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EP2162537A4 - USE OF GENETICALLY MODIFIED ORGANISMS FOR GENERATING BIOMASS DECOMPOSITION ENZYMES - Google Patents

USE OF GENETICALLY MODIFIED ORGANISMS FOR GENERATING BIOMASS DECOMPOSITION ENZYMES

Info

Publication number
EP2162537A4
EP2162537A4 EP08767983A EP08767983A EP2162537A4 EP 2162537 A4 EP2162537 A4 EP 2162537A4 EP 08767983 A EP08767983 A EP 08767983A EP 08767983 A EP08767983 A EP 08767983A EP 2162537 A4 EP2162537 A4 EP 2162537A4
Authority
EP
European Patent Office
Prior art keywords
genetically modified
enzymes
modified organisms
biomass decomposition
methods
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP08767983A
Other languages
German (de)
French (fr)
Other versions
EP2162537B1 (en
EP2162537A2 (en
Inventor
Stephen Mayfield
Bryan O'neill
Michael Mendez
Yan Poon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Scripps Research Institute
Sapphire Energy Inc
Original Assignee
Scripps Research Institute
Sapphire Energy Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Institute, Sapphire Energy Inc filed Critical Scripps Research Institute
Priority to EP11184458.5A priority Critical patent/EP2463370B1/en
Publication of EP2162537A2 publication Critical patent/EP2162537A2/en
Publication of EP2162537A4 publication Critical patent/EP2162537A4/en
Application granted granted Critical
Publication of EP2162537B1 publication Critical patent/EP2162537B1/en
Not-in-force legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8214—Plastid transformation
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Immunology (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Processing Of Solid Wastes (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention provides a method and compositions for high throughput screening of genetically modified photosynthetic organisms for plasmic state. The present invention provides methods of producing one or more proteins, including biomass degrading enzymes in a plant. Also provided are the methods of producing biomass degradation pathways in alga cells, particularly in the chloroplast. Single enzymes or multiple enzymes may be produced by the methods disclosed. The methods disclosed herein allow for the production of biofuel, including ethanol.
EP08767983A 2007-06-01 2008-05-30 Use of genetically modified organisms to generate biomass degrading enzymes Not-in-force EP2162537B1 (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP11184458.5A EP2463370B1 (en) 2007-06-01 2008-05-30 Use of genetically modified organisms to generate biomass degrading enzymes

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US94145207P 2007-06-01 2007-06-01
US7038408P 2008-03-20 2008-03-20
US7043708P 2008-03-20 2008-03-20
PCT/US2008/006879 WO2008150463A2 (en) 2007-06-01 2008-05-30 Use of genetically modified organisms to generate biomass degrading enzymes

Related Child Applications (1)

Application Number Title Priority Date Filing Date
EP11184458.5 Division-Into 2011-10-10

Publications (3)

Publication Number Publication Date
EP2162537A2 EP2162537A2 (en) 2010-03-17
EP2162537A4 true EP2162537A4 (en) 2010-07-14
EP2162537B1 EP2162537B1 (en) 2012-01-04

Family

ID=40094332

Family Applications (3)

Application Number Title Priority Date Filing Date
EP08767983A Not-in-force EP2162537B1 (en) 2007-06-01 2008-05-30 Use of genetically modified organisms to generate biomass degrading enzymes
EP20080767981 Ceased EP2171085A4 (en) 2007-06-01 2008-05-30 HIGH PERFORMANCE SCREENING OF GENETICALLY MODIFIED PHOTOSYNTHESIS ORGANISMS
EP11184458.5A Not-in-force EP2463370B1 (en) 2007-06-01 2008-05-30 Use of genetically modified organisms to generate biomass degrading enzymes

Family Applications After (2)

Application Number Title Priority Date Filing Date
EP20080767981 Ceased EP2171085A4 (en) 2007-06-01 2008-05-30 HIGH PERFORMANCE SCREENING OF GENETICALLY MODIFIED PHOTOSYNTHESIS ORGANISMS
EP11184458.5A Not-in-force EP2463370B1 (en) 2007-06-01 2008-05-30 Use of genetically modified organisms to generate biomass degrading enzymes

Country Status (15)

Country Link
US (4) US8143039B2 (en)
EP (3) EP2162537B1 (en)
JP (2) JP2010528609A (en)
KR (2) KR20130020842A (en)
CN (2) CN101835901B (en)
AT (1) ATE540112T1 (en)
AU (2) AU2008260555B2 (en)
BR (1) BRPI0812042A2 (en)
CA (2) CA2857507C (en)
DK (1) DK2162537T3 (en)
IL (2) IL202303A (en)
MX (2) MX2009012840A (en)
NZ (2) NZ581472A (en)
SG (1) SG178735A1 (en)
WO (2) WO2008150461A2 (en)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ581472A (en) * 2007-06-01 2012-02-24 Sapphire Energy Inc High throughput screening of genetically modified photosynthetic organisms
US8314222B2 (en) 2007-10-05 2012-11-20 Sapphire Energy, Inc. System for capturing and modifying large pieces of genomic DNA and constructing organisms with chloroplasts
WO2009045550A2 (en) * 2007-10-05 2009-04-09 Sapphire Energy System for capturing and modifying large pieces of genomic dna and constructing organisms with synthetic chloroplasts
EP2220125A4 (en) * 2007-11-13 2010-12-29 Sapphire Energy Inc Production of fc-fusion polypeptides in eukaryotic algae
JP5777884B2 (en) 2008-01-03 2015-09-09 プロテロ インコーポレイテッド Transgenic photosynthetic microorganisms and photobioreactors
WO2010051489A1 (en) * 2008-10-31 2010-05-06 Sapphire Energy, Inc. Animal feedstock comprising genetically modified algae
FI122957B (en) * 2009-06-24 2012-09-14 Neste Oil Oyj A process for producing fat
CN101892159B (en) * 2010-01-15 2012-03-28 新奥科技发展有限公司 A strain of Chlamydomonas algae and its application
US20120322106A1 (en) * 2010-02-18 2012-12-20 Bobban Subhadra Production of Cellulases and Hemicellulases in Algal Biofuel Feedstocks
CN103124499B (en) * 2010-05-28 2016-09-28 泰拉瑞亚控股公司 Comprise the food compositions of tailor-made oil
KR101964886B1 (en) 2010-11-03 2019-04-03 테라비아 홀딩스 인코포레이티드 Microbial oils with lowered pour points, dielectric fluids produced therefrom, and releated methods
US9315838B2 (en) 2012-11-07 2016-04-19 Board Of Trustees Of Michigan State University Method to increase algal biomass and enhance its quality for the production of fuel
WO2014130357A1 (en) 2013-02-19 2014-08-28 Heliae Development, Llc Bioreactor array and methods of combinatorial testing
DE112014003580B4 (en) * 2013-08-02 2018-04-26 Euglena Co., Ltd. Endo-β-1,3-glucanase, polynucleotide, recombinant vector, transformant, production method for endo-β-1,3-glucanase, enzyme production and production of reduced molecular weight paramylon
EP3544994B1 (en) 2016-11-27 2024-09-25 Triton Algae Innovations, Inc. Method of purification of recombinant osteopontin from microalgae
CN112646830A (en) * 2019-10-10 2021-04-13 天津科技大学 Universal plasmid and construction method thereof and novel method for synechocystis to express exogenous gene
CN110684704A (en) * 2019-10-11 2020-01-14 天津科技大学 Gene engineering strain of synechocystis PCC6803 for producing cellulase and construction method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1574580A2 (en) * 1996-09-12 2005-09-14 Syngenta Participations AG Transgenic plants expressing cellulolytic enzymes
WO2007005646A2 (en) * 2005-07-01 2007-01-11 The University Of Florida Research Foundation, Inc. Recombinant host cells and media for ethanol production

Family Cites Families (74)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IL57712A (en) * 1979-07-03 1984-02-29 Yissum Res Dev Co Cultivation of halophilic algae of the dunaliella species for the production of fuel-like product
JPS5939000B2 (en) 1979-07-04 1984-09-20 積水化成品工業株式会社 fuel composition
AT387790B (en) 1983-06-09 1989-03-10 Vogelbusch Gmbh METHOD FOR DIGESTING STARCH FOR THE PRODUCTION OF SUGARIZED MASH
US5559031A (en) 1987-08-12 1996-09-24 Technipetrol S.P.A. Apparatus for the continuous production of ethanol from cereals
US5200215A (en) 1988-04-20 1993-04-06 Nabisco, Inc. Enzyme treated low moisture content comestible products
US4795479A (en) * 1988-05-02 1989-01-03 R. T. Vanderbilt Company, Inc. Fuel compositions containing terpene derivatives of 2,5-dimercapto-1,3,4-thiadiazole
US6680426B2 (en) * 1991-01-07 2004-01-20 Auburn University Genetic engineering of plant chloroplasts
US5932479A (en) * 1988-09-26 1999-08-03 Auburn University Genetic engineering of plant chloroplasts
US7129391B1 (en) * 1988-09-26 2006-10-31 Auburn University Universal chloroplast integration and expression vectors, transformed plants and products thereof
US5693507A (en) 1988-09-26 1997-12-02 Auburn University Genetic engineering of plant chloroplasts
US5451513A (en) 1990-05-01 1995-09-19 The State University of New Jersey Rutgers Method for stably transforming plastids of multicellular plants
US5877402A (en) * 1990-05-01 1999-03-02 Rutgers, The State University Of New Jersey DNA constructs and methods for stably transforming plastids of multicellular plants and expressing recombinant proteins therein
IE20040144A1 (en) 1990-07-24 2004-05-19 Dsm Nv Cloning and expression of xylanase genes from fungal origin
GB9304200D0 (en) 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
JP2911310B2 (en) 1992-07-07 1999-06-23 オルガノ株式会社 Method and apparatus for producing glucose
US5661017A (en) 1993-09-14 1997-08-26 Dunahay; Terri Goodman Method to transform algae, materials therefor, and products produced thereby
CA2118071C (en) * 1993-10-28 2004-09-14 Rodney B. Croteau Dna encoding limonene synthase from mentha spicata
US5576198A (en) 1993-12-14 1996-11-19 Calgene, Inc. Controlled expression of transgenic constructs in plant plastids
US5958761A (en) 1994-01-12 1999-09-28 Yeda Research And Developement Co. Ltd. Bioreactor and system for improved productivity of photosynthetic algae
US5545817A (en) 1994-03-11 1996-08-13 Calgene, Inc. Enhanced expression in a plant plastid
US5545818A (en) * 1994-03-11 1996-08-13 Calgene Inc. Expression of Bacillus thuringiensis cry proteins in plant plastids
US5607486A (en) * 1994-05-04 1997-03-04 Wilkins, Jr.; Joe S. Engine fuels
USRE37629E1 (en) * 1994-05-04 2002-04-09 Joe S. Wilkins, Jr. Engine fuels
US5767373A (en) 1994-06-16 1998-06-16 Novartis Finance Corporation Manipulation of protoporphyrinogen oxidase enzyme activity in eukaryotic organisms
AU2568697A (en) 1996-04-12 1997-11-07 Novo Nordisk A/S An enzyme with beta-1,3-glucanase activity
US5824774A (en) 1996-04-12 1998-10-20 Board Of Trustees Of The University Of Kentucky Chimeric isoprenoid synthases and uses thereof
JP2000503858A (en) * 1996-06-14 2000-04-04 ラトガーズ・ザ・ステイト・ユニバーシティ・オブ・ニュージャージー Plastid marker genes that can be selected based on editing
US5776689A (en) 1996-07-19 1998-07-07 The Regents Of The University Of California Protein recruitment system
ES2242234T3 (en) * 1996-09-12 2005-11-01 Syngenta Participations Ag TRANSGENIC PLANTS THAT EXPRESS CELLULOLYTIC ENZYMES.
JP2002513283A (en) 1997-01-17 2002-05-08 ザ スクリップス リサーチ インスティテュート RNA binding proteins and binding sites useful for expression of recombinant molecules
JP3562280B2 (en) 1997-12-16 2004-09-08 トヨタ自動車株式会社 Geranyl diphosphate synthase gene
JPH11226351A (en) 1998-02-12 1999-08-24 Spirulina Kenkyusho:Kk Production of cleaned air and apparatus for cleaning air
CN1292824A (en) 1998-03-11 2001-04-25 诺瓦提斯公司 Expression of trehalose biosynthetic genes in plants
WO2001072959A2 (en) 2000-03-01 2001-10-04 Auburn University Plastid transformation vectors for expressing human proteins in plants
US6271444B1 (en) 1998-07-10 2001-08-07 Calgene Llc Enhancer elements for increased translation in plant plastids
PT1097211E (en) 1998-07-10 2007-07-12 Calgene Llc Expression of eukaryotic peptides in plant plastids
EP1102528B1 (en) 1998-08-03 2008-11-19 Rutgers University Translation control elements for high-level protein expression in the plastids of higher plants and methods of use thereof
AR022383A1 (en) * 1998-09-18 2002-09-04 Univ Kentucky Res Found SYNTHESES
US6515206B1 (en) 1998-12-23 2003-02-04 Calgene Llc Plastid transformation of Brassica
CA2387734A1 (en) 1999-10-18 2001-04-26 Washington State University Research Foundation Geranyl diphosphate synthase large subunit, and methods of use
WO2001042441A2 (en) * 1999-12-08 2001-06-14 International Centre For Genetic Engineering And Biotechnology Plastid transformation
US20030041353A1 (en) * 2001-04-18 2003-02-27 Henry Daniell Mutiple gene expression for engineering novel pathways and hyperexpression of foreign proteins in plants
EP1268832A4 (en) 2000-02-29 2003-04-02 Univ Auburn Production of antibodies in transgenic plastids
EP1360300A2 (en) * 2000-07-31 2003-11-12 Frederick M. Hahn Manipulation of genes of the mevalonate and isoprenoid pathways to create novel traits in transgenic organisms
AU9635901A (en) 2000-09-29 2002-04-08 Cargill Inc Isoprenoid production
EP1231273A1 (en) 2001-02-12 2002-08-14 Plant Research International B.V. Terpene synthase/cyclase and olefin synthase and uses thereof
US20030144879A1 (en) * 2001-02-28 2003-07-31 Shinji Hata System for providing medical service
CA2440278C (en) 2001-03-09 2017-02-28 University Of Kentucky Research Foundation Cytochrome p450s and uses thereof
US7172886B2 (en) 2001-12-06 2007-02-06 The Regents Of The University Of California Biosynthesis of isopentenyl pyrophosphate
US20030162273A1 (en) 2002-02-04 2003-08-28 Anastasios Melis Modulation of sulfate permease for photosynthetic hydrogen production
DE60320382D1 (en) 2002-02-28 2008-05-29 Takara Bio Inc PROMOTER and INTRON FROM ALGAE
JP2005537784A (en) 2002-04-23 2005-12-15 ザ スクリップス リサーチ インスティテュート Expression of polypeptides in chloroplasts and compositions and methods for expressing the polypeptides
WO2003095651A1 (en) * 2002-05-10 2003-11-20 Kyowa Hakko Kogyo Co., Ltd. Process for producing mevalonic acid
EA009596B1 (en) * 2002-05-13 2008-02-28 Гринфьюел Текнолоджиз Корпорейшн Photobioreactor and process for biomass production and mitigation of pollutants in flue gases
US20050239182A1 (en) * 2002-05-13 2005-10-27 Isaac Berzin Synthetic and biologically-derived products produced using biomass produced by photobioreactors configured for mitigation of pollutants in flue gases
WO2004011667A2 (en) 2002-07-31 2004-02-05 Washington State University Research Foundation Geranyl diphosphate synthase molecules, and nucleic acid molecules encoding same
US7279018B2 (en) * 2002-09-06 2007-10-09 Fortum Oyj Fuel composition for a diesel engine
EP1554387B1 (en) 2002-10-15 2012-01-25 Syngenta Participations AG Plastid transformation
US20040074760A1 (en) 2002-10-17 2004-04-22 Carnegie Mellon University Production of biofuels
US6872516B2 (en) 2003-04-16 2005-03-29 Advanced Breath Diagnostics, Llc Methods of producing carbon-13 labeled biomass
US20050154669A1 (en) 2004-01-08 2005-07-14 Foy Streetman Carbon credit marketing system
CA2574593C (en) * 2004-07-27 2016-07-05 The Regents Of The University Of California Genetically modified host cells and use of same for producing isoprenoid compounds
WO2006093877A1 (en) * 2005-02-28 2006-09-08 Michigan State University Improved biodiesel additive and method of preparation thereof
CN101163795B (en) 2005-04-19 2014-04-23 肯塔基大学研究机构 Transformed plants accumulating terpenes
AP3827A (en) 2005-07-05 2016-09-30 Univ California Polynucleotides encoding isoprenoid modifying enzymes and methods of use thereof
CN101341243A (en) * 2005-08-25 2009-01-07 索利克斯生物燃料公司 Method, apparatus and system for biodiesel production from algae
US7735235B2 (en) 2005-09-29 2010-06-15 Ez-Snap Products Inc. Marking and fastening device and method of using same
EP2004817A2 (en) * 2006-03-30 2008-12-24 Novozymes, Inc. Polypeptides having endoglucanase activity and polynucleotides encoding same
WO2007133558A2 (en) 2006-05-09 2007-11-22 The Scripps Research Institute Robust expression of a bioactive mammalian protein in chlamydomonas chloroplast
US7947478B2 (en) * 2006-06-29 2011-05-24 The Regents Of The University Of California Short chain volatile hydrocarbon production using genetically engineered microalgae, cyanobacteria or bacteria
US7846222B2 (en) 2006-10-10 2010-12-07 Amyris Biotechnologies, Inc. Fuel compositions comprising farnesane and farnesane derivatives and method of making and using same
RU2461411C2 (en) * 2007-04-12 2012-09-20 СЕФКО, ЭлЭлСи Method and device for catching carbon and removing multiple contaminants in gas flows from hydrocarbon fuel sources and removing multiple byproducts
NZ581472A (en) * 2007-06-01 2012-02-24 Sapphire Energy Inc High throughput screening of genetically modified photosynthetic organisms
BRPI0811967B1 (en) 2007-06-01 2023-01-17 Terravia Holdings, Inc MICROALGAE CHLORELLA OR PROTOTHECA AND METHOD FOR THE PRODUCTION OF MICROBIAL LIPIDS

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1574580A2 (en) * 1996-09-12 2005-09-14 Syngenta Participations AG Transgenic plants expressing cellulolytic enzymes
WO2007005646A2 (en) * 2005-07-01 2007-01-11 The University Of Florida Research Foundation, Inc. Recombinant host cells and media for ethanol production

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
YU REIKO ET AL: "Production of eicosapentaenoic acid by a recombinant marine cyanobacterium, Synechococcus sp", LIPIDS, SPRINGER, US LNKD- DOI:10.1007/S11745-000-0619-6, vol. 35, no. 10, 1 October 2000 (2000-10-01), pages 1061 - 1064, XP009130290, ISSN: 0024-4201 *

Also Published As

Publication number Publication date
US8143039B2 (en) 2012-03-27
EP2162537B1 (en) 2012-01-04
IL202349A (en) 2013-04-30
MX2009012840A (en) 2010-02-24
AU2008260557B2 (en) 2012-08-02
KR20100031700A (en) 2010-03-24
IL202303A (en) 2014-12-31
EP2463370B1 (en) 2013-07-31
US20090246766A1 (en) 2009-10-01
NZ581473A (en) 2012-02-24
KR20130020842A (en) 2013-02-28
MX2009012839A (en) 2010-02-24
AU2008260555A2 (en) 2010-03-04
CA2692893C (en) 2015-06-23
WO2008150461A4 (en) 2009-04-09
WO2008150461A3 (en) 2009-02-05
AU2008260555B2 (en) 2012-07-26
JP2010528609A (en) 2010-08-26
US8268553B2 (en) 2012-09-18
AU2008260555A1 (en) 2008-12-11
IL202303A0 (en) 2011-08-01
EP2162537A2 (en) 2010-03-17
WO2008150463A8 (en) 2009-08-06
WO2008150461A2 (en) 2008-12-11
CA2857507A1 (en) 2008-12-11
SG178735A1 (en) 2012-03-29
CN101835901B (en) 2013-03-06
EP2171085A4 (en) 2010-07-14
WO2008150463A3 (en) 2009-02-19
CN101896603A (en) 2010-11-24
WO2008150463A2 (en) 2008-12-11
US8669059B2 (en) 2014-03-11
IL202349A0 (en) 2011-08-01
NZ581472A (en) 2012-02-24
ATE540112T1 (en) 2012-01-15
US8318436B2 (en) 2012-11-27
EP2171085A2 (en) 2010-04-07
JP2015037424A (en) 2015-02-26
US20120309014A1 (en) 2012-12-06
EP2463370A1 (en) 2012-06-13
BRPI0812042A2 (en) 2014-10-14
AU2008260557A1 (en) 2008-12-11
US20090253169A1 (en) 2009-10-08
US20120107853A1 (en) 2012-05-03
CA2857507C (en) 2015-03-17
CA2692893A1 (en) 2008-12-11
DK2162537T3 (en) 2012-04-23
CN101835901A (en) 2010-09-15

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