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ES2004066A6 - A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells - Google Patents

A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells

Info

Publication number
ES2004066A6
ES2004066A6 ES8700144A ES8700144A ES2004066A6 ES 2004066 A6 ES2004066 A6 ES 2004066A6 ES 8700144 A ES8700144 A ES 8700144A ES 8700144 A ES8700144 A ES 8700144A ES 2004066 A6 ES2004066 A6 ES 2004066A6
Authority
ES
Spain
Prior art keywords
antigen
reagents
enzyme
kit
detected
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES8700144A
Other languages
Spanish (es)
Inventor
Karen Kvist Christensen
Poul Christensen
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EURO FASSEL AB
Original Assignee
EURO FASSEL AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EURO FASSEL AB filed Critical EURO FASSEL AB
Priority to ES8700144A priority Critical patent/ES2004066A6/en
Priority to AU11899/88A priority patent/AU1189988A/en
Priority to PCT/SE1988/000011 priority patent/WO1988005540A1/en
Publication of ES2004066A6 publication Critical patent/ES2004066A6/en
Expired legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals

Landscapes

  • Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Hematology (AREA)
  • Urology & Nephrology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

The reagents in the kit are included in an absorbant material, with the reagents in dry alternatively lyophilized form. The reagents are arranged in one of two ways. In Arrangement I the order of the reagents is (1) purified complexes of enzyme and antibodies against the antigen to be detected (2) antigen immobilized in the absorbant material and (3) substrate to the enzyme. In Arrangement II the order of reagents is (1) purified complexes of enzyme and antigen, which antigen is antigenically related to the antigen to be detected and which antigen is immobilized in the absorbant material and (3) substrate to the enzyme. Arrangement I can also be used to detect antibody. In both cases, the test fluid is allowed to pass, by capillary forces, from step (1) to step (3). The presence of the antigen (antibody) to be tested for is thereby detected by the occurrence of a colour reaction at (3), due to the splitting of the substrate. The kit therefore allows a very simple procedure for detection of a compound without washing steps etc. usually included in the ELISA-technique. The invention also creates possibilities for quantitation by instrumental reading of the colour change at step (3).
ES8700144A 1987-01-21 1987-01-21 A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells Expired ES2004066A6 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
ES8700144A ES2004066A6 (en) 1987-01-21 1987-01-21 A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells
AU11899/88A AU1189988A (en) 1987-01-21 1988-01-19 A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells
PCT/SE1988/000011 WO1988005540A1 (en) 1987-01-21 1988-01-19 A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ES8700144A ES2004066A6 (en) 1987-01-21 1987-01-21 A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells

Publications (1)

Publication Number Publication Date
ES2004066A6 true ES2004066A6 (en) 1988-12-01

Family

ID=8249199

Family Applications (1)

Application Number Title Priority Date Filing Date
ES8700144A Expired ES2004066A6 (en) 1987-01-21 1987-01-21 A kit for qualitative and quantitative analysis of antigens, antibodies and/or microorganisms or other cells

Country Status (3)

Country Link
AU (1) AU1189988A (en)
ES (1) ES2004066A6 (en)
WO (1) WO1988005540A1 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ507994A (en) * 1998-05-15 2003-01-31 Sekisui Chemical Co Ltd Immunoassay reagent and immunoassay method
EP1243927A1 (en) * 2001-03-23 2002-09-25 Future Diagnostics B.V. Short turnaround time insulin assay

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4446232A (en) * 1981-10-13 1984-05-01 Liotta Lance A Enzyme immunoassay with two-zoned device having bound antigens
CA1261743A (en) * 1984-07-23 1989-09-26 Shai Inbar Biological diagnostic assay product, and process utilizing labeled fab fragments
DE3445816C1 (en) * 1984-12-15 1986-06-12 Behringwerke Ag, 3550 Marburg Flat diagnostic agent
IT1200382B (en) * 1985-02-08 1989-01-18 Boehringer Biochemia Srl DETECTION AND / OR DOSAGE SYSTEM OF CLINICAL PARAMETERS BY IMMUNO ENZYMATIC ROUTE
US4670381A (en) * 1985-07-19 1987-06-02 Eastman Kodak Company Heterogeneous immunoassay utilizing horizontal separation in an analytical element
GB8526741D0 (en) * 1985-10-30 1985-12-04 Boots Celltech Diagnostics Binding assay device
JPH0814582B2 (en) * 1986-02-20 1996-02-14 富士写真フイルム株式会社 Multilayer analytical element for immunoreactant quantification
CA1289872C (en) * 1986-06-18 1991-10-01 David L. Woodrum Reversible immobilization of assay reagents in a multizone test device

Also Published As

Publication number Publication date
WO1988005540A1 (en) 1988-07-28
AU1189988A (en) 1988-08-10

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Legal Events

Date Code Title Description
FD1A Patent lapsed

Effective date: 19991108