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EP3375523B1 - Microfluidic device - Google Patents

Microfluidic device Download PDF

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Publication number
EP3375523B1
EP3375523B1 EP18161894.3A EP18161894A EP3375523B1 EP 3375523 B1 EP3375523 B1 EP 3375523B1 EP 18161894 A EP18161894 A EP 18161894A EP 3375523 B1 EP3375523 B1 EP 3375523B1
Authority
EP
European Patent Office
Prior art keywords
microfluidic
card
component
assembly
magnetic effect
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
EP18161894.3A
Other languages
German (de)
French (fr)
Other versions
EP3375523A1 (en
Inventor
François BOIZOT
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/026—Fluid interfacing between devices or objects, e.g. connectors, inlet details
    • B01L2200/027—Fluid interfacing between devices or objects, e.g. connectors, inlet details for microfluidic devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0689—Sealing
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions

Definitions

  • the present invention relates to a microfluidic device.
  • This device comprises in particular a microfluidic card on which a microfluidic component is assembled using assembly means.
  • Microfluidic devices are well known and are used in particular in the field of research in biology, medicine or pharmacy. They allow fluidic interconnections to be made between several liquid or gaseous compounds. They can be in different forms, in particular in the form of a card having dimensions similar to those of a credit card.
  • the card thus comprises a microfluidic structure suitable for its application which may include various microfluidic elements. These microfluidic elements are for example input and output microfluidic connection points, microfluidic channels, chambers, microvalves, etc.
  • the card is for example positioned in a suitable housing for a controller which controls various operations, in particular the injection of the various fluids through the input connection points and their evacuation via the output connection points of the microfluidic structure of the card.
  • the microfluidic structure of the card In order to standardize the card and its microfluidic structure with respect to several applications, the microfluidic structure of the card must be able to be easily modified and adapted to the intended application. This adaptation must be carried out easily and without risk of malfunction, in particular by respecting the tightness of the device.
  • the aim of the invention is to provide a microfluidic device, the microfluidic structure of which can be easily modified and therefore adapted to a particular application.
  • microfluidic device as defined in claim 1.
  • the microfluidic card 2 is for example made from a plastic material such as PMMA (Polymethyl methacrylate), COC (“Cyclic Olefin Copolymer” - cycloolefin polymer) or other similar material.
  • PMMA Polymethyl methacrylate
  • COC Cyclic Olefin Copolymer
  • Microfluidic elements can in particular be formed on two depth levels of the card, being produced separately in the first layer 20 and in the second layer 21 of the card.
  • impressions are for example made by machining the surface of the corresponding layer.
  • microfluidic card 2 manufacture of such a microfluidic card 2 being well known, it is not detailed in the present application.
  • the card 2 thus formed has a so-called upper face 200, a so-called lower face 210 and a wafer 220 forming its thickness.
  • the microfluidic elements are formed in the thickness of the card 2 and accessible by inlet and outlet microfluidic connection points 201 which open out and which are accessible on the upper face 200 and / or on the lower face 210 of map 2.
  • the card also includes microfluidic channels 202 connecting different points of microfluidic connection 201 so as to form an array of interconnected microfluidic elements.
  • the card 2 On one of its faces, for example the upper face 200 (as shown in the figures 1 and 2 ), the card 2 comprises at least one bearing surface, called the first bearing surface, forming a specific location intended to receive a removable microfluidic component 3 as described below.
  • the microfluidic device 1 indeed comprises a microfluidic component 3 intended to be assembled on the card 2.
  • This component 3 comprises for example two opposite main faces, called the upper face and the lower face, separated from each other. by the thickness of the component, forming its edge.
  • This component has, for example, a general parallelepipedal shape. It also comprises on its underside a surface, called the second bearing surface, intended to come opposite the first bearing surface produced on the card 2 and along a plane parallel to this first bearing surface. .
  • This microfluidic component 3 also comprises a microfluidic structure, called the second microfluidic structure, composed of microfluidic elements such as those given as an example above.
  • the microfluidic component 3 has microfluidic connection points 311 and microfluidic channels 312 connecting the microfluidic connection points 311 so as to form an array of interconnected microfluidic elements.
  • microfluidic component 3 is removable with respect to the card 2.
  • the second microfluidic structure provided by the component 3 is connected to the first microfluidic structure of the card 2 via connection points available on the card and linked to the micro-fluidic structure of the card.
  • microfluidic connection points 201 of the card are connected to microfluidic connection points 311 of the component to interconnect the two microfluidic structures.
  • component 3 is removable, it can be replaced by a component 3 having the same microfluidic structure or chosen with a specific microfluidic structure according to the application for which the microfluidic device 1 is intended. invention.
  • the microfluidic component 3 of the device of the invention is in two parts. It comprises a first part 30 which carries means for assembling the component 3 on the card 2 and a second part 31 which comprises the microfluidic structure of the component.
  • the two parts 30, 31 can be made of identical or different materials.
  • the first part is arranged to hold the second part in position on the map.
  • the two-part architecture of component 3 advantageously allows it to separate its first part 30 dedicated to mechanical fixing from its second part 31 dedicated to the microfluidic application. It is thus possible to keep the first part 30 of the component 3 while modifying its microfluidic structure, simply by replacing its second part 31 with another having an identical or different microfluidic structure.
  • the first part 30 of the microfluidic component is, for example, in the form of a frame of rectangular shape having an upper surface and a lower surface.
  • the second part 31 of the microfluidic component 3 is, for example, in the form of a microfluidic plate or chip coming to rest against the lower surface of the frame formed by the first part 30 so as to be held by the latter.
  • This microfluidic chip is for example made from a material such as silicon. Its microfluidic structure is created by known processes.
  • the microfluidic device 1 of the invention comprises at least one seal 4, advantageously flat, intended to be positioned between the first bearing surface and the second bearing surface described above, so as to produce the seal between the card 2 and the component 3.
  • this seal is sandwiched between the plate or chip formed by the second part 31 of the micro component. fluidics 3 and the upper face 200 of the microfluidic card 2.
  • this seal is for example composed of a strip or a plate, made of a flexible material such as for example rubber or silicone.
  • the silicone seal has the particularity of adhering very well to a silicon surface such as that which makes up the chip of the second part 31 of the microfluidic component 3.
  • such a silicone seal can be repositioned at will. on the silicon chip.
  • the seal 4 comprises several orifices 40 made through the thickness of the strip and each intended to be positioned in the axis of a microfluidic connection point 201 of the card and of a micro connection point. -fluidic 311 of the component so as to ensure the sealing of the connection between the two points and therefore, more generally, between the microfluidic structure of the card 2 and the microfluidic structure of the component 3 when the two structures are interconnected.
  • the device 1 comprises for example two seals of this type for each microfluidic component 3 in order to seal the microfluidic inlet connection points and the microfluidic connection points. Release. These may be identical or different, in terms of dimensions or in the position of the orifices ...
  • each seal is positioned in a suitable manner to ensure the watertight junction of several connection points at the same time using a single gasket.
  • Each seal is advantageously fixed on the upper face 200 of the microfluidic card 2 or on the face facing the microfluidic component 3.
  • the seal is flat and comprises, on one of its two flat faces, a bead 41 or extra thickness produced in the direction of the thickness of the seal around each orifice 40 and against which comes apply compression.
  • the seal 4 can be made integral with the microfluidic card 2 or with the microfluidic component 3, before the component 3 is mounted on the card 2.
  • the device 1 comprises means for centering and assembling the microfluidic component 3 on the microfluidic card 2. These centering and assembly means are arranged to ensure sufficient compression of the seal 4. These means comprise at least one magnetic device.
  • the centering and assembly means also comprise a mechanical device cooperating with said magnetic device to ensure the centering and assembly of the component on the card.
  • the magnetic device thus integrates at least two assembly elements by magnetic effect arranged to attract each other by magnetic effect.
  • the mechanical device preferably comprises an assembly solution of the male / female type. It thus comprises, for example, pads 303 and corresponding cavities 203.
  • said pads 303 are for example made on the microfluidic component 3 while the cavities are formed on the microfluidic card 2.
  • Said cavities 203 are arranged on the upper face 200 of the card, around the first bearing surface, and are each intended to accommodate a corresponding pad 303 produced on component 3.
  • pads 303 are arranged to position and hold the second part of the component in position on the card 2
  • the pads 303 are advantageously formed on the first part 30 of the component 3 and are distributed over the second bearing surface of the microfluidic component 3. This second bearing surface is produced in whole or in part on the second part 31 of the.
  • the second part 31 of the microfluidic component 3 comprises the microfluidic structure of the component 3, that is to say in particular the microfluidic connection points 311 of inlet and / or outlet and the microfluidic channels 312 mentioned above.
  • these microfluidic connection points 311 are intended to be connected to the inlet and / or outlet microfluidic connection points 201 made on the board 2 to connect the microfluidic structure of component 3 to the microfluidic structure of the card 2.
  • the pads 303 are for example in the form of a cylinder of revolution and the cavities 203 have a corresponding female shape.
  • the magnetic device comprises several assembly elements by magnetic effect such as permanent magnets 50, each forming, by their shape, a pad 303 of the mechanical device or housed in a pad 303 of the mechanical device to associate the pad with a magnetic effect.
  • the magnetic device also comprises several other assembly elements by magnetic effect, for example also permanent magnets 60, which are advantageously housed in the thickness of the card, so as to present a face in the bottom of each cavity 203 or separate. from the bottom of said cavity 203 by a card thickness at this location.
  • These permanent magnets 60 are for example housed in spaces formed in the second layer 21 of the microfluidic card 2, while the cavities 203 are formed by orifices which may or may not pass through the first layer 20 of the card. According to this particular embodiment, the permanent magnets 50 are positioned at the level of the four corners of the component. Each cavity 203 may in fact be blind through the first layer 20. Thus, each permanent magnet 60 is trapped in the second layer 21 of the card 2 once the two layers 20, 21 are assembled one on top of the other. 'other.
  • an air gap (referenced e on the figure 3B ) is advantageously left between each permanent magnet 50 of the component and the corresponding permanent magnet 60 of the microfluidic card 2 (or more generally between two assembly elements by magnetic effect).
  • each cavity 203 with a depth greater than the height of each corresponding pad 303 of the component.
  • the presence of air gaps has the advantage of ensuring uniform compression of the seal 4 by the magnetic effect.
  • the air gap can be produced at least by the thickness of material present between the bottom of the cavity 203 made in the first layer 20 and the magnet 60 present below in the second layer 21.
  • the bead 41 produced on the surface of the seal around each of its orifices 40 makes it possible to ensure the exercise of a maximum force on the surface of the bead 41 by the magnetic effect.
  • microfluidic card 2 is shown with two separate locations for accommodating two components of the type described above on the same card.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Micromachines (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)

Description

Domaine technique de l'inventionTechnical field of the invention

La présente invention se rapporte à un dispositif micro-fluidique. Ce dispositif comporte notamment une carte micro-fluidique sur laquelle est assemblé un composant micro-fluidique à l'aide de moyens d'assemblage.The present invention relates to a microfluidic device. This device comprises in particular a microfluidic card on which a microfluidic component is assembled using assembly means.

Etat de la techniqueState of the art

Les dispositifs micro-fluidiques sont bien connus et sont notamment employés dans le domaine de la recherche en biologie, en médecine ou en pharmacie. Ils permettent de réaliser des interconnexions fluidiques entre plusieurs composés liquides ou gazeux. Ils peuvent se présenter sous différentes formes, notamment sous la forme d'une carte ayant des dimensions similaires à celles d'une carte de crédit. La carte comporte ainsi une structure micro-fluidique adaptée à son application qui peut comporter différents éléments micro-fluidiques. Ces éléments micro-fluidiques sont par exemple des points de connexion micro-fluidique d'entrée et de sortie, des canaux micro-fluidiques, des chambres, des micro-vannes,... La carte est par exemple positionnée dans un logement adapté d'un automate qui commande différentes opérations, notamment l'injection des différents fluides à travers les points de connexion d'entrée et leur évacuation par les points de connexion de sortie de la structure micro-fluidique de la carte.Microfluidic devices are well known and are used in particular in the field of research in biology, medicine or pharmacy. They allow fluidic interconnections to be made between several liquid or gaseous compounds. They can be in different forms, in particular in the form of a card having dimensions similar to those of a credit card. The card thus comprises a microfluidic structure suitable for its application which may include various microfluidic elements. These microfluidic elements are for example input and output microfluidic connection points, microfluidic channels, chambers, microvalves, etc. The card is for example positioned in a suitable housing for a controller which controls various operations, in particular the injection of the various fluids through the input connection points and their evacuation via the output connection points of the microfluidic structure of the card.

Afin de standardiser la carte et sa structure micro-fluidique par rapport à plusieurs applications, la structure micro-fluidique de la carte doit pouvoir être modifiée facilement et adaptée à l'application visée. Cette adaptation doit être réalisée facilement et sans risque de dysfonctionnement, en respectant notamment l'étanchéité du dispositif.In order to standardize the card and its microfluidic structure with respect to several applications, the microfluidic structure of the card must be able to be easily modified and adapted to the intended application. This adaptation must be carried out easily and without risk of malfunction, in particular by respecting the tightness of the device.

La demande de brevet US2010/322826A1 décrit un principe de connexion micro-fluidique par effet magnétique. Ce document est simplement limité à ce principe de connexion magnétique et la solution proposée n'est pas forcément applicable à un dispositif micro-fluidique qui comporte à la fois une carte micro-fluidique et un composant micro-fluidique indépendant et adaptable sur la carte.The patent application US2010 / 322826A1 describes a principle of microfluidic connection by magnetic effect. This document is simply limited to this principle of magnetic connection and the proposed solution is not necessarily applicable to a microfluidic device which comprises both a microfluidic card and an independent microfluidic component which can be adapted to the card.

Le but de l'invention est de proposer un dispositif micro-fluidique dont la structure micro-fluidique peut être modifiée aisément et donc adaptée à une application particulière.The aim of the invention is to provide a microfluidic device, the microfluidic structure of which can be easily modified and therefore adapted to a particular application.

Exposé de l'inventionDisclosure of the invention

Ce but est atteint par un dispositif micro-fluidique tel que défini en revendication 1.This object is achieved by a microfluidic device as defined in claim 1.

Des particularités du dispositif sont définies dans les revendications secondaires 2 à 6.Features of the device are defined in secondary claims 2 to 6.

Brève description des figuresBrief description of the figures

D'autres caractéristiques et avantages vont apparaître dans la description détaillée qui suit, faite en regard des dessins annexés dans lesquels :

  • La figure 1 représente, vu en éclaté et en perspective, le dispositif micro-fluidique de l'invention.
  • La figure 2 représente, vu de dessus et assemblé, le dispositif micro-fluidique de l'invention.
  • Les figure 3A et 3B représentent, vu suivant une coupe transversale, le dispositif micro-fluidique de l'invention, respectivement avec le composant micro-fluidique non assemblé sur la carte micro-fluidique et avec le composant micro-fluidique assemblé sur la carte micro-fluidique.
  • Les figure 4A et 4B représentent, respectivement en vue de dessus et en vue de côté, le joint plat employé dans le dispositif micro-fluidique de l'invention.
Other characteristics and advantages will appear in the detailed description which follows, given with reference to the appended drawings in which:
  • The figure 1 shows, exploded and in perspective, the microfluidic device of the invention.
  • The figure 2 shows, seen from above and assembled, the microfluidic device of the invention.
  • The figure 3A and 3B represent, seen in a cross section, the microfluidic device of the invention, respectively with the microfluidic component not assembled on the microfluidic card and with the microfluidic component assembled on the microfluidic card.
  • The figure 4A and 4B represent, respectively in top view and in side view, the flat seal used in the microfluidic device of the invention.

Sur les figures annexées, les chemins fluidiques sont représentés de manière simplifiée et n'ont pas forcément de correspondance avec la réalité.In the appended figures, the fluidic paths are shown in a simplified manner and do not necessarily correspond to reality.

Description détaillée d'au moins un mode de réalisationDetailed description of at least one embodiment

Dans la suite de la description, les termes "inférieur" et "supérieur" sont à comprendre en prenant comme référence un axe vertical.In the remainder of the description, the terms “lower” and “upper” are to be understood by taking a vertical axis as a reference.

En référence à la figure 1, le dispositif micro-fluidique 1 comporte une carte micro-fluidique 2. Cette carte 2 a par exemple les dimensions d'une carte de crédit. Elle comporte une structure micro-fluidique, dite première structure micro-fluidique, qui comporte plusieurs éléments micro-fluidiques. Par éléments micro-fluidiques, on entend par exemple :

  • des points de connexion micro-fluidique d'entrée par lesquels peut être injecté un fluide dans la structure micro-micro-fluidique ;
  • des points de connexion micro-fluidique de sortie par lesquels peut être extrait un fluide de la structure micro-fluidique ;
  • des canaux micro-fluidiques formant un réseau plus ou moins complexe entre un ou plusieurs points de connexion micro-fluidique d'entrée et un ou plusieurs points de connexion micro-fluidique sortie ;
  • des chambres de réaction ou de stockage ;
  • des micro-vannes...
With reference to the figure 1 , the microfluidic device 1 comprises a microfluidic card 2. This card 2 has for example the dimensions of a credit card. It comprises a microfluidic structure, called the first microfluidic structure, which comprises several microfluidic elements. By microfluidic elements is meant for example:
  • inlet microfluidic connection points through which a fluid can be injected into the microfluidic structure;
  • outlet microfluidic connection points through which fluid can be extracted from the microfluidic structure;
  • microfluidic channels forming a more or less complex network between one or more inlet microfluidic connection points and one or more outlet microfluidic connection points;
  • reaction or storage chambers;
  • micro-valves ...

La carte micro-fluidique 2 est par exemple réalisée dans un matériau plastique tel que le PMMA (Polyméthacrylate de Méthyle), le COC ("Cyclic Olefin Copolymer" - polymère de cyclooléfine) ou autre matériau similaire.The microfluidic card 2 is for example made from a plastic material such as PMMA (Polymethyl methacrylate), COC (“Cyclic Olefin Copolymer” - cycloolefin polymer) or other similar material.

De manière non limitative, la carte micro-fluidique est réalisée par assemblage d'au moins deux couches 20, 21 collées sur l'une l'autre et dites première couche 20 et deuxième couche 21, visibles sur la figure 1. De manière non limitative, les éléments micro-fluidiques définis ci-dessus peuvent être formés de différentes manières, comme par exemple :

  • Sous la forme d'une empreinte réalisée sur la surface d'une couche qui est ensuite recouverte par l'autre couche ;
  • En superposant une première empreinte réalisée sur la surface de la première couche et une deuxième empreinte réalisée sur la surface de la deuxième couche, la première empreinte étant située en vis-à-vis de la deuxième empreinte pour former l'élément fluidique.
In a nonlimiting manner, the microfluidic card is produced by assembling at least two layers 20, 21 bonded to one another and called first layer 20 and second layer 21, visible on the figure 1 . In a nonlimiting manner, the microfluidic elements defined above can be formed in different ways, such as for example:
  • In the form of an imprint made on the surface of one layer which is then covered by the other layer;
  • By superimposing a first impression made on the surface of the first layer and a second impression made on the surface of the second layer, the first impression being situated opposite the second impression to form the fluidic element.

Des éléments micro-fluidiques peuvent notamment être formés sur deux niveaux de profondeur de la carte, en étant réalisés de manière distincte dans la première couche 20 et dans la deuxième couche 21 de la carte.Microfluidic elements can in particular be formed on two depth levels of the card, being produced separately in the first layer 20 and in the second layer 21 of the card.

Les empreintes sont par exemple réalisées par usinage de la surface de la couche correspondante.The impressions are for example made by machining the surface of the corresponding layer.

La fabrication d'une telle carte micro-fluidique 2 étant bien connue, celle-ci n'est pas détaillée dans la présente demande.The manufacture of such a microfluidic card 2 being well known, it is not detailed in the present application.

La carte 2 ainsi formée comporte une face dite supérieure 200, une face dite inférieure 210 et une tranche 220 formant son épaisseur. Les éléments micro-fluidiques sont formés dans l'épaisseur de la carte 2 et accessibles par des points de connexion micro-fluidique 201 d'entrée et de sortie qui débouchent et qui sont accessibles sur la face supérieure 200 et/ou sur la face inférieure 210 de la carte 2. Sur les figures 1 et 2, la carte comporte également des canaux micro-fluidiques 202 reliant différents points de connexion micro-fluidique 201 de manière à former un réseau d'éléments micro-fluidiques interconnectés.The card 2 thus formed has a so-called upper face 200, a so-called lower face 210 and a wafer 220 forming its thickness. The microfluidic elements are formed in the thickness of the card 2 and accessible by inlet and outlet microfluidic connection points 201 which open out and which are accessible on the upper face 200 and / or on the lower face 210 of map 2. On the figures 1 and 2 , the card also includes microfluidic channels 202 connecting different points of microfluidic connection 201 so as to form an array of interconnected microfluidic elements.

Sur l'une de ses faces, par exemple la face supérieure 200 (comme représenté sur les figures 1 et 2), la carte 2 comporte au moins une surface d'appui, dite première surface d'appui formant un emplacement spécifique destiné à recevoir un composant micro-fluidique 3 amovible tel que décrit ci-dessous.On one of its faces, for example the upper face 200 (as shown in the figures 1 and 2 ), the card 2 comprises at least one bearing surface, called the first bearing surface, forming a specific location intended to receive a removable microfluidic component 3 as described below.

Selon l'invention, le dispositif micro-fluidique 1 comporte en effet un composant micro-fluidique 3 destiné à être assemblé sur la carte 2. Ce composant 3 comporte par exemple deux faces principales opposées, dites face supérieure et face inférieure, séparées entre elles par l'épaisseur du composant, formant sa tranche. Ce composant présente par exemple une forme générale parallélépipédique. Il comporte également sur sa face inférieure une surface, dite deuxième surface d'appui, destinée à venir en vis-à-vis de la première surface d'appui réalisée sur la carte 2 et suivant un plan parallèle à cette première surface d'appui.According to the invention, the microfluidic device 1 indeed comprises a microfluidic component 3 intended to be assembled on the card 2. This component 3 comprises for example two opposite main faces, called the upper face and the lower face, separated from each other. by the thickness of the component, forming its edge. This component has, for example, a general parallelepipedal shape. It also comprises on its underside a surface, called the second bearing surface, intended to come opposite the first bearing surface produced on the card 2 and along a plane parallel to this first bearing surface. .

Ce composant micro-fluidique 3 comporte également une structure micro-fluidique, dite deuxième structure micro-fluidique, composé d'éléments micro-fluidiques tels que ceux donnés en exemple ci-dessus. Sur les figures 1 et 2, le composant micro-fluidique 3 comporte des points de connexion micro-fluidique 311 et des canaux micro-fluidiques 312 reliant les points de connexion micro-fluidique 311 de manière à former un réseau d'éléments micro-fluidiques interconnectés.This microfluidic component 3 also comprises a microfluidic structure, called the second microfluidic structure, composed of microfluidic elements such as those given as an example above. On the figures 1 and 2 , the microfluidic component 3 has microfluidic connection points 311 and microfluidic channels 312 connecting the microfluidic connection points 311 so as to form an array of interconnected microfluidic elements.

Ce composant micro-fluidique 3 est amovible par rapport à la carte 2. Lorsque le composant 3 est assemblé sur la carte 2, la deuxième structure micro-fluidique apportée par le composant 3 est connectée à la première structure micro-fluidique de la carte 2 par l'intermédiaire de points de connexion disponibles sur la carte et reliés à la structure micro-fluidique de la carte. Ainsi des points de connexion micro-fluidique 201 de la carte sont connectés à des points de connexion micro-fluidique 311 du composant pour interconnecter les deux structures micro-fluidiques.This microfluidic component 3 is removable with respect to the card 2. When the component 3 is assembled on the card 2, the second microfluidic structure provided by the component 3 is connected to the first microfluidic structure of the card 2 via connection points available on the card and linked to the micro-fluidic structure of the card. Thus, microfluidic connection points 201 of the card are connected to microfluidic connection points 311 of the component to interconnect the two microfluidic structures.

Comme le composant 3 est amovible, celui-ci peut être remplacé par un composant 3 ayant la même structure micro-fluidique ou choisi avec une structure micro-fluidique spécifique en fonction de l'application à laquelle est destiné le dispositif micro-fluidique 1 de l'invention.As component 3 is removable, it can be replaced by a component 3 having the same microfluidic structure or chosen with a specific microfluidic structure according to the application for which the microfluidic device 1 is intended. invention.

Avantageusement, le composant micro-fluidique 3 du dispositif de l'invention se présente en deux parties. Il comporte une première partie 30 qui porte des moyens d'assemblage du composant 3 sur la carte 2 et une deuxième partie 31 qui comporte la structure micro-fluidique du composant. Les deux parties 30, 31 peuvent être réalisées dans des matériaux identiques ou différents. La première partie est agencée pour maintenir la deuxième partie en position sur la carte.Advantageously, the microfluidic component 3 of the device of the invention is in two parts. It comprises a first part 30 which carries means for assembling the component 3 on the card 2 and a second part 31 which comprises the microfluidic structure of the component. The two parts 30, 31 can be made of identical or different materials. The first part is arranged to hold the second part in position on the map.

L'architecture en deux parties du composant 3 lui permet avantageusement de séparer sa première partie 30 dédiée à la fixation mécanique de sa deuxième partie 31 dédiée à l'application micro-fluidique. Il est ainsi possible de conserver la première partie 30 du composant 3 tout en modifiant sa structure micro-fluidique, simplement en remplaçant sa deuxième partie 31 par une autre ayant une structure micro-fluidique identique ou différente.The two-part architecture of component 3 advantageously allows it to separate its first part 30 dedicated to mechanical fixing from its second part 31 dedicated to the microfluidic application. It is thus possible to keep the first part 30 of the component 3 while modifying its microfluidic structure, simply by replacing its second part 31 with another having an identical or different microfluidic structure.

La première partie 30 du composant micro-fluidique se présente par exemple sous la forme d'un cadre de forme rectangulaire présentant une surface supérieure et une surface inférieure. La deuxième partie 31 du composant micro-fluidique 3 se présente par exemple sous la forme d'une plaque ou puce micro-fluidique venant se positionner en appui contre la surface inférieure du cadre formé par la première partie 30 de manière être maintenue par celle-ci en vis-à-vis de la face supérieure 200 de la carte micro-fluidique 2. Cette puce micro-fluidique est par exemple réalisée dans un matériau comme le silicium. Sa structure micro-fluidique est créée par des processus connus.The first part 30 of the microfluidic component is, for example, in the form of a frame of rectangular shape having an upper surface and a lower surface. The second part 31 of the microfluidic component 3 is, for example, in the form of a microfluidic plate or chip coming to rest against the lower surface of the frame formed by the first part 30 so as to be held by the latter. Ci facing the upper face 200 of the microfluidic card 2. This microfluidic chip is for example made from a material such as silicon. Its microfluidic structure is created by known processes.

Le dispositif micro-fluidique 1 de l'invention comporte au moins un joint d'étanchéité 4, avantageusement plat, destiné à être positionné entre la première surface d'appui et la deuxième surface d'appui décrites ci-dessus, de manière à réaliser l'étanchéité entre la carte 2 et le composant 3. Dans l'architecture décrite ci-dessus et représentée sur les figures, ce joint d'étanchéité est pris en sandwich entre la plaque ou puce formée par la deuxième partie 31 du composant micro-fluidique 3 et la face supérieure 200 de la carte micro-fluidique 2.The microfluidic device 1 of the invention comprises at least one seal 4, advantageously flat, intended to be positioned between the first bearing surface and the second bearing surface described above, so as to produce the seal between the card 2 and the component 3. In the architecture described above and shown in the figures, this seal is sandwiched between the plate or chip formed by the second part 31 of the micro component. fluidics 3 and the upper face 200 of the microfluidic card 2.

Comme représenté sur les figures 4A et 4B, ce joint d'étanchéité se compose par exemple d'une bande ou d'une plaque, réalisée en matériau souple tel que par exemple du caoutchouc ou de la silicone. De manière avantageuse, le joint en silicone présente la particularité de très bien adhérer à une surface en silicium comme celle qui compose la puce de la deuxième partie 31 du composant micro-fluidique 3. De plus, un tel joint en silicone est repositionnable à volonté sur la puce en silicium.As shown on figures 4A and 4B , this seal is for example composed of a strip or a plate, made of a flexible material such as for example rubber or silicone. Advantageously, the silicone seal has the particularity of adhering very well to a silicon surface such as that which makes up the chip of the second part 31 of the microfluidic component 3. In addition, such a silicone seal can be repositioned at will. on the silicon chip.

Le joint d'étanchéité 4 comporte plusieurs orifices 40 réalisés à travers l'épaisseur de la bande et destinés chacun à se positionner dans l'axe d'un point de connexion micro-fluidique 201 de la carte et d'un point de connexion micro-fluidique 311 du composant de manière à assurer l'étanchéité de la liaison entre les deux points et donc, de manière plus générale, entre la structure micro-fluidique de la carte 2 et la structure micro-fluidique du composant 3 lorsque les deux structures sont interconnectées. Comme représenté sur les figures annexées, le dispositif 1 comporte par exemple deux joints d'étanchéité de ce type pour chaque composant micro-fluidique 3 afin d'étanchéifier des points de connexion micro-fluidique d'entrée et des points de connexion micro-fluidique de sortie. Ceux-ci pourront être identiques ou différents, en termes de dimensions ou dans la position des orifices... Les orifices réalisés à travers chaque joint d'étanchéité sont positionnés de manière adaptée pour assurer la jonction étanche de plusieurs points de connexion en même temps à l'aide d'un seul joint d'étanchéité. Chaque joint est avantageusement fixé sur la face supérieure 200 de la carte micro-fluidique 2 ou sur la face en vis-à-vis du composant micro-fluidique 3.The seal 4 comprises several orifices 40 made through the thickness of the strip and each intended to be positioned in the axis of a microfluidic connection point 201 of the card and of a micro connection point. -fluidic 311 of the component so as to ensure the sealing of the connection between the two points and therefore, more generally, between the microfluidic structure of the card 2 and the microfluidic structure of the component 3 when the two structures are interconnected. As shown in the accompanying figures, the device 1 comprises for example two seals of this type for each microfluidic component 3 in order to seal the microfluidic inlet connection points and the microfluidic connection points. Release. These may be identical or different, in terms of dimensions or in the position of the orifices ... The orifices made through each seal are positioned in a suitable manner to ensure the watertight junction of several connection points at the same time using a single gasket. Each seal is advantageously fixed on the upper face 200 of the microfluidic card 2 or on the face facing the microfluidic component 3.

Selon un aspect avantageux de l'invention, le joint est plat et comporte, sur l'une de ses deux faces planes, un bourrelet 41 ou surépaisseur réalisée dans le sens de l'épaisseur du joint autour de chaque orifice 40 et contre lequel vient s'appliquer la compression.According to an advantageous aspect of the invention, the seal is flat and comprises, on one of its two flat faces, a bead 41 or extra thickness produced in the direction of the thickness of the seal around each orifice 40 and against which comes apply compression.

Selon un aspect de l'invention, le joint d'étanchéité 4 peut être rendu solidaire de la carte micro-fluidique 2 ou du composant micro-fluidique 3, avant montage du composant 3 sur la carte 2.According to one aspect of the invention, the seal 4 can be made integral with the microfluidic card 2 or with the microfluidic component 3, before the component 3 is mounted on the card 2.

Le dispositif 1 comporte des moyens de centrage et d'assemblage du composant micro-fluidique 3 sur la carte micro-fluidique 2. Ces moyens de centrage et d'assemblage sont agencés pour assurer une mise en compression suffisante du joint 4. Ces moyens comportent au moins un dispositif magnétique.The device 1 comprises means for centering and assembling the microfluidic component 3 on the microfluidic card 2. These centering and assembly means are arranged to ensure sufficient compression of the seal 4. These means comprise at least one magnetic device.

De manière avantageuse, les moyens de centrage et d'assemblage comportent également un dispositif mécanique coopérant avec ledit dispositif magnétique pour assurer le centrage et l'assemblage du composant sur la carte.Advantageously, the centering and assembly means also comprise a mechanical device cooperating with said magnetic device to ensure the centering and assembly of the component on the card.

Le dispositif magnétique intègre ainsi au moins deux éléments d'assemblage par effet magnétique agencés pour s'attirer par effet magnétique.The magnetic device thus integrates at least two assembly elements by magnetic effect arranged to attract each other by magnetic effect.

Par élément d'assemblage par effet magnétique, on entend un aimant permanent et/ou une pièce en matériau ferromagnétique ou une combinaison des deux, au moins l'un des deux éléments étant un aimant permanent pour assurer un phénomène d'attraction magnétique entre le composant micro-fluidique 3 et la carte micro-fluidique 2. Ces deux éléments sont répartis entre la carte micro-fluidique 2 et le composant micro-fluidique 3 pour assurer l'assemblage. Une polarisation magnétique adaptée du dispositif magnétique permet en outre d'assurer un centrage par effet magnétique. L'effet magnétique généré entre deux éléments d'assemblage par effet magnétique est choisi suffisant pour :

  • Maintenir le composant micro-fluidique 3 dans sa position sur la carte 2, et
  • Assurer une compression de chaque joint 4 et permettre ainsi une connexion fluidique étanche entre la structure micro-fluidique de la carte 2 et la structure micro-fluidique du composant 3.
By magnetic effect assembly element means a permanent magnet and / or a part made of ferromagnetic material or a combination of the two, at least one of the two elements being a permanent magnet to ensure a phenomenon of magnetic attraction between the microfluidic component 3 and the microfluidic card 2. These two elements are distributed between the microfluidic card 2 and the microfluidic component 3 to ensure assembly. A suitable magnetic polarization of the magnetic device also makes it possible to ensure centering by effect. magnetic. The magnetic effect generated between two assembly elements by magnetic effect is chosen sufficient for:
  • Maintain the microfluidic component 3 in its position on the card 2, and
  • Ensure compression of each seal 4 and thus allow a tight fluidic connection between the microfluidic structure of card 2 and the microfluidic structure of component 3.

Le dispositif mécanique comporte préférentiellement une solution d'assemblage de type mâle/femelle. Il comporte ainsi par exemple des plots 303 et des cavités 203 correspondantes. De manière non limitative, lesdits plots 303 sont par exemple réalisés sur le composant micro-fluidique 3 tandis que les cavités sont formées sur la carte micro-fluidique 2. Lesdites cavités 203 sont agencées sur la face supérieure 200 de la carte, autour de la première surface d'appui, et sont destinées chacune à accueillir un plot 303 correspondant réalisé sur le composant 3. Sur le composant micro-fluidique 3, les plots 303 sont agencés pour positionner et maintenir la deuxième partie du composant en position sur la carte 2. Les plots 303 sont avantageusement formés sur la première partie 30 du composant 3 et sont répartis sur la deuxième surface d'appui du composant micro-fluidique 3. Cette deuxième surface d'appui est réalisée en tout ou partie sur la deuxième partie 31 du composant. La deuxième partie 31 du composant micro-fluidique 3 comporte la structure micro-fluidique du composant 3, c'est-à-dire notamment les points de connexion micro-fluidique 311 d'entrée et/ou de sortie et les canaux micro-fluidiques 312 évoqués ci-dessus. Comme déjà évoqué, ces points de connexion micro-fluidique 311 sont destinés à se connecter sur des points de connexion micro-fluidique 201 d'entrée et/ou de sortie réalisés sur la carte 2 pour connecter la structure micro-fluidique du composant 3 à la structure micro-fluidique de la carte 2. Les plots 303 sont par exemple en forme de cylindre de révolution et les cavités 203 disposent d'une forme femelle correspondante.The mechanical device preferably comprises an assembly solution of the male / female type. It thus comprises, for example, pads 303 and corresponding cavities 203. In a non-limiting manner, said pads 303 are for example made on the microfluidic component 3 while the cavities are formed on the microfluidic card 2. Said cavities 203 are arranged on the upper face 200 of the card, around the first bearing surface, and are each intended to accommodate a corresponding pad 303 produced on component 3. On microfluidic component 3, pads 303 are arranged to position and hold the second part of the component in position on the card 2 The pads 303 are advantageously formed on the first part 30 of the component 3 and are distributed over the second bearing surface of the microfluidic component 3. This second bearing surface is produced in whole or in part on the second part 31 of the. component. The second part 31 of the microfluidic component 3 comprises the microfluidic structure of the component 3, that is to say in particular the microfluidic connection points 311 of inlet and / or outlet and the microfluidic channels 312 mentioned above. As already mentioned, these microfluidic connection points 311 are intended to be connected to the inlet and / or outlet microfluidic connection points 201 made on the board 2 to connect the microfluidic structure of component 3 to the microfluidic structure of the card 2. The pads 303 are for example in the form of a cylinder of revolution and the cavities 203 have a corresponding female shape.

Selon un mode de réalisation particulier illustré par les figures 3A et 3B, le dispositif magnétique comporte plusieurs éléments d'assemblage par effet magnétique tels que des aimants permanents 50, formant chacun, par leur forme, un plot 303 du dispositif mécanique ou logé dans un plot 303 du dispositif mécanique pour associer au plot un effet magnétique. Le dispositif magnétique comporte aussi plusieurs autres éléments d'assemblage par effet magnétique, par exemple aussi des aimants permanents 60, qui sont avantageusement logés dans l'épaisseur de la carte, de manière à présenter une face dans le fond de chaque cavité 203 ou séparée du fond de ladite cavité 203 par une épaisseur de carte à cet endroit. Ces aimants permanents 60 sont par exemple logés dans des espaces ménagés dans la deuxième couche 21 de la carte micro-fluidique 2 alors que les cavités 203 sont réalisées par des orifices qui traversent ou non la première couche 20 de la carte. Selon ce mode de réalisation particulier, les aimants permanents 50 sont positionnés au niveau des quatre coins du composant. Chaque cavité 203 peut en effet être non traversante à travers la première couche 20. Ainsi, chaque aimant permanent 60 se trouve emprisonné dans la deuxième couche 21 de la carte 2 une fois que les deux couches 20, 21 sont assemblées l'une sur l'autre.According to a particular embodiment illustrated by the figures 3A and 3B , the magnetic device comprises several assembly elements by magnetic effect such as permanent magnets 50, each forming, by their shape, a pad 303 of the mechanical device or housed in a pad 303 of the mechanical device to associate the pad with a magnetic effect. The magnetic device also comprises several other assembly elements by magnetic effect, for example also permanent magnets 60, which are advantageously housed in the thickness of the card, so as to present a face in the bottom of each cavity 203 or separate. from the bottom of said cavity 203 by a card thickness at this location. These permanent magnets 60 are for example housed in spaces formed in the second layer 21 of the microfluidic card 2, while the cavities 203 are formed by orifices which may or may not pass through the first layer 20 of the card. According to this particular embodiment, the permanent magnets 50 are positioned at the level of the four corners of the component. Each cavity 203 may in fact be blind through the first layer 20. Thus, each permanent magnet 60 is trapped in the second layer 21 of the card 2 once the two layers 20, 21 are assembled one on top of the other. 'other.

De manière avantageuse, lorsque le composant micro-fluidique 3 est assemblé sur la carte micro-fluidique 2 grâce aux deux dispositifs mécanique et magnétique décrits ci-dessus, un entrefer (référencé e sur la figure 3B) est avantageusement laissé entre chaque aimant permanent 50 du composant et l'aimant permanent 60 correspondant de la carte micro-fluidique 2 (ou de manière plus générale entre deux éléments d'assemblage par effet magnétique). Pour cela, Il s'agit par exemple de réaliser chaque cavité 203 avec une profondeur supérieure à la hauteur de chaque plot 303 correspondant du composant. La présence d'entrefers présente l'avantage d'assurer une compression uniforme du joint 4 par l'effet magnétique. L'entrefer peut être réalisé au moins par l'épaisseur de matière présente entre le fond de la cavité 203 réalisée dans la première couche 20 et l'aimant 60 présent au-dessous dans la deuxième couche 21.Advantageously, when the microfluidic component 3 is assembled on the microfluidic card 2 by virtue of the two mechanical and magnetic devices described above, an air gap (referenced e on the figure 3B ) is advantageously left between each permanent magnet 50 of the component and the corresponding permanent magnet 60 of the microfluidic card 2 (or more generally between two assembly elements by magnetic effect). For this, it is for example a matter of making each cavity 203 with a depth greater than the height of each corresponding pad 303 of the component. The presence of air gaps has the advantage of ensuring uniform compression of the seal 4 by the magnetic effect. The air gap can be produced at least by the thickness of material present between the bottom of the cavity 203 made in the first layer 20 and the magnet 60 present below in the second layer 21.

De manière avantageuse, le bourrelet 41 réalisé sur la surface du joint autour de chacun de ses orifices 40 permet d'assurer l'exercice d'une force maximale sur la surface du bourrelet 41 par l'effet magnétique.Advantageously, the bead 41 produced on the surface of the seal around each of its orifices 40 makes it possible to ensure the exercise of a maximum force on the surface of the bead 41 by the magnetic effect.

De manière non limitative, ci-dessous quelques éléments de dimensionnement des moyens de centrage et d'assemblage décrits ci-dessus :

  • Aimant composant de type Néodyme : forme cylindrique - diamètre de 3mm - hauteur 3mm - force d'adhérence de 290g.
  • Aimant carte de type Néodyme : forme parallélépipédique - dimensions 6x5x2mm - force d'adhérence de 600g.
  • Entrefer entre les deux aimants : environ 1mm.
In a non-limiting manner, below are some elements for dimensioning the centering and assembly means described above:
  • Neodymium component magnet: cylindrical shape - 3mm diameter - 3mm height - 290g bond strength.
  • Neodymium type card magnet: parallelepipedal shape - dimensions 6x5x2mm - adhesion strength of 600g.
  • Air gap between the two magnets: about 1mm.

Sur les figures 1 et 2 annexées, de manière non limitative, la carte micro-fluidique 2 est représentée avec deux emplacements distincts pour accueillir deux composants du type décrit ci-dessus sur la même carte.On the figures 1 and 2 attached, in a nonlimiting manner, the microfluidic card 2 is shown with two separate locations for accommodating two components of the type described above on the same card.

On comprend de ce qui précède que la solution de l'invention présente plusieurs avantages, parmi-lesquels :

  • Une facilité d'assemblage du composant sur la carte, notamment grâce à l'utilisation du dispositif magnétique ;
  • Une possibilité de réaliser différentes configurations micro-fluidiques, simplement en remplaçant la puce du composant micro-fluidique 3 ;
  • L'obtention d'une solution fiable, notamment au niveau des connexions micro-fluidiques ;
  • Une réalisation simple, avec un nombre limité de composants ;
It is understood from the above that the solution of the invention has several advantages, among which:
  • Ease of assembly of the component on the card, in particular thanks to the use of the magnetic device;
  • A possibility of making different microfluidic configurations, simply by replacing the micro-fluidic component chip 3;
  • Obtaining a reliable solution, in particular at the level of the microfluidic connections;
  • A simple realization, with a limited number of components;

Claims (6)

  1. Microfluidic device comprising:
    - a microfluidic card (2) which comprises:
    ∘ a first microfluidic structure comprising first microfluidic connection points intended to be passed through by a fluid,
    ∘ a first bearing surface,
    ∘ first means for assembling and centring a microfluidic component (3) on said bearing surface,
    - a microfluidic component (3) which comprises:
    ∘ a second microfluidic structure comprising second microfluidic connection points (311) intended to be passed through by a fluid and microfluidic channels (312) linking its second microfluidic connection points (311) so as to form a network of interconnected microfluidic elements, and intended to be connected to the first microfluidic structure of the microfluidic card (2) to connect said first microfluidic connection points with said second microfluidic connection points,
    ∘ second assembly and centring means arranged to cooperate with said first assembly and centring means of the microfluidic card (2),
    - a seal (4) capable of ensuring a tight connection between the first microfluidic structure and the second microfluidic structure,
    characterized in that:
    - said first assembly and centring means comprise at least one first assembly element based on magnetic effect fixed to said microfluidic card (2) and arranged to cooperate by magnetic effect with a second assembly element based on magnetic effect of said microfluidic component to compress said seal (4) capable of ensuring a tight connection between the first microfluidic structure and the second microfluidic structure,
    - said first assembly and centring means comprise at least one first mechanical member intended to cooperate with a second mechanical member of said second assembly and centring means of said microfluidic component,
    - said first mechanical member comprises at least one cavity (203) produced in the card,
    - said second mechanical member comprises at least one block (303) intended to cooperate by male/female operation with said cavity (203) produced on the microfluidic card (2),
    - said first assembly element based on magnetic effect is housed at the bottom of said cavity (203),
    - said second assembly element based on magnetic effect is housed in said block (303).
  2. Microfluidic device according to Claim 1, characterized in that said first assembly element based on magnetic effect is a permanent magnet (60).
  3. Microfluidic device according to Claim 1, characterized in that said second assembly element based on magnetic effect is a permanent magnet (50).
  4. Microfluidic device according to one of Claims 1 to 3, characterized in that the microfluidic component (3) comprises two mutually separable parts, a first part (30) comprising said means for assembly and centring on said card and a second part (31) comprising said second microfluidic structure.
  5. Device according to one of Claims 1 to 4, characterized in that the seal (4) takes the form of a strip pierced through by several orifices (40), each intended to effect a tight join between a microfluidic connection point of the first microfluidic structure and a microfluidic connection point of the second microfluidic structure.
  6. Device according to Claim 5, characterized in that the seal (4) comprises a bead (41) produced around each of its orifices (40).
EP18161894.3A 2017-03-17 2018-03-15 Microfluidic device Active EP3375523B1 (en)

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EP3190172A2 (en) * 2016-01-07 2017-07-12 Vanderbilt University Interconnections of multiple perfused engineered tissue constructs and microbioreactors, multi-microformulators and applications of the same

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US8337783B2 (en) * 2009-06-23 2012-12-25 The United States of America as represented by the Secretary of Commerce, the National Institute of Standards and Technology Magnetic connectors for microfluidic applications
US9579649B2 (en) * 2010-10-07 2017-02-28 Sandia Corporation Fluid delivery manifolds and microfluidic systems
WO2015175188A1 (en) * 2014-05-14 2015-11-19 General Electric Company Microfluidic flow cell assemblies for imaging and method of use

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