EP2245160A4 - PRESENTATION OF PROTEINS ON THE CELL SURFACE - Google Patents

PRESENTATION OF PROTEINS ON THE CELL SURFACE

Info

Publication number: EP2245160A4
Authority: EP; European Patent Office
Prior art keywords: presentation; proteins; cell surface; cell
Prior art date: 2008-01-22
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Withdrawn

Application number

EP09703905A

Other languages

German (de)

French (fr)

Other versions

EP2245160A1 (en

Inventor

Heinrich Volschenk

Mariska Lilly

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Stellenbosch University

Original Assignee

Stellenbosch University

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2008-01-22

Filing date

2009-01-21

Publication date

2011-03-30

2009-01-21 Application filed by Stellenbosch University filed Critical Stellenbosch University

2010-11-03 Publication of EP2245160A1 publication Critical patent/EP2245160A1/en

2011-03-30 Publication of EP2245160A4 publication Critical patent/EP2245160A4/en

Status Withdrawn legal-status Critical Current

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/62—DNA sequences coding for fusion proteins
- C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
- C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor

Landscapes

Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Life Sciences & Earth Sciences (AREA)
Engineering & Computer Science (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Biomedical Technology (AREA)
Bioinformatics & Cheminformatics (AREA)
Biotechnology (AREA)
General Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
General Health & Medical Sciences (AREA)
Biophysics (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Microbiology (AREA)
Plant Pathology (AREA)
Mycology (AREA)
Gastroenterology & Hepatology (AREA)
Medicinal Chemistry (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

EP09703905A 2008-01-22 2009-01-21 PRESENTATION OF PROTEINS ON THE CELL SURFACE Withdrawn EP2245160A4 (en)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
ZA200800623		2008-01-22
PCT/IB2009/000086 WO2009093118A1 (en)	2008-01-22	2009-01-21	Cell surface display of proteins

Publications (2)

Publication Number	Publication Date
EP2245160A1 EP2245160A1 (en)	2010-11-03
EP2245160A4 true EP2245160A4 (en)	2011-03-30

Family

ID=40900797

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
EP09703905A Withdrawn EP2245160A4 (en)	2008-01-22	2009-01-21	PRESENTATION OF PROTEINS ON THE CELL SURFACE

Country Status (2)

Country	Link
EP (1)	EP2245160A4 (en)
WO (1)	WO2009093118A1 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO2010057064A2 (en)	2008-11-15	2010-05-20	The Regents Of The University Of California	Engineering of yeast for cellulosic ethanol production
US9315833B2 (en)	2009-02-20	2016-04-19	Lallemand Hungary Liquidity Management Llc	Yeast cells expressing an exogenous cellulosome and methods of using the same
DE102010056289A1 (en)	2010-12-24	2012-06-28	Geneart Ag	Process for the preparation of reading frame correct fragment libraries
US8765425B2 (en)	2011-03-23	2014-07-01	Butamax Advanced Biofuels Llc	In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US8759044B2 (en)	2011-03-23	2014-06-24	Butamax Advanced Biofuels Llc	In situ expression of lipase for enzymatic production of alcohol esters during fermentation
US20160002645A1 (en) *	2012-11-16	2016-01-07	The Regents Of The University Of California	Recombinant bacillus subtilis that can grow on plant biomass
WO2016007886A1 (en) *	2014-07-11	2016-01-14	Northwestern University	Yeast-based biosensor
CN108676811B (en) *	2018-05-28	2021-07-13	郝志敏	Gene traceless editing vector and application thereof in organism gene editing
CN111961690A (en) *	2020-08-19	2020-11-20	首都师范大学	Method for producing ethanol by batch-type supplementary material simultaneous saccharification and fermentation of pennisetum alopecuroides through steam explosion
CN118726441A (en) *	2024-06-20	2024-10-01	广州大学	A ganoderma lucidum surface display system and its preparation method and application

Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1999046362A1 (en) *	1998-03-10	1999-09-16	Iogen Corporation	Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
IL111415A0 (en) *	1994-10-27	1994-12-29	Yeda Res & Dev	Modified cellulose-binding domain (cbd) proteins and use thereof

2009
- 2009-01-21 WO PCT/IB2009/000086 patent/WO2009093118A1/en active Application Filing
- 2009-01-21 EP EP09703905A patent/EP2245160A4/en not_active Withdrawn

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
WO1999046362A1 (en) *	1998-03-10	1999-09-16	Iogen Corporation	Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase

Non-Patent Citations (6)

* Cited by examiner, † Cited by third party
Title
BAYER ET AL: "The potential of cellulases and cellulosomes for cellulosic waste management", CURRENT OPINION IN BIOTECHNOLOGY, LONDON, GB, vol. 18, no. 3, 8 June 2007 (2007-06-08), pages 237 - 245, XP022110187, ISSN: 0958-1669, DOI: 10.1016/J.COPBIO.2007.04.004 *
FIEROBE H P ET AL: "Design and production of active cellulosome chimeras. Selective incorporation of dockerin-containing enzymes into defined functional complexes", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 276, no. 24, 15 June 2001 (2001-06-15), pages 21257 - 21261, XP002498644, ISSN: 0021-9258, [retrieved on 20010404], DOI: 10.1074/JBC.M102082200 *
FIEROBE H-P ET AL: "Degradation of Cellulose Substrates by Cellulosome Chimeras - SUBSTRATE TARGETING VERSUS PROXIMITY OF ENZYME COMPONENTS", JOURNAL OF BIOLOGICAL CHEMISTRY, AMERICAN SOCIETY FOR BIOCHEMISTRY AND MOLECULAR BIOLOGY, INC, US, vol. 277, no. 51, 20 December 2002 (2002-12-20), pages 49621 - 49630, XP002613753, ISSN: 0021-9258, [retrieved on 20021022], DOI: 10.1074/JBC.M207672200 *
LILLY MARISKA ET AL: "Heterologous expression of a Clostridium minicellulosome in Saccharomyces cerevisiae", FEMS YEAST RESEARCH, vol. 9, no. 8, December 2009 (2009-12-01), pages 1236 - 1249, XP002622026 *
See also references of WO2009093118A1 *
VAN DER VAART J MARCEL ET AL: "Comparison of cell wall proteins of Saccharomyces cerevisiae as anchors for cell surface expression of heterologous proteins", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, AMERICAN SOCIETY FOR MICROBIOLOGY, US, vol. 63, no. 2, 1 February 1997 (1997-02-01), pages 615 - 620, XP009130380, ISSN: 0099-2240 *

Also Published As

Publication number	Publication date
EP2245160A1 (en)	2010-11-03
WO2009093118A9 (en)	2009-11-05
WO2009093118A1 (en)	2009-07-30

Publication	Publication Date	Title
EP2245160A4 (en)	2011-03-30	PRESENTATION OF PROTEINS ON THE CELL SURFACE
EP2030015A4 (en)	2009-11-11	REMODELING THE SURFACE OF PROTEINS
EP2005504A4 (en)	2012-04-04	ELECTROLYTE
EP2211903A4 (en)	2011-07-06	ANTI-CLL-1 ANTIBODIES
DK3118221T3 (en)	2019-09-23	PROTEINS
EP2220247A4 (en)	2011-10-26	ANTIBODIES DIRECTED AGAINST LRP6
EP2215246A4 (en)	2011-08-10	PROTEIN STRUCTURES
EP2212342A4 (en)	2010-11-17	Electrochemical assay for the detection of enzymes
EP2172483A4 (en)	2010-08-04	ANTI-MUC17 ANTIBODIES
DK1979225T3 (en)	2014-11-24	mooring
EP2212431A4 (en)	2011-05-11	ANTI-IREM-1 ANTIBODIES
DK2027150T3 (en)	2012-08-20	ALLO-LIMITED PEPTID-SPECIFIC T CELLS
DK2146893T3 (en)	2012-09-10	mooring
FI20060905A0 (en)	2006-10-12	wayfinding
NO20076008L (en)	2009-05-22	The holder
NO20082399L (en)	2008-07-18	Rudder branching device
EP2192402A4 (en)	2010-09-01	ENZYME ELECTRODE
EP1981964A4 (en)	2010-01-13	SECA2 MUTANTS MYCOBACTERIANS
DE112006002477A5 (en)	2008-06-26	electrolyte
FI20055692A0 (en)	2005-12-22	New enzymes
DK2238159T3 (en)	2013-10-21	Hitherto unknown protein
FI7890U1 (en)	2008-05-30	The support member
BRPI0906053A2 (en)	2015-06-30	"artificial enzyme"
FI20085306A0 (en)	2008-04-11	The bridge structure
FI20065035A0 (en)	2006-01-19	Essentially pure enzyme

Legal Events

Date	Code	Title	Description
2010-10-01	PUAI	Public reference made under article 153(3) epc to a published international application that has entered the european phase	Free format text: ORIGINAL CODE: 0009012
2010-11-03	17P	Request for examination filed	Effective date: 20100818
2010-11-03	AK	Designated contracting states	Kind code of ref document: A1 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MK MT NL NO PL PT RO SE SI SK TR
2010-11-03	AX	Request for extension of the european patent	Extension state: AL BA RS
2011-03-30	A4	Supplementary search report drawn up and despatched	Effective date: 20110225
2011-04-27	DAX	Request for extension of the european patent (deleted)
2011-12-30	STAA	Information on the status of an ep patent application or granted ep patent	Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN
2012-02-01	18D	Application deemed to be withdrawn	Effective date: 20110201