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EP2596096A4 - METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELL IN HUMAN MESODERM CELL - Google Patents

METHOD FOR INDUCING DIFFERENTIATION OF HUMAN PLURIPOTENT STEM CELL IN HUMAN MESODERM CELL

Info

Publication number
EP2596096A4
EP2596096A4 EP11809770.8A EP11809770A EP2596096A4 EP 2596096 A4 EP2596096 A4 EP 2596096A4 EP 11809770 A EP11809770 A EP 11809770A EP 2596096 A4 EP2596096 A4 EP 2596096A4
Authority
EP
European Patent Office
Prior art keywords
human
cell
pluripotent stem
inducing differentiation
stem cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP11809770.8A
Other languages
German (de)
French (fr)
Other versions
EP2596096A1 (en
EP2596096B1 (en
Inventor
Kenji Osafune
Shin-Ichi Mae
Masatoshi Kajiwara
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Kyoto University NUC
Original Assignee
Kyoto University NUC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kyoto University NUC filed Critical Kyoto University NUC
Publication of EP2596096A1 publication Critical patent/EP2596096A1/en
Publication of EP2596096A4 publication Critical patent/EP2596096A4/en
Application granted granted Critical
Publication of EP2596096B1 publication Critical patent/EP2596096B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/5073—Stem cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/155—Bone morphogenic proteins [BMP]; Osteogenins; Osteogenic factor; Bone inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/16—Activin; Inhibin; Mullerian inhibiting substance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/40—Regulators of development
    • C12N2501/415—Wnt; Frizzeled
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/45—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from artificially induced pluripotent stem cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Reproductive Health (AREA)
  • Gynecology & Obstetrics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
EP11809770.8A 2010-07-21 2011-07-21 Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell Not-in-force EP2596096B1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US36637910P 2010-07-21 2010-07-21
US201161451825P 2011-03-11 2011-03-11
PCT/JP2011/067181 WO2012011610A1 (en) 2010-07-21 2011-07-21 Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell

Publications (3)

Publication Number Publication Date
EP2596096A1 EP2596096A1 (en) 2013-05-29
EP2596096A4 true EP2596096A4 (en) 2014-01-08
EP2596096B1 EP2596096B1 (en) 2018-01-31

Family

ID=45497017

Family Applications (1)

Application Number Title Priority Date Filing Date
EP11809770.8A Not-in-force EP2596096B1 (en) 2010-07-21 2011-07-21 Method for inducing differentiation of human pluripotent stem cell into intermediate mesoderm cell

Country Status (4)

Country Link
US (1) US9121011B2 (en)
EP (1) EP2596096B1 (en)
JP (1) JP5896360B2 (en)
WO (1) WO2012011610A1 (en)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2794860B1 (en) 2011-12-19 2017-05-03 Kyoto University Method for inducing differentiation of human pluripotent stem cells into intermediate mesoderm cells
JP6112733B2 (en) * 2012-04-06 2017-04-12 国立大学法人京都大学 Method for inducing erythropoietin-producing cells
KR20130119683A (en) * 2012-04-24 2013-11-01 사회복지법인 삼성생명공익재단 Culture medium for stem cells and method for culturing stem cells using the same
WO2014153548A1 (en) * 2013-03-21 2014-09-25 The Trustees Of Columbia University In The City Of New York Compositions and methods for dental tissue regeneration
WO2014168264A1 (en) * 2013-04-12 2014-10-16 国立大学法人京都大学 Method for inducing alveolar epithelium progenitor cells
ES2794074T3 (en) 2013-06-11 2020-11-17 Univ Kyoto Method to produce kidney precursor cells
AU2014280843B2 (en) 2013-06-14 2017-04-20 The University Of Queensland Renal progenitor cells
KR20160034323A (en) * 2013-07-29 2016-03-29 에프. 호프만-라 로슈 아게 Method for differentiation of pluripotent stem cells into multi-competent renal precursors
WO2015056756A1 (en) * 2013-10-18 2015-04-23 国立大学法人熊本大学 Method of inducing kidney from pluripotent stem cells
GB2548740A (en) 2014-11-25 2017-09-27 Harvard College Methods for generation of podocytes from pluripotent stem cells and cells produced by the same
AU2014277667B2 (en) 2014-12-15 2022-07-14 The University Of Queensland Differentiation of pluripotent stem cells to form renal organoids
CA2978870C (en) 2015-03-06 2024-06-25 Kyoto University Method for inducing differentiation of alveolar epithelial cells
US10563171B2 (en) 2015-06-29 2020-02-18 Kyoto University Method for inducing differentiation of pluripotent stem cells into germ cells
JP6646311B2 (en) * 2015-07-17 2020-02-14 国立大学法人京都大学 Differentiation induction method from pluripotent stem cells to mesoderm progenitor cells and blood vascular progenitor cells
DK3347456T3 (en) * 2015-09-08 2024-02-19 Us Health PROCEDURE FOR REPRODUCIBLE DIFFERENTIATION OF CLINICAL-GRADE RETINAL PIGMENT EPITHELIAL CELLS
IL257974B (en) 2015-09-11 2022-07-01 Astellas Pharma Inc Method for producing renal progenitor cells
CN105850980B (en) * 2016-04-14 2018-06-12 广州赛莱拉干细胞科技股份有限公司 The frozen stock solution and cryopreservation methods of a kind of limbal stem cell
EP3519558B1 (en) 2016-09-28 2023-08-09 Organovo, Inc. Use of engineered renal tissues in assays
CN117802033A (en) 2017-05-25 2024-04-02 国立大学法人京都大学 Method for producing mesoderm lineage primitive streak cells from pluripotent stem cells
EP3800246A4 (en) 2018-10-10 2022-04-20 National University Corporation Tottori University METHOD FOR THE PRODUCTION OF HUMAN INDUCED PLURIPOTENT STEM CELLS CONTAINING AN EXOGENOUS CHROMOSOME
JP7437766B2 (en) * 2018-10-31 2024-02-26 国立大学法人京都大学 Method for producing pluripotent stem cells that are free from mesendoderm differentiation resistance
EP3950933A4 (en) 2019-03-29 2023-01-11 Kaneka Corporation Cell population including pluripotent stem cells and production method thereof
CN110656089B (en) * 2019-10-15 2021-02-09 上海捷易生物科技有限公司 Method for directionally inducing and differentiating iPS cells into mature neurons
TW202237826A (en) 2020-11-30 2022-10-01 瑞士商克里斯珀醫療股份公司 Gene-edited natural killer cells
EP4271798A1 (en) 2020-12-30 2023-11-08 CRISPR Therapeutics AG Compositions and methods for differentiating stem cells into nk cells
CN118984871A (en) * 2022-02-15 2024-11-19 国立大学法人神户大学 Method for preparing human pluripotent stem cell-derived Leydig-like cells and human pluripotent stem cell-derived Leydig-like cell population

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
JP5098028B2 (en) 2005-12-13 2012-12-12 国立大学法人京都大学 Nuclear reprogramming factor
KR20090115142A (en) * 2007-01-30 2009-11-04 유니버시티 오브 조지아 리서치 파운데이션, 인코포레이티드 Early mesoderm cells, a stable population of mesendoderm cells that has utility for generation of endoderm and mesoderm lineages and multipotent migratory cells(mmc)

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
M. CRISTINA NOSTRO ET AL: "Wnt, Activin, and BMP Signaling Regulate Distinct Stages in the Developmental Pathway from Embryonic Stem Cells to Blood", CELL STEM CELL, vol. 2, no. 1, 1 January 2008 (2008-01-01), pages 60 - 71, XP055072737, ISSN: 1934-5909, DOI: 10.1016/j.stem.2007.10.011 *
RYUJI MORIZANE ET AL: "Differentiation of murine embryonic stem and induced pluripotent stem cells to renal lineage in vitro", BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, ACADEMIC PRESS INC. ORLANDO, FL, US, vol. 390, no. 4, 25 December 2009 (2009-12-25), pages 1334 - 1339, XP008152385, ISSN: 0006-291X, [retrieved on 20091031], DOI: 10.1016/J.BBRC.2009.10.148 *
See also references of WO2012011610A1 *
SHARON L. PAIGE ET AL: "Endogenous Wnt/[beta]-Catenin Signaling Is Required for Cardiac Differentiation in Human Embryonic Stem Cells", PLOS ONE, vol. 5, no. 6, 15 June 2010 (2010-06-15), pages e11134, XP055088752, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0011134 *
STEVEN A. JACKSON ET AL: "Differentiating Embryonic Stem Cells Pass through 'Temporal Windows' That Mark Responsiveness to Exogenous and Paracrine Mesendoderm Inducing Signals", PLOS ONE, vol. 5, no. 5, 1 January 2010 (2010-01-01), pages e10706 - e10706, XP055010718, ISSN: 1932-6203, DOI: 10.1371/journal.pone.0010706 *

Also Published As

Publication number Publication date
US9121011B2 (en) 2015-09-01
EP2596096A1 (en) 2013-05-29
JP5896360B2 (en) 2016-04-13
JP2013530680A (en) 2013-08-01
EP2596096B1 (en) 2018-01-31
WO2012011610A1 (en) 2012-01-26
US20130122536A1 (en) 2013-05-16

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