[go: up one dir, main page]

EP2872652A4 - Devices and methods for nucleic acid preparation and analysis - Google Patents

Devices and methods for nucleic acid preparation and analysis

Info

Publication number
EP2872652A4
EP2872652A4 EP13778675.2A EP13778675A EP2872652A4 EP 2872652 A4 EP2872652 A4 EP 2872652A4 EP 13778675 A EP13778675 A EP 13778675A EP 2872652 A4 EP2872652 A4 EP 2872652A4
Authority
EP
European Patent Office
Prior art keywords
analysis
methods
devices
nucleic acid
acid preparation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP13778675.2A
Other languages
German (de)
French (fr)
Other versions
EP2872652A1 (en
Inventor
John L Harris
Adrian Mark Thomas West
Broeck Dirk Peter Ten
Ekaterina Protozanova
Robert H Meltzer
Joshua W Griffis
Rudolf Gilmanshin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
PathoGenetix Inc
Original Assignee
PathoGenetix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by PathoGenetix Inc filed Critical PathoGenetix Inc
Publication of EP2872652A1 publication Critical patent/EP2872652A1/en
Publication of EP2872652A4 publication Critical patent/EP2872652A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0636—Focussing flows, e.g. to laminate flows
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0663—Stretching or orienting elongated molecules or particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B01L2400/049—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics vacuum

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
EP13778675.2A 2012-04-18 2013-04-18 Devices and methods for nucleic acid preparation and analysis Withdrawn EP2872652A4 (en)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US201261635263P 2012-04-18 2012-04-18
US201261625745P 2012-04-18 2012-04-18
US201261625743P 2012-04-18 2012-04-18
US201261635221P 2012-04-18 2012-04-18
US201361783601P 2013-03-14 2013-03-14
US201361784399P 2013-03-14 2013-03-14
US201361784061P 2013-03-14 2013-03-14
US201361790721P 2013-03-15 2013-03-15
PCT/US2013/037140 WO2013158860A1 (en) 2012-04-18 2013-04-18 Devices and methods for nucleic acid preparation and analysis

Publications (2)

Publication Number Publication Date
EP2872652A1 EP2872652A1 (en) 2015-05-20
EP2872652A4 true EP2872652A4 (en) 2016-03-09

Family

ID=49384067

Family Applications (1)

Application Number Title Priority Date Filing Date
EP13778675.2A Withdrawn EP2872652A4 (en) 2012-04-18 2013-04-18 Devices and methods for nucleic acid preparation and analysis

Country Status (2)

Country Link
EP (1) EP2872652A4 (en)
WO (1) WO2013158860A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11597646B2 (en) 2016-07-26 2023-03-07 Hewlett-Packard Development Company, L.P. Microfluidic device with manifold
WO2018106897A1 (en) * 2016-12-07 2018-06-14 Niagara Biosciences, Inc. Devices and methods for nucleic acid identification
CN109622072B (en) * 2019-02-28 2023-08-22 枣庄学院 A storage and recovery device for laboratory waste liquid
CN111718836B (en) * 2020-06-16 2022-08-26 东南大学 Microfluidic chip for rare cell acquisition and single cell packaging
CN114682310B (en) * 2020-12-31 2023-12-05 深圳市真迈生物科技有限公司 Liquid path system, sequencing system and method

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000009757A1 (en) * 1998-08-13 2000-02-24 U.S. Genomics, Inc. Optically characterizing polymers
WO2001013088A1 (en) * 1999-08-13 2001-02-22 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
WO2004091795A2 (en) * 2003-04-10 2004-10-28 U.S. Genomics, Inc. Manipulation of polymers in a microchannel

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6696022B1 (en) * 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US20050142565A1 (en) * 2003-12-30 2005-06-30 Agency For Science, Technology And Research Nucleic acid purification chip
CN101166834B (en) * 2005-04-30 2011-09-14 三星电子股份有限公司 Bio-disc, bio-driver apparatus, and assay method using the same
WO2006122311A2 (en) * 2005-05-11 2006-11-16 The Trustees Of The University Of Pennsylvania Microfluidic chip
US8114636B2 (en) * 2006-02-10 2012-02-14 Life Technologies Corporation Labeling and detection of nucleic acids
WO2008052138A2 (en) * 2006-10-25 2008-05-02 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated dna analysis system using same

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2000009757A1 (en) * 1998-08-13 2000-02-24 U.S. Genomics, Inc. Optically characterizing polymers
WO2001013088A1 (en) * 1999-08-13 2001-02-22 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
WO2004091795A2 (en) * 2003-04-10 2004-10-28 U.S. Genomics, Inc. Manipulation of polymers in a microchannel

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BAKAJIN O B ET AL: "ELECTROHYDRODYNAMIC STRETCHING OF DNA IN CONFINED ENVIRONMENTS", PHYSICAL REVIEW LETTERS, AMERICAN PHYSICAL SOCIETY, US, vol. 80, no. 12, 23 March 1998 (1998-03-23), pages 2737 - 2740, XP000980688, ISSN: 0031-9007, DOI: 10.1103/PHYSREVLETT.80.2737 *
CHAN E Y ET AL: "DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags", GENOME RESEARCH, COLD SPRING HARBOR LABORATORY PRESS, vol. 14, no. 6, 1 June 2004 (2004-06-01), pages 1137 - 1146, XP002346048, ISSN: 1088-9051, DOI: 10.1101/GR.1635204 *
See also references of WO2013158860A1 *

Also Published As

Publication number Publication date
EP2872652A1 (en) 2015-05-20
WO2013158860A1 (en) 2013-10-24

Similar Documents

Publication Publication Date Title
IL293796A (en) Methods for nucleic acid assembly and high throughput sequencing
IL257714B (en) Systems and methods for multiple analyte analysis
IL235128A0 (en) Nucleic acid sample preparation
EP2702175A4 (en) Methods and compositions for nucleic acid analysis
HK1213944A1 (en) Methods for targeted genomic analysis
SG11201405785WA (en) Systems and methods for biological analysis
HK1216653A1 (en) Multiplex nucleic acid detection methods
ZA201407220B (en) Methods for preparing samples for nucleic acid amplification
HK1207904A1 (en) Improved analyte measurement technique and system
HK1217117A1 (en) Formulations and methods for stabilizing pcr reagents pcr
EP2878953A4 (en) Analysis device
EP2909346A4 (en) Method and system for sample preparation
GB201119903D0 (en) Nucleic acid production and sequence analysis
EP2893034A4 (en) Method for multiplex nucleic acid analysis
PL2854981T3 (en) Methods of nucleic acid fractionation and detection
EP2740804A4 (en) Nucleic acid analysis method
PL2633914T5 (en) Pipetting device and method for its production
EP2909343A4 (en) Methods and apparatus to sequence a nucleic acid
EP2872652A4 (en) Devices and methods for nucleic acid preparation and analysis
EP2899655A4 (en) Analysis device
EP2844767A4 (en) Nucleic acid sequencing systems and methods
EP2899656A4 (en) Analysis method and analysis device
EP2878673A4 (en) Preparation method and preparation device for sample for nucleic acid amplification reaction
EP2843047A4 (en) Nucleic acid detection method
IL234932B (en) Method and structure for chemical analysis

Legal Events

Date Code Title Description
PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

Free format text: ORIGINAL CODE: 0009012

17P Request for examination filed

Effective date: 20150313

AK Designated contracting states

Kind code of ref document: A1

Designated state(s): AL AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO RS SE SI SK SM TR

AX Request for extension of the european patent

Extension state: BA ME

RIN1 Information on inventor provided before grant (corrected)

Inventor name: GRIFFIS, JOSHUA, W.

Inventor name: PROTOZANOVA, EKATERINA

Inventor name: HARRIS, JOHN, L.

Inventor name: WEST, ADRIAN MARK, THOMAS

Inventor name: TEN BROECK, DIRK, PETER

Inventor name: GILMANSHIN, RUDOLF

Inventor name: MELTZER, ROBERT H.

DAX Request for extension of the european patent (deleted)
RA4 Supplementary search report drawn up and despatched (corrected)

Effective date: 20160204

RIC1 Information provided on ipc code assigned before grant

Ipc: C12N 15/10 20060101ALI20160129BHEP

Ipc: C12Q 1/68 20060101AFI20160129BHEP

Ipc: C12N 15/11 20060101ALI20160129BHEP

Ipc: G01N 35/08 20060101ALI20160129BHEP

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20160906