ES2012530A6 - Metodo de produccion de un edulcorante proteinico. - Google Patents
Metodo de produccion de un edulcorante proteinico.Info
- Publication number
- ES2012530A6 ES2012530A6 ES8801903A ES8801903A ES2012530A6 ES 2012530 A6 ES2012530 A6 ES 2012530A6 ES 8801903 A ES8801903 A ES 8801903A ES 8801903 A ES8801903 A ES 8801903A ES 2012530 A6 ES2012530 A6 ES 2012530A6
- Authority
- ES
- Spain
- Prior art keywords
- preparation
- novel protein
- sweeteners
- protein sweeteners
- novel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 235000003599 food sweetener Nutrition 0.000 title abstract 3
- 239000003765 sweetening agent Substances 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 108050004114 Monellin Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 238000010188 recombinant method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
- C07K14/43—Sweetening agents, e.g. thaumatin, monellin
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Botany (AREA)
- Plant Pathology (AREA)
- Gastroenterology & Hepatology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Seasonings (AREA)
Abstract
UN METODO DE PRODUCCION DE UN EDULCORANTE PROTEINICO QUE PRESENTA UNA HOMOLOGIA DE ALREDEDOR DEL 80% COMO MINIMO CON LAS DOS SUB-UNIDADES DE LA MONELINA QUE COMPRENDE (A) CRECER EN UN MEDIO NUTRITIVO UN HUESPED MICROBIANO PORTADOR DE UN MODULO DE EXPRESION QUE CONTIENE EN LA DIRECCION DE TRANSCRIPCION, UNA REGION REGULADORA DEL INICIO DE LA TRANSCRIPCION Y DE LA TRADUCCION, UNA SECUENCIA DE ADN QUE CODIFICA UN POLIPEPTIDO QUE COMPRENDE UNA SECUENCIA DE AMINOACIDOS QUE CONTIENE AL MENOS EL 80% DE LOS AMINOACIDOS DE LA MONELINA Y UNA REGION DE TERMINACION DE LA TRANSCRIPCION Y DE LA TRADUCCION Y (B) AISLAR EL EDULCORANTE PROTEINICO OBTENIDO. EL EDULCORANTE TIENE UNA SECUENCIA INTERCALADA ENTRE TIR (41) Y LEU (3) DE LA SECUENCIA SALVAJE DE MONELINA MODIFICADA PARA SUSTITUCION, DELECCION O INSERCION; Y ES ADECUADO PARA SU EMPLEO EN PRODUCTOS ENLATADOS, EN BEBIDAS CARBONATAS O PARA SU ADICION A CAFES, TES Y SIMILARES.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US6434187A | 1987-06-19 | 1987-06-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ES2012530A6 true ES2012530A6 (es) | 1990-04-01 |
Family
ID=22055274
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ES8801903A Expired - Lifetime ES2012530A6 (es) | 1987-06-19 | 1988-06-17 | Metodo de produccion de un edulcorante proteinico. |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0323489A1 (es) |
| JP (1) | JPH02500717A (es) |
| KR (1) | KR910008642B1 (es) |
| AU (1) | AU619193B2 (es) |
| BR (1) | BR8807102A (es) |
| ES (1) | ES2012530A6 (es) |
| GR (1) | GR1000110B (es) |
| IN (1) | IN168525B (es) |
| WO (1) | WO1988010303A1 (es) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5234834A (en) * | 1987-06-19 | 1993-08-10 | The Regents Of The University Of California | Constructs for expression of monellin in plant cells |
| US5739409A (en) * | 1987-06-19 | 1998-04-14 | The Regents Of The University Of California | Endogenously sweetened transgenic plant products |
| CA2006845C (en) * | 1988-12-29 | 1998-08-25 | Joong M. Cho | Expression of proteinaceous sweeteners in yeast |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3998798A (en) * | 1972-12-22 | 1976-12-21 | The Trustees Of The University Of Pennsylvania | Monellin, a sweet polypeptide derived from fruit of dioscoreophyllum cumminsii |
| ATE22327T1 (de) * | 1980-12-12 | 1986-10-15 | Unilever Nv | Dna-sequenzen fuer verschiedene allele formen des reifen thaumatins, diese sequenzen enthaltende rekombinante plasmide, verfahren zur herstellung dieser plasmide, diese rekombinante plasmide enthaltende bakterielle kulturen und verfahren zur herstellung von reifen thaumatin. |
| CA1310923C (en) * | 1985-11-13 | 1992-12-01 | Joachim Ludwig Weickmann | Dna encoding [asp --] and [lys -, asp --] thaumatin i |
| KR960012512B1 (ko) * | 1987-06-19 | 1996-09-20 | 캔디스 엘. 볼커 | 신규한 종류의 저칼로리 단백질 감미제 |
| JPH02500641A (ja) * | 1987-06-19 | 1990-03-08 | ラッキー バイオテク コーポレイション | 新規タンパク質性甘味剤の製造 |
-
1988
- 1988-06-02 AU AU19397/88A patent/AU619193B2/en not_active Ceased
- 1988-06-02 WO PCT/KR1988/000010 patent/WO1988010303A1/en not_active Application Discontinuation
- 1988-06-02 BR BR888807102A patent/BR8807102A/pt not_active Application Discontinuation
- 1988-06-02 JP JP63504731A patent/JPH02500717A/ja active Pending
- 1988-06-02 EP EP88905001A patent/EP0323489A1/en not_active Withdrawn
- 1988-06-02 KR KR1019890700263A patent/KR910008642B1/ko not_active Application Discontinuation
- 1988-06-06 IN IN460/CAL/88A patent/IN168525B/en unknown
- 1988-06-16 GR GR880100384A patent/GR1000110B/el not_active IP Right Cessation
- 1988-06-17 ES ES8801903A patent/ES2012530A6/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| KR890701740A (ko) | 1989-12-21 |
| GR1000110B (el) | 1991-06-07 |
| WO1988010303A1 (en) | 1988-12-29 |
| KR910008642B1 (ko) | 1991-10-19 |
| EP0323489A1 (en) | 1989-07-12 |
| BR8807102A (pt) | 1989-10-17 |
| JPH02500717A (ja) | 1990-03-15 |
| IN168525B (es) | 1991-04-20 |
| GR880100384A (en) | 1989-03-08 |
| AU619193B2 (en) | 1992-01-23 |
| AU1939788A (en) | 1989-01-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC1A | Transfer granted |
Owner name: LUCKY LTD. ( TITULAR DEL 50%) |
|
| PC1A | Transfer granted |