ES2078174A1 - Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19 - Google Patents
Molecular diagnostic methods for the detection of Brucella abortus and differentiation from vaccine strain B19Info
- Publication number
- ES2078174A1 ES2078174A1 ES09302728A ES9302728A ES2078174A1 ES 2078174 A1 ES2078174 A1 ES 2078174A1 ES 09302728 A ES09302728 A ES 09302728A ES 9302728 A ES9302728 A ES 9302728A ES 2078174 A1 ES2078174 A1 ES 2078174A1
- Authority
- ES
- Spain
- Prior art keywords
- cvabrucella
- differentiation
- methods
- molecular diagnostic
- diagnostic methods
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000002405 diagnostic procedure Methods 0.000 title abstract 3
- 230000004069 differentiation Effects 0.000 title abstract 3
- 238000001514 detection method Methods 0.000 title abstract 2
- 229960005486 vaccine Drugs 0.000 title abstract 2
- 241000589567 Brucella abortus Species 0.000 title 1
- 229940056450 brucella abortus Drugs 0.000 title 1
- 238000000034 method Methods 0.000 abstract 5
- 108091034117 Oligonucleotide Proteins 0.000 abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 abstract 2
- 238000009396 hybridization Methods 0.000 abstract 2
- UNXHWFMMPAWVPI-UHFFFAOYSA-N Erythritol Natural products OCC(O)C(O)CO UNXHWFMMPAWVPI-UHFFFAOYSA-N 0.000 abstract 1
- 239000004386 Erythritol Substances 0.000 abstract 1
- 239000012472 biological sample Substances 0.000 abstract 1
- 238000012136 culture method Methods 0.000 abstract 1
- UNXHWFMMPAWVPI-ZXZARUISSA-N erythritol Chemical compound OC[C@H](O)[C@H](O)CO UNXHWFMMPAWVPI-ZXZARUISSA-N 0.000 abstract 1
- 229940009714 erythritol Drugs 0.000 abstract 1
- 235000019414 erythritol Nutrition 0.000 abstract 1
- 238000013412 genome amplification Methods 0.000 abstract 1
- 230000001900 immune effect Effects 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 239000007858 starting material Substances 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
METODOS DE DIAGNOSTICO MOLECULAR PARA LA DETECCION DE CVABRUCELLA ABORTUS Y SU DIFERENCIACION DE LA CEPA VACUNAL B19. SE DESCRIBEN UNA SERIE DE METODOS DE DIAGNOSTICO MOLECULAR QUE PERMITEN A DIFERENCIACION ENTRE LA ESTIRPE VACUNAL CVABRUCELLA ABORTUS B19 Y TODAS LAS DEMAS ESTIRPES DEL GENERO CVABRUCELLA. EN UN CASO SE APLICA LA AMPLIFICACION DE GENOMA, USANDO COMO INICIADORES OLIGONUCLEOTIDOS DERIVADOS DE LA REGION QUE CONTIENE LOS GENES CODIFICANTES DE LA RUTA DE UTILIZACION DEL ERITRITOL. OTRO GRUPO DE METODOS SE BASA EN LA HIBRIDACION DE ADN GENOMICO USANDO COMO SONDAS OLIGONUCLEOTIDOS DE LA MENCIONADA REGION CVAERI. EN ESTE CASO PUEDEN APLICARSE SEGUN LA TECNICA DENOMINADA "HIBRIDACION TIPO SOUTHERN" O ALTERNATIVAMENTE POR EL PROCEDIMIENTO DEL "DOT BLOT". FINALMENTE SE APLICAN ESTOS METODOS PARA LA IDENTIFICACION DE GERMENES DEL GENERO CVABRUCELLA EN CUALQUIER MUESTRA BIOLOGICA. LOS NUEVOS PROCEDIMIENTOS SON VENTAJOSOS SOBRE LOS METODOS TRADICIONALES, INMUNOLOGICOS O DE CULTIVO, APORTANDO MAYOR ESPECIFICIDAD Y RAPIDEZ.MOLECULAR DIAGNOSTIC METHODS FOR THE DETECTION OF CVABRUCELLA ABORTUS AND ITS DIFFERENTIATION FROM THE B19 VACCINAL STRAIN. A SERIES OF MOLECULAR DIAGNOSTIC METHODS ARE DESCRIBED THAT ALLOW THE DIFFERENTIATION BETWEEN THE CVABRUCELLA ABORTUS B19 VACCINE STRAIN AND ALL OTHER STRAINS OF THE CVABRUCELLA GENUS. IN ONE CASE, GENOME AMPLIFICATION IS APPLIED, USING OLIGONUCLEOTIDES AS STARTERS DERIVED FROM THE REGION CONTAINING THE GENES CODING THE ROUTE OF USE OF ERYTHRITOL. ANOTHER GROUP OF METHODS IS BASED ON THE HYBRIDIZATION OF GENOMIC DNA USING OLIGONUCLEOTIDES FROM THE MENTIONED CVAERI REGION AS PROBE. IN THIS CASE THEY MAY BE APPLIED ACCORDING TO THE TECHNIQUE CALLED "SOUTHERN TYPE HYBRIDIZATION" OR ALTERNATIVELY BY THE "DOT BLOT" PROCEDURE. FINALLY, THESE METHODS ARE APPLIED FOR THE IDENTIFICATION OF GERMS OF THE CVABRUCELLA GENUS IN ANY BIOLOGICAL SAMPLE. THE NEW PROCEDURES ARE ADVANTAGEOUS OVER TRADITIONAL, IMMUNOLOGICAL OR CULTURE METHODS, PROVIDING GREATER SPECIFICITY AND RAPIDITY.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09302728A ES2078174B1 (en) | 1993-12-16 | 1993-12-16 | MOLECULAR DIAGNOSTIC METHOD FOR THE DETECTION OF BRUCELLA ABORTUS AND DIFFERENTIATION OF VACCINE STRAIN B19. |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| ES09302728A ES2078174B1 (en) | 1993-12-16 | 1993-12-16 | MOLECULAR DIAGNOSTIC METHOD FOR THE DETECTION OF BRUCELLA ABORTUS AND DIFFERENTIATION OF VACCINE STRAIN B19. |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| ES2078174A1 true ES2078174A1 (en) | 1995-12-01 |
| ES2078174B1 ES2078174B1 (en) | 1996-08-16 |
Family
ID=8284078
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES09302728A Expired - Fee Related ES2078174B1 (en) | 1993-12-16 | 1993-12-16 | MOLECULAR DIAGNOSTIC METHOD FOR THE DETECTION OF BRUCELLA ABORTUS AND DIFFERENTIATION OF VACCINE STRAIN B19. |
Country Status (1)
| Country | Link |
|---|---|
| ES (1) | ES2078174B1 (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006097347A2 (en) * | 2005-03-17 | 2006-09-21 | Instituto Científico Y Tecnológico De Navarra, S.A. | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
-
1993
- 1993-12-16 ES ES09302728A patent/ES2078174B1/en not_active Expired - Fee Related
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4358535A (en) * | 1980-12-08 | 1982-11-09 | Board Of Regents Of The University Of Washington | Specific DNA probes in diagnostic microbiology |
| US4358535B1 (en) * | 1980-12-08 | 1986-05-13 | ||
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US4683202B1 (en) * | 1985-03-28 | 1990-11-27 | Cetus Corp |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2006097347A2 (en) * | 2005-03-17 | 2006-09-21 | Instituto Científico Y Tecnológico De Navarra, S.A. | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
| WO2006097347A3 (en) * | 2005-03-17 | 2007-01-04 | Inst Cientifico Tecnol Navarra | Methods for detecting, identifying and differentiating species and vaccine strains of the brucella genus |
Also Published As
| Publication number | Publication date |
|---|---|
| ES2078174B1 (en) | 1996-08-16 |
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