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EP3450451A4 - MODIFIED FIBROIN - Google Patents

MODIFIED FIBROIN Download PDF

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Publication number
EP3450451A4
EP3450451A4 EP17789711.3A EP17789711A EP3450451A4 EP 3450451 A4 EP3450451 A4 EP 3450451A4 EP 17789711 A EP17789711 A EP 17789711A EP 3450451 A4 EP3450451 A4 EP 3450451A4
Authority
EP
European Patent Office
Prior art keywords
modified fibroin
fibroin
modified
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
EP17789711.3A
Other languages
German (de)
French (fr)
Other versions
EP3450451A1 (en
Inventor
Keisuke Morita
Junichi Sugahara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kojima Industries Corp
Spiber Inc
Original Assignee
Kojima Industries Corp
Spiber Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kojima Industries Corp, Spiber Inc filed Critical Kojima Industries Corp
Publication of EP3450451A1 publication Critical patent/EP3450451A1/en
Publication of EP3450451A4 publication Critical patent/EP3450451A4/en
Pending legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43563Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects
    • C07K14/43586Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from insects from silkworms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10Cells modified by introduction of foreign genetic material
    • DTEXTILES; PAPER
    • D01NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01FCHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F4/00Monocomponent artificial filaments or the like of proteins; Manufacture thereof
    • D01F4/02Monocomponent artificial filaments or the like of proteins; Manufacture thereof from fibroin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/20Fusion polypeptide containing a tag with affinity for a non-protein ligand

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Insects & Arthropods (AREA)
  • Toxicology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Textile Engineering (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Artificial Filaments (AREA)
EP17789711.3A 2016-04-28 2017-04-28 MODIFIED FIBROIN Pending EP3450451A4 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2016091201 2016-04-28
JP2016220741 2016-11-11
PCT/JP2017/016917 WO2017188430A1 (en) 2016-04-28 2017-04-28 Modified fibroin

Publications (2)

Publication Number Publication Date
EP3450451A1 EP3450451A1 (en) 2019-03-06
EP3450451A4 true EP3450451A4 (en) 2019-12-04

Family

ID=60159676

Family Applications (1)

Application Number Title Priority Date Filing Date
EP17789711.3A Pending EP3450451A4 (en) 2016-04-28 2017-04-28 MODIFIED FIBROIN

Country Status (10)

Country Link
US (1) US20190135881A1 (en)
EP (1) EP3450451A4 (en)
JP (1) JP6736801B2 (en)
KR (1) KR102242785B1 (en)
CN (1) CN109311954A (en)
AU (1) AU2017257938A1 (en)
BR (1) BR112018067895A2 (en)
CA (1) CA3012817A1 (en)
RU (1) RU2769982C2 (en)
WO (1) WO2017188430A1 (en)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2018159137A (en) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 Method for producing polypeptide fiber having spider-like structure
BR112018067899A2 (en) * 2016-04-28 2019-04-24 Spiber Inc. modified fibroin, nucleic acid, expression vector, host, and product.
JP2020097796A (en) * 2017-03-10 2020-06-25 Spiber株式会社 Artificial fibroin fiber
JP6337252B1 (en) * 2017-03-10 2018-06-06 Spiber株式会社 High shrinkage artificial fibroin fiber, method for producing the same, and method for shrinking artificial fibroin fiber
JP6807089B2 (en) * 2017-07-26 2021-01-06 Spiber株式会社 Modified fibroin
CN111655913A (en) * 2018-01-31 2020-09-11 丝芭博株式会社 Method for producing protein fiber
JP7174983B2 (en) * 2018-01-31 2022-11-18 Spiber株式会社 Spinning stock solution, fibroin fiber and method for producing the same
JP2021080573A (en) * 2018-01-31 2021-05-27 Spiber株式会社 Filamentary tow of protein filament and fabrication method thereof
EP3770317A4 (en) * 2018-03-22 2022-01-19 Shima Seiki Mfg., Ltd. Protein fiber crimping method, protein fiber production method, protein fibers, spun yarn, and textile product
JPWO2019194249A1 (en) * 2018-04-03 2021-04-15 Spiber株式会社 Doping liquid, modified fibroin fiber and its manufacturing method
EP3779000A4 (en) * 2018-04-03 2021-12-29 Spiber Inc. Composite fibers and method for manufacturing same
JP2021167277A (en) * 2018-04-03 2021-10-21 Spiber株式会社 Artificial fibroin fiber
EP3556914A1 (en) 2018-04-20 2019-10-23 Lenzing Aktiengesellschaft Regenerated cellulose moulded bodies and method for producing regenerated cellulose moulded bodies
JP2020121958A (en) * 2019-01-31 2020-08-13 Spiber株式会社 Protein molding manufacturing method
JP2020120642A (en) * 2019-01-31 2020-08-13 Spiber株式会社 Modified fibroin fiber manufacturing method and protein solution
JP2020120643A (en) * 2019-01-31 2020-08-13 Spiber株式会社 Modified fibroin fiber manufacturing method and protein solution
US20220251150A1 (en) * 2019-06-28 2022-08-11 Spiber Inc. Artificial Fur and Method for Manufacturing Same
JP7503287B2 (en) 2019-09-30 2024-06-20 Spiber株式会社 Flame Retardant Composition
WO2021153779A1 (en) * 2020-01-31 2021-08-05 Spiber株式会社 Meat substitute composition
AU2021289041A1 (en) * 2020-06-09 2023-01-19 Spiber Inc. Meat-like food composition
CN117881691A (en) * 2021-08-02 2024-04-12 丝芭博株式会社 Porous body and method for producing same
EP4410822A1 (en) 2021-11-02 2024-08-07 Spiber Inc. Esterified protein and production method thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0452925A2 (en) * 1990-04-20 1991-10-23 The University Of Wyoming Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the isolated DNA, and products thereof
US5994099A (en) * 1997-12-31 1999-11-30 The University Of Wyoming Extremely elastic spider silk protein and DNA coding therefor
WO2007025719A1 (en) * 2005-08-29 2007-03-08 Technische Universitaet Muenchen Modified spider silk proteins

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6608242B1 (en) * 2000-05-25 2003-08-19 E. I. Du Pont De Nemours And Company Production of silk-like proteins in plants
JP2005502347A (en) 2001-08-29 2005-01-27 ユニバーシティ オブ ワイオミング Nucleic acids, polypeptides, antibodies encoding spider silk proteins and methods for using them
WO2003056297A2 (en) * 2001-10-02 2003-07-10 Trustees Of Tufts College Self-assembling polymers, and materials fabricated therefrom
AU2003201513A1 (en) * 2002-01-11 2003-07-24 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
WO2011113446A1 (en) * 2010-03-17 2011-09-22 Amsilk Gmbh Method for production of polypeptide containing fibres
JP5771833B2 (en) 2010-09-10 2015-09-02 岡本株式会社 Nucleic acids, proteins encoded by nucleic acids, recombinant organisms into which nucleic acids have been introduced, and proteins made by recombinant organisms
CA2812791C (en) 2010-09-28 2020-07-14 The University Of Notre Dame Chimeric spider silk and uses thereof
WO2015042164A2 (en) 2013-09-17 2015-03-26 Refactored Materials, Inc. Methods and compositions for synthesizing improved silk fibers
BR112018067899A2 (en) * 2016-04-28 2019-04-24 Spiber Inc. modified fibroin, nucleic acid, expression vector, host, and product.

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0452925A2 (en) * 1990-04-20 1991-10-23 The University Of Wyoming Isolated DNA coding for spider silk protein, a replicable vector and a transformed cell containing the isolated DNA, and products thereof
US5994099A (en) * 1997-12-31 1999-11-30 The University Of Wyoming Extremely elastic spider silk protein and DNA coding therefor
WO2007025719A1 (en) * 2005-08-29 2007-03-08 Technische Universitaet Muenchen Modified spider silk proteins

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
See also references of WO2017188430A1 *

Also Published As

Publication number Publication date
EP3450451A1 (en) 2019-03-06
RU2018135565A3 (en) 2021-01-14
AU2017257938A1 (en) 2018-08-23
CN109311954A (en) 2019-02-05
CA3012817A1 (en) 2017-11-02
JPWO2017188430A1 (en) 2018-08-30
KR20180137529A (en) 2018-12-27
BR112018067895A2 (en) 2019-09-03
WO2017188430A1 (en) 2017-11-02
JP6736801B2 (en) 2020-08-05
RU2769982C2 (en) 2022-04-12
US20190135881A1 (en) 2019-05-09
RU2018135565A (en) 2020-05-28
KR102242785B1 (en) 2021-04-20

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