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EP2794872A4 - Polypeptides having beta-glucosidase activity and polynucleotides encoding same - Google Patents

Polypeptides having beta-glucosidase activity and polynucleotides encoding same

Info

Publication number
EP2794872A4
EP2794872A4 EP12860010.3A EP12860010A EP2794872A4 EP 2794872 A4 EP2794872 A4 EP 2794872A4 EP 12860010 A EP12860010 A EP 12860010A EP 2794872 A4 EP2794872 A4 EP 2794872A4
Authority
EP
European Patent Office
Prior art keywords
polypeptides
beta
polynucleotides encoding
glucosidase activity
encoding same
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP12860010.3A
Other languages
German (de)
French (fr)
Other versions
EP2794872A1 (en
Inventor
Junxin Duan
Yu Zhang
Lan Tang
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Novozymes Inc
Original Assignee
Novozymes Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes Inc filed Critical Novozymes Inc
Publication of EP2794872A1 publication Critical patent/EP2794872A1/en
Publication of EP2794872A4 publication Critical patent/EP2794872A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1137—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C12N15/8246—Non-starch polysaccharides, e.g. cellulose, fructans, levans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
EP12860010.3A 2011-12-19 2012-12-19 Polypeptides having beta-glucosidase activity and polynucleotides encoding same Withdrawn EP2794872A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN2011084228 2011-12-19
PCT/CN2012/086938 WO2013091544A1 (en) 2011-12-19 2012-12-19 Polypeptides having beta-glucosidase activity and polynucleotides encoding same

Publications (2)

Publication Number Publication Date
EP2794872A1 EP2794872A1 (en) 2014-10-29
EP2794872A4 true EP2794872A4 (en) 2015-12-09

Family

ID=48667711

Family Applications (1)

Application Number Title Priority Date Filing Date
EP12860010.3A Withdrawn EP2794872A4 (en) 2011-12-19 2012-12-19 Polypeptides having beta-glucosidase activity and polynucleotides encoding same

Country Status (4)

Country Link
US (1) US9771568B2 (en)
EP (1) EP2794872A4 (en)
BR (1) BR112014014760A2 (en)
WO (1) WO2013091544A1 (en)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014060379A1 (en) * 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Cell wall deconstruction enzymes of myceliophthora fergusii (corynascus thermophilus) and uses thereof
EP2826858B1 (en) * 2013-07-09 2017-05-31 Honda Motor Co., Ltd. Beta-glucosidase
US9228181B2 (en) 2013-07-09 2016-01-05 Honda Motor Co., Ltd. β-glucosidase
EP2824179B1 (en) * 2013-07-09 2017-11-29 Honda Motor Co., Ltd. Beta-glucosidase
EP2824178B1 (en) 2013-07-09 2017-12-06 Honda Motor Co., Ltd. ß-glucosidase
EP4386134A3 (en) 2015-04-10 2024-08-28 Comet Biorefining Inc. Methods and compositions for the treatment of cellulosic biomass and products produced thereby
CN108883400B (en) 2016-02-19 2021-09-17 洲际大品牌有限责任公司 Method for forming a multi-value stream from a biomass source
CN109385411B (en) * 2017-08-04 2021-10-26 东莞泛亚太生物科技有限公司 Beta-mannosidase and application thereof
CN107365780A (en) * 2017-08-10 2017-11-21 广东省生物资源应用研究所 A kind of clone of eastern subterranean termite salivary gland β glycosidase genes and prokaryotic expression method
US10633461B2 (en) 2018-05-10 2020-04-28 Comet Biorefining Inc. Compositions comprising glucose and hemicellulose and their use
US20220307036A1 (en) 2019-08-06 2022-09-29 Novozymes A/S Fusion proteins for improved enzyme expression

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066457A2 (en) * 2009-11-25 2011-06-03 Codexis, Inc. Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass
WO2014060379A1 (en) * 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Cell wall deconstruction enzymes of myceliophthora fergusii (corynascus thermophilus) and uses thereof
WO2014059541A1 (en) * 2012-10-16 2014-04-24 Concordia University Novel cell wall deconstruction enzymes of thermoascus aurantiacus, myceliophthora fergusii (corynascus thermophilus), and pseudocercosporella herpotrichoides, and uses thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2009073723A1 (en) * 2007-12-06 2009-06-11 Novozymes A/S Polypeptides having beta-glucosidase activity and polynucleotides encoding same
CN102712916B (en) 2009-09-18 2015-11-25 诺维信股份有限公司 There are the polypeptide of beta-glucosidase activity and the polynucleotide of this polypeptide of coding

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011066457A2 (en) * 2009-11-25 2011-06-03 Codexis, Inc. Recombinant beta-glucosidase variants for production of soluble sugars from cellulosic biomass
WO2014060379A1 (en) * 2012-10-16 2014-04-24 Dsm Ip Assets B.V. Cell wall deconstruction enzymes of myceliophthora fergusii (corynascus thermophilus) and uses thereof
WO2014059541A1 (en) * 2012-10-16 2014-04-24 Concordia University Novel cell wall deconstruction enzymes of thermoascus aurantiacus, myceliophthora fergusii (corynascus thermophilus), and pseudocercosporella herpotrichoides, and uses thereof

Non-Patent Citations (7)

* Cited by examiner, † Cited by third party
Title
COLLINS ET AL: "Molecular cloning and expression analysis of two distinct beta-glucosidase genes, bg1 and aven1, with very different biological roles from the thermophilic, saprophytic fungus Talaromyces emersonii", MYCOLOGICAL RESEARCH, ELSEVIER, GB, vol. 111, no. 7, 6 August 2007 (2007-08-06), pages 840 - 849, XP022188075, ISSN: 0953-7562, DOI: 10.1016/J.MYCRES.2007.05.007 *
KRISTIAN B R M KROGH ET AL: "Characterization and kinetic analysis of a thermostable GH3 beta-glucosidase from Penicillium brasilianum", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, SPRINGER, BERLIN, DE, vol. 86, no. 1, 16 September 2009 (2009-09-16), pages 143 - 154, XP019799835, ISSN: 1432-0614 *
LEONTINA GURGU ET AL: "Fermentation of cellobiose to ethanol by industrial strains carrying the beta-glucosidase gene (BGL1) from Saccharomyces fibuligera", BIORESOURCE TECHNOLOGY, ELSEVIER BV, GB, vol. 102, no. 8, 19 January 2011 (2011-01-19), pages 5229 - 5236, XP028162740, ISSN: 0960-8524, [retrieved on 20110125], DOI: 10.1016/J.BIORTECH.2011.01.062 *
RAJ KUMAR ET AL: "Bioconversion of lignocellulosic biomass: biochemical and molecular perspectives", JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY ; OFFICIAL JOURNAL OF THE SOCIETY FOR INDUSTRIAL MICROBIOLOGY, SPRINGER, BERLIN, DE, vol. 35, no. 5, 13 March 2008 (2008-03-13), pages 377 - 391, XP019596328, ISSN: 1476-5535 *
ROSGAARD LISA ET AL: "Efficiency of new fungal cellulase systems in boosting enzymatic degradation of barley straw lignocellulose", BIOTECHNOLOGY PROGRESS, AMERICAN INSTITUTE OF CHEMICAL ENGINEERS, US, vol. 22, no. 2, 1 March 2006 (2006-03-01), pages 493 - 498, XP002565690, ISSN: 8756-7938, [retrieved on 20060324], DOI: 10.1021/BP050361O *
SAUMITA BANERJEE ET AL: "Commercializing lignocellulosic bioethanol: technology bottlenecks and possible remedies", BIOFUELS, BIOPRODUCTS AND BIOREFINING, vol. 4, no. 1, 1 January 2010 (2010-01-01), pages 77 - 93, XP055200685, ISSN: 1932-104X, DOI: 10.1002/bbb.188 *
See also references of WO2013091544A1 *

Also Published As

Publication number Publication date
EP2794872A1 (en) 2014-10-29
US20140331364A1 (en) 2014-11-06
WO2013091544A1 (en) 2013-06-27
US9771568B2 (en) 2017-09-26
BR112014014760A2 (en) 2017-06-13

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