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ES2005168A6 - Procedimiento para la produccion de polideoxirribonucleotido defibrotide - Google Patents

Procedimiento para la produccion de polideoxirribonucleotido defibrotide

Info

Publication number
ES2005168A6
ES2005168A6 ES8701114A ES8701114A ES2005168A6 ES 2005168 A6 ES2005168 A6 ES 2005168A6 ES 8701114 A ES8701114 A ES 8701114A ES 8701114 A ES8701114 A ES 8701114A ES 2005168 A6 ES2005168 A6 ES 2005168A6
Authority
ES
Spain
Prior art keywords
monomer
polydeoxyribonucleotides
reproducible
defibrotide
depolymerization
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8701114A
Other languages
English (en)
Inventor
Gianfranco Fedeli
Giuseppe Diamantini
Marisa Mantovani
Giuseppe Prino
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Crinos Industria Farmacobiologica SpA
Original Assignee
Crinos Industria Farmacobiologica SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Crinos Industria Farmacobiologica SpA filed Critical Crinos Industria Farmacobiologica SpA
Publication of ES2005168A6 publication Critical patent/ES2005168A6/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H1/00—Processes for the preparation of sugar derivatives
    • C07H1/06—Separation; Purification
    • C07H1/08—Separation; Purification from natural products
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/117—Nucleic acids having immunomodulatory properties, e.g. containing CpG-motifs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/17—Immunomodulatory nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

PROCEDIMIENTO PARA LA PRODUCCION DE POLIDEOXIRRIBONUCLEOTIDO DEFIBROTIDE. SE OBTIENE DESPOLIMERIZACION DE SOLUCIONES ESTABILIZADAS DE POLIDEOXIRRIBONUCLEOTIDOS ALTAMENTE POLIMERIZADOS E INTERRUMPIDOS, TAL Y COMO SE OBTIENEN POR MEDIO DE ACIDOS NUCLEOTIDOS DE PARTIDA, EFECTUANDOSE LA DESPOLIMERIZACION POR CALENTAMIENTO A TEMPERATURA CONTROLADA Y SIENDO CONTROLADO COMO UNA FUNCION DE LA VARIACION DE LA HIPERCROMICIDAD REVERSIBLE, SEGUIDO POR SEPARACION DE LOS ENLACES HIDROGENO, EN LOS FILAMENTOS DE DOBLE HEBRA Y POR ESTABILIZACION TERMICA DE LOS FILAMENTOS DE UNA SOLA HEBRA.EL DEFIBROTIDE PRESENTA LA SIGUIENTE FORMULA DE SECUENCIA ALEATORIA:P1-5, (DAP)12-24, (DGP)10-20, (DTP)13-26, (DCP)10-20 EN DONDE P ES EL RADICAL FOSFORICO, DAP ES EL MONOMERO DEOXIADENILICO, DGP ES EL MONOMERO DEOXIGUANILICO, DTP ES EL MONOMERO DEOXITIMIDILICO, Y DCP ES EL MONOMERO DEOXICITIDILICO, Y EXHIBE PROPIEDADES QUIMICO-FISICAS BIEN DEFINIDAS, REPRODUCIBLES A ESCALA DE PRODUCCION INDUSTRIAL.
ES8701114A 1986-04-17 1987-04-15 Procedimiento para la produccion de polideoxirribonucleotido defibrotide Expired ES2005168A6 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT20117/86A IT1190313B (it) 1986-04-17 1986-04-17 Procedimento per l'ottenimento di polidesossiribonucleotidi chimicamente definiti e riproducibili e prodotto farmacologicamente attivo risultante

Publications (1)

Publication Number Publication Date
ES2005168A6 true ES2005168A6 (es) 1989-03-01

Family

ID=11163944

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8701114A Expired ES2005168A6 (es) 1986-04-17 1987-04-15 Procedimiento para la produccion de polideoxirribonucleotido defibrotide

Country Status (20)

Country Link
US (1) US4985552A (es)
EP (1) EP0263155B1 (es)
JP (1) JP2581722B2 (es)
KR (1) KR880701242A (es)
CN (1) CN1018650B (es)
AR (1) AR242585A1 (es)
AT (1) ATE66927T1 (es)
AU (1) AU612976B2 (es)
BE (1) BE1002908A4 (es)
CA (1) CA1297430C (es)
CH (1) CH677114A5 (es)
DE (1) DE3772697D1 (es)
ES (1) ES2005168A6 (es)
FR (1) FR2597481B1 (es)
GR (1) GR870602B (es)
IL (1) IL82251A (es)
IT (1) IT1190313B (es)
MX (1) MX168887B (es)
WO (1) WO1987006235A1 (es)
ZA (1) ZA872755B (es)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5182269A (en) * 1987-09-23 1993-01-26 Crinos Industria Farmacobiologica Spa Composition for topical use having hair stimulating, anti-dandruff and anti-seborrhoic activity
IT1252174B (it) * 1991-12-09 1995-06-05 Crinos Industria Farmaco Oligodesossimibonucleotidi ad attivita' antiischemica e procedimenti per il loro ottenimento
IT1271685B (it) * 1994-08-03 1997-06-04 Crinos Industria Farmaco Impiego dei polidesossiribonucleotidi nelle neuropati diabetiche
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
DK1059092T3 (da) 1999-06-08 2006-03-27 Gentium Spa Anvendelse af komplekser af kationiske liposomer og polydeoxyribonukleotider som medikamenter
EP1147777A1 (en) 2000-04-18 2001-10-24 Crinos Industria Farmacobiologica S.p.A. Combination of defibrotide and G-CSF and its use to activate haematopoietic progenitors
US20020127587A1 (en) * 2001-02-13 2002-09-12 Domenica Simms Methods and compositions for isolation of biological macromolecules
CA2498518A1 (en) 2002-09-13 2004-03-25 Valentis, Inc. Apparatus and method for preparative scale purification of nucleic acids
EP2216415B2 (en) * 2003-08-01 2017-01-04 Life Technologies Corporation Methods for preparing short RNA molecules
ITMI20031714A1 (it) * 2003-09-05 2005-03-06 Gentium Spa Formazioni ad azione antitumorale.
MX2007010407A (es) * 2005-03-03 2007-10-17 Gentium Spa Formulacion con accion anti-tumor.
EP2103689A1 (en) * 2008-03-19 2009-09-23 Gentium S.p.A. Synthetic phosphodiester oligonucleotides and therapeutical uses thereof
ES2694239T3 (es) 2010-11-12 2018-12-19 Gentium S.R.L. Defibrótido para su uso en profilaxis y/o tratamiento de la enfermedad de Injerto contra huésped (GVHD)
JP6198821B2 (ja) 2012-06-22 2017-09-20 ゲンチウム エス．アール．エル． デフィブロチドの生物学的活性を決定する為の、ユーグロブリンに基づく方法
EP3026122A1 (en) 2014-11-27 2016-06-01 Gentium S.p.A. Cellular-based method for determining the potency of defibrotide
AR112403A1 (es) 2017-08-03 2019-10-23 Jazz Pharmaceuticals Ireland Ltd Formulaciones de ácidos nucleicos altamente concentradas
US11571440B2 (en) 2018-04-12 2023-02-07 Jazz Pharmaceuticals Ireland Limited Defibrotide for the prevention and treatment of cytokine release syndrome and neurotoxicity associated with immunodepletion
WO2019213053A1 (en) 2018-04-30 2019-11-07 Gentium S.R.L. Methods for treating patients having sinusoidal obstruction syndrome
US20220023533A1 (en) 2018-12-07 2022-01-27 Jazz Phrmaceticals Ireland Limited Subcutaneous delivery of high concentration formulations
US20230190783A1 (en) 2020-02-28 2023-06-22 Jazz Pharmaceuticals Ireland Limited Delivery of low viscosity formulations
WO2021212055A1 (en) 2020-04-17 2021-10-21 Jazz Pharmaceuticals Ireland Limited Defibrotide treatment for the prevention of organ rejection and injury
TW202308659A (zh) 2021-05-06 2023-03-01 愛爾蘭商爵士製藥愛爾蘭有限責任公司 用於急性呼吸窘迫症候群之治療及預防的去纖維蛋白多核苷酸

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1371944A (fr) * 1963-07-30 1964-09-11 Centre Nat Rech Scient Procédé de préparation d'acides désoxyribonucléiques hautement polymérisés
IT1043823B (it) * 1970-11-03 1980-02-29 Prephar Procedimento per l estrazione di acidi nucleici da organi animali
US3899481A (en) * 1970-11-03 1975-08-12 Crinos Industria Farmaco Process for the controlled partial degradation of deoxyribonucleic acid extracted from animal organs
DE2154279A1 (de) * 1970-11-03 1972-05-25 Crinos Industria Farmaco Medikamente für das fibrinolytische System
CH617963A5 (es) * 1973-10-05 1980-06-30 Derivati Organici Lab
SU652187A1 (ru) * 1975-07-23 1979-03-15 Киевский Ордена Ленина Государственный Университет Им. Т.Г.Шевченко Способ получени дезоксирибонуклеиновой кислоты
JPS554308A (en) * 1978-06-23 1980-01-12 Yuki Gosei Yakuhin Kogyo Kk Isolation of deoxyribonucleic acid from milt of fish
IT1170215B (it) * 1983-09-12 1987-06-03 Crinos Industria Farmaco Composizione farmaceutica per il trattamento di stati di insufficienza renale acuta
IT1206341B (it) * 1984-02-16 1989-04-14 Crinos Industria Farmaco Composizione farmaceutica per il trattamento dell'ischemia acuta del miocardio.

Also Published As

Publication number Publication date
FR2597481B1 (fr) 1990-01-12
CN1018650B (zh) 1992-10-14
ATE66927T1 (de) 1991-09-15
IT8620117A1 (it) 1987-10-17
IT8620117A0 (it) 1986-04-17
MX168887B (es) 1993-06-14
DE3772697D1 (de) 1991-10-10
CA1297430C (en) 1992-03-17
JPS63503067A (ja) 1988-11-10
IL82251A0 (en) 1987-10-30
EP0263155B1 (en) 1991-09-04
IT1190313B (it) 1988-02-16
EP0263155A1 (en) 1988-04-13
IL82251A (en) 1991-05-12
KR880701242A (ko) 1988-07-26
WO1987006235A1 (en) 1987-10-22
BE1002908A4 (fr) 1991-07-30
JP2581722B2 (ja) 1997-02-12
GR870602B (en) 1987-08-17
ZA872755B (en) 1987-10-05
AU612976B2 (en) 1991-07-25
AU7288687A (en) 1987-11-09
FR2597481A1 (fr) 1987-10-23
CN87103688A (zh) 1988-01-20
AR242585A1 (es) 1993-04-30
CH677114A5 (es) 1991-04-15
US4985552A (en) 1991-01-15

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Legal Events

Date Code Title Description
PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20070416