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EP2121682A1 - Dérivés de 4-(pyrrolopyridinyl)-pyrimidin-2-yl-amine - Google Patents

Dérivés de 4-(pyrrolopyridinyl)-pyrimidin-2-yl-amine

Info

Publication number
EP2121682A1
EP2121682A1 EP08707340A EP08707340A EP2121682A1 EP 2121682 A1 EP2121682 A1 EP 2121682A1 EP 08707340 A EP08707340 A EP 08707340A EP 08707340 A EP08707340 A EP 08707340A EP 2121682 A1 EP2121682 A1 EP 2121682A1
Authority
EP
European Patent Office
Prior art keywords
salts
solvates
formula
stereoisomers
tautomers
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP08707340A
Other languages
German (de)
English (en)
Other versions
EP2121682B1 (fr
Inventor
Dieter Dorsch
Christian Sirrenberg
Thomas J. J. Mueller
Eugen Merkul
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Merck Patent GmbH
Original Assignee
Merck Patent GmbH
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Publication of EP2121682A1 publication Critical patent/EP2121682A1/fr
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D471/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00
    • C07D471/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, at least one ring being a six-membered ring with one nitrogen atom, not provided for by groups C07D451/00 - C07D463/00 in which the condensed system contains two hetero rings
    • C07D471/04—Ortho-condensed systems
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/33—Heterocyclic compounds
    • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
    • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
    • A61K31/4353—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems
    • A61K31/437—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom ortho- or peri-condensed with heterocyclic ring systems the heterocyclic ring system containing a five-membered ring having nitrogen as a ring hetero atom, e.g. indolizine, beta-carboline
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/02—Antineoplastic agents specific for leukemia
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A61P35/04—Antineoplastic agents specific for metastasis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

Definitions

  • the invention relates to compounds of the formula
  • R 1 is H 1 A, - [C (R 6 ) 2 ] ⁇ Ar, - [C (R 6 ) 2 ] n Het or
  • R 3 , R 4 are each independently H 1 A, Hal, CN, - [C (R 6 ) 2 ] n Ar, - [C (R 6 ) 2 ] n Het or - [C (R 6 ) 2 ] n cycloalkyl .
  • R 6 is H or alkyl of 1-6 C atoms, A 1 A 1 are each independently of one another unbranched or branched alkyl having 1-10 C atoms, in which one or two
  • CH CH groups and / or also 1-7 H atoms can be replaced by F
  • Ar is an unsubstituted or mono-, di-, tri-, tetra- or quintuplet OH, OA, SH, SA, SOA, SO 2 A, Hal, NO 2 , NH 2 ,
  • CONHA CONAA 1 , NACOA 1 , NASO 2 A 1 , COOH 1 COOA, COA 1 CHO and / or CN substituted saturated, unsaturated or aromatic carbocycle having 5-14 C atoms,
  • Atoms which are unsubstituted or mono- or disubstituted by OH, OA 1 SOA, SO 2 A, Hal 1 NO 2 , NH 2 , NHA, NAA 1 , A 1 SO 2 NH 2 , SO 2 NHA, SO 2 NAA 1 , CONH 2 , CONHA 1 CONAA 1 , NACOA ', 10 NASO 2 A 1 , COOH, COOA, CHO 1 COA and / or CN may be substituted, n is O, 1 or 2, * c and their pharmaceutically usable derivatives, solvates, salts
  • Compounds can therefore be used to control and / or treat tumors, tumor growth and / or tumor metastases.
  • the antiproliferative effect can be demonstrated in a proliferation assay / 5
  • the compounds of the invention or a pharmaceutically acceptable salt thereof are administered for the treatment of cancer including solid carcinomas such as carcinomas (eg, the lungs, pancreas, thyroid, bladder, or colon), myeloid diseases (eg myeloid leukemia) or adenomas (eg villous colonic adenoma).
  • solid carcinomas such as carcinomas (eg, the lungs, pancreas, thyroid, bladder, or colon)
  • myeloid diseases eg myeloid leukemia
  • adenomas eg villous colonic adenoma
  • Tumors also include monocytic leukemia, brain, and
  • Genitourinary, lymphatic, gastric, laryngeal and lung carcinomas including lung adenocarcinoma and small cell lung carcinoma, pancreatic and / or mammary carcinoma.
  • the compounds are also useful in the treatment of HIV-1 (human immunodeficiency virus type 1) induced immunodeficiency.
  • pancreatic cancer liver cancer, kidney cancer, colorectal cancer, breast cancer, head cancer, cervical cancer, esophageal cancer, gynecological cancer, thyroid cancer, lymphoma, chronic leukemia and acute leukemia.
  • cancerous cell growth is a disease that is an object of the present invention.
  • the present invention therefore relates to compounds according to the invention - A -
  • the compounds according to the invention have an antiproliferative action.
  • the compounds of the invention are administered to a patient with a hyperproliferative
  • C disease administered for.
  • the present compounds are useful for prophylactic or
  • Treating is used as a reference to both the prevention of disease and the treatment of pre-existing conditions.
  • the prevention of proliferation / vitality is achieved by administering the
  • the 25 compounds of the invention achieved before developing the obvious disease, z. To prevent tumor growth.
  • the compounds are used to treat persistent diseases by stabilizing or ameliorating the clinical symptoms of the patient
  • the host or patient may be of any mammalian species, e.g. A primate species, especially humans; Including rodents
  • mice Mice, rats and hamsters; Rabbits; Horses, cattle, dogs,
  • Animal models are for experimental studies of Interest, providing a model for the treatment of human disease.
  • the susceptibility of a particular cell to treatment with the compounds of the invention can be determined by testing in vitro. Typically, a culture of the cell is incubated with a compound of the invention at various concentrations for a period of time sufficient to allow the active agents to induce cell death or to inhibit cell proliferation, cell vitality or migration, usually between about one hour and one week. For testing in vitro, cultured cells from a biopsy sample can be used. The amount of cells remaining after treatment are then determined.
  • the dose will vary depending on the specific compound used, the specific disease, the patient status, etc. Typically, one therapeutic dose will be sufficient to treat the undesired one
  • Treatment is generally continued until there is a significant reduction, e.g. B. at least about 50% reduction in cell load and can be continued until essentially no more unwanted cells are detected in the body.
  • the compounds of the present invention are useful in the treatment of a variety of conditions in which proliferation and / or migration of smooth muscle cells and / or inflammatory cells into the intimal layer of a vessel results in limited blood flow to that vessel, e.g. In neointimal occlusive lesions. Too occlusive
  • Transplant vascular diseases of interest include atherosclerosis, coronary vascular disease after transplantation, vein graft stenosis, peri-anastomotic prosthetic restenosis, restenosis after angioplasty or stent placement, and the like.
  • the invention also relates to the optically active forms (stereoisomers), salts, the enantiomers, the racemates, the diastereomers and the hydrates and solvates of these compounds.
  • Solvates of the compounds are understood to mean additions of inert solvent molecules to the compounds which form due to their mutual attraction. Solvates are e.g.
  • compositions are understood, for example, as the salts of the compounds according to the invention as well as so-called prodrug compounds.
  • biodegradable polymer derivatives of the compounds of the invention include biodegradable polymer derivatives of the compounds of the invention, as z. In Int. J. Pharm. 115, 61-67 (1995).
  • the term "effective amount” means the amount of a drug or pharmaceutical agent which elicits a biological or medical response in a tissue, system, animal or human, e.g. sought or desired by a researcher or physician.
  • terapéuticaally effective amount means an amount that, compared to a corresponding subject, this
  • Quantity has not resulted in: improved treatment, cure, prevention or elimination of a
  • Reduction of the progression of a disease, a disease or a disorder Reduction of the progression of a disease, a disease or a disorder.
  • terapéuticaally effective amount also includes the
  • the invention also provides the use of mixtures of the compounds of formula I, e.g. Mixtures of two diastereomers, e.g. in the ratio 1: 1, 1: 2, 1: 3, 1: 4, 1: 5, 1: 10, 1: 100 or 1: 1000. These are particularly preferably mixtures of stereoisomeric compounds.
  • the invention relates to the compounds of the formula I and their
  • R 2 is an indole-protecting group
  • R 3 , R 4 and R 5 have the meanings given in claim 1, 008/000634
  • R 1 has the meaning given in claim 1,
  • R 2 , R 3 , R 4 and R 5 have the meanings given in claim 1,
  • A, A 1 each independently of one another, is unbranched (linear) or branched, and has 1, 2, 3, 4, 5, 6, 7, 8, 9 or 10 C atoms.
  • A is preferably methyl, furthermore ethyl, propyl, isopropyl, butyl, isobutyl, sec. Butyl or tert-butyl, and also pentyl, 1-, 2- or 3-methylbutyl, 1, 1-, 1, 2- or 2,2-dimethylpropyl, 1-ethylpropyl, hexyl, 1-, 2-, 3- or 4-methylpentyl, 1, 1-, 1, 2-, 1, 3-,
  • Atoms preferably methyl, ethyl, propyl, isopropyl, butyl, isobutyl, 008/000634
  • A also means 2-
  • Cycloalkyl is preferably cyclopropyl, cyclobutyl, cyclopentyl, cyclohexyl or cycloheptyl.
  • a saturated, unsaturated or aromatic carbocycle having 5-14 C atoms is preferably cyclopentyl, cyclohexyl, cycloheptyl, phenyl, naphthyl, biphenyl or tetrahydronaphthy I.
  • Ar means e.g. Phenyl, o-, m- or p-tolyl, o-, m- or p-ethylphenyl, o-, m- or p-propylphenyl, o-, m- or p-isopropylphenyl, o-, m- or p- tert-butylphenyl, o-, m- or p-trifluoromethylphenyl, o-, m- or p-fluorophenyl, o-, m- or p-bromophenyl, o-, m- or p-chlorophenyl, o-, m- or p-
  • Ar is preferably an unsubstituted or mono-, di-, tri- or pentasubstituted by OH, OA, NH 2 , NHA, NAA 1 , Hal and / or A. substituted saturated, unsaturated or aromatic carbocycle having 6-14 C atoms.
  • Ar is particularly preferably unsubstituted or mono-, di- or trisubstituted by OH, OA, NH 2 , NHA, NAA 1 , Hal and / or A-substituted phenyl.
  • Isoindolyl 1-, 2-, 4- or 5-benzimidazolyl, 1-, 2-, 3-, 4-, 5-, 6- or 7-
  • Benzoxazolyl 3-, A-, 5-, 6- or 7- benzisoxazolyl, 2-, 4-, 5-, 6- or 7- benzothiazolyl, 2-, 4-, 5-, 6- or 7-benzisothiazolyl, 4-, 5-, 6- or 7-benz-2,1,3-oxadiazolyl, 2-, 3-, 4-, 5-, 6-, 7- or 8-quinolyl, 1-, 3-, 4 -, 5-, 6-, 7-
  • 25 or 8-isoquinolyl 3-, 4-, 5-, 6-, 7- or 8-cinnolinyl, 2-, 4-, 5-, 6-, 7- or 8-quinazolinyl, 5- or 6-quinoxalinyl , 2-, 3-, 5-, 6-, 7- or 8-2H-benzo [1,4] oxazinyl, more preferably 1,3-benzodioxol-5-yl, 1,4-benzodioxan-6-yl, 2,1,3-benzothiadiazol-4 or 5-yl or 2,1,3-benzene
  • the heterocyclic radicals may also be partially or completely hydrogenated.
  • Unsubstituted Het can thus z.
  • B. also mean 2,3-dihydro-2-, -3-, -A- or -5-furyl, 2,5-dihydro-2-, -3-, -A- or 5-furyl, tetrahydro-2 - Or 3-
  • Het is preferably an unsubstituted mono- or binuclear aromatic heterocycle having 1 to 4 N, O and / or S atoms.
  • Het is particularly preferably unsubstituted or mono- or disubstituted by OH 1 OA, Hal and / or A substituted furyl, thienyl, pyrrolyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, pyridyl, pyrimidinyl, triazolyl, tetrazolyl, thiadiazole, pyridazinyl, pyrazinyl Indolyl, isoindolyl, benzimidazolyl, indazoiyl, quinolyl or 1, 3-benzodioxolyl; most preferably furyl, thienyl, pyrrolyl, imidazolyl, pyrazolyl, oxazolyl, isoxazolyl, thiazolyl, pyridyl or pyrimidinyl.
  • R 1 is preferably H, A, - [C (R 6 ) 2 ] n Ar or - [C (R 6 ) 2 ] n Het.
  • R 2 is preferably H.
  • R 3 is preferably H or A.
  • R 4 is preferably H.
  • R 5 is preferably H.
  • R 6 is preferably H.
  • Hal preferably denotes F, Cl or Br, but also I 1 particularly preferably F or Cl.
  • the compounds of the formula I can possess one or more chiral centers and therefore occur in different stereoisomeric forms.
  • Formula I encompasses all these forms.
  • R 1 is H, A, - [C (R 6 ) 2 ] n Ar or - [C (R 6 ) 2 ] n Het;
  • Ig A is unbranched or branched alkyl having 1-6 C atoms, wherein 1-7 H atoms may be replaced by F; 10 in Ih Het is an unsubstituted mono- or binuclear aromatic heterocycle having 1 to 4 N, O and / or S atoms,, c;
  • Isoxazolyl, thiazolyl, pyridyl or pyrimidinyl, 3 represents 0 ;
  • R 3 is H or A
  • R 5 H, R 6 is H or alkyl having 1-6 C atoms
  • A, A 1 are each independently of one another unbranched or branched alkyl having 1-6 C atoms, in which 1-7 H atoms may be replaced by F, Ar is unsubstituted or mono-, di- or trisubstituted by
  • the compounds of the formula I and also the starting materials for their preparation are prepared by methods known per se, as described in the literature (eg in the standard works such as Houben-Weyl, Methoden der organischen Chemie, Georg-Thieme-Verlag, Stuttgart), under reaction conditions which are known and suitable for the mentioned reactions.
  • the compounds of the formula II and of the formula III are generally known. If they are new, they can be produced by methods known per se.
  • the reaction takes place in an inert solvent and takes place in the
  • an acid-binding agent preferably an organic base such as DIPEA, triethylamine, dimethylaniline, pyridine or quinoline.
  • an alkali or alkaline earth metal hydroxide, carbonate or bicarbonate or other salt of a weak acid of the alkali or alkaline earth metals preferably of potassium, sodium, calcium or cesium may be beneficial.
  • reaction time depending on the conditions used, between a few minutes and 14 days, the reaction temperature between about -15 ° and 150 °, normally between 40 ° and 130 °, particularly preferably between 60 ° and 110 0 C.
  • Suitable inert solvents are e.g. Hydrocarbons such as hexane, petroleum ether, benzene, toluene or xylene; chlorinated hydrocarbons such as trichlorethylene, 1, 2-dichloroethane, carbon tetrachloride, chloroform or dichloromethane; Alcohols such as methanol, ethanol, isopropanol, n-propanol, n-butanol or tert-butanol; Ethers, such as diethyl ether, diisopropyl ether, tetrahydrofuran (THF) or dioxane; Glycol ethers, such as ethylene glycol monomethyl or monoethyl ether (methyl glycol or ethyl glycol), ethylene glycol dimethyl ether (diglyme); Ketones such as acetone or butanone; Amides such as acetamide, dimethylacetamide or dimethylformamide (DM
  • Nitromethane or nitrobenzene are Nitromethane or nitrobenzene; Esters such as ethyl acetate or mixtures of said solvents. Particularly preferred are glycol ethers, THF, dichloromethane and / or DMF.
  • Preferred indole protecting groups are e.g. Sulfonyl protecting groups, such as tosyl or mesyl, and further protecting groups, e.g. BOC.
  • Compounds of the formula I can furthermore be obtained by reacting compounds of the formula III with compounds of the formula IV.
  • the compounds of formula IV are known in the rule. If they are new, they can be produced by methods known per se.
  • the reaction takes place in an inert solvent and is generally carried out in the presence of an acid-binding agent, preferably an organic base such as DIPEA, triethylamine, dimethylaniline, pyridine or quinoline.
  • Alkali or alkaline earth metals preferably potassium, sodium,
  • reaction time depending on the conditions used, between a few minutes and 14 days, the reaction temperature between about -15 ° and 150 °, normally between 40 ° and 120 °, particularly preferably between 60 ° and 110 0 C.
  • Suitable inert solvents are the abovementioned.
  • a standard method for ether cleavage e.g. a methyl ether, is the
  • Hydrogenolytically removable groups e.g. the cleavage of a benzyl ether, z. B. by treatment with hydrogen in the presence of a
  • Catalyst eg., A noble metal catalyst such as palladium, expediently on a support such as coal
  • Catalyst are split off.
  • Solvents are the abovementioned, in particular z.
  • alcohols such as methanol or ethanol or amides such as DMF.
  • the hydrogenolysis is usually carried out at temperatures between about 0 and
  • Esters can e.g. be saponified with acetic acid or with NaOH or KOH in water, water-THF or water-dioxane at temperatures between 0 and 100 °.
  • Alkylations on the nitrogen are carried out under standard conditions, as known to those skilled in the art.
  • the compounds of formula I can be further obtained by liberating them from their functional derivatives by solvolysis, in particular hydrolysis, or by hydrogenolysis.
  • Preferred starting materials for the solvolysis or hydrogenolysis are those which contain, instead of one or more free amino and / or hydroxyl groups, corresponding protected amino and / or hydroxyl groups, preferably those which, instead of an H atom which is substituted by an N- Atom, carry an amino protecting group, z.
  • R 1 represents an amino-protecting group, e.g., BOC or CBZ.
  • the protecting groups present are different from each other, they can be selectively cleaved in many cases.
  • amino protecting group is well known and refers to groups which are capable of protecting (blocking) an amino group from chemical reactions, but which are readily removable after the desired chemical reaction has been carried out elsewhere in the molecule. Typical of such groups are in particular unsubstituted or substituted acyl, aryl, aralkoxymethyl or aralkyl groups. Moreover, because the amino protecting groups are removed after the desired reaction (or reaction sequence), their type and size is not critical; however, preference is given to those having 1-20, in particular 1-8 C atoms.
  • acyl group is to be understood in the broadest sense in the context of the present process.
  • acyl groups derived from aliphatic, araliphatic, aromatic or heterocyclic carboxylic acids or sulfonic acids, and in particular alkoxycarbonyl, aryloxycarbonyl and especially aralkoxycarbonyl groups.
  • acyl groups are alkanoyl such as acetyl, propionyl, butyryl; Aralkanoyl such as phenylacetyl; Aroyl such as benzoyl or toluyl; Aryloxyalkanoyl such as POA; Alkoxycarbonyl such as methoxycarbonyl, ethoxycarbonyl, 2,2,2-trichloroethoxycarbonyl, BOC, 2-iodoethoxycarbonyl; Aralkyloxycarbonyl such as CBZ ("carbobenzoxy"), 4-methoxybenzyloxycarbonyl, FMOC; Arylsulfonyl such as Mtr, Pbf or Pmc.
  • Preferred amino protecting groups are BOC and Mtr, furthermore CBZ, Fmoc, benzyl and acetyl.
  • hydroxy protecting group is also well known and refers to groups which are suitable for protecting a hydroxy group from chemical reactions, but which are readily removable after the desired chemical reaction has been carried out at other sites on the molecule. Typical of such groups are the abovementioned unsubstituted or substituted aryl, aralkyl or acyl groups, and also alkyl groups. The nature and size of the hydroxy-protecting groups is not critical since they are removed after the desired chemical reaction or reaction sequence; are preferred
  • hydroxy Protecting groups include tert-butoxycarbonyl, benzyl, p-nitrobenzoyl, p-toluenesulfonyl, tert-butyl and acetyl, with benzyl and tert-butyl being particularly preferred.
  • the COOH groups in aspartic acid and glutamic acid are preferably protected in the form of their tert-butyl esters (eg, Asp (OBut)).
  • Suitable inert solvents are preferably organic, for example carboxylic acids such as acetic acid, ethers such as tetrahydrofuran or dioxane, amides such as DMF, halogenated hydrocarbons such as dichloromethane, and also alcohols such as methanol, ethanol or isopropanol, and water. Also suitable are mixtures of the abovementioned solvents. TFA is preferably used in excess without the addition of another solvent, perchloric acid in the form of a mixture of acetic acid and 70% perchloric acid in a ratio of 9: 1.
  • the reaction temperatures for the cleavage are suitably between about 0 and about 50 °, preferably between 15 and 30 ° (room temperature).
  • the groups BOC, OBut, Pbf, Pmc and Mtr can, for. B. preferably cleaved with TFA in dichloromethane or with about 3 to 5n HCl in dioxane at 15-30 °, the FMOC group with an about 5- to 50% solution of dimethylamine, diethylamine or piperidine in DMF at 15-30 °.
  • Hydrogenolytically removable protecting groups may e.g. By cleavage with hydrogen in the presence of a catalyst (e.g., a noble metal catalyst such as palladium, conveniently on a support such as carbon).
  • a catalyst e.g., a noble metal catalyst such as palladium, conveniently on a support such as carbon.
  • Suitable solvents are those given above, in particular z.
  • alcohols such as
  • Methanol or ethanol or amides such as DMF Methanol or ethanol or amides such as DMF.
  • the hydrogenolysis is in usually at temperatures between about 0 and 100 ° and pressures between about 1 and 200 bar, preferably at 20-30 ° and 1-10 bar performed. Hydrogenolysis of the CBZ group succeeds z. B. good at 5 to 10% Pd / C in methanol or with ammonium formate (instead of hydrogen) on Pd / C in methanol / DMF at 20-30 °.
  • the abovementioned compounds according to the invention can be used in their final non-salt form.
  • the present invention also encompasses the use of these compounds in the form of their pharmaceutically acceptable salts, which can be derived from various organic and inorganic acids and bases according to procedures known in the art.
  • Pharmaceutically acceptable salt forms of the compounds of formula I are for the most part prepared conventionally. If the compound of the formula I contains a carboxylic acid group, one of its suitable salts can be formed by reacting the compound with a suitable base to give the corresponding base addition salt. Such bases are for
  • Example alkali metal hydroxides including potassium hydroxide, sodium hydroxide and lithium hydroxide; Alkaline earth metal hydroxides such as barium hydroxide and calcium hydroxide; Alkali metal alcoholates, e.g. Potassium ethanolate and sodium propanolate; and various organic bases such as piperidine, diethanolamine and N-methylglutamine.
  • the aluminum salts of the compounds of formula I are also included.
  • acid addition salts can be formed by reacting these compounds with pharmaceutically acceptable organic and inorganic acids, e.g.
  • Hydrogen halides such as hydrogen chloride, hydrogen bromide or hydrogen iodide, other mineral acids and their corresponding salts such as sulfate, nitrate or phosphate and the like, and alkyl and monoarylsulfonates such as ethanesulfonate, toluenesulfonate and
  • Benzenesulfonate as well as other organic acids and their corresponding salts, such as acetate, trifluoroacetate, tartrate, maleate, succinate, Citrate, benzoate, salicylate, ascorbate and the like.
  • pharmaceutically acceptable acid addition salts of the compounds of formula I include the following: acetate, adipate, alginate, arginate, aspartate, benzoate, benzenesulfonate (besylate), bisulfate, bisulfite, bromide, butyrate, camphorate, camphorsulfonate, caprylate, chloride, chlorobenzoate, citrate , Cyclopentane propionate, digluconate, dihydrogen phosphate, dinitrobenzoate, dodecylsulfate, ethanesulfonate, fumarate, galacterate (from mucic acid), galacturonate, glucoheptanoate, gluconate,
  • base salts of the invention include
  • salts are ammonium; the alkali metal salts sodium and potassium, and the alkaline earth metal salts calcium and magnesium.
  • O Q bases include salts of primary, secondary and tertiary amines, substituted amines, including naturally occurring substituted amines, cyclic amines and basic ion exchange resins, eg arginine, betaine, caffeine, chloroprocaine, choline, N, N'-dibenzylethylenediamine
  • Tromethamine Hydrabamine, iso-propylamine, lidocaine, lysine, meglumine, N-methyl-D-glucamine, morpholine, piperazine, piperidine, polyamine resins, procaine, purines, theobromine, triethanolamine, triethylamine, trimethylamine, tripropylamine and tris- (hydroxymethyl) -methylamine ( Tromethamine), but this is not 5
  • 20 include acetate, trifluoroacetate, besylate, citrate, fumarate, gluconate, hemisuccinate, hippurate, hydrochloride, hydrobromide, isethionate, mandelate, meglumine, nitrate, oleate, phosphonate, pivalate, sodium phosphate, stearate, sulfate, sulfosalicylate, tartrate, thiomalate, Tosylate and tromethamine, which is not meant to be limiting.
  • the acid addition salts of basic compounds of formula I are prepared by bringing the free base form with a sufficient amount of the desired acid on contact, causing the formation of the salt in a conventional manner.
  • the free base can be regenerated by contacting the salt form with a base and isolating the free base in a conventional manner.
  • the free base forms differ in some sense from their corresponding salt forms in relation to particular ones
  • the pharmaceutically acceptable base addition salts of the compounds of formula I are formed with metals or amines such as alkali metals and alkaline earth metals or organic amines.
  • metals are sodium, potassium, magnesium and calcium.
  • Preferred organic amines are N, N'-dibenzylethylenediamine, chloroprocaine, choline, diethanolamine, ethylenediamine, N-methyl-D-glucamine and procaine.
  • the base addition salts of acidic compounds of the invention are prepared by contacting the free acid form with a sufficient amount of the desired base to form the salt in a conventional manner.
  • the free acid can be regenerated by contacting the salt form with an acid and isolating the free acid in a conventional manner.
  • the free acid forms in some sense differ from their corresponding salt forms in terms of certain physical properties such as solubility in polar solvents; However, in the context of the invention, the salts otherwise correspond to their respective free acid forms.
  • a compound according to the invention contains more than one group which can form such pharmaceutically acceptable salts, the invention also encompasses multiple salts.
  • Typical multiple salt forms include, for example, bitartrate, diacetate, difumarate, dimeglumine, diphosphate, disodium and trihydrochloride, but this is not intended to be limiting.
  • the pharmaceutically acceptable salt form of the drug can also this 5
  • the active ingredient first conferred a desired pharmacokinetic property that it did not previously possess, and may even positively affect the pharmacodynamics of that agent in terms of its therapeutic efficacy in the body.
  • the invention furthermore relates to medicaments comprising at least one compound of the formula I and / or pharmaceutically usable derivatives, solvates and stereoisomers thereof, including mixtures thereof
  • compositions may be presented in the form of dosage units containing a predetermined amount of active ingredient per unit dose.
  • a unit may, for example, 0.5 mg to
  • dosage units containing a predetermined amount of active ingredient per unit dose are presented.
  • Preferred dosage unit formulations are those containing a daily or partial dose as indicated above or a corresponding fraction thereof
  • O0 active ingredient included. Furthermore, such pharmaceutical
  • compositions can be used for administration
  • Such formulations can be prepared by any method known in the pharmaceutical art 5, for example by using the
  • Active substance with the carrier (s) or excipient (s) is brought together.
  • compositions adapted for oral administration may be administered as separate entities, e.g. Capsules or tablets; Powder or granules; Solutions or suspensions in aqueous or non-aqueous liquids; edible foams or foam foods; or
  • Tablet or capsule the active ingredient component with an oral, not -
  • Starch or mannitol is mixed.
  • a flavor, preservative, dispersant and dye may also be present.
  • O Q capsules are prepared by preparing a powder mix as described above and filling shaped gelatin casings therewith.
  • Lubricants and lubricants such as finely divided silica, talc, magnesium stearate, calcium stearate or polyethylene glycol in solid form can be added to the powder mixture before the filling process.
  • Disintegrants or solubilizers such as agar-agar, calcium carbonate or sodium carbonate may also be added to improve the availability of the drug after ingestion of the capsule.
  • Lubricants and disintegrants as well as dyes are also incorporated into the mixture.
  • Suitable binders include starch, gelatin, natural sugars, e.g. Glucose or beta-lactose, corn sweeteners, natural and synthetic gums, e.g. acacia,
  • tragacanth or sodium alginate carboxymethyl cellulose, polyethylene glycol, waxes, and the like.
  • the lubricants used in these dosage forms include sodium oleate, sodium stearate, magnesium stearate, sodium benzoate, sodium acetate, sodium chloride and the like.
  • a ⁇ include, but are not limited to, starch, methyl cellulose, agar, bentonite, xanthan gum and the like.
  • the tablets are formulated by, for example, preparing a powder mixture, granulating or dry-pressing, adding a lubricant and a disintegrating agent and pressing the whole into tablets.
  • a powder mixture is prepared by dissolving the appropriately comminuted compound with a diluent or a base as described above, and optionally with a binder, e.g. Carboxymethylcellulose, an alginate, gelatin or polyvinylpyrrolidone, a solution diluent, e.g.
  • Paraffin, a resorption accelerator, such as a quaternary salt and / or an absorbent, e.g. Bentonite, kaolin or dicalcium phosphate is mixed.
  • the powder mixture can be granulated by mixing it with a binder, e.g. Syrup, starch paste, Acadia slime or solutions of cellulose or polymer materials wetted and pressed through a sieve.
  • a binder e.g. Syrup, starch paste, Acadia slime or solutions of cellulose or polymer materials wetted and pressed through a sieve.
  • the powder mixture can be run through a tabletting machine to produce non-uniformly shaped lumps which are formed in
  • Granules are broken up.
  • the granules may be greased by addition of stearic acid, a stearate salt, talc or mineral oil to prevent sticking to the tablet molds.
  • the greased mixture is then compressed into tablets.
  • the compounds according to the invention can also be combined with a free-flowing inert carrier and then pressed directly into tablets without carrying out the granulation or dry-pressing steps. 5
  • a transparent or opaque protective layer consisting of a shellac sealant, a layer of sugar or polymeric material, and a glossy layer of wax may be present. Dyes can be added to these coatings in order to distinguish between different dosage units.
  • Oral fluids such as solution, syrups and elixirs may be prepared in unit dosage form such that a given. j 5 quantity contains a given amount of compound.
  • Syrups can be prepared by dissolving the compound in an appropriate taste aqueous solution while preparing elixirs using a non-toxic alcoholic vehicle.
  • Suspensions may be prepared by dispersing the compound in a non-aqueous solution.
  • Solubilizers and emulsifiers e.g. ethoxylated isostearyl alcohols and polyoxyethylene sorbitol ethers, preservatives, flavoring additives such as e.g. Peppermint oil or natural sweeteners or saccharin or other artificial sweeteners, i.a. 25 can also be added.
  • the unit dosage formulations for oral administration may optionally be encapsulated in microcapsules.
  • the formulation O0 can also be prepared so that the release is prolonged or retarded, such as by coating or embedding of particulate material in polymers, wax, etc.
  • 35 functional derivatives thereof can also be in the form of liposome delivery systems, such as small unilamellar vesicles, large unilamellar vesicles and multilamellar vesicles.
  • Liposomes can be formed from various phospholipids such as cholesterol, stearylamine or phosphatidylcholines.
  • the compounds of formula I as well as the salts, solvates and physiologically functional derivatives thereof can also be delivered using monoclonal antibodies as individual carriers to which the compound molecules are coupled.
  • the compounds can also be coupled with soluble polymers as targeted drug carriers.
  • Such polymers may include polyvinylpyrrolidone, pyran copolymer, polyhydroxypropylmethacrylamidephenol, polyhydroxyethylaspartamidophenol or polyethyleneoxidepolylysine substituted with palmitoyl radicals.
  • the compounds can be attached to a class of biodegradable polymers suitable for the controlled release of a drug, e.g.
  • Polylactic acid Polyepsilon-caprolactone, polyhydroxybutyric acid, polyorthoesters, polyacetals, polydihydroxy-pyrans, polycyanoacrylates, and crosslinked or amphipathic block copolymers of hydrogels.
  • compositions adapted for transdermal administration may be presented as discrete patches for prolonged, intimate contact with the epidermis of the recipient.
  • the drug may be delivered from the patch by iontophoresis as generally described in Pharmaceutical Research, 3 (6), 318 (1986).
  • Pharmaceutical compounds adapted for topical administration may be formulated as ointments, creams, suspensions, lotions, powders, solutions, pastes, gels, sprays, aerosols or oils.
  • the formulations are preferably used as a topical ointment EP2008 / 000634
  • the active ingredient can be used with either a paraffinic or water miscible cream base.
  • the active ingredient can be formulated into a cream with an oil-in-water cream base or a water-in-oil base.
  • the pharmaceutical formulations adapted for topical application to the eye include eye drops wherein the active ingredient is dissolved or suspended in a suitable carrier, especially an aqueous solvent.
  • compositions adapted for topical application in the mouth include lozenges, troches and mouthwashes.
  • compositions adapted for rectal administration may be presented in the form of suppositories or enemas.
  • compositions adapted for nasal administration in which the vehicle is a solid contain a coarse powder having a particle size, for example, in the range of 20-500 microns, which is administered in the manner in which snuff is received, i. by rapid inhalation via the nasal passages from a container held close to the nose with the powder.
  • Suitable formulations for administration as a nasal spray or nasal drops with a liquid carrier include drug solutions in water or oil.
  • compositions adapted for administration by inhalation include fine particulate dusts or nebulas containing various types of pressurized dosing dispensers
  • Aerosols, nebulizers or insufflators can be generated.
  • Pharmaceutical formulations adapted for vaginal administration may be presented as pessaries, tampons, creams, gels, pastes, foams or spray formulations.
  • Formulations include aqueous and non-aqueous sterile injection solutions containing the antioxidants, buffers, bacteriostats and solutes, by which the formulation is isotonic with the blood of the treatment
  • aqueous and non-aqueous sterile suspensions which may contain suspending agents and thickeners.
  • the formulations may be administered in single or multiple dose containers, e.g. sealed ampoules and vials, presented
  • injection solutions and suspensions prepared by formulation can be prepared from sterile powders, granules and tablets.
  • formulations may include other means conventional in the art with respect to the particular type of formulation; for example, formulations suitable for oral administration may contain flavorings.
  • a therapeutically effective amount of a compound of formula I 30 is suspended on a number of factors, including, for example, the age and
  • Weight of the animal the exact disease state that requires treatment, and its severity, the nature of the formulation and the route of administration, and is ultimately determined by the attending physician or veterinarian. However, an effective amount is one
  • Growth eg colon or breast carcinoma, generally in the area from 0.1 to 100 mg / kg of body weight of the recipient (mammal) per day, and more typically in the range of 1 to 10 mg / kg of body weight per day.
  • the actual amount per day would usually be between 70 and 700 mg, this amount being given as a single dose per day or more commonly in a number of divided doses (such as two, three, four, five or six) per Day can be given so that the total daily dose is the same.
  • An effective amount of a salt or solvate or a physiologically functional derivative thereof can be determined as a proportion of the effective amount of the compound of the invention per se. It can be assumed that similar dosages are suitable for the treatment of the other, above-mentioned disease states.
  • the invention furthermore relates to medicaments comprising at least one compound of the formula I and / or pharmaceutically usable derivatives, solvates and stereoisomers thereof, including mixtures thereof in all ratios, and at least one further active pharmaceutical ingredient.
  • the invention is also a set (kit), consisting of separate packages of
  • the kit contains suitable containers, such as boxes or boxes, individual bottles, bags or ampoules.
  • suitable containers such as boxes or boxes, individual bottles, bags or ampoules.
  • the set may e.g. separate ampoules containing, in each case, an effective amount of a
  • the instant compounds are useful as pharmaceutical agents for mammals, particularly for humans, in the treatment and control of cancers.
  • the present invention includes the use of the compounds of
  • Formel I and / or their physiologically acceptable salts and solvates for the manufacture of a medicament for the treatment or prevention of
  • Group Himkarzinom urogenital tract carcinoma, carcinoma of the lymphatic system, gastric carcinoma, laryngeal carcinoma and lung carcinoma colon cancer.
  • Another group of preferred forms of cancer are monocytic leukemia, lung adenocarcinoma, small cell lung carcinoma, pancreatic cancer, glioblastoma and breast carcinoma.
  • the compounds of formula I and / or their physiologically acceptable salts and solvates for the manufacture of a medicament for the treatment and / or control of a tumorous disease in a mammal, which method is given to a diseased mammal receiving such treatment required, administered a therapeutically effective amount of a compound of the invention.
  • the therapeutic amount depends on the particular disease and can be determined by the skilled person without great effort.
  • the solid tumor is preferably selected from the group of squamous cell tumors, bladder, stomach, kidney, head and neck, esophagus, cervix, thyroid, intestine, liver, brain, prostate, Urogenital tract, the lymphatic system, the stomach, the larynx and / or the lungs.
  • the solid tumor is furthermore preferably selected from the group of lung adenocarcinoma, small cell lung carcinoma, pancreatic cancer, glioblastoma, colon carcinoma and breast carcinoma.
  • a tumor of the blood and immune system preferably for the treatment of a tumor ⁇ 5 is selected from the group of acute myeloid leukemia, chronic myeloid leukemia, acute lymphatic leukemia and / or chronic lymphatic leukemia.
  • the invention furthermore relates to the use of the invention
  • the compounds of Formula I may also be coadministered with other well-known therapeutics selected for their particular suitability for the condition being treated.
  • the present compounds are also suitable for combination with
  • anticancer agents include the following: estrogen receptor modulators, androgen receptor modulators, retinoid receptor modulators, cytotoxic agents, antiproliferative agents, prenyl-protein transferase inhibitors, HMG-CoA reductase inhibitors,
  • HIV protease inhibitors HIV protease inhibitors, reverse transcriptase inhibitors and others
  • Angiogenesis inhibitors are particularly suitable for co-administration with radiotherapy.
  • Estrogen receptor modulators refers to compounds that interfere with or inhibit the binding of estrogen to the receptor, regardless of how this occurs.
  • the estrogen receptor modulators include, for example, tamoxifen, raloxifene, idoxifen,
  • “Androgen receptor modulators” refers to compounds that interfere with or inhibit the binding of androgens to the receptor, regardless of how this happens
  • ⁇ C androgen receptor modulators include, for example, finasteride and other 5 ⁇ -reductase inhibitors, nilutamide, flutamide, bicalutamide, liarozole and abiraterone acetate.
  • Retinoid receptor modulators refers to compounds that interfere with or inhibit the binding of retinoids to the receptor
  • Such retinoid receptor modulators include, for example, bexarotene, tretinoin, 13-cis retinoic acid, 9-cis retinoic acid, ⁇ -difluoromethylornithine, ILX23-7553, trans-N- (4'-hydroxyphenyl) retinamide, and N-4 carboxyphenyl.
  • Cytotoxic agents refers to compounds which cause cell death or interfere with cell myosis, primarily by direct action on cell function, including alkylating agents, tumor necrosis factors, intercalators, microtubulin inhibitors and topoisomerase
  • the cytotoxic agents include, for example, tirapazimine, Sertenef, cachectin, ifosfamide, tasonermine, lonidamine, carboplatin, altretamine, prednimustine, dibromodulcite, ranimustine, fotemustine, nedaplatin, oxaliplatin,
  • Temozolomide Temozolomide, heptaplatin, estramustine, improvisulfan-tosylate, trofosfamide,
  • Idarubicin Idarubicin, daunorubicin, bisantrene, mitoxantrone, pirarubicin, pinafid,
  • microtubulin inhibitors include, for example, paclitaxel, vindesine sulfate, S '' '- dideshyr''-deoxy- ⁇ '-norvincaleukoblastin, docetaxol, rhizoxin, dolastatin, mivobulin isethionate, auristatin, cemadotin,
  • RPR109881, BMS184476 vinflunine, cryptophycin, 2,3,4,5,6-pentafluoro-N- (3-fluoro-4-methoxyphenyl) benzenesulfonamide, anhydrovinblastine, N 1 N-dimethyl-L-valyl-L-valyl-N methyl-L-valyl-L-prolyl-L-proline-t-butylamide,
  • Topoisomerase inhibitors are for example topotecan, hycaptamine,
  • Antiproliferative agents include antisense RNA and DNA oligonucleotides such as G3139, ODN698, RVASKRAS, GEM231 and INX3001, and antimetabolites such as enocitabine, carmofur, tegafur, pentostatin, doxifluridine, trimetrexate, fludarabine, capecitabine, galocitabine, cytarabine. ocfosfat, Fosteabin Sodium Hydrate, Raltitrexed, Paltitrexide,
  • antiproliferative agents also include other monoclonal antibodies to growth factors already mentioned among the angiogenesis inhibitors, such as trastuzumab, as well as tumor suppressor genes, such as p53, which can be delivered via recombinant virus-mediated gene transfer (see, eg, US Patent No. 6,069,134 ).
  • the present description of the experiment describes the inhibition of tumor cell proliferation / tumor cell vitality by active ingredients.
  • the cells are seeded in suitable cell density in microtiter plates (96-well format) and the test substances are added in the form of a concentration series. After four more days of culture in serum-containing medium, tumor cell proliferation / tumor cell vitality can be determined by means of an Alamarblue test system. 0
  • colon carcinoma cell lines For example, commercially available colon carcinoma cell lines, ovarian cell lines, prostate cell lines, or breast cell lines, etc.
  • the cells are cultured in medium. At intervals of several
  • the cells are cultured at 37 ° C and 10% CO 2 . 5 2.2. Sowing the cells
  • a defined number of cells for example 2000 cells are seeded per culture / well in a volume of 180 ⁇ l of culture medium in microtiter plates (96-well cell culture plates) with a multichannel pipette. The cells are then cultured in a CO2 incubator (37 ° C and 10% CO2).
  • test substances g The test substances are dissolved, for example, in DMSO and then in a corresponding concentration (if appropriate a T / EP2008 / 000634
  • Dilution series in the cell culture medium.
  • the dilution steps can be adjusted depending on the efficiency of the active ingredients and the desired spread of the concentrations.
  • the test substances are mixed in appropriate concentrations with cell culture medium.
  • the plates can be right in front of the 0
  • APCI-MS atmospheric pressure chemical ionization - mass spectrometry (M + H) + .
  • Chromolith Performance RP-18e Merck KGaA, Cat 1.02129.0001
  • Example A Injection glasses
  • a solution of 100 g of an active compound of the formula I and 5 g of disodium hydrogen phosphate is adjusted to pH 6.5 in 3 l bidistilled water with 2 N hydrochloric acid, sterile filtered, filled into injection jars, lyophilized under sterile conditions and sealed sterile. Each injection jar contains 5 mg of active ingredient.
  • each suppository contains 20 mg of active ingredient.
  • a solution of 1 g of an active compound of the formula I, 9.38 g of NaH 2 PO 4 • 2H 2 O, 28.48 g of Na 2 HPO 4 • 12H 2 O and 0.1 g of benzalkonium chloride in 940 is prepared ml of double distilled water. Adjust to pH 6.8, make up to 1 liter and sterilize by irradiation. This solution can be used in the form of eye drops.
  • 500 mg of an active compound of the formula I are mixed with 99.5 g of Vaseline under aseptic conditions.
  • a mixture of 1 kg of active ingredient of the formula I, 4 kg of lactose, 1, 2 kg of potato starch, 0.2 kg of talc and 0.1 kg of magnesium stearate is compressed in the usual way to form tablets, such that each tablet contains 10 mg of active ingredient ,
  • Tablets are pressed analogously to Example E, which are then coated in the usual way with a coating of sucrose, potato starch, talc, tragacanth and dye.
  • a solution of 1 kg of active compound of the formula I in 60 l of bidistilled water is sterile filtered, filled into ampoules, lyophilized under sterile conditions and sealed sterile. Each vial contains 10 mg of active ingredient.

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Abstract

Ces composés de formule (I), où R1, R2, R3, R4 et R5 ont les significations indiquées dans la revendication 1, sont des inhibiteurs de la prolifération cellulaire et de la vitalité cellulaire et peuvent être utilisés dans le traitement de tumeurs.
EP08707340.9A 2007-02-21 2008-01-28 Dérivés de 4-(pyrrolopyridinyl)-pyrimidin-2-yl-amine Not-in-force EP2121682B1 (fr)

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PCT/EP2008/000634 WO2008101587A1 (fr) 2007-02-21 2008-01-28 Dérivés de 4-(pyrrolopyridinyl)-pyrimidin-2-yl-amine

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WO2007140263A2 (fr) * 2006-05-26 2007-12-06 University Of Louisville Research Foundation, Inc. Antagonistes du facteur d'inhibition de la migration des macrophages et leurs procédés d'utilisation
EP2480235A4 (fr) 2009-09-24 2013-05-08 Univ Louisville Res Found Nouveaux dérivés d'iodo pyrimidines utiles pour le traitement de maladies ou affections liées au facteur inhibiteur de la migration des macrophages
US9155790B2 (en) 2010-05-20 2015-10-13 University of Lousiville Research Foundation, Inc. Methods and compositions for modulating ocular damage

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EP2121682B1 (fr) 2013-07-10
US8557977B2 (en) 2013-10-15
ZA200906539B (en) 2010-06-30
EA200901100A1 (ru) 2010-02-26
AR065390A1 (es) 2009-06-03
CA2678691A1 (fr) 2008-08-28
AU2008217297A1 (en) 2008-08-28
US20110092527A1 (en) 2011-04-21
KR20090110948A (ko) 2009-10-23
DE102007008419A1 (de) 2008-08-28
MX2009008864A (es) 2009-08-28
US8293891B2 (en) 2012-10-23
CN101616918A (zh) 2009-12-30
BRPI0808167A2 (pt) 2014-07-08
WO2008101587A1 (fr) 2008-08-28
DK2121682T3 (da) 2013-09-08
JP5508026B2 (ja) 2014-05-28
JP2010519230A (ja) 2010-06-03
IL200470A0 (en) 2010-04-29
ES2427594T3 (es) 2013-10-31
CA2678691C (fr) 2015-09-08
PT2121682E (pt) 2013-10-17
AU2008217297B2 (en) 2013-01-17
IL200470A (en) 2014-02-27

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