ES2073409T3 - Dna conteniendo el codigo de la sintetasa del acv. - Google Patents
Dna conteniendo el codigo de la sintetasa del acv.Info
- Publication number
- ES2073409T3 ES2073409T3 ES88311655T ES88311655T ES2073409T3 ES 2073409 T3 ES2073409 T3 ES 2073409T3 ES 88311655 T ES88311655 T ES 88311655T ES 88311655 T ES88311655 T ES 88311655T ES 2073409 T3 ES2073409 T3 ES 2073409T3
- Authority
- ES
- Spain
- Prior art keywords
- acv
- penicillin
- gene
- hosts
- expression
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/93—Ligases (6)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
Abstract
Un procedimiento para la transformación de una célula huésped con un vector recombinante, el cual, comprende el mezclado conjunto del huésped y del vector recombinante bajo condiciones de transformación convencionales, caracterizado porque el vector recombinante comprende DNA, el cual, comprende, esencialmente, un gen que contiene el código de una enzima sintetasa capaz de generar (L-aminoadipil)-L-cisteinil-D-valina obtenible a partir de Penicillium chrysogenum, mediante: a) la construcción de una biblioteca genómica a partir de los fragmentos de DNA cromosómicos del P. chrysogenum, b) la realización de uno o más experimentos de hibridación con el fin de seleccionar, a partir de dicha biblioteca, los clones que contengan el DNA de la invención y c) el aislamiento del DNA de la invención, conteniendo dicho DNA la configuración de los sitios de restricción mostrados en la Figura 6(b).
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB878728811A GB8728811D0 (en) | 1987-12-09 | 1987-12-09 | Novel substance |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2073409T3 true ES2073409T3 (es) | 1995-08-16 |
Family
ID=10628254
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES88311655T Expired - Lifetime ES2073409T3 (es) | 1987-12-09 | 1988-12-09 | Dna conteniendo el codigo de la sintetasa del acv. |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP0320272B1 (es) |
| JP (1) | JP2970918B2 (es) |
| AT (1) | ATE122718T1 (es) |
| DE (1) | DE3853811T2 (es) |
| ES (1) | ES2073409T3 (es) |
| GB (1) | GB8728811D0 (es) |
| GR (1) | GR3017073T3 (es) |
| WO (1) | WO1989005350A1 (es) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5108918A (en) * | 1988-08-11 | 1992-04-28 | Gist-Brocades | Method for identifying and using biosynthetic genes for enhanced production of secondary metabolites |
| FI104264B (fi) * | 1988-08-11 | 1999-12-15 | Dsm Ip Assets Bv | Menetelmä sekundääristen metaboliittien tuoton parantamiseksi käyttämällä rykelmöityjä biosynteettisiä geenejä |
| EP0444758A1 (en) * | 1990-02-28 | 1991-09-04 | Gist-Brocades N.V. | Method for modification of ACV synthetase and use of the modified enzyme for production of beta-lactam antibiotics |
| US5882879A (en) * | 1990-02-28 | 1999-03-16 | Gist-Brocades, N.V. | Method for influencing β-lactam antibiotic production and for isolation of large quantities of ACV synthetase |
| US6322998B1 (en) | 1990-06-01 | 2001-11-27 | Eli Lilly And Company | Recombinant DNA compounds that encode ACV synthetase activity of Cephalosporium acremonium |
| US5589571A (en) * | 1994-10-28 | 1996-12-31 | Cor Therapeutics, Inc. | Process for production of inhibited forms of activated blood factors |
| KR20070085955A (ko) * | 2004-12-10 | 2007-08-27 | 디에스엠 아이피 어셋츠 비.브이. | 단일 세포에서의 β-락탐의 제조 |
| WO2007147827A2 (en) * | 2006-06-22 | 2007-12-27 | Dsm Ip Assets B.V. | Production of pravastatin |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS59111699A (ja) * | 1982-12-17 | 1984-06-27 | 富士通株式会社 | 話者認識方式 |
| DE3683080D1 (de) * | 1985-04-22 | 1992-02-06 | Lilly Co Eli | Rekombinante dns-expressionsvektoren und dns-verbindungen. |
| US4892819A (en) * | 1985-11-25 | 1990-01-09 | Eli Lilly And Company | Recombinant DNA expression vectors and DNA compounds that encode isopenicillin N synthetase from penicillium chrysogenum |
| EP0233715B1 (en) * | 1986-01-31 | 1994-05-25 | BEECHAM GROUP plc | Isolation and expression of genes involved in the biosynthesis of beta-lactams |
| CA1327170C (en) * | 1987-03-04 | 1994-02-22 | Stephen Wyatt Queener | Recombinant dna expression vectors and dna compounds that encode deacetoxycephalosporin c synthetase and deacetylcephalosporin c synthetase |
| GB2208077A (en) * | 1987-04-24 | 1989-02-22 | Antibioticos Sa | Isopenicillin n synthetase gene derived from aspergillus nidulans |
-
1987
- 1987-12-09 GB GB878728811A patent/GB8728811D0/en active Pending
-
1988
- 1988-12-09 DE DE3853811T patent/DE3853811T2/de not_active Expired - Lifetime
- 1988-12-09 ES ES88311655T patent/ES2073409T3/es not_active Expired - Lifetime
- 1988-12-09 JP JP1500399A patent/JP2970918B2/ja not_active Expired - Lifetime
- 1988-12-09 AT AT88311655T patent/ATE122718T1/de not_active IP Right Cessation
- 1988-12-09 WO PCT/GB1988/001083 patent/WO1989005350A1/en unknown
- 1988-12-09 EP EP88311655A patent/EP0320272B1/en not_active Expired - Lifetime
-
1995
- 1995-08-09 GR GR950402201T patent/GR3017073T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0320272B1 (en) | 1995-05-17 |
| EP0320272A1 (en) | 1989-06-14 |
| WO1989005350A1 (en) | 1989-06-15 |
| GB8728811D0 (en) | 1988-01-27 |
| JPH02502606A (ja) | 1990-08-23 |
| GR3017073T3 (en) | 1995-11-30 |
| ATE122718T1 (de) | 1995-06-15 |
| DE3853811D1 (de) | 1995-06-22 |
| DE3853811T2 (de) | 1995-10-12 |
| JP2970918B2 (ja) | 1999-11-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FG2A | Definitive protection |
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