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EP2195447A4 - Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate - Google Patents

Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate

Info

Publication number
EP2195447A4
EP2195447A4 EP08800381A EP08800381A EP2195447A4 EP 2195447 A4 EP2195447 A4 EP 2195447A4 EP 08800381 A EP08800381 A EP 08800381A EP 08800381 A EP08800381 A EP 08800381A EP 2195447 A4 EP2195447 A4 EP 2195447A4
Authority
EP
European Patent Office
Prior art keywords
nucleotide triphosphate
gamma phosphate
label conjugated
electroactive label
electroactive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP08800381A
Other languages
German (de)
French (fr)
Other versions
EP2195447A1 (en
Inventor
Heinz-Bernhard Kraatz
Kagan Kerman
Haifeng Song
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of EP2195447A1 publication Critical patent/EP2195447A1/en
Publication of EP2195447A4 publication Critical patent/EP2195447A4/en
Withdrawn legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/485Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/06Pyrimidine radicals
    • C07H19/10Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H19/00Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
    • C07H19/02Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
    • C07H19/04Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
    • C07H19/16Purine radicals
    • C07H19/20Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
    • G01N33/581Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2458/00Labels used in chemical analysis of biological material
    • G01N2458/30Electrochemically active labels

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
EP08800381A 2007-09-27 2008-09-26 Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate Withdrawn EP2195447A4 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US96038807P 2007-09-27 2007-09-27
PCT/CA2008/001690 WO2009039639A1 (en) 2007-09-27 2008-09-26 Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate

Publications (2)

Publication Number Publication Date
EP2195447A1 EP2195447A1 (en) 2010-06-16
EP2195447A4 true EP2195447A4 (en) 2011-03-30

Family

ID=40510705

Family Applications (1)

Application Number Title Priority Date Filing Date
EP08800381A Withdrawn EP2195447A4 (en) 2007-09-27 2008-09-26 Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate

Country Status (4)

Country Link
EP (1) EP2195447A4 (en)
CN (1) CN102016064A (en)
CA (1) CA2700945A1 (en)
WO (1) WO2009039639A1 (en)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010144150A2 (en) 2009-06-12 2010-12-16 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
CN105548301B (en) * 2016-01-28 2018-07-31 中南大学 A kind of method and the active method of kinases inhibitor measuring protein kinase activity
CN109030583B (en) * 2018-08-28 2023-09-15 长沙理工大学 Detection method and sensor of L-cysteine based on 2-mercaptobenzimidazole
CN111693707A (en) * 2020-07-16 2020-09-22 首都医科大学附属北京朝阳医院 Application of ROCK kinase activity in auxiliary diagnosis of vasculitis patients
CN114235922A (en) * 2020-09-09 2022-03-25 中国科学院大连化学物理研究所 A method for real-time monitoring of phosphorylation using functional nanochannels
CN113430248B (en) * 2021-06-28 2022-11-11 中国人民解放军军事科学院军事医学研究院 Non-isotope screening method of novel p53 protein kinase and application thereof

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1194593A2 (en) * 1999-07-20 2002-04-10 Clinical Micro Sensors, Inc. Amplification of nucleic acids with electronic detection
EP1616034A4 (en) * 2003-04-01 2008-12-03 Activx Biosciences Inc Acyl- phosphate and phosphonate probes and methods of their synthesis and use in proteomic analysis

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NAVARRO A-E ET AL: "Supported synthesis of ferrocene modified oligonucleotides as new electroactive DNA probes", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON, ELSEVIER SCIENCE, GB, vol. 14, no. 10, 17 May 2004 (2004-05-17), pages 2439 - 2441, XP004841216, ISSN: 0960-894X, DOI: 10.1016/J.BMCL.2004.03.016 *
See also references of WO2009039639A1 *

Also Published As

Publication number Publication date
CN102016064A (en) 2011-04-13
EP2195447A1 (en) 2010-06-16
WO2009039639A8 (en) 2009-05-07
CA2700945A1 (en) 2009-04-02
WO2009039639A1 (en) 2009-04-02

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Legal Events

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PUAI Public reference made under article 153(3) epc to a published international application that has entered the european phase

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17P Request for examination filed

Effective date: 20100330

AK Designated contracting states

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Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR

AX Request for extension of the european patent

Extension state: AL BA MK RS

DAX Request for extension of the european patent (deleted)
A4 Supplementary search report drawn up and despatched

Effective date: 20110225

17Q First examination report despatched

Effective date: 20140910

STAA Information on the status of an ep patent application or granted ep patent

Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN

18D Application deemed to be withdrawn

Effective date: 20150121