EP2195447A4 - Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate - Google Patents
Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphateInfo
- Publication number
- EP2195447A4 EP2195447A4 EP08800381A EP08800381A EP2195447A4 EP 2195447 A4 EP2195447 A4 EP 2195447A4 EP 08800381 A EP08800381 A EP 08800381A EP 08800381 A EP08800381 A EP 08800381A EP 2195447 A4 EP2195447 A4 EP 2195447A4
- Authority
- EP
- European Patent Office
- Prior art keywords
- nucleotide triphosphate
- gamma phosphate
- label conjugated
- electroactive label
- electroactive
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
- 229910019142 PO4 Inorganic materials 0.000 title 1
- 239000002773 nucleotide Substances 0.000 title 1
- 125000003729 nucleotide group Chemical group 0.000 title 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 title 1
- 239000010452 phosphate Substances 0.000 title 1
- 235000011178 triphosphate Nutrition 0.000 title 1
- 239000001226 triphosphate Substances 0.000 title 1
- UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/485—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving kinase
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/06—Pyrimidine radicals
- C07H19/10—Pyrimidine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H19/00—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof
- C07H19/02—Compounds containing a hetero ring sharing one ring hetero atom with a saccharide radical; Nucleosides; Mononucleotides; Anhydro-derivatives thereof sharing nitrogen
- C07H19/04—Heterocyclic radicals containing only nitrogen atoms as ring hetero atom
- C07H19/16—Purine radicals
- C07H19/20—Purine radicals with the saccharide radical esterified by phosphoric or polyphosphoric acids
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/581—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with enzyme label (including co-enzymes, co-factors, enzyme inhibitors or substrates)
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2458/00—Labels used in chemical analysis of biological material
- G01N2458/30—Electrochemically active labels
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Wood Science & Technology (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Other Investigation Or Analysis Of Materials By Electrical Means (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US96038807P | 2007-09-27 | 2007-09-27 | |
| PCT/CA2008/001690 WO2009039639A1 (en) | 2007-09-27 | 2008-09-26 | Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| EP2195447A1 EP2195447A1 (en) | 2010-06-16 |
| EP2195447A4 true EP2195447A4 (en) | 2011-03-30 |
Family
ID=40510705
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| EP08800381A Withdrawn EP2195447A4 (en) | 2007-09-27 | 2008-09-26 | Nucleotide triphosphate with an electroactive label conjugated to the gamma phosphate |
Country Status (4)
| Country | Link |
|---|---|
| EP (1) | EP2195447A4 (en) |
| CN (1) | CN102016064A (en) |
| CA (1) | CA2700945A1 (en) |
| WO (1) | WO2009039639A1 (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010144150A2 (en) | 2009-06-12 | 2010-12-16 | Pacific Biosciences Of California, Inc. | Real-time analytical methods and systems |
| CN105548301B (en) * | 2016-01-28 | 2018-07-31 | 中南大学 | A kind of method and the active method of kinases inhibitor measuring protein kinase activity |
| CN109030583B (en) * | 2018-08-28 | 2023-09-15 | 长沙理工大学 | Detection method and sensor of L-cysteine based on 2-mercaptobenzimidazole |
| CN111693707A (en) * | 2020-07-16 | 2020-09-22 | 首都医科大学附属北京朝阳医院 | Application of ROCK kinase activity in auxiliary diagnosis of vasculitis patients |
| CN114235922A (en) * | 2020-09-09 | 2022-03-25 | 中国科学院大连化学物理研究所 | A method for real-time monitoring of phosphorylation using functional nanochannels |
| CN113430248B (en) * | 2021-06-28 | 2022-11-11 | 中国人民解放军军事科学院军事医学研究院 | Non-isotope screening method of novel p53 protein kinase and application thereof |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1194593A2 (en) * | 1999-07-20 | 2002-04-10 | Clinical Micro Sensors, Inc. | Amplification of nucleic acids with electronic detection |
| EP1616034A4 (en) * | 2003-04-01 | 2008-12-03 | Activx Biosciences Inc | Acyl- phosphate and phosphonate probes and methods of their synthesis and use in proteomic analysis |
-
2008
- 2008-09-26 EP EP08800381A patent/EP2195447A4/en not_active Withdrawn
- 2008-09-26 CA CA2700945A patent/CA2700945A1/en not_active Abandoned
- 2008-09-26 CN CN2008801094672A patent/CN102016064A/en active Pending
- 2008-09-26 WO PCT/CA2008/001690 patent/WO2009039639A1/en not_active Ceased
Non-Patent Citations (2)
| Title |
|---|
| NAVARRO A-E ET AL: "Supported synthesis of ferrocene modified oligonucleotides as new electroactive DNA probes", BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, PERGAMON, ELSEVIER SCIENCE, GB, vol. 14, no. 10, 17 May 2004 (2004-05-17), pages 2439 - 2441, XP004841216, ISSN: 0960-894X, DOI: 10.1016/J.BMCL.2004.03.016 * |
| See also references of WO2009039639A1 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102016064A (en) | 2011-04-13 |
| EP2195447A1 (en) | 2010-06-16 |
| WO2009039639A8 (en) | 2009-05-07 |
| CA2700945A1 (en) | 2009-04-02 |
| WO2009039639A1 (en) | 2009-04-02 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUAI | Public reference made under article 153(3) epc to a published international application that has entered the european phase |
Free format text: ORIGINAL CODE: 0009012 |
|
| 17P | Request for examination filed |
Effective date: 20100330 |
|
| AK | Designated contracting states |
Kind code of ref document: A1 Designated state(s): AT BE BG CH CY CZ DE DK EE ES FI FR GB GR HR HU IE IS IT LI LT LU LV MC MT NL NO PL PT RO SE SI SK TR |
|
| AX | Request for extension of the european patent |
Extension state: AL BA MK RS |
|
| DAX | Request for extension of the european patent (deleted) | ||
| A4 | Supplementary search report drawn up and despatched |
Effective date: 20110225 |
|
| 17Q | First examination report despatched |
Effective date: 20140910 |
|
| STAA | Information on the status of an ep patent application or granted ep patent |
Free format text: STATUS: THE APPLICATION IS DEEMED TO BE WITHDRAWN |
|
| 18D | Application deemed to be withdrawn |
Effective date: 20150121 |