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EP2635840A4 - EMULSION FORMATION SYSTEM - Google Patents

EMULSION FORMATION SYSTEM

Info

Publication number
EP2635840A4
EP2635840A4 EP20110838713 EP11838713A EP2635840A4 EP 2635840 A4 EP2635840 A4 EP 2635840A4 EP 20110838713 EP20110838713 EP 20110838713 EP 11838713 A EP11838713 A EP 11838713A EP 2635840 A4 EP2635840 A4 EP 2635840A4
Authority
EP
European Patent Office
Prior art keywords
formation system
emulsion formation
emulsion
formation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP20110838713
Other languages
German (de)
French (fr)
Other versions
EP2635840B1 (en
EP2635840A2 (en
Inventor
Amy L Hiddessen
Donald A Masquelier
Kevin D Ness
Benjamin J Hindson
Anthony J Makarewicz Jr
Erin R Steenblock
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bio Rad Laboratories Inc
Original Assignee
Bio Rad Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=46025074&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EP2635840(A4) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Bio Rad Laboratories Inc filed Critical Bio Rad Laboratories Inc
Priority to EP19186085.7A priority Critical patent/EP3574990B1/en
Priority to EP16190414.9A priority patent/EP3132844B1/en
Priority to EP22153574.3A priority patent/EP4016086A1/en
Publication of EP2635840A2 publication Critical patent/EP2635840A2/en
Publication of EP2635840A4 publication Critical patent/EP2635840A4/en
Application granted granted Critical
Publication of EP2635840B1 publication Critical patent/EP2635840B1/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B01F23/4105—Methods of emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B01F23/414—Emulsifying characterised by the internal structure of the emulsion
    • B01F23/4145—Emulsions of oils, e.g. fuel, and water
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F25/00—Flow mixers; Mixers for falling materials, e.g. solid particles
    • B01F25/14—Mixing drops, droplets or bodies of liquid which flow together or contact each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/21—Measuring
    • B01F35/211—Measuring of the operational parameters
    • B01F35/2113—Pressure
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F35/00—Accessories for mixers; Auxiliary operations or auxiliary devices; Parts or details of general application
    • B01F35/20—Measuring; Control or regulation
    • B01F35/22—Control or regulation
    • B01F35/221—Control or regulation of operational parameters, e.g. level of material in the mixer, temperature or pressure
    • B01F35/2213—Pressure
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/38—Diluting, dispersing or mixing samples
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/2202—Mixing compositions or mixers in the medical or veterinary field
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
    • B01F2101/44—Mixing of ingredients for microbiology, enzymology, in vitro culture or genetic manipulation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
    • B01F23/40—Mixing liquids with liquids; Emulsifying
    • B01F23/41—Emulsifying
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/30—Micromixers
    • B01F33/301—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions
    • B01F33/3011—Micromixers using specific means for arranging the streams to be mixed, e.g. channel geometries or dispositions using a sheathing stream of a fluid surrounding a central stream of a different fluid, e.g. for reducing the cross-section of the central stream or to produce droplets from the central stream
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
    • B01F33/00—Other mixers; Mixing plants; Combinations of mixers
    • B01F33/80—Mixing plants; Combinations of mixers
    • B01F33/81—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles
    • B01F33/813—Combinations of similar mixers, e.g. with rotary stirring devices in two or more receptacles mixing simultaneously in two or more mixing receptacles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0605—Metering of fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
    • B01L2400/0487—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure fluid pressure, pneumatics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads, for physically stretching molecules
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1046—Levitated, suspended drops
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/0318—Processes
    • Y10T137/0396—Involving pressure control
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10T—TECHNICAL SUBJECTS COVERED BY FORMER US CLASSIFICATION
    • Y10T137/00—Fluid handling
    • Y10T137/8593—Systems

Landscapes

  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Health & Medical Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Colloid Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Sampling And Sample Adjustment (AREA)
  • Micromachines (AREA)
  • Processes Of Treating Macromolecular Substances (AREA)
EP11838713.3A 2010-11-01 2011-11-01 System for forming emulsions Active EP2635840B1 (en)

Priority Applications (3)

Application Number Priority Date Filing Date Title
EP19186085.7A EP3574990B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP16190414.9A EP3132844B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP22153574.3A EP4016086A1 (en) 2010-11-01 2011-11-01 System for forming emulsions

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US40910610P 2010-11-01 2010-11-01
US40947310P 2010-11-02 2010-11-02
US41076910P 2010-11-05 2010-11-05
PCT/US2011/058857 WO2012061444A2 (en) 2010-11-01 2011-11-01 System for forming emulsions

Related Child Applications (4)

Application Number Title Priority Date Filing Date
EP22153574.3A Division EP4016086A1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP16190414.9A Division-Into EP3132844B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP16190414.9A Division EP3132844B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP19186085.7A Division EP3574990B1 (en) 2010-11-01 2011-11-01 System for forming emulsions

Publications (3)

Publication Number Publication Date
EP2635840A2 EP2635840A2 (en) 2013-09-11
EP2635840A4 true EP2635840A4 (en) 2014-10-08
EP2635840B1 EP2635840B1 (en) 2017-01-04

Family

ID=46025074

Family Applications (4)

Application Number Title Priority Date Filing Date
EP19186085.7A Active EP3574990B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP16190414.9A Active EP3132844B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP22153574.3A Pending EP4016086A1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP11838713.3A Active EP2635840B1 (en) 2010-11-01 2011-11-01 System for forming emulsions

Family Applications Before (3)

Application Number Title Priority Date Filing Date
EP19186085.7A Active EP3574990B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP16190414.9A Active EP3132844B1 (en) 2010-11-01 2011-11-01 System for forming emulsions
EP22153574.3A Pending EP4016086A1 (en) 2010-11-01 2011-11-01 System for forming emulsions

Country Status (9)

Country Link
US (1) US9089844B2 (en)
EP (4) EP3574990B1 (en)
JP (5) JP5922139B2 (en)
CN (2) CN103429331B (en)
AU (1) AU2011323422B2 (en)
CA (4) CA3215088A1 (en)
DE (1) DE202011110979U1 (en)
SG (1) SG190074A1 (en)
WO (1) WO2012061444A2 (en)

Families Citing this family (148)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8298833B2 (en) 2006-02-07 2012-10-30 Stokes Bio Limited Liquid bridge and system
US20140024023A1 (en) * 2012-07-23 2014-01-23 Bio- Rad Laboratories, Inc Droplet generation system with features for sample positioning
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
AU2009297108B2 (en) 2008-09-23 2015-02-12 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9492797B2 (en) 2008-09-23 2016-11-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US12162008B2 (en) 2008-09-23 2024-12-10 Bio-Rad Laboratories, Inc. Partition-based method of analysis
US11130128B2 (en) 2008-09-23 2021-09-28 Bio-Rad Laboratories, Inc. Detection method for a target nucleic acid
US9417190B2 (en) 2008-09-23 2016-08-16 Bio-Rad Laboratories, Inc. Calibrations and controls for droplet-based assays
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US12090480B2 (en) 2008-09-23 2024-09-17 Bio-Rad Laboratories, Inc. Partition-based method of analysis
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US9222128B2 (en) 2011-03-18 2015-12-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US10512910B2 (en) 2008-09-23 2019-12-24 Bio-Rad Laboratories, Inc. Droplet-based analysis method
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
CA3106547C (en) 2009-09-02 2022-07-05 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
EP2550351A4 (en) 2010-03-25 2014-07-09 Quantalife Inc Detection system for droplet-based assays
CA2767114A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Droplet transport system for detection
US12097495B2 (en) 2011-02-18 2024-09-24 Bio-Rad Laboratories, Inc. Methods and compositions for detecting genetic material
WO2012149042A2 (en) 2011-04-25 2012-11-01 Bio-Rad Laboratories, Inc. Methods and compositions for nucleic acid analysis
EP2737089B1 (en) 2011-07-29 2017-09-06 Bio-rad Laboratories, Inc. Library characterization by digital assay
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
WO2013019491A1 (en) 2011-08-01 2013-02-07 Denovo Sciences Cell capture system and method of use
WO2013155531A2 (en) 2012-04-13 2013-10-17 Bio-Rad Laboratories, Inc. Sample holder with a well having a wicking promoter
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2014028537A1 (en) 2012-08-14 2014-02-20 10X Technologies, Inc. Microcapsule compositions and methods
WO2014085802A1 (en) * 2012-11-30 2014-06-05 The Broad Institute, Inc. High-throughput dynamic reagent delivery system
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP2951320B1 (en) 2013-02-01 2018-12-26 Bio-Rad Laboratories, Inc. Multiplexed digital assay with specific and generic reporters
US9644204B2 (en) 2013-02-08 2017-05-09 10X Genomics, Inc. Partitioning and processing of analytes and other species
US9707562B2 (en) * 2013-03-13 2017-07-18 Denovo Sciences, Inc. System for capturing and analyzing cells
JP6449266B2 (en) * 2013-06-21 2019-01-09 バイオ−ラッド・ラボラトリーズ・インコーポレーテッド Microfluidic system with fluid pickup
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
WO2015157567A1 (en) 2014-04-10 2015-10-15 10X Genomics, Inc. Fluidic devices, systems, and methods for encapsulating and partitioning reagents, and applications of same
US9909167B2 (en) 2014-06-23 2018-03-06 The Board Of Trustees Of The Leland Stanford Junior University On-slide staining by primer extension
MX2016016902A (en) 2014-06-26 2017-03-27 10X Genomics Inc Methods of analyzing nucleic acids from individual cells or cell populations.
JP2017522866A (en) 2014-06-26 2017-08-17 １０エックス ジェノミクス， インコーポレイテッド Nucleic acid sequence analysis
US12312640B2 (en) 2014-06-26 2025-05-27 10X Genomics, Inc. Analysis of nucleic acid sequences
GB201415789D0 (en) * 2014-09-05 2014-10-22 Touchlight Genetics Ltd Synthesis of DNA
WO2016040476A1 (en) 2014-09-09 2016-03-17 The Broad Institute, Inc. A droplet-based method and apparatus for composite single-cell nucleic acid analysis
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
AU2016207023B2 (en) 2015-01-12 2019-12-05 10X Genomics, Inc. Processes and systems for preparing nucleic acid sequencing libraries and libraries prepared using same
GB2531616B (en) * 2015-02-02 2017-11-22 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB201501705D0 (en) 2015-02-02 2015-03-18 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
GB2531615B (en) * 2015-02-02 2017-11-22 Atlas Genetics Ltd Instrument for performing a diagnostic test on a fluidic cartridge
WO2016145409A1 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Genotype and phenotype coupling
SG10201811548PA (en) * 2015-08-26 2019-02-27 Emulate Inc Perfusion Manifold Assembly
WO2017075265A1 (en) 2015-10-28 2017-05-04 The Broad Institute, Inc. Multiplex analysis of single cell constituents
WO2017075297A1 (en) 2015-10-28 2017-05-04 The Broad Institute Inc. High-throughput dynamic reagent delivery system
CN108463732B (en) * 2015-11-04 2022-07-01 日东电工株式会社 Apparatus and system for biological fluid sample distribution and/or assay
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
EP3397973A4 (en) * 2015-12-30 2019-06-05 Bio-Rad Laboratories, Inc. Droplet assay system with automatic calibration
US10730030B2 (en) 2016-01-08 2020-08-04 Bio-Rad Laboratories, Inc. Multiple beads per droplet resolution
US12071663B2 (en) 2016-01-15 2024-08-27 Massachusetts Institute Of Technology Semi-permeable arrays for analyzing biological systems and methods of using same
US11081208B2 (en) 2016-02-11 2021-08-03 10X Genomics, Inc. Systems, methods, and media for de novo assembly of whole genome sequence data
US10233487B2 (en) 2016-03-24 2019-03-19 Bio-Rad Laboratories, Inc. Use of gel beads to control droplet dispersion
JP6466027B2 (en) 2016-04-20 2019-02-13 シスメックス株式会社 Nucleic acid analyzer
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
USD800335S1 (en) * 2016-07-13 2017-10-17 Precision Nanosystems Inc. Microfluidic chip
CN116064727A (en) 2016-07-27 2023-05-05 斯坦福大学托管董事会 Highly multiplexed fluorescence imaging
GB201622024D0 (en) 2016-11-14 2017-02-08 Inventage Lab Inc Apparatus and method for large scale production of monodisperse, microsheric and biodegradable polymer-based drug delivery
CN110114146B (en) 2016-12-20 2021-11-19 生物辐射实验室股份有限公司 UV grafting on microfluidic devices
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
EP3562893B1 (en) 2016-12-28 2024-06-19 Bio-Rad Laboratories, Inc. Modification of surface properties of microfluidic devices
GB201704769D0 (en) 2017-01-03 2017-05-10 Illumina Inc Flowcell cartridge with floating seal bracket
US20180209874A1 (en) * 2017-01-26 2018-07-26 Bio-Rad Laboratories, Inc. Assay performance systems including aqueous sample stabilization
CN110214186B (en) 2017-01-30 2023-11-24 10X基因组学有限公司 Methods and systems for droplet-based single cell barcoding
US12264411B2 (en) 2017-01-30 2025-04-01 10X Genomics, Inc. Methods and systems for analysis
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
US11207685B2 (en) 2017-02-13 2021-12-28 Bio-Rad Laboratories, Inc. System, method, and device for forming an array of emulsions
CN106834115B (en) * 2017-02-27 2023-10-24 武汉科技大学 Microdroplet digital PCR chip module
US11072816B2 (en) 2017-05-03 2021-07-27 The Broad Institute, Inc. Single-cell proteomic assay using aptamers
US10544413B2 (en) 2017-05-18 2020-01-28 10X Genomics, Inc. Methods and systems for sorting droplets and beads
EP4435113A1 (en) 2017-05-18 2024-09-25 10x Genomics, Inc. Methods and systems for sorting droplets and beads
US10610865B2 (en) 2017-08-22 2020-04-07 10X Genomics, Inc. Droplet forming devices and system with differential surface properties
CN111295245B (en) 2017-08-29 2021-03-16 伯乐实验室有限公司 Systems and methods for separating and analyzing cells
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019083852A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Microfluidic channel networks for partitioning
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. Methods and systems for nuclecic acid preparation and chromatin analysis
EP4241882B1 (en) 2017-10-27 2025-04-30 10X Genomics, Inc. Methods for sample preparation and analysis
EP3954782A1 (en) 2017-11-15 2022-02-16 10X Genomics, Inc. Functionalized gel beads
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019110590A1 (en) 2017-12-06 2019-06-13 Samplix Aps A microfluidic device and a method for provision of double emulsion droplets
EP3720603B1 (en) 2017-12-06 2025-02-19 Samplix ApS A microfluidic device and a method for provision of emulsion droplets
CN108004136B (en) * 2017-12-07 2024-09-17 深圳市博瑞生物科技有限公司 Microfluidic droplet generation chip
CN108251269B (en) * 2017-12-08 2024-07-19 深圳市博瑞生物科技有限公司 Digital PCR droplet generation device
CN111712579B (en) 2017-12-22 2024-10-15 10X基因组学有限公司 Systems and methods for processing nucleic acid molecules from one or more cells
SG11202007686VA (en) 2018-02-12 2020-09-29 10X Genomics Inc Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
SG11202009889VA (en) 2018-04-06 2020-11-27 10X Genomics Inc Systems and methods for quality control in single cell processing
WO2019204279A1 (en) 2018-04-16 2019-10-24 Klaris Corporation Methods and apparatus for forming 2-dimensional drop arrays
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US12239981B2 (en) 2018-07-11 2025-03-04 The University Of Hong Kong Automatic microfluidic system for continuous and quantitive collection of droplets
US12188014B1 (en) 2018-07-25 2025-01-07 10X Genomics, Inc. Compositions and methods for nucleic acid processing using blocking agents
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
WO2020028882A1 (en) 2018-08-03 2020-02-06 10X Genomics, Inc. Methods and systems to minimize barcode exchange
WO2020030090A1 (en) * 2018-08-09 2020-02-13 Versitech Limited Systems for automated handling of fluid samples into microfluidic droplets for in vitro diagnostic
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020041148A1 (en) 2018-08-20 2020-02-27 10X Genomics, Inc. Methods and systems for detection of protein-dna interactions using proximity ligation
US12357989B2 (en) * 2018-08-27 2025-07-15 Zhejiang Dapu Biotechnology Co., Ltd. Co-detection and digital quantification of bioassay
US10486155B1 (en) * 2018-10-22 2019-11-26 Klaris Corporation Vacuum-loaded, droplet-generating microfluidic chips and related methods
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
FR3090406B1 (en) * 2018-12-21 2020-12-04 Seb Sa Manufacturing apparatus, mixing machine and / or receiving device for the manufacture of a composition from a mixture of formulations
US12169198B2 (en) 2019-01-08 2024-12-17 10X Genomics, Inc. Systems and methods for sample analysis
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
JP7335343B2 (en) * 2019-01-31 2023-08-29 サンプリックス エーピーエス Microfluidic device and method for delivery of emulsion droplets
CN113710352B (en) * 2019-01-31 2024-03-26 赛普公司 Microfluidic device and method for providing dual emulsion droplets
CN118979095A (en) 2019-02-12 2024-11-19 10X基因组学有限公司 Methods for processing nucleic acid molecules
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US12275993B2 (en) 2019-02-12 2025-04-15 10X Genomics, Inc. Analysis of nucleic acid sequences
US12305239B2 (en) 2019-02-12 2025-05-20 10X Genomics, Inc. Analysis of nucleic acid sequences
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
EP3966307B1 (en) 2019-05-07 2024-11-20 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
CN110339881A (en) * 2019-07-30 2019-10-18 中国科学院深圳先进技术研究院 A microfluidic droplet generation device
US12235262B1 (en) 2019-09-09 2025-02-25 10X Genomics, Inc. Methods and systems for single cell protein analysis
EP4041310A4 (en) 2019-10-10 2024-05-15 1859, Inc. Methods and systems for microfluidic screening
CN110893354B (en) * 2019-10-22 2022-04-29 长春技特生物技术有限公司 Multi-module emulsion microdroplet generation control device
CN112782418B (en) * 2019-11-01 2024-02-27 深圳迈瑞生物医疗电子股份有限公司 External liquid supply device and liquid supply method
CN110813394A (en) * 2019-11-05 2020-02-21 中国科学院苏州生物医学工程技术研究所 Droplet generating chip mounting device
CN110760438B (en) * 2019-11-05 2024-11-19 苏州中科医疗器械产业发展有限公司 Droplet generation device
WO2021119201A1 (en) 2019-12-10 2021-06-17 Enumerix, Inc. Methods and systems for three-dimensional lightsheet imaging
US11504719B2 (en) 2020-03-12 2022-11-22 Bio-Rad Laboratories, Inc. System and method for receiving and delivering a fluid for sample processing
US10953404B1 (en) 2020-04-24 2021-03-23 Pattern Bioscience, Inc. Apparatuses for contactless loading and imaging of microfluidic chips and related methods
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
WO2022109721A1 (en) * 2020-11-30 2022-06-02 Precision Nanosystems Inc. Non aggregating microfluidic mixer and methods therefor
WO2022157916A1 (en) * 2021-01-22 2022-07-28 株式会社エンプラス Liquid handling system
WO2022182682A1 (en) 2021-02-23 2022-09-01 10X Genomics, Inc. Probe-based analysis of nucleic acids and proteins
CN117241878A (en) 2021-03-05 2023-12-15 伊努梅里斯公司 Systems and methods for generating droplets and performing digital analysis
JP7585960B2 (en) 2021-05-14 2024-11-19 東ソー株式会社 Method for generating emulsions in microfluidic chips
US12252745B2 (en) 2021-09-02 2025-03-18 Enumerix, Inc. Detection and digital quantitation of multiple targets
EP4453246A1 (en) 2021-12-20 2024-10-30 Enumerix, Inc. Detection and digital quantitation of multiple targets
CN114733452B (en) * 2022-03-27 2022-12-23 浙江大学 A kind of preparation device of graphene airgel block
US20240198336A1 (en) 2022-12-19 2024-06-20 Anchorline Biolabs, Inc. Capillary partitioning microfluidics
CN118496966B (en) * 2024-06-20 2025-02-28 逐因生物科技(重庆)有限公司 Gas supply component, single cell sequencing library building equipment and control method thereof

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007133710A2 (en) * 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof

Family Cites Families (278)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3575220A (en) 1968-08-12 1971-04-20 Scientific Industries Apparatus for dispensing liquid sample
BR7500831A (en) 1974-02-11 1975-12-02 Fmc Corp GAS BUBBLE SEPARATING APPLIANCE GAS TRANSMISSION BODY FOR USE IN THE PROCESS APPLIANCE FOR THE MANUFACTURING OF THAT BODY AND FOR DIFFUSING FINE GAS BUBBLES INTO A BODY OF LIQUID
US4051025A (en) 1976-09-29 1977-09-27 The United States Of America As Represented By The Department Of Health, Education And Welfare Preparative countercurrent chromatography with a slowly rotating helical tube array
US4201691A (en) 1978-01-16 1980-05-06 Exxon Research & Engineering Co. Liquid membrane generator
US4348111A (en) 1978-12-07 1982-09-07 The English Electric Company Limited Optical particle analyzers
US4283262A (en) 1980-07-01 1981-08-11 Instrumentation Laboratory Inc. Analysis system
GB2097692B (en) 1981-01-10 1985-05-22 Shaw Stewart P D Combining chemical reagents
US4399219A (en) 1981-01-29 1983-08-16 Massachusetts Institute Of Technology Process for isolating microbiologically active material
US4636075A (en) 1984-08-22 1987-01-13 Particle Measuring Systems, Inc. Particle measurement utilizing orthogonally polarized components of a laser beam
US4948961A (en) 1985-08-05 1990-08-14 Biotrack, Inc. Capillary flow device
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
US5055390A (en) 1988-04-22 1991-10-08 Massachusetts Institute Of Technology Process for chemical manipulation of non-aqueous surrounded microdroplets
WO1990015807A1 (en) 1989-06-22 1990-12-27 Applications Et Transferts De Technologies Avancées Fluorine and phosphorous-containing amphiphilic molecules with surfactant properties
GB8917963D0 (en) 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
GB9016163D0 (en) 1990-07-24 1990-09-05 Cemu Bioteknik Chemical method
KR100236506B1 (en) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 Apparatus for polymerase chain reaction
US5270183A (en) 1991-02-08 1993-12-14 Beckman Research Institute Of The City Of Hope Device and method for the automated cycling of solutions between two or more temperatures
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
ES2091976T3 (en) 1991-06-20 1996-11-16 Hoffmann La Roche PERFECTED METHODS FOR THE AMPLIFICATION OF NUCLEIC ACID.
US5422277A (en) 1992-03-27 1995-06-06 Ortho Diagnostic Systems Inc. Cell fixative composition and method of staining cells without destroying the cell surface
US5637469A (en) 1992-05-01 1997-06-10 Trustees Of The University Of Pennsylvania Methods and apparatus for the detection of an analyte utilizing mesoscale flow systems
US5587128A (en) 1992-05-01 1996-12-24 The Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification devices
US5639423A (en) 1992-08-31 1997-06-17 The Regents Of The University Of Calfornia Microfabricated reactor
JP2504059Y2 (en) * 1992-10-01 1996-07-03 石川ガスケット株式会社 Head gasket
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
US5538667A (en) 1993-10-28 1996-07-23 Whitehill Oral Technologies, Inc. Ultramulsions
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
CA2159830C (en) 1994-04-29 2001-07-03 Timothy M Woudenberg System for real time detection of nucleic acid amplification products
US5972716A (en) 1994-04-29 1999-10-26 The Perkin-Elmer Corporation Fluorescence monitoring device with textured optical tube and method for reducing background fluorescence
DE69527585T2 (en) 1994-06-08 2003-04-03 Affymetrix, Inc. Method and device for packaging chips
US5555191A (en) 1994-10-12 1996-09-10 Trustees Of Columbia University In The City Of New York Automated statistical tracker
FI100859B (en) 1994-10-14 1998-03-13 Bjarne Holmbom Method and apparatus for performing on-line flow extraction of extractable components in liquids
US5585069A (en) 1994-11-10 1996-12-17 David Sarnoff Research Center, Inc. Partitioned microelectronic and fluidic device array for clinical diagnostics and chemical synthesis
FR2733766B1 (en) 1995-05-03 1997-06-06 Rhone Poulenc Rorer Sa SCHIZOPHRENIA DIAGNOSIS METHOD
US20020022261A1 (en) 1995-06-29 2002-02-21 Anderson Rolfe C. Miniaturized genetic analysis systems and methods
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US6057149A (en) 1995-09-15 2000-05-02 The University Of Michigan Microscale devices and reactions in microscale devices
US20020068357A1 (en) 1995-09-28 2002-06-06 Mathies Richard A. Miniaturized integrated nucleic acid processing and analysis device and method
US6562605B1 (en) 1995-11-13 2003-05-13 Genencor International, Inc. Extraction of water soluble biomaterials from fluids using a carbon dioxide/surfactant mixture
US5736314A (en) 1995-11-16 1998-04-07 Microfab Technologies, Inc. Inline thermo-cycler
CA2250212C (en) 1996-04-03 2010-02-09 The Perkin-Elmer Corporation Device and method for multiple analyte detection
JP3788519B2 (en) 1996-06-28 2006-06-21 カリパー・ライフ・サイエンシズ・インコーポレーテッド High-throughput screening assay system for microscale fluid devices
CN1329729C (en) 1996-06-28 2007-08-01 卡钳生命科学股份有限公司 Electropipettor and compensation means for electrophoretic bias
US7026468B2 (en) 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US6558916B2 (en) 1996-08-02 2003-05-06 Axiom Biotechnologies, Inc. Cell flow apparatus and method for real-time measurements of patient cellular responses
GB9621357D0 (en) 1996-10-12 1996-12-04 Central Research Lab Ltd Heating apparatus
JPH10202091A (en) * 1997-01-23 1998-08-04 Osaka Gas Co Ltd Production of organic fine sphere and carbonaceous fine sphere
US7268179B2 (en) 1997-02-03 2007-09-11 Cytonix Corporation Hydrophobic coating compositions, articles coated with said compositions, and processes for manufacturing same
EP0972081B1 (en) 1997-04-01 2007-06-13 Solexa Ltd. Method of nucleic acid amplification
ATE269908T1 (en) 1997-04-01 2004-07-15 Manteia S A METHOD FOR SEQUENCING NUCLEIC ACIDS
US6391622B1 (en) 1997-04-04 2002-05-21 Caliper Technologies Corp. Closed-loop biochemical analyzers
US6143496A (en) 1997-04-17 2000-11-07 Cytonix Corporation Method of sampling, amplifying and quantifying segment of nucleic acid, polymerase chain reaction assembly having nanoliter-sized sample chambers, and method of filling assembly
ATE264717T1 (en) 1997-05-16 2004-05-15 Alberta Res Council MICROFLUIDIC SYSTEM AND METHOD FOR OPERATING THEREOF
US6664044B1 (en) 1997-06-19 2003-12-16 Toyota Jidosha Kabushiki Kaisha Method for conducting PCR protected from evaporation
US5912945A (en) 1997-06-23 1999-06-15 Regents Of The University Of California X-ray compass for determining device orientation
DE69841997D1 (en) 1997-07-07 2010-12-23 Medical Res Council London In vitro sorting method
US5980936A (en) 1997-08-07 1999-11-09 Alliance Pharmaceutical Corp. Multiple emulsions comprising a hydrophobic continuous phase
ATE294860T1 (en) 1997-09-16 2005-05-15 Egea Biosciences Llc METHODS FOR COMPLETE CHEMICAL SYNTHESIS AND ASSEMBLY OF GENES AND GENOMES
US6833242B2 (en) 1997-09-23 2004-12-21 California Institute Of Technology Methods for detecting and sorting polynucleotides based on size
US6540895B1 (en) 1997-09-23 2003-04-01 California Institute Of Technology Microfabricated cell sorter for chemical and biological materials
US6074725A (en) 1997-12-10 2000-06-13 Caliper Technologies Corp. Fabrication of microfluidic circuits by printing techniques
ES2277430T3 (en) 1998-03-04 2007-07-01 Visx Incorporated LASER PRESBORAGE TREATMENT SYSTEM.
US6384915B1 (en) 1998-03-30 2002-05-07 The Regents Of The University Of California Catheter guided by optical coherence domain reflectometry
JP3081880B2 (en) 1998-03-30 2000-08-28 農林水産省食品総合研究所長 Microsphere continuous manufacturing equipment
US6175669B1 (en) 1998-03-30 2001-01-16 The Regents Of The Universtiy Of California Optical coherence domain reflectometry guidewire
JP3012608B1 (en) 1998-09-17 2000-02-28 農林水産省食品総合研究所長 Microchannel device and method for producing emulsion using the same
US6146103A (en) 1998-10-09 2000-11-14 The Regents Of The University Of California Micromachined magnetohydrodynamic actuators and sensors
US6637463B1 (en) 1998-10-13 2003-10-28 Biomicro Systems, Inc. Multi-channel microfluidic system design with balanced fluid flow distribution
US6176609B1 (en) 1998-10-13 2001-01-23 V & P Scientific, Inc. Magnetic tumble stirring method, devices and machines for mixing in vessels
US6086740A (en) 1998-10-29 2000-07-11 Caliper Technologies Corp. Multiplexed microfluidic devices and systems
US6261431B1 (en) 1998-12-28 2001-07-17 Affymetrix, Inc. Process for microfabrication of an integrated PCR-CE device and products produced by the same
GB9900298D0 (en) 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
US6522407B2 (en) 1999-01-22 2003-02-18 The Regents Of The University Of California Optical detection dental disease using polarized light
US6326083B1 (en) 1999-03-08 2001-12-04 Calipher Technologies Corp. Surface coating for microfluidic devices that incorporate a biopolymer resistant moiety
US6303343B1 (en) 1999-04-06 2001-10-16 Caliper Technologies Corp. Inefficient fast PCR
WO2000061808A2 (en) 1999-04-09 2000-10-19 Exact Laboratories, Inc. Methods for detecting nucleic acids indicative of cancer
US6357907B1 (en) 1999-06-15 2002-03-19 V & P Scientific, Inc. Magnetic levitation stirring devices and machines for mixing in vessels
WO2001007159A2 (en) 1999-07-28 2001-02-01 Genset Integration of biochemical protocols in a continuous flow microfluidic device
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
US6440706B1 (en) 1999-08-02 2002-08-27 Johns Hopkins University Digital amplification
US6524456B1 (en) 1999-08-12 2003-02-25 Ut-Battelle, Llc Microfluidic devices for the controlled manipulation of small volumes
DE19947495C2 (en) 1999-10-01 2003-05-28 Agilent Technologies Inc Microfluidic microchip
WO2001042781A2 (en) 1999-12-07 2001-06-14 Exact Sciences Corporation Supracolonic aerodigestive neoplasm detection
WO2001049414A2 (en) 2000-01-06 2001-07-12 Caliper Technologies Corp. Ultra high throughput sampling and analysis systems and methods
US20020058329A1 (en) 2000-02-18 2002-05-16 Sharat Singh Multiple-site reaction device and method
US20020151040A1 (en) 2000-02-18 2002-10-17 Matthew O' Keefe Apparatus and methods for parallel processing of microvolume liquid reactions
JP3282619B2 (en) 2000-03-22 2002-05-20 住友電装株式会社 Wire harness bending test method and wire bending test apparatus
WO2001090614A2 (en) 2000-05-24 2001-11-29 Micronics, Inc. Surface tension valves for microfluidic applications
US7351376B1 (en) 2000-06-05 2008-04-01 California Institute Of Technology Integrated active flux microfluidic devices and methods
US6466713B2 (en) 2000-08-18 2002-10-15 The Regents Of The University Of California Optical fiber head for providing lateral viewing
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US6670153B2 (en) 2000-09-14 2003-12-30 Caliper Technologies Corp. Microfluidic devices and methods for performing temperature mediated reactions
US7294503B2 (en) 2000-09-15 2007-11-13 California Institute Of Technology Microfabricated crossflow devices and methods
JP4015946B2 (en) 2000-10-30 2007-11-28 シークエノム・インコーポレーテツド Method and apparatus for supplying sub-microliter volumes on a substrate
KR100435921B1 (en) 2000-12-29 2004-06-12 주식회사 태평양 A stable water-in-oil-in-water multiple emulsion system by hydrodynamic dual stabilization and a method thereof
WO2002081490A2 (en) 2001-01-19 2002-10-17 Egea Biosciences, Inc. Computer-directed assembly of a polynucleotide encoding a target polypeptide
US7567596B2 (en) 2001-01-30 2009-07-28 Board Of Trustees Of Michigan State University Control system and apparatus for use with ultra-fast laser
DE60221036T2 (en) 2001-02-23 2007-10-11 Japan Science And Technology Agency, Kawaguchi Device for producing emulsions and microcapsules
WO2002069016A2 (en) 2001-02-28 2002-09-06 Lightwave Microsystems Corporation Microfluid control for waveguide optical switches, variable attenuators, and other optical devices
US7270786B2 (en) 2001-03-28 2007-09-18 Handylab, Inc. Methods and systems for processing microfluidic samples of particle containing fluids
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US6575188B2 (en) 2001-07-26 2003-06-10 Handylab, Inc. Methods and systems for fluid control in microfluidic devices
US7192557B2 (en) 2001-03-28 2007-03-20 Handylab, Inc. Methods and systems for releasing intracellular material from cells within microfluidic samples of fluids
EP1384022A4 (en) 2001-04-06 2004-08-04 California Inst Of Techn AMPLIFICATION OF NUCLEIC ACID USING MICROFLUIDIC DEVICES
US6793723B2 (en) 2001-05-10 2004-09-21 Pitney Bowes Inc. Homogeneous photosensitive optically variable ink compositions for ink jet printing
US20030049659A1 (en) 2001-05-29 2003-03-13 Lapidus Stanley N. Devices and methods for isolating samples into subsamples for analysis
EP1406640A2 (en) 2001-06-12 2004-04-14 Keryx Biopharmceuticals Inc. Use of glycosaminoglycans for the treatment of hiv-associated nephropathy
US6905885B2 (en) 2001-06-12 2005-06-14 The Regents Of The University Of California Portable pathogen detection system
US6808683B2 (en) 2001-09-25 2004-10-26 Cytonome, Inc. Droplet dispensing system
JP4537053B2 (en) 2001-06-25 2010-09-01 ジョージア テック リサーチ コーポレーション Double resonance energy transfer nucleic acid probe
US6797257B2 (en) 2001-06-26 2004-09-28 The Board Of Trustees Of The University Of Illinois Paramagnetic polymerized protein microspheres and methods of preparation thereof
US6573490B2 (en) 2001-06-28 2003-06-03 Valeo Electrical Systems, Inc. Interleaved mosaic imaging rain sensor
US20030032172A1 (en) 2001-07-06 2003-02-13 The Regents Of The University Of California Automated nucleic acid assay system
US7297778B2 (en) 2001-07-25 2007-11-20 Affymetrix, Inc. Complexity management of genomic DNA
US20030027150A1 (en) 2001-08-03 2003-02-06 Katz David A. Method of haplotyping and kit therefor
AUPR707101A0 (en) 2001-08-16 2001-09-06 Corbett Research Pty Ltd Continuous flow thermal device
US7094379B2 (en) 2001-10-24 2006-08-22 Commissariat A L'energie Atomique Device for parallel and synchronous injection for sequential injection of different reagents
KR100442836B1 (en) 2001-11-10 2004-08-02 삼성전자주식회사 System and method for circulating biochemical fluidic solutions around closed two or more temperature zones of chambers
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
US7118910B2 (en) 2001-11-30 2006-10-10 Fluidigm Corporation Microfluidic device and methods of using same
US7198897B2 (en) 2001-12-19 2007-04-03 Brandeis University Late-PCR
US20030170698A1 (en) 2002-01-04 2003-09-11 Peter Gascoyne Droplet-based microfluidic oligonucleotide synthesis engine
US20030180765A1 (en) 2002-02-01 2003-09-25 The Johns Hopkins University Digital amplification for detection of mismatch repair deficient tumor cells
AU2003219870A1 (en) 2002-02-22 2003-09-09 Biodot, Inc. Method and apparatus for dispersing reagent droplets below a fluid surface using non-contact dispensing
EP1488006B1 (en) 2002-03-20 2008-05-28 InnovativeBio.Biz Microcapsules with controlable permeability encapsulating a nucleic acid amplification reaction mixture and their use as reaction compartments for parallels reactions
US7312085B2 (en) 2002-04-01 2007-12-25 Fluidigm Corporation Microfluidic particle-analysis systems
US7993266B2 (en) 2002-04-26 2011-08-09 Lawrence Livermore National Security, Llc Early detection of contagious diseases
ATE479899T1 (en) 2002-05-09 2010-09-15 Univ Chicago EQUIPMENT AND METHODS FOR PRESSURE CONTROLLED PLUG TRANSPORT AND REACTION
FR2841063B1 (en) 2002-06-18 2004-09-17 Commissariat Energie Atomique DEVICE FOR DISPLACING SMALL VOLUMES OF LIQUID ALONG A MICRO-CATENARY BY ELECTROSTATIC FORCES
JP2006507921A (en) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Method and apparatus for fluid dispersion
US6794671B2 (en) 2002-07-17 2004-09-21 Particle Sizing Systems, Inc. Sensors and methods for high-sensitivity optical particle counting and sizing
US7188731B2 (en) 2002-08-26 2007-03-13 The Regents Of The University Of California Variable flexure-based fluid filter
US20040038385A1 (en) 2002-08-26 2004-02-26 Langlois Richard G. System for autonomous monitoring of bioagents
US8871446B2 (en) 2002-10-02 2014-10-28 California Institute Of Technology Microfluidic nucleic acid analysis
US20050042639A1 (en) 2002-12-20 2005-02-24 Caliper Life Sciences, Inc. Single molecule amplification and detection of DNA length
US20060094108A1 (en) 2002-12-20 2006-05-04 Karl Yoder Thermal cycler for microfluidic array assays
CA2510166A1 (en) 2002-12-20 2004-09-30 Caliper Life Sciences, Inc. Single molecule amplification and detection of dna
US7682565B2 (en) 2002-12-20 2010-03-23 Biotrove, Inc. Assay apparatus and method using microfluidic arrays
CA2513889A1 (en) 2003-01-29 2004-08-19 454 Corporation Double ended sequencing
US7595195B2 (en) 2003-02-11 2009-09-29 The Regents Of The University Of California Microfluidic devices for controlled viscous shearing and formation of amphiphilic vesicles
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
EP2306174B1 (en) 2003-03-28 2016-05-11 Inguran, LLC Flow cytometry nozzle for orienting particles and corresponding method
US7604965B2 (en) 2003-04-03 2009-10-20 Fluidigm Corporation Thermal reaction device and method for using the same
US8828663B2 (en) 2005-03-18 2014-09-09 Fluidigm Corporation Thermal reaction device and method for using the same
DE10315640A1 (en) 2003-04-04 2004-10-14 Ignatov, Konstantin Process for the controlled release of components into a solution
EP1685282A2 (en) 2003-04-17 2006-08-02 Fluidigm Corporation Crystal growth devices and systems, and methods for using same
US7629123B2 (en) 2003-07-03 2009-12-08 University Of Medicine And Dentistry Of New Jersey Compositions and methods for diagnosing autism
ATE478963T1 (en) 2003-07-03 2010-09-15 Univ New Jersey Med GENES AS DIAGNOSTIC TOOLS FOR AUTISM
EP1641809B2 (en) 2003-07-05 2018-10-03 The Johns Hopkins University Method and compositions for detection and enumeration of genetic variations
AU2004262406B2 (en) 2003-08-01 2008-07-03 Ge Healthcare Bio-Sciences Ab Optical resonance analysis unit
KR20070029618A (en) 2003-08-27 2007-03-14 더 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 Electronic control of fluid species
EP2354253A3 (en) 2003-09-05 2011-11-16 Trustees of Boston University Method for non-invasive prenatal diagnosis
WO2005043154A2 (en) 2003-10-27 2005-05-12 Massachusetts Institute Of Technology High density reaction chambers and methods of use
US7141537B2 (en) 2003-10-30 2006-11-28 3M Innovative Properties Company Mixture of fluorinated polyethers and use thereof as surfactant
WO2005052578A1 (en) * 2003-11-28 2005-06-09 Olympus Corporation Biosubstance testing apparatus and reaction stage thereof
US20070248956A1 (en) 2003-12-05 2007-10-25 Buxbaum Joseph D Methods and Compositions for Autism Risk Assessment
EP1735458B1 (en) 2004-01-28 2013-07-24 454 Life Sciences Corporation Nucleic acid amplification with continuous flow emulsion
US8293471B2 (en) 2004-01-28 2012-10-23 Marshall University Research Corporation Apparatus and method for a continuous rapid thermal cycle system
WO2005075683A1 (en) 2004-02-03 2005-08-18 Postech Foundation High throughput device for performing continuous-flow reactions
KR100552706B1 (en) 2004-03-12 2006-02-20 삼성전자주식회사 Nucleic Acid Amplification Method and Apparatus
CA2560272C (en) 2004-03-23 2012-05-08 Japan Science And Technology Agency Method and apparatus for producing microdroplets
US7432106B2 (en) 2004-03-24 2008-10-07 Applied Biosystems Inc. Liquid processing device including gas trap, and system and method
JP4350148B2 (en) 2004-03-31 2009-10-21 ザ ジェネラル ホスピタル コーポレイション Methods for determining cancer responsiveness to epidermal growth factor receptor targeting therapy
US20050221279A1 (en) 2004-04-05 2005-10-06 The Regents Of The University Of California Method for creating chemical sensors using contact-based microdispensing technology
HU227246B1 (en) 2004-04-30 2010-12-28 Szegedi Biolog Koezpont Use of genes as molecular markers in diagnosis of schizophrenia and diagnostic kit
US20070231393A1 (en) 2004-05-19 2007-10-04 University Of South Carolina System and Device for Magnetic Drug Targeting with Magnetic Drug Carrier Particles
WO2006002167A2 (en) 2004-06-17 2006-01-05 University Of Florida Research Foudation, Inc. Multi-acceptor molecular probes and applications thereof
EP1612279B1 (en) 2004-06-29 2009-03-11 Roche Diagnostics GmbH Association of single nucleotide polymorphisms in PPAR gamma with osteoporosis
WO2006023719A2 (en) 2004-08-20 2006-03-02 Enh Research Institute Identification of snp’s associated with schizophrenia, schizoaffective disorder and bipolar disorder
ES2784184T3 (en) 2004-09-09 2020-09-23 Inst Curie Microfluidic device that uses a collinear electric field
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
US9109256B2 (en) 2004-10-27 2015-08-18 Esoterix Genetic Laboratories, Llc Method for monitoring disease progression or recurrence
US8951746B2 (en) 2004-11-05 2015-02-10 Southwest Research Institute Method and devices for screening cervical cancer
US20090098044A1 (en) 2004-11-15 2009-04-16 Australian Nuclear Science And Technology Organisation Solid particles from controlled destabilisation of microemulsions
US20060166223A1 (en) 2005-01-26 2006-07-27 Reed Michael W DNA purification and analysis on nanoengineered surfaces
WO2006086382A2 (en) 2005-02-08 2006-08-17 Northrop Grumman Corporation System and methods for use in detecting harmful aerosol particles
EP1851339B1 (en) 2005-02-11 2016-05-18 Memorial Sloan-Kettering Cancer Center Methods and compositions for detecting a drug resistant egfr mutant
AU2006220816A1 (en) 2005-03-04 2006-09-14 President And Fellows Of Harvard College Method and apparatus for forming multiple emulsions
US20070054119A1 (en) * 2005-03-04 2007-03-08 Piotr Garstecki Systems and methods of forming particles
RU2400538C2 (en) 2005-03-05 2010-09-27 Сиджен, Инк. Oligonucleotide with double specificity and methods, which it is used in
WO2006099579A2 (en) 2005-03-16 2006-09-21 Applera Corporation Compositions and methods for clonal amplification and analysis of polynucleotides
US20070048756A1 (en) 2005-04-18 2007-03-01 Affymetrix, Inc. Methods for whole genome association studies
WO2006114596A1 (en) * 2005-04-26 2006-11-02 Aviza Technology Limited Microfluidic structures and how to make them
DE102005034642B3 (en) * 2005-07-25 2006-08-03 Siemens Ag Laboratory microfluidic analytical mixer unit has two separate passage systems, one of which has internal leak detector
DE102005037401B4 (en) * 2005-08-08 2007-09-27 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Formation of an emulsion in a fluidic microsystem
AU2006294565A1 (en) 2005-09-30 2007-04-05 Perlegen Sciences, Inc. Methods and compositions for screening and treatment of disorders of blood glucose regulation
EP3913375A1 (en) 2006-01-11 2021-11-24 Bio-Rad Laboratories, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
US7976795B2 (en) * 2006-01-19 2011-07-12 Rheonix, Inc. Microfluidic systems
WO2007089541A2 (en) 2006-01-27 2007-08-09 President And Fellows Of Harvard College Fluidic droplet coalescence
EP2423334A3 (en) 2006-02-02 2012-04-18 The Board of Trustees of The Leland Stanford Junior University Non-invasive fetal genetic screening by digital analysis
US20080280331A1 (en) 2006-02-07 2008-11-13 Stokes Bio Limited Microfluidic Analysis System
US8298833B2 (en) 2006-02-07 2012-10-30 Stokes Bio Limited Liquid bridge and system
US20100304446A1 (en) 2006-02-07 2010-12-02 Stokes Bio Limited Devices, systems, and methods for amplifying nucleic acids
WO2007120745A2 (en) 2006-04-11 2007-10-25 Optiscan Biomedical Corporation Noise reduction for analyte detection system
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
US7816121B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
WO2007124409A2 (en) * 2006-04-20 2007-11-01 Velocys, Inc. Process for treating and/or forming a non-newtonian fluid using microchannel process technology
JP2009537652A (en) * 2006-05-18 2009-10-29 マリリン，レイナー Membrane manufacturing method and membrane for emulsification
EP2041318A2 (en) 2006-06-26 2009-04-01 Blood Cell Storage, Inc. Device and method for extraction and analysis of nucleic acids from biological samples
EP1881079A1 (en) 2006-07-20 2008-01-23 Pangaea Biotech, S.A. Method for the detection of EGFR mutations in blood samples
WO2008021123A1 (en) 2006-08-07 2008-02-21 President And Fellows Of Harvard College Fluorocarbon emulsion stabilizing surfactants
WO2008024114A1 (en) 2006-08-24 2008-02-28 Genizon Biosciences Inc. Genemap of the human genes associated with schizophrenia
JP2008080306A (en) * 2006-09-29 2008-04-10 Hitachi Ltd Chemical synthesis equipment
EP1912067A1 (en) 2006-10-12 2008-04-16 Eppendorf Array Technologies S.A. Method for quantification of a target compound obtained from a biological sample upon chips
US8841116B2 (en) 2006-10-25 2014-09-23 The Regents Of The University Of California Inline-injection microdevice and microfabricated integrated DNA analysis system using same
WO2008070074A2 (en) 2006-12-04 2008-06-12 Pgxhealth Llc Genetic markers of schizophrenia
US20080176237A1 (en) 2006-12-07 2008-07-24 Biocept, Inc. Non-invasive prenatal genetic screen
US8262900B2 (en) 2006-12-14 2012-09-11 Life Technologies Corporation Methods and apparatus for measuring analytes using large scale FET arrays
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008089244A2 (en) 2007-01-17 2008-07-24 University Of Rochester Frequency-addressable apparatus and methods for actuation of liquids
WO2008109878A2 (en) 2007-03-07 2008-09-12 California Institute Of Technology Testing device
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
US20100144538A1 (en) 2007-03-08 2010-06-10 Genizon Biosciences Inc. Genemap of the human genes associated with schizophrenia
US7776927B2 (en) 2007-03-28 2010-08-17 President And Fellows Of Harvard College Emulsions and techniques for formation
JP5666763B2 (en) 2007-04-11 2015-02-12 味の素株式会社 Water-in-oil emulsion composition
EP2162205A1 (en) * 2007-06-05 2010-03-17 Eugenia Kumacheva Multiple continuous microfluidic reactors for the scaled up synthesis of gel or polymer particles
WO2009002920A1 (en) 2007-06-22 2008-12-31 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
WO2009011808A1 (en) 2007-07-13 2009-01-22 President And Fellows Of Harvard College Droplet-based selection
CA2694820C (en) 2007-07-30 2015-06-16 F. Hoffman-La Roche Ag Methods of identification using methylation of cpg
US20090053719A1 (en) 2007-08-03 2009-02-26 The Chinese University Of Hong Kong Analysis of nucleic acids by digital pcr
CA2698545C (en) 2007-09-07 2014-07-08 Fluidigm Corporation Copy number variation determination, methods and systems
EP2203567B1 (en) 2007-10-16 2011-05-11 Roche Diagnostics GmbH High resolution, high throughput hla genotyping by clonal sequencing
US7807920B2 (en) 2007-10-30 2010-10-05 Opel, Inc. Concentrated solar photovoltaic module
CN101855007B (en) * 2007-11-09 2014-06-18 M技术株式会社 Method of producing emulsion and emulsion obtained thereby
US8603566B2 (en) 2007-12-20 2013-12-10 University Of Massachusetts Cross-linked biopolymers, related compounds and methods of use
JP5224801B2 (en) 2007-12-21 2013-07-03 キヤノン株式会社 Nucleic acid amplification equipment
US8367370B2 (en) 2008-02-11 2013-02-05 Wheeler Aaron R Droplet-based cell culture and cell assays using digital microfluidics
US20090220434A1 (en) 2008-02-29 2009-09-03 Florida State University Research Foundation Nanoparticles that facilitate imaging of biological tissue and methods of forming the same
US8062903B2 (en) 2008-03-03 2011-11-22 University Of Washington Droplet compartmentalization for chemical separation and on-line sampling
US9487822B2 (en) 2008-03-19 2016-11-08 Fluidigm Corporation Method and apparatus for determining copy number variation using digital PCR
US9011777B2 (en) * 2008-03-21 2015-04-21 Lawrence Livermore National Security, Llc Monodisperse microdroplet generation and stopping without coalescence
EP2672260A1 (en) 2008-05-13 2013-12-11 Advanced Liquid Logic, Inc. Droplet actuator devices, systems and methods
CA2729856A1 (en) 2008-07-04 2010-01-07 Decode Genetics Ehf. Copy number variations predictive of risk of schizophrenia
US8256931B2 (en) 2008-07-24 2012-09-04 Seward George H Achromatic homogenizer and collimator for LEDs
EP4008795A1 (en) 2008-08-12 2022-06-08 Stokes Bio Limited Methods for digital pcr
US9180453B2 (en) 2008-08-15 2015-11-10 University Of Washington Method and apparatus for the discretization and manipulation of sample volumes
WO2010021936A1 (en) 2008-08-16 2010-02-25 The Board Of Trustees Of The Leland Stanford Junior University Digital pcr calibration for high throughput sequencing
US8383345B2 (en) 2008-09-12 2013-02-26 University Of Washington Sequence tag directed subassembly of short sequencing reads into long sequencing reads
US20110218123A1 (en) 2008-09-19 2011-09-08 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US9222128B2 (en) 2011-03-18 2015-12-29 Bio-Rad Laboratories, Inc. Multiplexed digital assays with combinatorial use of signals
US9156010B2 (en) 2008-09-23 2015-10-13 Bio-Rad Laboratories, Inc. Droplet-based assay system
US9132394B2 (en) 2008-09-23 2015-09-15 Bio-Rad Laboratories, Inc. System for detection of spaced droplets
US20130084572A1 (en) 2011-09-30 2013-04-04 Quantalife, Inc. Calibrations and controls for droplet-based assays
US9598725B2 (en) 2010-03-02 2017-03-21 Bio-Rad Laboratories, Inc. Emulsion chemistry for encapsulated droplets
US8951939B2 (en) 2011-07-12 2015-02-10 Bio-Rad Laboratories, Inc. Digital assays with multiplexed detection of two or more targets in the same optical channel
US20120171683A1 (en) 2010-03-02 2012-07-05 Ness Kevin D Analysis of fragmented genomic dna in droplets
US20130059754A1 (en) 2011-09-01 2013-03-07 Bio-Rad Laboratories, Inc. Digital assays with reduced measurement uncertainty
US8709762B2 (en) 2010-03-02 2014-04-29 Bio-Rad Laboratories, Inc. System for hot-start amplification via a multiple emulsion
WO2011120024A1 (en) 2010-03-25 2011-09-29 Quantalife, Inc. Droplet generation for droplet-based assays
AU2009297108B2 (en) * 2008-09-23 2015-02-12 Bio-Rad Laboratories, Inc. Droplet-based assay system
US8633015B2 (en) 2008-09-23 2014-01-21 Bio-Rad Laboratories, Inc. Flow-based thermocycling system with thermoelectric cooler
CN201371054Y (en) * 2009-01-13 2009-12-30 赫培远 Multifunctional micro-fluid mechanical system
US20100298453A1 (en) 2009-01-26 2010-11-25 Invista North America S.A R.L. Board stock foam having biobased content
WO2010111231A1 (en) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation of microfluidic droplets
WO2010117457A2 (en) 2009-04-08 2010-10-14 Applied Biosystems, Llc System comprising dual-sided thermal cycler and emulsion pcr in pouch
JP5212313B2 (en) * 2009-08-24 2013-06-19 株式会社日立プラントテクノロジー Emulsifying device
CA3106547C (en) 2009-09-02 2022-07-05 Bio-Rad Laboratories, Inc. System for mixing fluids by coalescence of multiple emulsions
EP3067112B1 (en) 2009-09-21 2018-12-26 Akonni Biosystems Magnetic lysis method and device
EP2486409A1 (en) 2009-10-09 2012-08-15 Universite De Strasbourg Labelled silica-based nanomaterial with enhanced properties and uses thereof
EP2957641B1 (en) 2009-10-15 2017-05-17 Ibis Biosciences, Inc. Multiple displacement amplification
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
US10837883B2 (en) 2009-12-23 2020-11-17 Bio-Rad Laboratories, Inc. Microfluidic systems and methods for reducing the exchange of molecules between droplets
EP4484577A3 (en) 2010-02-12 2025-03-26 Bio-Rad Laboratories, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
EP2550351A4 (en) 2010-03-25 2014-07-09 Quantalife Inc Detection system for droplet-based assays
CA2767114A1 (en) 2010-03-25 2011-09-29 Bio-Rad Laboratories, Inc. Droplet transport system for detection
EP3447155A1 (en) 2010-09-30 2019-02-27 Raindance Technologies, Inc. Sandwich assays in droplets
EP2673614B1 (en) 2011-02-11 2018-08-01 Raindance Technologies, Inc. Method for forming mixed droplets
US9266104B2 (en) 2011-02-11 2016-02-23 Raindance Technologies, Inc. Thermocycling device for nucleic acid amplification and methods of use
US9150852B2 (en) 2011-02-18 2015-10-06 Raindance Technologies, Inc. Compositions and methods for molecular labeling
WO2012167142A2 (en) 2011-06-02 2012-12-06 Raindance Technolgies, Inc. Enzyme quantification
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
EP2732386A1 (en) 2011-07-13 2014-05-21 Bio-Rad Laboratories, Inc. Computation of real-world error using meta-analysis of replicates
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
EP2737089B1 (en) 2011-07-29 2017-09-06 Bio-rad Laboratories, Inc. Library characterization by digital assay

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007133710A2 (en) * 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
"Operator's Manual Tempo nano TM LC Systems Tempo nano TM MDLC", 1 January 2005 (2005-01-01), XP055132159, Retrieved from the Internet <URL:www.eksigent.com> [retrieved on 20140729] *
"Product Note Eksigent: NanoFlow Metering System", 1 January 2010 (2010-01-01), XP055132376, Retrieved from the Internet <URL:www.eksigent.com> [retrieved on 20140730] *
BEER N R ET AL: "On-chip single-copy real-time reverse-transcription PCR in isolated picoliter droplets", ANALYTICAL CHEMISTRY, AMERICAN CHEMICAL SOCIETY, US, vol. 80, no. 6, 15 March 2008 (2008-03-15), pages 1854 - 1858, XP002575963, ISSN: 0003-2700, [retrieved on 20080216], DOI: 10.1021/AC800048K *

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