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ES2018901A6 - Metodo para producir y purificar ubiquitin-hidrolasa, asi como para disociar conjugados de ubiquitina-polipeptido. - Google Patents

Metodo para producir y purificar ubiquitin-hidrolasa, asi como para disociar conjugados de ubiquitina-polipeptido.

Info

Publication number
ES2018901A6
ES2018901A6 ES8902205A ES8902205A ES2018901A6 ES 2018901 A6 ES2018901 A6 ES 2018901A6 ES 8902205 A ES8902205 A ES 8902205A ES 8902205 A ES8902205 A ES 8902205A ES 2018901 A6 ES2018901 A6 ES 2018901A6
Authority
ES
Spain
Prior art keywords
dna sequences
sequences encoding
ubiquitin hydrolase
encoding same
ubiquitin
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
ES8902205A
Other languages
English (en)
Inventor
Chung-Cheng Liu
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Genentech Inc
Original Assignee
Genentech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/210,909 external-priority patent/US5156968A/en
Application filed by Genentech Inc filed Critical Genentech Inc
Publication of ES2018901A6 publication Critical patent/ES2018901A6/es
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/64—Relaxins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/58—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi
    • C12N9/60—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from fungi from yeast
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/95—Fusion polypeptide containing a motif/fusion for degradation (ubiquitin fusions, PEST sequence)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Endocrinology (AREA)
  • Microbiology (AREA)
  • Toxicology (AREA)
  • General Engineering & Computer Science (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

METODO PARA PRODUCIR Y PURIFICAR UBIQUITIN-HIDROLASA, ASI COMO PARA DISOCIAR CONJUGADOS DE UBIQUITINA-POLIPEPTIDO. SE TRANSFORMAN CELULAS HOSPEDANTES CON VECTORES DE EXPRESION QUE CODIFICAN UBIQUITIN-HIDROLASA, Y SE CULTIVAN DICHAS CELULAS PARA EXPRESAR HIDROLASA. SE HOMOGENEIZAN PASTAS DE CELULAS EUCARIOTICAS, RECUPERANDO LA PORCION CON ACTIVIDAD DE UBIQUITIN-HIDROLASA, SE SEPARA EL PRECIPITADO Y SE PONE EN CONTACTO CON RESINA INTERCAMBIADORA IONICA, Y DESPUES CON RESINA DE AFINIDAD HIDROFOBA, RECUPERANDO LA FRACCION ABSORBIDA A LA RESINA, Y SEGUIDAMENTE CON RESINA DE CROMATOGRAFIA, RECUPERANDO LA FRACCION ABSORBIDA A ESTA RESINA, Y NUEVAMENTE CON RESINA INTERCAMBIADORA IONICA, RECUPERANDO LA FRACCION CON ACTIVIDAD DE HIDROLASA Y ASILANDO IBIQUITIN-HIDROLASA. ESTA ES UTIL PARA DISOCIAR POLIPEPTIDOS DE FUSION DE UBIQUITINA-POLIPEPTIDO, RECUPERANDO LOS POLIPEPTIDOS LIBRES CORRESPONDIENTES.
ES8902205A 1988-06-24 1989-06-23 Metodo para producir y purificar ubiquitin-hidrolasa, asi como para disociar conjugados de ubiquitina-polipeptido. Expired - Fee Related ES2018901A6 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/210,909 US5156968A (en) 1988-06-24 1988-06-24 Purified yeast ubiquitin hydrolase
US07/284,281 US5108919A (en) 1988-06-24 1988-12-14 Dna sequences encoding yeast ubiquitin hydrolase

Publications (1)

Publication Number Publication Date
ES2018901A6 true ES2018901A6 (es) 1991-05-16

Family

ID=26905634

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8902205A Expired - Fee Related ES2018901A6 (es) 1988-06-24 1989-06-23 Metodo para producir y purificar ubiquitin-hidrolasa, asi como para disociar conjugados de ubiquitina-polipeptido.

Country Status (9)

Country Link
US (1) US5108919A (es)
EP (1) EP0420936B1 (es)
JP (1) JPH03505527A (es)
AU (1) AU619319B2 (es)
DE (1) DE68914664T2 (es)
DK (1) DK303790A (es)
ES (1) ES2018901A6 (es)
FI (1) FI906214A0 (es)
WO (1) WO1989012678A1 (es)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5093242A (en) * 1986-10-02 1992-03-03 Massachusetts Institute Of Technology Methods of generating desired amino-terminal residues in proteins
US5196321A (en) * 1986-10-02 1993-03-23 Massachusetts Institute Of Technology Methods for in vitro cleavage of ubiquitin fusion proteins
AU5963290A (en) * 1989-06-30 1991-01-17 Massachusetts Institute Of Technology Inhibition of the n-end rule pathway in living cells
US5620923A (en) * 1989-10-12 1997-04-15 The University Of Utah Synthesis of peptides as cloned ubiquitin extensions
EP0445097B1 (en) * 1990-02-26 1996-08-21 Washington University Method for protein N-myristoylation
ATE121454T1 (de) * 1990-05-09 1995-05-15 Massachusetts Inst Technology Ubiquitinspezifische protease.
US5212058A (en) * 1990-05-09 1993-05-18 Massachusetts Institute Of Technology Nucleic acid encoding ubiquitin-specific proteases
US5716816A (en) * 1992-05-22 1998-02-10 The United States Of America As Represented By The Department Of Health And Human Services Clones encoding mammalian ADP-ribosylarginine hydrolases
JPH08504763A (ja) * 1992-10-29 1996-05-21 セルトリックス ファーマシューティカルズ，インコーポレイテッド 治療薬としてのＴＧＦ−βレセプターフラグメントの使用
US6653450B1 (en) 1993-01-28 2003-11-25 Cohesion Technologies, Inc. Mutated recombinant collagens
US5459051A (en) * 1993-03-26 1995-10-17 Celtrix Pharmaceuticals, Inc. Methods and vectors for over-expression of ubiquitin fusion proteins in host cells
US5914254A (en) * 1993-08-02 1999-06-22 Celtrix Pharmaceuticals, Inc. Expression of fusion polypeptides transported out of the cytoplasm without leader sequences
US5563046A (en) * 1993-08-02 1996-10-08 Celtrix Pharmaceuticals, Inc. Fusion polypeptides and proteins
US5981699A (en) * 1994-01-04 1999-11-09 Mitotix, Inc. Human ubiquitin conjugating enzyme
US5744343A (en) * 1994-01-04 1998-04-28 Mitotix, Inc. Ubiquitin conjugating enzymes
ATE193123T1 (de) * 1994-01-04 2000-06-15 Mitotix Inc Ubiquitin-konjugierende enzyme
US5770720A (en) * 1995-08-30 1998-06-23 Barnes-Jewish Hospital Ubiquitin conjugating enzymes having transcriptional repressor activity
US6001619A (en) * 1995-10-04 1999-12-14 Cold Spring Harbor Laboratory Ubiquitin ligases, and uses related thereto
US6747128B2 (en) 1997-08-20 2004-06-08 Gpc Biotech, Inc. Components of ubiquitin ligase complexes, and uses related thereto
CN1297053A (zh) * 1999-11-22 2001-05-30 上海博容基因开发有限公司 一种新的多肽——人遍在蛋白c端水解酶28和编码这种多肽的多核苷酸
AU2001267181A1 (en) * 2000-05-30 2001-12-11 Viridis Biotech Inc. Polyubiquitin based hydrogel and uses thereof
US20050032683A1 (en) * 2000-10-04 2005-02-10 Amento Edward P. Methods of modulating apoptosis by administration of relaxin agonists or antagonists
PL205888B1 (pl) 2001-07-06 2010-06-30 Lg Life Sciences Ltd Aminopeptydaza, gen kodujący aminopeptydazę, wektor ekspresyjny, transformant Escherichia coli i sposób wytwarzania białka typu naturalnego
JP4088584B2 (ja) * 2001-07-26 2008-05-21 アドバンスド プロテイン テクノロジーズ コーポレイション 融合タンパク質から目的タンパク質を分離する方法。
EP1456349A4 (en) * 2001-12-12 2005-01-05 Lilly Co Eli EXPRESSION SYSTEM
NZ555206A (en) * 2004-12-22 2010-09-30 Ambrx Inc Methods for expression and purification of recombinant human growth hormone
US8093364B2 (en) 2008-01-18 2012-01-10 Bio-Rad Laboratories, Inc. Enhanced purification of antibodies and antibody fragments by apatite chromatography

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4757013A (en) * 1983-07-25 1988-07-12 The Research Foundation Of State University Of New York Cloning vehicles for polypeptide expression in microbial hosts
US4703004A (en) * 1984-01-24 1987-10-27 Immunex Corporation Synthesis of protein with an identification peptide
US4771002A (en) * 1984-02-24 1988-09-13 Lubrizol Genetics, Inc. Transcription in plants and bacteria
EP0324789B1 (en) * 1986-10-02 2003-08-20 Massachusetts Institute Of Technology Methods of regulating metabolic stability of proteins
JPH02500876A (ja) * 1987-03-20 1990-03-29 クリエイティブ・バイオマリキュールズ・インコーポレーテッド 組換えポリペプチドの製品およびその製造、単離および精製方法

Also Published As

Publication number Publication date
DE68914664T2 (de) 1994-10-20
DE68914664D1 (de) 1994-05-19
AU3865989A (en) 1990-01-12
JPH03505527A (ja) 1991-12-05
FI906214A0 (fi) 1990-12-17
EP0420936A1 (en) 1991-04-10
WO1989012678A1 (en) 1989-12-28
US5108919A (en) 1992-04-28
DK303790D0 (da) 1990-12-21
DK303790A (da) 1991-02-22
EP0420936B1 (en) 1994-04-13
AU619319B2 (en) 1992-01-23

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FD1A Patent lapsed

Effective date: 20100315