ES2095322T3 - Procedimiento para aislar mutantes de delecion vibrio cholerae y cultivos que contienen vibrio cholerae. - Google Patents
Procedimiento para aislar mutantes de delecion vibrio cholerae y cultivos que contienen vibrio cholerae.Info
- Publication number
- ES2095322T3 ES2095322T3 ES91912336T ES91912336T ES2095322T3 ES 2095322 T3 ES2095322 T3 ES 2095322T3 ES 91912336 T ES91912336 T ES 91912336T ES 91912336 T ES91912336 T ES 91912336T ES 2095322 T3 ES2095322 T3 ES 2095322T3
- Authority
- ES
- Spain
- Prior art keywords
- vibrio cholerae
- deletion
- chromosome
- vivo recombination
- isolating
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000037430 deletion Effects 0.000 title abstract 8
- 238000012217 deletion Methods 0.000 title abstract 8
- 241000607626 Vibrio cholerae Species 0.000 title abstract 6
- 229940118696 vibrio cholerae Drugs 0.000 title abstract 6
- 238000000034 method Methods 0.000 title abstract 4
- 210000000349 chromosome Anatomy 0.000 abstract 4
- 238000001727 in vivo Methods 0.000 abstract 3
- 238000005215 recombination Methods 0.000 abstract 3
- 230000006798 recombination Effects 0.000 abstract 3
- 239000013612 plasmid Substances 0.000 abstract 2
- 108090000623 proteins and genes Proteins 0.000 abstract 2
- 108091008146 restriction endonucleases Proteins 0.000 abstract 2
- 238000012512 characterization method Methods 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 101150065227 ctx gene Proteins 0.000 abstract 1
- 230000029087 digestion Effects 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 239000003550 marker Substances 0.000 abstract 1
- 230000035772 mutation Effects 0.000 abstract 1
- 210000001578 tight junction Anatomy 0.000 abstract 1
- 239000003053 toxin Substances 0.000 abstract 1
- 231100000765 toxin Toxicity 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/28—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Vibrionaceae (F)
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Gastroenterology & Hepatology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Enzymes And Modification Thereof (AREA)
- Dairy Products (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
ESTA INVENCION SE REFIERE A METODOS PARA AISLAR LOS MUTANTES DE ANULACION DE VIBRIO CHOLERAE. EN UN METODO, LA ANULACION ES PREDETERMINADA POR LA DIGESTION CON ENDONUCLEASAS DE RESTRICCION DE ESPECIFICIDAD CONOCIDA. LAS ANULACIONES SE INSERTAN EN EL CROMOSOMA VIBRIO CHOLERAE MEDIANTE UNA RECOMBINACION IN VIVO ENTRE UN PLASMIDO QUE LLEVA LA ANULACION DESEADA, CON SECUENCIAS DE FLANQUEO ADYACENTES Y EL CROMOSOMA VIBRIO CHOLERAE. EN OTRO METODO, UN EVENTO DE RECOMBINACION IN VIVO INICIAL DE SECUENCIAS HOMOLOGAS DEL PLASMIDO RECOMBINANTE EN EL CROMOSOMA PROPORCIONA UN MARCADOR SELECCIONABLE EN ESTE LUGAR. UN SEGUNDO EVENTO DE RECOMBINACION IN VIVO ENTRE SECUENCIAS DE FLANQUEO HOMOLOGAS DA COMO RESULTADO UNA EXCISION DE LOS GENES COMPETENTES DEL CROMOSOMA CON EL PRODUCTO FINAL SIENDO UNA MUTACION DE ANULACION. LA INVENCION INCLUYE EL AISLAMIENTO Y CARACTERIZACION DE UNA NUEVA CADENA DE VIBRIO CHOLERAE QUE TIENE UNA ANULACION EN EL GEN CTX, COMO QUEDA DEFINIDO POR ACC I, XBA I, CLA I Y/O LUGARESDE ENDONUCLEASA DE RESTRICCION Y QUE ADEMAS TIENE UNA ANULACION EN EL GEN QUE CODIFICA LA TOXINA ZONULA OCCLUDENS.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US53331590A | 1990-06-05 | 1990-06-05 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2095322T3 true ES2095322T3 (es) | 1997-02-16 |
Family
ID=24125419
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES91912336T Expired - Lifetime ES2095322T3 (es) | 1990-06-05 | 1991-06-05 | Procedimiento para aislar mutantes de delecion vibrio cholerae y cultivos que contienen vibrio cholerae. |
Country Status (13)
| Country | Link |
|---|---|
| EP (1) | EP0485591B1 (es) |
| JP (1) | JP3600618B2 (es) |
| AT (1) | ATE145005T1 (es) |
| AU (1) | AU656730B2 (es) |
| CA (1) | CA2064046C (es) |
| DE (1) | DE69123027T2 (es) |
| DK (1) | DK0485591T3 (es) |
| ES (1) | ES2095322T3 (es) |
| GR (1) | GR3022127T3 (es) |
| RU (1) | RU2130970C1 (es) |
| WO (1) | WO1991018979A1 (es) |
| YU (1) | YU141191A (es) |
| ZA (1) | ZA914286B (es) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5882653A (en) * | 1983-03-04 | 1999-03-16 | The University Of Maryland System | Vibrio cholerae 01 (CVD111) and non-01 (CVD112 and CVD112RM) serogroup vaccine strains, methods of making same and products thereof |
| US5874088A (en) * | 1992-07-06 | 1999-02-23 | President And Fellows Of Harvard College | Deletion mutants of cholera vaccines expressing heterologous antigens |
| CN1083524A (zh) * | 1992-07-06 | 1994-03-09 | 哈佛大学校长及研究员协会 | 作为霍乱疫苗缺失突变体 |
| US6203799B1 (en) | 1993-07-01 | 2001-03-20 | Presidents And Fellows Of Harvard College | Vibrio cholerae mutants which are soft-agar penetration defective and lack a functional CtxA subunit |
| JP3379582B2 (ja) * | 1993-10-08 | 2003-02-24 | ユニバーシティ・オブ・メリーランド・アット・ボルティモア | Ｖｉｂｒｉｏ ｃｈｏｌｅｒａｅ ０１（ｃｖｄ１１１）および−非０１（ｃｖｄ１１２およびｃｖｄ１１２ｒｍ）血清群ワクチン株、その作製方法、およびその産物 |
| EP0928831B1 (en) * | 1997-12-10 | 2006-05-03 | Council of Scientific and Industrial Research | Cholera organisms, vaccines and preparation thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5135862A (en) * | 1983-03-04 | 1992-08-04 | University Of Maryland | Method of isolating restriction fragment deletions in vibrio cholerae, products thereof |
| US4882278A (en) * | 1983-04-29 | 1989-11-21 | President And Fellows Of Harvard College | Non-toxinogenic vibrio cholerae mutants |
-
1991
- 1991-06-05 CA CA002064046A patent/CA2064046C/en not_active Expired - Fee Related
- 1991-06-05 AT AT91912336T patent/ATE145005T1/de not_active IP Right Cessation
- 1991-06-05 JP JP51173791A patent/JP3600618B2/ja not_active Expired - Fee Related
- 1991-06-05 YU YU141191A patent/YU141191A/sh unknown
- 1991-06-05 DE DE69123027T patent/DE69123027T2/de not_active Expired - Fee Related
- 1991-06-05 ES ES91912336T patent/ES2095322T3/es not_active Expired - Lifetime
- 1991-06-05 EP EP91912336A patent/EP0485591B1/en not_active Expired - Lifetime
- 1991-06-05 DK DK91912336.4T patent/DK0485591T3/da active
- 1991-06-05 WO PCT/US1991/003812 patent/WO1991018979A1/en active IP Right Grant
- 1991-06-05 RU SU5011181A patent/RU2130970C1/ru not_active IP Right Cessation
- 1991-06-05 ZA ZA914286A patent/ZA914286B/xx unknown
- 1991-06-05 AU AU82147/91A patent/AU656730B2/en not_active Ceased
-
1996
- 1996-12-20 GR GR960403562T patent/GR3022127T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP3600618B2 (ja) | 2004-12-15 |
| EP0485591A4 (en) | 1993-07-14 |
| ATE145005T1 (de) | 1996-11-15 |
| AU8214791A (en) | 1991-12-31 |
| CA2064046A1 (en) | 1991-12-06 |
| AU656730B2 (en) | 1995-02-16 |
| DE69123027D1 (de) | 1996-12-12 |
| YU141191A (sh) | 1994-06-24 |
| DE69123027T2 (de) | 1997-03-13 |
| EP0485591B1 (en) | 1996-11-06 |
| CA2064046C (en) | 1999-04-13 |
| JPH05502381A (ja) | 1993-04-28 |
| WO1991018979A1 (en) | 1991-12-12 |
| ZA914286B (en) | 1992-03-25 |
| GR3022127T3 (en) | 1997-03-31 |
| RU2130970C1 (ru) | 1999-05-27 |
| EP0485591A1 (en) | 1992-05-20 |
| DK0485591T3 (da) | 1997-04-07 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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