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EP3009189A1 - Objet microfluidique comprenant une chambre de régulation du flux - Google Patents

Objet microfluidique comprenant une chambre de régulation du flux Download PDF

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Publication number
EP3009189A1
EP3009189A1 EP14306645.4A EP14306645A EP3009189A1 EP 3009189 A1 EP3009189 A1 EP 3009189A1 EP 14306645 A EP14306645 A EP 14306645A EP 3009189 A1 EP3009189 A1 EP 3009189A1
Authority
EP
European Patent Office
Prior art keywords
auxiliary
auxiliary channel
chamber
chambers
fluid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP14306645.4A
Other languages
German (de)
English (en)
Inventor
Jean Berthier
Théo CAMBIER
Thibault Honegger
Jérôme Larghero
Manuel Thery
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Universite Grenoble Alpes
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Universite Grenoble Alpes
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA, Universite Grenoble Alpes, Commissariat a lEnergie Atomique et aux Energies Alternatives CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to EP14306645.4A priority Critical patent/EP3009189A1/fr
Publication of EP3009189A1 publication Critical patent/EP3009189A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502746—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means for controlling flow resistance, e.g. flow controllers, baffles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0621—Control of the sequence of chambers filled or emptied
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/087—Multiple sequential chambers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/06—Valves, specific forms thereof
    • B01L2400/0688—Valves, specific forms thereof surface tension valves, capillary stop, capillary break

Definitions

  • the invention relates to a microfluidic device including at least one no-flow chamber.
  • microfluidic device is used to mean a device having at least one dimension that is strictly less than 1 millimeter (mm).
  • the microfluidic device may be for receiving one or more biological cells, especially non-adhesive biological cells.
  • An important aspect of this type of study is to be able to feed the cells with a culture medium that can be renewed easily, but that remains static while the study is taking place.
  • a convective flow through the chamber modifies the culture conditions for the cells. Furthermore, and more critically, the presence of a convective flow can prevent tracking of cells that present little adhesion and that are moved by the flow.
  • Figure 1(a) is a general diagram of that device 100' seen in plan view.
  • Figures 1(b) to 1(d) are perspective views showing the various steps in filling a chamber 20' of that device 100' with a fluid that is to form a culture medium for cells.
  • the device 100' has a main channel 10' and a plurality of chambers 20' situated along and on either side of the main channel 10'.
  • East chamber 20' is for receiving one or more cells for study.
  • Figure 1(b) is an enlargement of a diagram of Figure 1(a) , showing a chamber 20' before being filled with any fluid.
  • the authors make use of chambers made of a material that is porous to air, e.g. polydimethyl siloxane, better known under the acronym PDMS.
  • PDMS polydimethyl siloxane
  • a chamber 20' is thus obtained at that is filled with the fluid that is to form the culture medium for the cells.
  • the chamber 20' is then not subjected to any convective flow, with the fluid passing along the main channel 10' without entering into the chamber 20'.
  • a new fluid is introduced into the device 100' and this fluid enters the chamber 20' by diffusion.
  • That device 100' is rather constraining since it imposes making use of a porous material in order to form the chamber 20'. Without a porous material to form the chamber 20', the chamber 20' would contain air so that the device 100' could not be used.
  • An object of the invention is to mitigate at least one of the above-mentioned drawbacks.
  • the invention provides a microfluidic device characterized in that it comprises at least one row of chambers, said at least one row of chambers comprising :
  • the device according to the invention may also comprise the following features, taken alone or in combination:
  • FIG. 2 shows an embodiment of the invention.
  • the device 100 is a microfluidic device having a main channel 10 with an inlet E for a fluid.
  • the main channel 10 splits into a first auxiliary channel 11 and a second auxiliary channel 12.
  • the device 100 also has a chamber 21 connecting together the two auxiliary channels 11 and 12, each auxiliary channel 11, 12 having, for this purpose, a respective branch connection D 21 , D' 21 leading to the chamber 21.
  • branch connection D 21 , D' 21 , we can hear a connection point or junction between each auxiliary channel 11, 12 and the sub-channel SC21, SC'21 leading to the chamber 21 itself.
  • the sub-channels will not be further referenced in the description which follows, except in figure 8 (SC2i; SC'2i).
  • the device 100 also has a second chamber 22 connecting together the two auxiliary channels 11 and 12, each auxiliary channel 11, 12 having, for this purpose, a respective branch connection D 22 , D' 22 leading to the chamber 22.
  • the branch connections D 21 , D 22 are located on the first auxiliary channel 11.
  • the branch connections D' 21 , D' 22 are located on the second auxiliary channel 12.
  • the chambers 21, 22 are advantageously identical.
  • the auxiliary channels 11, 12 may be arranged such that the distance D between them is constant between the two chambers 21, 22.
  • the first auxiliary channel 11 includes a portion P (drawn in dashed lines) that extends between the main branch connection DP and the branch connection D 21 of the first auxiliary channel 11 going towards the chamber 21, which portion includes a means 20 for causing, during the filling of the device 100, the travel time of the fluid passing via the auxiliary channel 11 between the main branch connection DP and a branch connections D 21 , D 22 of the first auxiliary channel 11 leading to a corresponding chamber 21, 22 to be longer than or equal to the travel time of the fluid passing via the second auxiliary channel 12, a branch connection D' 21 , D' 22 and the corresponding chamber 21, 22 between the main branch connection DP and said branch connection D 21 , D 22 of the first auxiliary channel 11 leading to said corresponding chamber 21, 22.
  • the main branch connection DP coincides with the inlet E 11 of the first auxiliary channel 11 and with the inlet E 12 of the second auxiliary channel 12.
  • the device 100 also has an outlet S for the fluid, which outlet is common to both auxiliary channels 11 and 12.
  • This outlet S is arranged after the last chamber 22 interconnecting the auxiliary 11, 12 channels, namely after the branch connections D 22 and D' 22 .
  • the wording "after” is with reference to the travel direction of the fluid from the inlet towards the common outlet S. This wording will have the same meaning for the remaining of the description with respect to the or each outlet.
  • the means described above form a row of chambers for the device.
  • a fluid e.g. a fluid that is to form a culture medium for cells that are to be received in the chamber 21
  • the fluid reaches the branch connection D 21 via the second auxiliary channel 12, and then the chamber 21 by passing via the branch connection D' 21 , before the same fluid reaches the branch connection D 21 via the first auxiliary channel 11.
  • the chamber 21 can be filled with fluid while expelling the air that was present therein by enabling the air to be exhausted towards the outlet S via the branch connection D 21 .
  • a chamber 21 is thus obtained that does not include a pocket of air.
  • a chamber 21, 22 may have a rectangular or square shape, advantageously with rounded corners, or alternatively a rounded shape. Such a rounded shape avoids any risk that air remains trapped in the chamber 21.
  • air is exhausted from the chamber 21, 22 independently of the nature of the material used for making the chamber 21, 22. It is thus possible to envisage using any type of biocompatible material, and in particular materials that are inexpensive and/or that are not likely to give rise to other problems such as the culture medium evaporating or bacterial contamination through a porous wall.
  • the fluid entering via the inlet E of the device 100 passes via the auxiliary channels 11 and 12 passes towards the outlet S without passing via the chamber 21, because of the pressure balance across the chamber 21, thus making it possible to obtain a chamber 21 in which there is no convective flow.
  • the travel time of the fluid passing via the first auxiliary channel 11 is longer than or equal to the travel time of the fluid passing via the second auxiliary channel 12 between the entry E and the outlet S.
  • the presence of the chamber 22 is also of use to ensure that once the device 100 is full of fluid, this pressure balance is maintained across the chamber 21.
  • the means 20 for causing, during the filling, the travel time of the fluid passing via the first auxiliary channel 11 between the main branch connection DP and a branch connection D 21 , D 22 to be longer than or equal to the travel time of the fluid passing via the second auxiliary channel 12, a branch connection D' 21 , D' 22 and the corresponding chamber 21, 22 between the main branch connection DP and said branch connection D 21 ,D 22 of the first auxiliary channel 11 is advantageously constituted by a means that is passive.
  • this passive means 20 may be formed by a single chamber, a plurality chambers, or indeed one or more zigzag paths.
  • This passive means 20 may be arranged to define a volume that is not less than the volume of the chamber 21 connecting together the two auxiliary channels 11 and 12.
  • the passive means 20 may be a chamber identical to the chamber 21 connecting together the two auxiliary channels 11 and 12.
  • a passive means 20 is advantageous because it is simpler than an active means.
  • the means 20 may be active, for example formed by a valve 20 controlled by a control means 201, for example an electronic control means, adapted for causing, during the filling of the device 100, the travel time of the fluid passing via the auxiliary channel 11 between the main branch connection DP and a branch connections D 21 , D 22 of the first auxiliary channel 11 leading to a corresponding chamber 21, 22 to be longer than or equal to the travel time of the fluid passing via the second auxiliary channel 12, a branch connection D' 21 , D' 22 and the corresponding chamber 21, 22 between the main branch connection DP and said branch connection D 21 , D 22 of the first auxiliary channel 11 leading to said corresponding chamber 21, 22.
  • a control means 201 for example an electronic control means
  • the main channel 10 may have a width lying in the range 10 ⁇ m to 500 ⁇ m.
  • Each auxiliary channel 11 and 12 may have a width lying in the range 10 ⁇ m to 500 ⁇ m.
  • the chamber 21 connecting together the two auxiliary channels 11 and 12 may present sides of at least 100 ⁇ m each.
  • the depths of the channels 10, 11, and 12, and of the chamber 21 should generally be a few tens of micrometers, e.g. 20 ⁇ m.
  • the chamber 22 may have the same dimensions as the dimensions of the first chamber 21.
  • the various channels 10, 11, and 12 may present a section that is circular, rectangular, or square in shape.
  • the device is to be located mainly in a plane (O; X, Y), where the reference frame (0; X, Y, Z) forms a right-handed rectangular frame of reference.
  • the device 100 has a certain thickness (OZ axis), but the phenomena of interest (fluid passing from the inlet towards the outlet; fluid passing through each chamber 21, 22 while it is filling) take place in the two dimensions of the plane (O; X, Y). In this sense, the device constitutes a two-dimensional (2D) device.
  • the device 100 is advantageously to be located in a plane (O; X, Y) that is horizontal.
  • Figures 3(a) , 3(b), and 3(c) show variants of the embodiment of the invention shown in Figure 2 , in which the fluid outlets are different.
  • a respective outlet S 11 , S 12 is provided for each auxiliary channel 11, 12, after the last chamber 22.
  • a common outlet S 11 is provided for both auxiliary channels 11 and 12, which outlet is provided on the first auxiliary channel 11, after the last chamber 22.
  • a common outlet S 12 is provided for both auxiliary channels 11 and 12, which outlet is provided on the second auxiliary channel 12, after the last chamber 22.
  • the or each outlet is more precisely located after a branch connection D 22 or D' 22 . All of the other means in these variant embodiments are identical to those of the embodiment shown in Figure 2 .
  • Figure 4 shows another embodiment of the invention.
  • the chambers 21, 22, ..., 2n are advantageously identical.
  • the chambers 21, 22, ..., 2n are advantageously arranged at regular intervals along said auxiliary channels 11 and 12. In this situation, this means that the distance d between two successive chambers 21, 22, ..., 2n and more precisely between two successive branch connections to an auxiliary channel 11, 12 leading to one of the chambers in the plurality of chambers 21, 22, ..., 2n is constant. This is as shown in Figure 4 .
  • auxiliary channels 11, 12 are advantageously arranged at a constant distance D between at least two successive chambers of said plurality of chambers 21, 22, ..., 2n interconnecting the two auxiliary channels 11, 12.
  • the auxiliary channels 11, 12 are arranged at a constant distance D between the first chamber 21 and the last chamber 2n interconnecting the two auxiliary channels 11, 12.
  • the device 100 may comprise at least five chambers 21, 22, ..., 2n interconnecting the two auxiliary channels 11, 12, or at least eight chambers interconnecting the two auxiliary channels 11, 12, or at least ten chambers interconnecting the two auxiliary channels 11, 12.
  • the device 100 will comprise at least twenty of these chambers or even at least thirthy of these chambers. Numerous chambers allow testing numerous cells in the same device.
  • An advantage of providing numerous chambers 21, 22, ..., 2n is to be able to test numerous cells in the various chambers. Furthermore, and as explained below, this also makes it possible to increase the filling rates of the device 100, while keeping certain chambers without any convective flow.
  • FIG. 6 shows another embodiment of the invention.
  • the rows R1 and R3 are arranged in parallel with the row R2.
  • the rows R1 and R3 are advantageously identical to the row R2.
  • main channel 10 In order to feed these rows, it is then appropriate to provide the main channel 10 with a main row branch connection DPR suitable for feeding each of the rows R1, R2, and R3.
  • This branch connection DPR is upstream from the main branch connection DP, "upstream" being relative to the flow direction of the fluid in the device 100.
  • This branch connection DPR serves to feed the main channel 102, 103 of each additional row R1, R3.
  • the main branch connection DP coincides with the inlet E 11 of the first auxiliary channel 11 and with the inlet E 12 of the second auxiliary channel 12.
  • each has a single respective outlet S 11,R1 , S 11,R2 , or S 11,R3 arranged as shown in the diagram of Figure 4 .
  • a common outlet may be provided connected to one of the auxiliary channels of each row, which may be an auxiliary channel of the same type as the channel 11 ( Figure 4 ), or which may be an auxiliary channel of the same type as the channel 12 ( Figure 5 ).
  • Figure 6 merely shows one example, and more generally it is possible to provide N rows, with N > 1.
  • FIG 7 there can be seen a device 100 in accordance with the invention that has been used for performing experimental tests.
  • the means 20 for providing a time offset is a chamber identical to the other chambers.
  • the chamber numbered 1 corresponds to a chamber 20 (cf. Figures 2 to 6 ) forming the passive means for imparting a time offset while filling of the experimental device with the fluid.
  • each row R1 to R24 provides an outlet arranged in accordance with Figures 4 and 6 .
  • the first auxiliary channel 11 presents a section that is rectangular, of width (axis OY) equal to 75 ⁇ m.
  • the chamber 2i presents a shape that is substantially square with rounded corners, and each side presents a length of 300 ⁇ m (axis OY or axis OX).
  • the second auxiliary channel 12 presents a section that is rectangular, of width (axis OY) equal to 100 ⁇ m.
  • the passage connecting the chamber 2i to each of the auxiliary channels 11, 12 presents a width of 50 ⁇ m. For all of these means, the depth (axis OZ) is 20 ⁇ m.
  • the channel presents a width of 500 ⁇ m, still with a depth of 20 ⁇ m.
  • the experimental device was made of PDMS.
  • a layer of positive photosensitive resist (AZ9260; AZ Electronic Materials) was spin-coated onto a silicon substrate at 750 revolutions per minute (rpm) for 60 seconds (s). That produced a resist layer having a thickness of 20 ⁇ m.
  • the resist once on its substrate, was pre-baked for 1 minute (min) at 60°C and then for 4 min at 110°C.
  • UV ultraviolet
  • the layer of photosensitive resist was then dried by air-blow.
  • PDMS (kit sylgard, Dow Corning) was mixed with a curing agent, degassed, poured into a mold, and cured for 1 hour (h) at 100°C on a hot plate.
  • the layer of PDMS was then removed.
  • the PDMS having the shape desired for making the experimental device, and a standard glass slide (borosilicate glass) were oxidized under plasma for 10 s at 100 W (Femto, Diener Electronics) and put into contact to ensure bonding and prevent any leakage of fluid in use. Prior to bonding, orifices were formed in the PDMS for subsequent connection with the fluid feed.
  • the connections were made for the fluid feeds.
  • the device as obtained at this stage was connected to a pump via capillaries, the capillaries being inserted in the orifices in the PDMS.
  • the other ends of the capillaries were connected to a reservoir for containing the fluid for feeding to the experimental device.
  • the fluid used was water.
  • Figure 9 is an experimental display showing how the experimental device becomes filled as a function of time.
  • the chamber number for the row in question is plotted along the abscissa axis ("position"), and the fluid flow speed V C through the chamber in question is plotted up the ordinate axis (it should be recalled that the chamber numbered 1 is the chamber that is used for imparting the time offset; it corresponds to the chamber 20 in Figures 4 to 6 ).
  • the last chambers i.e. the chambers numbered 33 to 36, are likewise subjected to the passage of fluid.
  • the inventors believe that this is associated with the fact that the second auxiliary channel does not continue beyond the last chamber 2n, specifically on the second auxiliary channel 12, which implies a special limit condition.
  • an interesting aspect lies in the fact that, for an inlet fluid flow speed of 150 ⁇ m/s, only the chambers numbered 2 and 3 are subjected to passing a (convective) flow, whereas for the flow speed of 1500 ⁇ m/s, it is the chambers numbered 2, 3, 4, and 5 that are subjected to a flow. Furthermore, when considering a given chamber, e.g.
  • Reducing the fluid flow speed at the inlet thus makes it possible to limit the number of chambers that are subjected to a convective flow of fluid.
  • n 2 in order to study numerous cells at the same time and in order to be able to change the culture medium quickly, typically in a few minutes.
  • n 2 in order to study numerous cells at the same time and in order to be able to change the culture medium quickly, typically in a few minutes.
  • the fluid contained particles identifiable with the method used for producing the display (the particles appear white).
  • Figure 12 is an enlargement of Figure 11 showing chambers without flow.
  • Another interesting aspect of the invention relates to renewing the fluid.
  • the device 100 may be made of other types of materials than PDMS, as a porous medium is not necessary for the chambers 20, 21, ..., 2n.
  • the device 100 may be made of plastics such as polystyrene (PS), polycarbonate (PC), polymethylmethacrylate (PMMA) or polypropylene (PP).
  • PS polystyrene
  • PC polycarbonate
  • PMMA polymethylmethacrylate
  • PP polypropylene
  • the companies Greiner or Ibidi provides such plastics for microfluidic applications.
  • the device 100 may be made of Thermoset Polyester (TPE), Polyurethane Methacrylate (PUMA) or NorlandAdhesive 81 (NOA81).
  • TPE Thermoset Polyester
  • PUMA Polyurethane Methacrylate
  • NOA81 NorlandAdhesive 81
  • the first auxiliary channel 11 includes a portion P that extends between the inlet E 11 of this first auxiliary channel 11 and the branch connection D 21 of the first auxiliary channel 11 going towards a first chamber 21, which portion includes a means 20 for causing, during the filing of the device, the travel time of the fluid passing via the first auxiliary channel 11 between the inlet E 11 of the first auxiliary channel 11 and a branch connection D 21 , D 22 of the first auxiliary channel 11 to be longer than or equal to the travel time of the fluid passing via the second auxiliary channel 12, a branch connection D' 21 , D' 22 of the second auxiliary channel 12 and the corresponding chamber 21, 22 between the inlet E 12 of the second auxiliary channel 12 and said branch connection D 21 , D 22 of the first auxiliary channel 11.
  • the invention also proposes a system 1000 comprising:
  • FIG 14(a) This is illustrated in figure 14(a) , with, in this example, a device 100 having one entry E and one outlet S.
  • the means 101 may be a reservoir.
  • the reservoir 101 will contain the fluid.
  • this reservoir will be a culture medium.
  • a fluidic connection 105 may be envisaged between the means 101 and the entry E of the device 100 according to the invention.
  • the system 1000 will comprise a means 102 for receiving the fluid exhausted from said device 100, as illustrated in figure 14(a) .
  • a fluidic connection 106 may be envisaged between the outlet S of the device 100 according to the invention and said means 102.
  • the means 102 may be reservoir, as illustrated in figure 14(a) .
  • system 1000 may further comprise a fluid connection 103 linking said means 102 to said means 101 and comprising a pump 104 leading the fluid from said means 103 to said means 101.

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP14306645.4A 2014-10-16 2014-10-16 Objet microfluidique comprenant une chambre de régulation du flux Withdrawn EP3009189A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP14306645.4A EP3009189A1 (fr) 2014-10-16 2014-10-16 Objet microfluidique comprenant une chambre de régulation du flux

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP14306645.4A EP3009189A1 (fr) 2014-10-16 2014-10-16 Objet microfluidique comprenant une chambre de régulation du flux

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EP3009189A1 true EP3009189A1 (fr) 2016-04-20

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2213364A1 (fr) * 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Motifs de guide de phase pour la manipulation de liquides
US20120071358A1 (en) * 2008-12-04 2012-03-22 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
US20120196280A1 (en) * 2009-07-17 2012-08-02 Norchip A/S Microfabricated device for metering an analyte
WO2014038943A1 (fr) * 2012-09-10 2014-03-13 Universiteit Leiden Perfectionnements apportés à des barrières de rétention de pression capillaire

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20120071358A1 (en) * 2008-12-04 2012-03-22 Xiaochuan Zhou Fluidic devices and methods for multiplex chemical and biochemical reactions
EP2213364A1 (fr) * 2009-01-30 2010-08-04 Albert-Ludwigs-Universität Freiburg Motifs de guide de phase pour la manipulation de liquides
US20120196280A1 (en) * 2009-07-17 2012-08-02 Norchip A/S Microfabricated device for metering an analyte
WO2014038943A1 (fr) * 2012-09-10 2014-03-13 Universiteit Leiden Perfectionnements apportés à des barrières de rétention de pression capillaire

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHUNXIONG LUO: "High-throughput microfluidic system for monitoring diffusion-based monolayer yeast cell structure over long time periods", BIOMED MICRODEVICES, vol. 11, 2009, pages 981 - 986, XP019746507, DOI: doi:10.1007/s10544-009-9315-7

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