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EP4172343A4 - GENOME EDITING OF TRANSGENIC CROPS WITH MODIFIED TRANSGENIC LOCI - Google Patents

GENOME EDITING OF TRANSGENIC CROPS WITH MODIFIED TRANSGENIC LOCI Download PDF

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Publication number
EP4172343A4
EP4172343A4 EP21851338.0A EP21851338A EP4172343A4 EP 4172343 A4 EP4172343 A4 EP 4172343A4 EP 21851338 A EP21851338 A EP 21851338A EP 4172343 A4 EP4172343 A4 EP 4172343A4
Authority
EP
European Patent Office
Prior art keywords
transgenic
genome editing
loci
modified
crops
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP21851338.0A
Other languages
German (de)
French (fr)
Other versions
EP4172343A1 (en
Inventor
Michael Andreas KOCK
Michael Lee NUCCIO
Frédéric VAN EX
Alexandra ELATA
Daniel RODRIGUEZ LEAL
Joshua L. Price
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Inari Agriculture Technology Inc
Original Assignee
Inari Agriculture Technology Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US17/248,936 external-priority patent/US11359210B2/en
Priority claimed from US17/249,640 external-priority patent/US11214811B1/en
Priority claimed from US17/302,110 external-priority patent/US20220030822A1/en
Priority claimed from US17/302,121 external-priority patent/US11242534B1/en
Priority claimed from US17/302,739 external-priority patent/US11326177B2/en
Priority claimed from US17/303,116 external-priority patent/US11369073B2/en
Application filed by Inari Agriculture Technology Inc filed Critical Inari Agriculture Technology Inc
Priority claimed from PCT/US2021/043161 external-priority patent/WO2022026375A1/en
Publication of EP4172343A1 publication Critical patent/EP4172343A1/en
Publication of EP4172343A4 publication Critical patent/EP4172343A4/en
Pending legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Medicinal Chemistry (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP21851338.0A 2020-07-31 2021-07-26 GENOME EDITING OF TRANSGENIC CROPS WITH MODIFIED TRANSGENIC LOCI Pending EP4172343A4 (en)

Applications Claiming Priority (18)

Application Number Priority Date Filing Date Title
US202063059916P 2020-07-31 2020-07-31
US202063059963P 2020-07-31 2020-07-31
US202063059813P 2020-07-31 2020-07-31
US202063059860P 2020-07-31 2020-07-31
US202163199930P 2021-02-03 2021-02-03
US202163199951P 2021-02-04 2021-02-04
US202163199949P 2021-02-04 2021-02-04
US17/248,936 US11359210B2 (en) 2020-07-31 2021-02-12 INIR12 transgenic maize
US17/249,640 US11214811B1 (en) 2020-07-31 2021-03-08 INIR6 transgenic maize
US202163201030P 2021-04-09 2021-04-09
US202163201029P 2021-04-09 2021-04-09
US17/302,110 US20220030822A1 (en) 2020-07-31 2021-04-23 Inht26 transgenic soybean
US17/302,121 US11242534B1 (en) 2020-07-31 2021-04-23 INHT31 transgenic soybean
US17/302,739 US11326177B2 (en) 2020-07-31 2021-05-11 INIR12 transgenic maize
US17/303,116 US11369073B2 (en) 2020-07-31 2021-05-20 INIR12 transgenic maize
US202163202569P 2021-06-16 2021-06-16
US202163203137P 2021-07-09 2021-07-09
PCT/US2021/043161 WO2022026375A1 (en) 2020-07-31 2021-07-26 Genome editing of transgenic crop plants with modified transgenic loci

Publications (2)

Publication Number Publication Date
EP4172343A1 EP4172343A1 (en) 2023-05-03
EP4172343A4 true EP4172343A4 (en) 2024-10-02

Family

ID=85128601

Family Applications (1)

Application Number Title Priority Date Filing Date
EP21851338.0A Pending EP4172343A4 (en) 2020-07-31 2021-07-26 GENOME EDITING OF TRANSGENIC CROPS WITH MODIFIED TRANSGENIC LOCI

Country Status (5)

Country Link
US (1) US20240294937A1 (en)
EP (1) EP4172343A4 (en)
CN (1) CN116367714A (en)
BR (1) BR112023001759A2 (en)
CA (1) CA3188283A1 (en)

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066780A2 (en) * 2000-03-09 2001-09-13 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
WO2002012517A1 (en) * 2000-08-04 2002-02-14 University Of Victoria Innovation And Development Corporation Plant promoter derived from luminal binding protein gene and methods for its use
WO2006003186A1 (en) * 2004-07-06 2006-01-12 Biogemma Method for enhancing gene expression in plants
WO2013116700A1 (en) * 2012-02-01 2013-08-08 Dow Agrosciences Llc Glyphosate resistant plants and associated methods
WO2015118640A1 (en) * 2014-02-06 2015-08-13 日本たばこ産業株式会社 Acquisition method for plant transformed cell
CN104846010A (en) * 2015-05-18 2015-08-19 安徽省农业科学院水稻研究所 Method for deleting selection marker gene of transgenic rice
WO2019238772A1 (en) * 2018-06-13 2019-12-19 Stichting Wageningen Research Polynucleotide constructs and methods of gene editing using cpf1

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066780A2 (en) * 2000-03-09 2001-09-13 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
WO2002012517A1 (en) * 2000-08-04 2002-02-14 University Of Victoria Innovation And Development Corporation Plant promoter derived from luminal binding protein gene and methods for its use
WO2006003186A1 (en) * 2004-07-06 2006-01-12 Biogemma Method for enhancing gene expression in plants
WO2013116700A1 (en) * 2012-02-01 2013-08-08 Dow Agrosciences Llc Glyphosate resistant plants and associated methods
WO2015118640A1 (en) * 2014-02-06 2015-08-13 日本たばこ産業株式会社 Acquisition method for plant transformed cell
CN104846010A (en) * 2015-05-18 2015-08-19 安徽省农业科学院水稻研究所 Method for deleting selection marker gene of transgenic rice
WO2019238772A1 (en) * 2018-06-13 2019-12-19 Stichting Wageningen Research Polynucleotide constructs and methods of gene editing using cpf1

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
CHRISTIAN MICHELLE ET AL: "Targeted mutagenesis of Arabidopsis thaliana using engineered TAL effector nucleases", G3 (BETHESDA, MD.), 3 October 2013 (2013-10-03), United States, pages 1697 - 1705, XP055902232, Retrieved from the Internet <URL:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3789794/pdf/1697.pdf> [retrieved on 20220316], DOI: 10.1534/g3.113.007104 *
OW ET AL: "GM maize from site-specific recombination technology, what next?", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 18, no. 2, 17 April 2007 (2007-04-17), pages 115 - 120, XP022032089, ISSN: 0958-1669, DOI: 10.1016/J.COPBIO.2007.02.004 *
See also references of WO2022026375A1 *
SRIVASTAVA VIBHA ET AL: "Dual-targeting by CRISPR/Cas9 for precise excision of transgenes from rice genome", PLANT CELL, TISSUE AND ORGAN CULTURE (PCTOC), SPRINGER NETHERLANDS, DORDRECHT, vol. 129, no. 1, 20 January 2017 (2017-01-20), pages 153 - 160, XP036194323, ISSN: 0167-6857, [retrieved on 20170120], DOI: 10.1007/S11240-016-1166-3 *

Also Published As

Publication number Publication date
CA3188283A1 (en) 2022-02-03
CN116367714A (en) 2023-06-30
US20240294937A1 (en) 2024-09-05
BR112023001759A2 (en) 2023-03-07
EP4172343A1 (en) 2023-05-03

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