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ES2149672A1 - HUMAN GENE SEQUENCE HOMOLOGATES THE YEAST AFCI GENE INTRODUCED IN THE PROTEOLYTIC PROCESSING OF PRENYLATED PROTEINS. . - Google Patents

HUMAN GENE SEQUENCE HOMOLOGATES THE YEAST AFCI GENE INTRODUCED IN THE PROTEOLYTIC PROCESSING OF PRENYLATED PROTEINS. .

Info

Publication number
ES2149672A1
ES2149672A1 ES009800016A ES9800016A ES2149672A1 ES 2149672 A1 ES2149672 A1 ES 2149672A1 ES 009800016 A ES009800016 A ES 009800016A ES 9800016 A ES9800016 A ES 9800016A ES 2149672 A1 ES2149672 A1 ES 2149672A1
Authority
ES
Spain
Prior art keywords
proteolytic processing
yeast
homologates
afci
prenylated proteins
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
ES009800016A
Other languages
Spanish (es)
Other versions
ES2149672B1 (en
Inventor
Freije Jose Maria Perez
Cotarelo Gloria Velasco
Pendas Alberto Martin
Albors Pilar Blay
Felechosa Milagros Balbin
Otin Carlos Lopez
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universidad de Oviedo
Original Assignee
Universidad de Oviedo
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universidad de Oviedo filed Critical Universidad de Oviedo
Priority to ES9901608A priority Critical patent/ES2151459B1/en
Priority to ES9800016A priority patent/ES2149672B1/en
Priority to PCT/ES1999/000001 priority patent/WO1999035275A1/en
Publication of ES2149672A1 publication Critical patent/ES2149672A1/en
Application granted granted Critical
Publication of ES2149672B1 publication Critical patent/ES2149672B1/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489—Metalloendopeptidases (3.4.24)
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/574—Immunoassay; Biospecific binding assay; Materials therefor for cancer
    • G01N33/57484—Immunoassay; Biospecific binding assay; Materials therefor for cancer involving compounds serving as markers for tumor, cancer, neoplasia, e.g. cellular determinants, receptors, heat shock/stress proteins, A-protein, oligosaccharides, metabolites
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Human genetic sequences which are homologous to the yeast gene involved in the proteolytic processing of prenylated proteins. The invention comprises the identification of homologous fragments of human genes AFC1 and RCE1 of Saccharomyces cerevisiae, amplifying them through PCR of total RNA, using the amplified fragments as probes to hybridize cDNA and determining the sequence of the clones of cDNA which hybridize with the probes. The identified sequences are SEQ ID NO: 1 and SEQ ID NO: 2. The applications of said sequences concern essentially diagnosis and treatment of oncogenic troubles.
ES9800016A 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS. Expired - Fee Related ES2149672B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES9901608A ES2151459B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE RCE1 GENE OF YEAST INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.
ES9800016A ES2149672B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.
PCT/ES1999/000001 WO1999035275A1 (en) 1998-01-08 1999-01-08 Human genetic sequences which are homologous to the yeast genes involved in the proteolytic processing of prenylated proteins

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES9800016A ES2149672B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.

Publications (2)

Publication Number Publication Date
ES2149672A1 true ES2149672A1 (en) 2000-11-01
ES2149672B1 ES2149672B1 (en) 2001-05-16

Family

ID=8302331

Family Applications (2)

Application Number Title Priority Date Filing Date
ES9800016A Expired - Fee Related ES2149672B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE AFC1 GENE OF LEAVES INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.
ES9901608A Expired - Fee Related ES2151459B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE RCE1 GENE OF YEAST INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES9901608A Expired - Fee Related ES2151459B1 (en) 1998-01-08 1998-01-08 HUMAN GENE SEQUENCE APPROVES THE RCE1 GENE OF YEAST INVOLVED IN THE PROTEOLITIC PROCESSING OF PRENILED PROTEINS.

Country Status (2)

Country Link
ES (2) ES2149672B1 (en)
WO (1) WO1999035275A1 (en)

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002509428A (en) * 1996-08-07 2002-03-26 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア AFC1 and RCE1: isoprenylated CAAX processing enzyme
WO1998054333A2 (en) * 1997-06-02 1998-12-03 Acacia Biosciences Inc. Mammalian caax processing enzymes
CA2234418A1 (en) * 1997-06-24 1998-12-24 Smithkline Beecham Corporation Human afc1
CA2234402A1 (en) * 1997-06-24 1998-12-24 Smithkline Beecham Corporation Human rce1

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
ASHBY, M.N. et al. "Ras and a-Factor converting enzyme", METHODS IN ENZYMOLOGY, 1995, Vol. 250, paginas 235-251. *
BASSET, D.E. et al. "Genome cross-referencing and XREF db: Implications for the identification and analysis of genes mutated in human disease", NATURE GENETICS, 1997, Vol. 15, paginas 339-344. *
BOYARTCHUK, V.L. et al. "Modulation of Ras and a-Factor function by carboxylterminal proteolysis", SCIENCE, 1997, Vol. 275, paginas 1796-1800. *
FUJIMURA-KAMADA, K. et al. "A novel membrane-associated metalloprotease, Step 24, is required for the first step of NH2-terminal processing of the yeast a-Factor precursor", THE JOURNAL OF CELL BIOLOGY, 1997, Vol. 136, No 2, paginas 271-285. *

Also Published As

Publication number Publication date
WO1999035275A1 (en) 1999-07-15
ES2149672B1 (en) 2001-05-16
ES2151459B1 (en) 2001-07-01
ES2151459A1 (en) 2000-12-16

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