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ES2151901T3 - Metodo y aparato para propagar particulas de biomasa. - Google Patents

Metodo y aparato para propagar particulas de biomasa.

Info

Publication number
ES2151901T3
ES2151901T3 ES93903440T ES93903440T ES2151901T3 ES 2151901 T3 ES2151901 T3 ES 2151901T3 ES 93903440 T ES93903440 T ES 93903440T ES 93903440 T ES93903440 T ES 93903440T ES 2151901 T3 ES2151901 T3 ES 2151901T3
Authority
ES
Spain
Prior art keywords
culture medium
settling chamber
vessel
particle
agitated
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93903440T
Other languages
English (en)
Inventor
Kwan-Min Jem
Pierre-Francois Cevey
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Research Systems ARS Holding NV
Original Assignee
Applied Research Systems ARS Holding NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applied Research Systems ARS Holding NV filed Critical Applied Research Systems ARS Holding NV
Application granted granted Critical
Publication of ES2151901T3 publication Critical patent/ES2151901T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/02—Stirrer or mobile mixing elements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22—Settling tanks; Sedimentation by gravity

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Abstract

LA PRESENTE INVENCION SE REFIERE A UN METODO Y APARATO MEJORADOS DE CRECIMIENTO DE PARTICULAS DE BIOMASA (15), PARTICULARMENTE CELULAS ENLAZADAS DE MICROPORTADORAS, EN UN RECIPIENTE DE CULTIVO EN SUSPENSION (10) EN EL CUAL SE AÑADE UN MEDIO DE CULTIVO FRESCO Y EL MEDIO DE CULTIVO GASTADO SE RETIRA CONTINUA O SEMICONTINUAMENTE. LA MEJORA CONSISTE EN RETIRAR EL MEDIO DE CULTIVO GASTADO A TRAVES DE UNA CAMARA DE ASENTAMIENTO DE PARTICULAS (26) SITUADA DENTRO DEL RECIPIENTE (10) Y AL MENOS PARCIALMENTE INMERSO EN EL MEDIO DE CULTIVO AGITADO EN SU INTERIOR. LA CAMARA DE ASENTAMIENTO DE PARTICULAS (26) COMPRENDE UN CONTENEDOR HUECO (27, 28) CON UNA ABERTURA INFERIOR (18) A TRAVES DE LA CUAL LAS PARTICULAS DE BIOMASA (15),TAL COMO CELULAS ENLAZADAS DE MICROPORTADORAS, SE ESTABLECEN POR UN REFUERZO DE GRAVEDAD DENTRO DEL MEDIO DE CULTIVO AGITADO Y UNA ABERTURA SUPERIOR (19) A TRAVES DE LA CUAL SE RETIRA EL MEDIO DE CULTIVO GASTADO LIBRE DE PARTICULAS DEL RECIPIENTE (10). LA CAMARA DE ASENTAMIENTO (26) ESTA CONFIGURADA DE MANERA QUE LA VELOCIDAD DE FLUIDO DE MEDIO DE CULTIVO SE INTRODUCE DENTRO DE LA CAMARA DE ASENTAMIENTO (26) A TRAVES DE LA ABERTURA INFERIOR (18) ES SIGNIFICANTEMENTE INFERIOR A LA VELOCIDAD DE ASENTAMIENTO DE PARTICULAS DE BIOMASA.
ES93903440T 1992-01-17 1993-01-15 Metodo y aparato para propagar particulas de biomasa. Expired - Lifetime ES2151901T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US82230192A 1992-01-17 1992-01-17

Publications (1)

Publication Number Publication Date
ES2151901T3 true ES2151901T3 (es) 2001-01-16

Family

ID=25235685

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93903440T Expired - Lifetime ES2151901T3 (es) 1992-01-17 1993-01-15 Metodo y aparato para propagar particulas de biomasa.

Country Status (15)

Country Link
US (1) US5654197A (es)
EP (1) EP0585419B1 (es)
JP (1) JP3244701B2 (es)
AT (1) ATE197813T1 (es)
AU (1) AU664596B2 (es)
CA (1) CA2106064C (es)
DE (1) DE69329706T2 (es)
DK (1) DK0585419T3 (es)
ES (1) ES2151901T3 (es)
GR (1) GR3035408T3 (es)
HK (1) HK1012674A1 (es)
IL (1) IL104385A (es)
PT (1) PT585419E (es)
WO (1) WO1993014192A1 (es)
ZA (1) ZA93310B (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5998184A (en) * 1997-10-08 1999-12-07 Unisyn Technologies, Inc. Basket-type bioreactor
US6335191B1 (en) 1998-02-27 2002-01-01 Nch Corporation Automated system and method for growing bacteria
DE19918409A1 (de) * 1999-04-22 2000-10-26 Forschungszentrum Juelich Gmbh Bioreaktor umfassend einen Medienablauf und einen Rührer sowie Rührer und Medienablauf
EP1325147A2 (en) * 2000-10-02 2003-07-09 Novo Nordisk A/S Industrial-scale serum-free production of recombinant factor vii in mammalian cells
US20040185535A1 (en) * 2003-03-21 2004-09-23 Giles Wilson Industrial-scale serum-free production of recombinant FVII in mammalian cells
US20040185534A1 (en) * 2000-10-02 2004-09-23 Knudsen Ida Molgaard Industrial-scale serum-free production of recombinant proteins in mammalian cells
WO2002087662A1 (en) * 2001-04-27 2002-11-07 Nexell Therapeutics Inc. Cell processing and fluid transfer apparatus and method of use
BR0212780A (pt) * 2001-10-02 2004-10-13 Novo Nordisk Healthcare Ag Métodos para produzir polipeptìdeos em células eucarióticas, para cultivar células eucarióticas e para colher polipeptìdeos produzidos por células eucarióticas
US7160719B2 (en) 2002-06-07 2007-01-09 Mayo Foundation For Medical Education And Research Bioartificial liver system
CN1300297C (zh) * 2002-09-20 2007-02-14 华东理工大学 一种用于动物细胞扩大接种的消化反应器
US7081361B2 (en) 2003-08-07 2006-07-25 Nch Corporation Biomass generator
US20080020049A1 (en) * 2005-02-25 2008-01-24 Andrew Darling Super-sparger microcarrier beads and precision extrusion deposited poly-epsilon-caprolactone structures for biological applications
US10130748B2 (en) * 2009-03-13 2018-11-20 Mayo Foundation For Medical Education And Research Bioartificial liver
US8551762B2 (en) 2009-07-07 2013-10-08 Nch Corporation System and apparatus for feeding, solubilizing, growing and discharging a biological material
US8961893B2 (en) * 2009-07-07 2015-02-24 Nch Corporation Automated chemical diluter system having disposable components
DE102009026366A1 (de) 2009-08-12 2011-02-17 Technische Universität München Fermentationsbehälter sowie Verfahren und Vorrichtung zur Fermentation
JP5415330B2 (ja) * 2010-03-12 2014-02-12 株式会社日立製作所 生体細胞の培養装置及び培養方法
ES2890412T3 (es) * 2011-01-17 2022-01-19 Hoffmann La Roche Aparato de separación
US20170096628A1 (en) * 2014-04-14 2017-04-06 Enevor Inc. Conical Impeller and Applications Thereof
US20170218330A1 (en) * 2016-02-01 2017-08-03 Fenwal, Inc. Systems and methods for the separation of cells from microcarriers using a spinning membrane
JP6744161B2 (ja) * 2016-07-19 2020-08-19 株式会社日立プラントサービス 分離装置、培養装置および分離方法
CN114058505B (zh) 2020-07-30 2024-10-01 环球生命科技咨询美国有限责任公司 新型灌流培养用高密度微载体截留装置和使用方法
JP7524841B2 (ja) * 2021-06-24 2024-07-30 横河電機株式会社 細胞培養プロセスに使用するための溶液処理デバイス

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR821326A (fr) * 1936-05-04 1937-12-02 Procédé et appareil pour la fermentation continue de solutions, en vue de l'obtention de produits de fermentation ou de micro-organismes qui se multiplient
US2451156A (en) * 1944-06-19 1948-10-12 Mattos Annibal Ramos De Process and apparatus for producing alcohol by fermentation
JPS575690A (en) * 1980-06-13 1982-01-12 Toray Ind Inc Circulation device for culture medium in cultivation tank
US4335215A (en) * 1980-08-27 1982-06-15 Monsanto Company Method of growing anchorage-dependent cells
CS231837B1 (en) * 1982-07-30 1984-12-14 Svatopluk Mackrle Device for a biological water purification
US4814278A (en) * 1985-02-05 1989-03-21 Teijin Limited Culture apparatus and method
JPS63123379A (ja) * 1986-11-12 1988-05-27 Teijin Ltd 動物細胞の培養方法
GB8515636D0 (en) * 1985-06-20 1985-07-24 Celltech Ltd Fermenter
JPH0195769A (ja) * 1987-10-08 1989-04-13 Ebara Res Co Ltd 高濃度培養装置
JPH0380077A (ja) * 1989-08-23 1991-04-04 Meiji Milk Prod Co Ltd マイクロキャリア分離方法及び装置
WO1991006627A1 (en) * 1989-11-03 1991-05-16 Massachusetts Institute Of Technology Nested-cone separator
JP3385741B2 (ja) * 1994-08-22 2003-03-10 日本ゼオン株式会社 医用材料組成物、その調製方法及び医用成形物

Also Published As

Publication number Publication date
PT585419E (pt) 2001-04-30
ZA93310B (en) 1993-09-08
DK0585419T3 (da) 2001-03-19
EP0585419A4 (en) 1995-04-05
EP0585419B1 (en) 2000-11-29
IL104385A0 (en) 1993-05-13
CA2106064C (en) 2005-09-20
JPH06510205A (ja) 1994-11-17
EP0585419A1 (en) 1994-03-09
AU664596B2 (en) 1995-11-23
WO1993014192A1 (en) 1993-07-22
AU3470393A (en) 1993-08-03
ATE197813T1 (de) 2000-12-15
JP3244701B2 (ja) 2002-01-07
IL104385A (en) 1995-12-31
HK1012674A1 (en) 1999-08-06
GR3035408T3 (en) 2001-05-31
DE69329706D1 (de) 2001-01-04
CA2106064A1 (en) 1993-07-18
US5654197A (en) 1997-08-05
DE69329706T2 (de) 2001-03-29

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