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EP4234566A4 - Modifiziertes nukleosid oder nukleotid - Google Patents

Modifiziertes nukleosid oder nukleotid Download PDF

Info

Publication number
EP4234566A4
EP4234566A4 EP21882101.5A EP21882101A EP4234566A4 EP 4234566 A4 EP4234566 A4 EP 4234566A4 EP 21882101 A EP21882101 A EP 21882101A EP 4234566 A4 EP4234566 A4 EP 4234566A4
Authority
EP
European Patent Office
Prior art keywords
nucleotide
modified nucleoside
nucleoside
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21882101.5A
Other languages
English (en)
French (fr)
Other versions
EP4234566A1 (de
Inventor
Xun Xu
Bo TENG
Wenwei Zhang
Ao CHEN
Handong Li
Shengyi YAN
Shitian ZHUO
Liang Shen
Yonghui Zhang
Nanfeng GAO
Jie Zhao
Sha LIAO
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bgi Shenzhen Group Holdings Co Ltd
BGI Shenzhen Co Ltd
Original Assignee
Bgi Shenzhen Group Holdings Co Ltd
BGI Shenzhen Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bgi Shenzhen Group Holdings Co Ltd, BGI Shenzhen Co Ltd filed Critical Bgi Shenzhen Group Holdings Co Ltd
Publication of EP4234566A1 publication Critical patent/EP4234566A1/de
Publication of EP4234566A4 publication Critical patent/EP4234566A4/de
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • C07H19/10Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2535/00Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
    • C12Q2535/122Massive parallel sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2563/00Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/55Design of synthesis routes, e.g. reducing the use of auxiliary or protecting groups

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP21882101.5A 2020-10-21 2021-10-21 Modifiziertes nukleosid oder nukleotid Pending EP4234566A4 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN202011134947 2020-10-21
PCT/CN2021/125262 WO2022083686A1 (zh) 2020-10-21 2021-10-21 修饰的核苷或核苷酸

Publications (2)

Publication Number Publication Date
EP4234566A1 EP4234566A1 (de) 2023-08-30
EP4234566A4 true EP4234566A4 (de) 2024-12-11

Family

ID=81289694

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21882101.5A Pending EP4234566A4 (de) 2020-10-21 2021-10-21 Modifiziertes nukleosid oder nukleotid

Country Status (8)

Country Link
US (1) US20240067673A1 (de)
EP (1) EP4234566A4 (de)
JP (1) JP2023546741A (de)
KR (1) KR20230107259A (de)
CN (1) CN116507736A (de)
AU (1) AU2021363336A1 (de)
CA (1) CA3199278A1 (de)
WO (1) WO2022083686A1 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115197290B (zh) * 2021-04-01 2025-03-07 深圳华大生命科学研究院 用于测序的核苷酸类似物
WO2024123866A1 (en) * 2022-12-09 2024-06-13 Illumina, Inc. Nucleosides and nucleotides with 3´ blocking groups and cleavable linkers

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023064A1 (fr) * 1993-03-26 1994-10-13 Institut Pasteur Nouveaux derives utilisables en sequençage d'acides nucleiques
WO1996007669A1 (en) * 1994-09-02 1996-03-14 Rose-Hiatt Biotechnology Partnership Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
WO2002029003A2 (en) * 2000-10-06 2002-04-11 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
WO2020159447A1 (en) * 2019-01-31 2020-08-06 Agency For Science, Technology And Research Method of synthesizing single-stranded nucleotide sequence, blocked nucleoside triphosphates and related methods
WO2021067970A1 (en) * 2019-10-04 2021-04-08 Centrillion Technologies, Inc. Reversible terminators for dna sequencing and methods of using the same

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2008037568A2 (en) * 2006-09-04 2008-04-03 Quiatech Ab Reversible terminators for efficient sequencing by synthesis
US8603741B2 (en) * 2010-02-18 2013-12-10 Pacific Biosciences Of California, Inc. Single molecule sequencing with two distinct chemistry steps
KR102230831B1 (ko) * 2013-03-15 2021-03-22 일루미나 케임브리지 리미티드 변형된 뉴클레오사이드 또는 뉴클레오타이드
CN109983136B (zh) * 2016-12-13 2022-07-22 深圳华大智造科技股份有限公司 提纯可逆阻断脱氧核糖核苷三磷酸的方法和测序方法
IL267836B2 (en) 2017-01-04 2023-09-01 Complete Genomics Inc Stepwise sequence determination by unlabeled reversible terminations or natural nucleotides
WO2019071474A1 (zh) * 2017-10-11 2019-04-18 深圳华大智造科技有限公司 修饰的核苷或核苷酸

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994023064A1 (fr) * 1993-03-26 1994-10-13 Institut Pasteur Nouveaux derives utilisables en sequençage d'acides nucleiques
WO1996007669A1 (en) * 1994-09-02 1996-03-14 Rose-Hiatt Biotechnology Partnership Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides
WO2002029003A2 (en) * 2000-10-06 2002-04-11 The Trustees Of Columbia University In The City Of New York Massive parallel method for decoding dna and rna
WO2020159447A1 (en) * 2019-01-31 2020-08-06 Agency For Science, Technology And Research Method of synthesizing single-stranded nucleotide sequence, blocked nucleoside triphosphates and related methods
WO2021067970A1 (en) * 2019-10-04 2021-04-08 Centrillion Technologies, Inc. Reversible terminators for dna sequencing and methods of using the same

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
CANARD BRUNO ET AL: "DNA polymerase fluorescent substrates with reversible 3'-tags", GENE 148 (1994), 2 June 1994 (1994-06-02), pages 1 - 6, XP093216361, DOI: https://doi.org/10.1016/0378-1119(94)90226-7 *
See also references of WO2022083686A1 *

Also Published As

Publication number Publication date
US20240067673A1 (en) 2024-02-29
KR20230107259A (ko) 2023-07-14
CN116507736A (zh) 2023-07-28
JP2023546741A (ja) 2023-11-07
CA3199278A1 (en) 2022-04-28
AU2021363336A9 (en) 2024-09-05
WO2022083686A1 (zh) 2022-04-28
AU2021363336A1 (en) 2023-05-18
EP4234566A1 (de) 2023-08-30

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