EP4211223A4 - METHODS FOR LYSING BACTERIAL CELLS - Google Patents
METHODS FOR LYSING BACTERIAL CELLS Download PDFInfo
- Publication number
- EP4211223A4 EP4211223A4 EP21867584.1A EP21867584A EP4211223A4 EP 4211223 A4 EP4211223 A4 EP 4211223A4 EP 21867584 A EP21867584 A EP 21867584A EP 4211223 A4 EP4211223 A4 EP 4211223A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- methods
- bacterial cells
- lysing bacterial
- lysing
- cells
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 230000001580 bacterial effect Effects 0.000 title 1
- 230000002934 lysing effect Effects 0.000 title 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/06—Lysis of microorganisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/64—General methods for preparing the vector, for introducing it into the cell or for selecting the vector-containing host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/70—Vectors or expression systems specially adapted for E. coli
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/185—Escherichia
- C12R2001/19—Escherichia coli
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Cell Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202010949684.5A CN114164113A (en) | 2020-09-10 | 2020-09-10 | A closed cell lysis process |
PCT/US2021/049633 WO2022056106A1 (en) | 2020-09-10 | 2021-09-09 | Methods for lysing bacterial cells |
Publications (2)
Publication Number | Publication Date |
---|---|
EP4211223A1 EP4211223A1 (en) | 2023-07-19 |
EP4211223A4 true EP4211223A4 (en) | 2024-10-16 |
Family
ID=80475865
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
EP21867584.1A Pending EP4211223A4 (en) | 2020-09-10 | 2021-09-09 | METHODS FOR LYSING BACTERIAL CELLS |
Country Status (5)
Country | Link |
---|---|
US (1) | US20230340398A1 (en) |
EP (1) | EP4211223A4 (en) |
JP (1) | JP2023541864A (en) |
CN (1) | CN114164113A (en) |
WO (1) | WO2022056106A1 (en) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998043724A1 (en) * | 1997-03-31 | 1998-10-08 | Takara Shuzo Co., Ltd. | Isolation apparatus |
US20090246859A1 (en) * | 2003-03-12 | 2009-10-01 | Qiagen North American Holdings, Inc. | Methods and Compositions for Purification of Nucleic Acid from a Host Cell |
RU2408729C2 (en) * | 2009-04-06 | 2011-01-10 | Учреждение Российской академии наук Институт химической биологии и фундаментальной медицины Сибирского отделения РАН (ИХБФМ СО РАН) | Method for production of plasmid dna from bacterial cells |
CN103525866A (en) * | 2013-03-22 | 2014-01-22 | 人福医药集团股份公司 | Method for preparing plasmids |
EP2364769B1 (en) * | 2003-05-30 | 2016-01-13 | VGXI, Inc. | Devices and methods for biomaterial production |
Family Cites Families (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4833239A (en) * | 1987-01-29 | 1989-05-23 | Genetics Institute, Inc. | Method for the isolation and purification of DNA molecules |
CA2527216A1 (en) * | 2003-05-28 | 2004-12-09 | Qiagen Gmbh | Process for obtaining plasmid dna by means of an aqueous 2-phase system |
US20050079534A1 (en) * | 2003-10-08 | 2005-04-14 | Akzo Nobel, N.V. | Lysis and neutralization method for production of pharmaceutical grade plasmid DNA |
EP2160459A2 (en) * | 2007-05-23 | 2010-03-10 | Nature Technology Corp. | Improved e. coli plasmid dna production |
CN109370963A (en) * | 2018-09-21 | 2019-02-22 | 安徽欣伯玉生物科技有限公司 | A kind of cultural method of colibacillus engineering high efficient expression supercoiled plasmid DNA |
-
2020
- 2020-09-10 CN CN202010949684.5A patent/CN114164113A/en active Pending
-
2021
- 2021-09-09 WO PCT/US2021/049633 patent/WO2022056106A1/en unknown
- 2021-09-09 JP JP2023515685A patent/JP2023541864A/en active Pending
- 2021-09-09 US US18/044,263 patent/US20230340398A1/en active Pending
- 2021-09-09 EP EP21867584.1A patent/EP4211223A4/en active Pending
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO1998043724A1 (en) * | 1997-03-31 | 1998-10-08 | Takara Shuzo Co., Ltd. | Isolation apparatus |
US20090246859A1 (en) * | 2003-03-12 | 2009-10-01 | Qiagen North American Holdings, Inc. | Methods and Compositions for Purification of Nucleic Acid from a Host Cell |
EP2364769B1 (en) * | 2003-05-30 | 2016-01-13 | VGXI, Inc. | Devices and methods for biomaterial production |
RU2408729C2 (en) * | 2009-04-06 | 2011-01-10 | Учреждение Российской академии наук Институт химической биологии и фундаментальной медицины Сибирского отделения РАН (ИХБФМ СО РАН) | Method for production of plasmid dna from bacterial cells |
CN103525866A (en) * | 2013-03-22 | 2014-01-22 | 人福医药集团股份公司 | Method for preparing plasmids |
Non-Patent Citations (2)
Title |
---|
MEACLE F.J. ET AL: "Impact of engineering flow conditions on plasmid DNA yield and purity in chemical cell lysis operations", BIOTECHNOLOGY AND BIOENGINEERING, vol. 87, no. 3, 5 August 2004 (2004-08-05), Hoboken, USA, pages 293 - 302, XP093198744, ISSN: 0006-3592, DOI: 10.1002/bit.20114 * |
See also references of WO2022056106A1 * |
Also Published As
Publication number | Publication date |
---|---|
WO2022056106A1 (en) | 2022-03-17 |
EP4211223A1 (en) | 2023-07-19 |
CN114164113A (en) | 2022-03-11 |
JP2023541864A (en) | 2023-10-04 |
US20230340398A1 (en) | 2023-10-26 |
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PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
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Owner name: ABELZETA INC. |
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A4 | Supplementary search report drawn up and despatched |
Effective date: 20240912 |
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RIC1 | Information provided on ipc code assigned before grant |
Ipc: C12R 1/19 20060101ALI20240906BHEP Ipc: C12Q 1/6806 20180101ALI20240906BHEP Ipc: C12N 15/10 20060101ALI20240906BHEP Ipc: C12N 1/06 20060101AFI20240906BHEP |