAR PLANTAS DE ALGODON A PARTIR DE TEJIDO EXTIRPADO. EL PROCEDIMIENTO MEJORADO PERMITE LA GENERACION DE UN CALLO EMBRIOGENICO A PARTIR DE UN TEJIDO DE ALGODON EXTIRPADO QUE NO SE CULTIVA EN UN MEDIO DE INICIACION DEL ALGODON QUE TIENE HORMONAS VEGETALES HEXOGENAS. EL PROCEDIMIENTO PUEDE UTILIZARSE EN LA TRANSFORMACION DE PLANTAS DE ALGODON, CORTANDO TEJIDO DE ALGODON PARA FORMAR UN TEJIDO EXTIRPADO Y MANTENIDO EN UN MEDIO DE CULTIVO, O CULTIVANDO EL TEJIDO EXTIRPADO CON AGROBACTERIUM QUE COMPRENDA UNA SECUENCIA DE ADN DE INTERES, Y CULTIVANDO EL TEJIDO EXTIRPADO CO-CULTIVADO SOBRE UN MEDIO DE INICIACION DEL ALGODON QUE COMPRENDA UN AGENTE SELECTIVO PERO QUE NO TENGA HORMONAS VEGETALES HEXOGENAS. DE ESTA FORMA LAS CELULAS TRANSFORMADAS SON INDUCIDAS A PRODUCIR CALLOS EMBRIOGENICOS SOBRE UN MEDIO SELECTIVO LIBRE DE HORMONAS.
ES96934071T 1995-10-04 1996-10-04 Transformacion de plantas de algodon. Expired - Lifetime ES2168510T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US08/539,176 US5846797A (en) 1995-10-04 1995-10-04 Cotton transformation

Publications (1)

Publication Number Publication Date
ES2168510T3 true ES2168510T3 (es) 2002-06-16

Family

ID=24150117

Family Applications (1)

Application Number Title Priority Date Filing Date
ES96934071T Expired - Lifetime ES2168510T3 (es) 1995-10-04 1996-10-04 Transformacion de plantas de algodon.

Country Status (11)

Country Link
US (1) US5846797A (es)
EP (1) EP0910239B1 (es)
CN (1) CN1198655A (es)
AT (1) ATE209853T1 (es)
AU (1) AU727910B2 (es)
DE (1) DE69617749T2 (es)
DK (1) DK0910239T3 (es)
ES (1) ES2168510T3 (es)
PT (1) PT910239E (es)
TR (1) TR199800654T1 (es)
WO (1) WO1997012512A2 (es)

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DE69617749D1 (de) 2002-01-17
AU727910B2 (en) 2001-01-04
DK0910239T3 (da) 2002-03-04
PT910239E (pt) 2002-04-29
WO1997012512A2 (en) 1997-04-10
EP0910239A2 (en) 1999-04-28
DE69617749T2 (de) 2002-08-08
US5846797A (en) 1998-12-08
AU7257996A (en) 1997-04-28
EP0910239B1 (en) 2001-12-05
WO1997012512A3 (en) 1997-05-22
CN1198655A (zh) 1998-11-11
ATE209853T1 (de) 2001-12-15

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