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ES2006611A6 - METHOD AND APPARATUS OF RESOLUTION FLUORESCENCE SPECTROSCOPY IN TIME. - Google Patents

METHOD AND APPARATUS OF RESOLUTION FLUORESCENCE SPECTROSCOPY IN TIME.

Info

Publication number
ES2006611A6
ES2006611A6 ES8800926A ES8800926A ES2006611A6 ES 2006611 A6 ES2006611 A6 ES 2006611A6 ES 8800926 A ES8800926 A ES 8800926A ES 8800926 A ES8800926 A ES 8800926A ES 2006611 A6 ES2006611 A6 ES 2006611A6
Authority
ES
Spain
Prior art keywords
fluorescence
time
fluorescence spectroscopy
current
optical image
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8800926A
Other languages
Spanish (es)
Inventor
Yong-Sheng Chao
Salvador M Fernandez
Ernest F Guignon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chimerix Corp
Original Assignee
Chimerix Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/031,288 external-priority patent/US4855930A/en
Priority claimed from US07/031,408 external-priority patent/US4923819A/en
Application filed by Chimerix Corp filed Critical Chimerix Corp
Publication of ES2006611A6 publication Critical patent/ES2006611A6/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5302—Apparatus specially adapted for immunological test procedures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6408—Fluorescence; Phosphorescence with measurement of decay time, time resolved fluorescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/06—Illumination; Optics
    • G01N2201/069—Supply of sources
    • G01N2201/0696—Pulsed
    • G01N2201/0697—Pulsed lasers

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Hematology (AREA)
  • Pathology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)

Abstract

METODO Y APARATO DE ESPECTROSCOPIA DE FLUORESCENCIA DE RESOLUCION EN EL TIEMPO, EN DONDE SE UTILIZA LUZ LASERICA DE UN SOLO IMPULSO PARA EXCITAR FOTONES FLUORESCENTES EN UNA MUESTRA, CUYA FLUORESCENCIA SE DETECTA MEDIANTE UN PMT OPTIMIZADO RESPECTO A LA LINEARIDAD Y TIEMPO DE RESPUESTA, PARA PRODUCIR FOTOELECTRONES QUE GENERAN UNA CORRIENTE EN EL ANODO DEL PMT. ESTA CORRIENTE SE DESCARGA A TRAVES DE UNA RED R/C PARA PRODUCIR UNA FORMA DE ONDA DE AMPLITUD DE VOLTAJE QUE SE CONVIERTEN A UNA IMAGEN OPTICA, SE INTENSIFICA, SE ALMACENA Y SE DIGITALIZA. LA VERSION DIGITALIZADA DE LA IMAGEN OPTICA SE PROCESA EN UN PROCESADOR DE DATOS PARA CALCULAR LA VERDADERA RESPUESTA AL IMPULSO DE LA FLUORESCENCIA. TAMBIEN SE DESCRIBE UN INMUNOANALISIS DE FLUORESCENCIA PARA DETERMINAR UN SOLO O VARIOS ANALITOS EN BASE A UNA UNICA MEDICION DE LA FLUORESCENCIA.METHOD AND APPARATUS OF RESOLUTION FLUORESCENCE SPECTROSCOPY IN TIME, WHERE LASER LIGHT OF A SINGLE IMPULSE IS USED TO EXCIT FLUORESCENT PHOTONS IN A SAMPLE, WHICH IS PROJECTED BY AN OPTIMAL LINE, AND SO ONLY PHOTOELECTRONS THAT GENERATE A CURRENT IN THE PMT ANODE. THIS CURRENT IS DOWNLOADED THROUGH AN R / C NETWORK TO PRODUCE A VOLTAGE AMPLITUDE WAVE SHAPE THAT CONVERTS TO AN OPTICAL IMAGE, IS INTENSIFIED, STORED AND DIGITIZED. THE DIGITIZED VERSION OF THE OPTICAL IMAGE IS PROCESSED IN A DATA PROCESSOR TO CALCULATE THE TRUE RESPONSE TO THE IMPULSE OF FLUORESCENCE. A FLUORESCENCE IMMUNOANALYSIS IS ALSO DESCRIBED TO DETERMINE A SOLE OR MULTIPLE ANALYZES BASED ON A SINGLE MEASUREMENT OF FLUORESCENCE.

ES8800926A 1987-03-27 1988-03-25 METHOD AND APPARATUS OF RESOLUTION FLUORESCENCE SPECTROSCOPY IN TIME. Expired ES2006611A6 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/031,288 US4855930A (en) 1987-03-27 1987-03-27 Method and appartatus for improved time-resolved fluorescence spectroscopy
US07/031,408 US4923819A (en) 1987-03-27 1987-03-27 Time-resolved fluorescence immunoassay

Publications (1)

Publication Number Publication Date
ES2006611A6 true ES2006611A6 (en) 1989-05-01

Family

ID=26707047

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8800926A Expired ES2006611A6 (en) 1987-03-27 1988-03-25 METHOD AND APPARATUS OF RESOLUTION FLUORESCENCE SPECTROSCOPY IN TIME.

Country Status (2)

Country Link
ES (1) ES2006611A6 (en)
WO (1) WO1988007670A2 (en)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2224352B (en) * 1988-10-05 1992-09-16 Hamamatsu Photonics Kk Light waveform measuring apparatus
FI82144C (en) * 1989-03-22 1991-01-10 Wallac Oy FOERFARANDE FOER SAMTIDIG BESTAEMNING AV FLERA LIGANDER.
US4977325A (en) * 1989-07-12 1990-12-11 P B Diagnostic Systems, Inc. Optical read system and immunoassay method
CA2026257C (en) * 1989-09-26 2001-07-24 Keiji Sasaki Device for analyzing a transient waveform
DE19718016A1 (en) * 1997-04-29 1998-11-05 Drexhage Karl Heinz Method for the optical detection of analyte molecules in a natural biological medium
GB0001089D0 (en) 2000-01-18 2000-03-08 Council Cent Lab Res Councils Lipoprotein assay
DE10308814A1 (en) * 2003-02-27 2004-09-09 Chromeon Gmbh Bioanalytical method based on the measurement of the decay time of phosphorescence
RU2264610C2 (en) * 2004-01-16 2005-11-20 Общество с ограниченной ответственностью "ВИНТЕЛ" Method for measuring spectroscopic properties of free-flowing substances and device for realization of said method
RU2253859C1 (en) * 2004-08-25 2005-06-10 Государственное образовательное учреждение высшего профессионального образования "Оренбургский государственный университет" Method of inspecting quality of liquid mixtures by means of registration of retarded fluorescence
AU2011280967B2 (en) * 2010-07-23 2014-09-11 Luminex Corporation Co-coupling to control reactivity of reagents in immunoassays
EP3381558A1 (en) 2012-03-16 2018-10-03 Stat-Diagnostica & Innovation, S.L. A method for a test cartridge with integrated transfer module
CN103558433B (en) * 2013-10-24 2016-03-30 北京无线电计量测试研究所 A kind of device and method measured for photodetector impulse response
CN103728446B (en) * 2013-12-11 2016-02-24 天津大学 Photon counting-type hyperchannel time resolved fluoro-immunoassay system and method for counting

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4385126A (en) * 1980-11-19 1983-05-24 International Diagnostic Technology, Inc. Double tagged immunoassay
US4368047A (en) * 1981-04-27 1983-01-11 University Of Utah Research Foundation Process for conducting fluorescence immunoassays without added labels and employing attenuated internal reflection
US4630925A (en) * 1982-11-01 1986-12-23 Hamamatsu Corp. Compact temporal spectral photometer
US5238808A (en) * 1984-10-31 1993-08-24 Igen, Inc. Luminescent metal chelate labels and means for detection
JPH06128844A (en) * 1992-10-20 1994-05-10 Tsudakoma Corp Separator for defective weft yarn in multicolor rapier loom

Also Published As

Publication number Publication date
WO1988007670A2 (en) 1988-10-06
WO1988007670A3 (en) 1989-01-26

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19991102